[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control soluti...[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control solution, chlorogenic acid control solution and Tangjiangshenkang granule test solution in the range of 200-400 nm. In the ultraviolet scanning diagram of arctiin reference solution, the maximum absorption wavelength of 280 nm was determined as the determination wavelength λ 1, the detection wavelength in the ultraviolet scanning diagram of chlorogenic acid reference solution ( λ 1=280 nm) was determined, and 350 nm was the reference wavelength λ 2;the content of total lignosides in Tangjiangshenkang granules was determined with arctiin as the reference substance. [Results] The precision, accuracy, and durability of this method were fine. The concentration of arctiin was linearly correlated with the absorbance difference in the range of 0.007 95-0.071 55 mg/mL ( r =0. 999 9). The average recovery of arctiin was 100.8%, and the RSD value was 1.04% ( n =6). Calculated as arctiin, three batches of Tangjiangshenkang granules contain no less than 20% of total lignosides. [Conclusions] The method has the advantages of simple operation, good accuracy, precision and reliable stability. It can be used as the content determination and quality control method of total lignosides in Tangjiangshenkang granules.展开更多
In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures w...In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from S.lyraturn and identified as syringin(1),(+)-isolariciresinol(2),(+)-syringaresinol(3),leptolepisol D(4),(-)-secoisolariciresinol(5),(-)-epi-syringaresinol(6),aviculin(7),zhebeiresinol(8),ciwujiatone(9),(-)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxylignan-9′-ol-7-one(10),(+)-lariciresinol(11),(+)-pinoresinol(12)and(+)-medioresinol(13).All the compounds were isolated from S.lyratum for the first time.展开更多
基金Supported by Major Science and Technology Project"Major New Drug Innovation".
文摘[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control solution, chlorogenic acid control solution and Tangjiangshenkang granule test solution in the range of 200-400 nm. In the ultraviolet scanning diagram of arctiin reference solution, the maximum absorption wavelength of 280 nm was determined as the determination wavelength λ 1, the detection wavelength in the ultraviolet scanning diagram of chlorogenic acid reference solution ( λ 1=280 nm) was determined, and 350 nm was the reference wavelength λ 2;the content of total lignosides in Tangjiangshenkang granules was determined with arctiin as the reference substance. [Results] The precision, accuracy, and durability of this method were fine. The concentration of arctiin was linearly correlated with the absorbance difference in the range of 0.007 95-0.071 55 mg/mL ( r =0. 999 9). The average recovery of arctiin was 100.8%, and the RSD value was 1.04% ( n =6). Calculated as arctiin, three batches of Tangjiangshenkang granules contain no less than 20% of total lignosides. [Conclusions] The method has the advantages of simple operation, good accuracy, precision and reliable stability. It can be used as the content determination and quality control method of total lignosides in Tangjiangshenkang granules.
基金Science and Technology Department Plan of Jilin Province,China(Grant No.20160101341JC and 20160622010JC)
文摘In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from S.lyraturn and identified as syringin(1),(+)-isolariciresinol(2),(+)-syringaresinol(3),leptolepisol D(4),(-)-secoisolariciresinol(5),(-)-epi-syringaresinol(6),aviculin(7),zhebeiresinol(8),ciwujiatone(9),(-)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxylignan-9′-ol-7-one(10),(+)-lariciresinol(11),(+)-pinoresinol(12)and(+)-medioresinol(13).All the compounds were isolated from S.lyratum for the first time.
