The objective of our study was to lay a foundation for the effect of flower flagrance on human emotions and to provide a theory for the choice of indoor plants and the improvement of the olfactory environment. Specifi...The objective of our study was to lay a foundation for the effect of flower flagrance on human emotions and to provide a theory for the choice of indoor plants and the improvement of the olfactory environment. Specifically, our purpose was to study human physiological responses to flower fragrance of Lilium ＇Siberia＇ and Rosa ＇Escimo＇. The participants were 31 college students. Blood pressure, pulse rate, finger temperature （FT） and galvanic skin response （GSR） were measured. The results show that the fragrance of Rosa ＇Escimo＇ causes the diastolic pressure and pulse rate of the participants to reduce significantly. The average decrease was 0.37 kPa and 2.23 beats per minute, which indicates that sympathetic nervous activity increases, physiological arousal decreases and emotional alleviation occurs. Furthermore, the GSR of participants significantly increased by smelling the fragrance of Lilium ＇Siberia＇, indicating that both sympathetic nervous activity and physiological arousal increased. But the data could not prove that flower fragrance stimulation has an effect on changes in systolic pressure and finger temperature. Some other factors, such as basic emotion and weather, may have an effect.展开更多
LFY family genes play a conserved role in regulating flowering. In this study, the cDNA of LiLFY1 was isolated with the strategy of RT-PCR in combination with RACE from lily （Lilium longiflorum Thunb.）. LiLFY1 encod...LFY family genes play a conserved role in regulating flowering. In this study, the cDNA of LiLFY1 was isolated with the strategy of RT-PCR in combination with RACE from lily （Lilium longiflorum Thunb.）. LiLFY1 encodes a LFY transcriptional factor. The alignment analysis of the deduced LiLFY1 protein with other known LFY family proteins indicates that LiLFY1 is highly homologous with rice RFL and maize FLL. The result of Southern hybridization showed that there are two copies of LFY family genes in lily. LiLFY1 is expressed in young flower buds and shoot apical meristem （SAM） but not in roots, shoots, mature leaves, and mature floral organs. The cloned LiLFY1 gene may be applied to genetic engineering aiming for regulating the flowering time in lily.展开更多
[Objectives] Allene oxide synthase（ AOS）,the first specific enzyme in the biosynthetic pathway of jasmonate（ JA）,has an important role in regulating JA accumulation in plants. This study was conducted to amplify f...[Objectives] Allene oxide synthase（ AOS）,the first specific enzyme in the biosynthetic pathway of jasmonate（ JA）,has an important role in regulating JA accumulation in plants. This study was conducted to amplify full-length AOS gene from Lilium‘Siberia＇（ LsAOS） and to explore the spatiotemporal expression of this gene. [Methods]LsAOS was cloned by rapid amplification of c DNA ends（ RACE） and analyzed bioinformatically. LsAOS expression level in nine organs/tissues and at four flowering stages was determined by quantitative PCR（ q-PCR）. [Results] LsAOS gene has an open reading frame of 1 542 bp,encoding a protein of 514 amino acids. The LsAOS protein sequence was more homologous with the AOS protein of Musa acuminata Colla than that in any other plant species.The LsAOS expression reached the maximum level at bud stage,and then gradually decreased over time during the flowering period. The LsAOS expression level in leaves was the highest,followed by in inner petals,roots,stems,etc. [Conclusions]JA participates in the whole process of plant development,and is closely related to stress resistance and secondary metabolism. AOS is one of the key genes in JA pathway and plays an important role in regulating the biosynthesis of JA.展开更多
基金supported by the National Key Technology R&D Program of the Eleventh Five-Year Plan of China (2006BAD07B09)
文摘The objective of our study was to lay a foundation for the effect of flower flagrance on human emotions and to provide a theory for the choice of indoor plants and the improvement of the olfactory environment. Specifically, our purpose was to study human physiological responses to flower fragrance of Lilium ＇Siberia＇ and Rosa ＇Escimo＇. The participants were 31 college students. Blood pressure, pulse rate, finger temperature （FT） and galvanic skin response （GSR） were measured. The results show that the fragrance of Rosa ＇Escimo＇ causes the diastolic pressure and pulse rate of the participants to reduce significantly. The average decrease was 0.37 kPa and 2.23 beats per minute, which indicates that sympathetic nervous activity increases, physiological arousal decreases and emotional alleviation occurs. Furthermore, the GSR of participants significantly increased by smelling the fragrance of Lilium ＇Siberia＇, indicating that both sympathetic nervous activity and physiological arousal increased. But the data could not prove that flower fragrance stimulation has an effect on changes in systolic pressure and finger temperature. Some other factors, such as basic emotion and weather, may have an effect.
基金the grant of National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,in Beijing
文摘LFY family genes play a conserved role in regulating flowering. In this study, the cDNA of LiLFY1 was isolated with the strategy of RT-PCR in combination with RACE from lily （Lilium longiflorum Thunb.）. LiLFY1 encodes a LFY transcriptional factor. The alignment analysis of the deduced LiLFY1 protein with other known LFY family proteins indicates that LiLFY1 is highly homologous with rice RFL and maize FLL. The result of Southern hybridization showed that there are two copies of LFY family genes in lily. LiLFY1 is expressed in young flower buds and shoot apical meristem （SAM） but not in roots, shoots, mature leaves, and mature floral organs. The cloned LiLFY1 gene may be applied to genetic engineering aiming for regulating the flowering time in lily.
基金Supported by Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges in Beijing Municipality(DHT20150503)National Natural Science Foundation of China(3120164531640070)
文摘[Objectives] Allene oxide synthase（ AOS）,the first specific enzyme in the biosynthetic pathway of jasmonate（ JA）,has an important role in regulating JA accumulation in plants. This study was conducted to amplify full-length AOS gene from Lilium‘Siberia＇（ LsAOS） and to explore the spatiotemporal expression of this gene. [Methods]LsAOS was cloned by rapid amplification of c DNA ends（ RACE） and analyzed bioinformatically. LsAOS expression level in nine organs/tissues and at four flowering stages was determined by quantitative PCR（ q-PCR）. [Results] LsAOS gene has an open reading frame of 1 542 bp,encoding a protein of 514 amino acids. The LsAOS protein sequence was more homologous with the AOS protein of Musa acuminata Colla than that in any other plant species.The LsAOS expression reached the maximum level at bud stage,and then gradually decreased over time during the flowering period. The LsAOS expression level in leaves was the highest,followed by in inner petals,roots,stems,etc. [Conclusions]JA participates in the whole process of plant development,and is closely related to stress resistance and secondary metabolism. AOS is one of the key genes in JA pathway and plays an important role in regulating the biosynthesis of JA.
