The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious i...The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells.展开更多
Morphological changes during the embryonic development of limbs of the green turtle,Chelonia mydas,were studied during the entire period of incubation,using transmission and scanning electron microscopy(TEM and SEM). ...Morphological changes during the embryonic development of limbs of the green turtle,Chelonia mydas,were studied during the entire period of incubation,using transmission and scanning electron microscopy(TEM and SEM). Limb buds were first observed at Stage 2. At that stage,the tip was covered with an apical ectodermal ridge(AER) which began to regress at Stage 6. Associated with AER was the presence of the mesenchymal cells which,consequently,differentiated into muscles,cartilage and bones. The gross features of the skeletal development appeared as a condensation of the cartilaginous structures in the proximal distal region of the limbs. The primordial digits were gradually enclosed by hard keratinized webbed skin. The increase in rate of ossification and skin pigmentation was correlated with the growth of the limbs. The development of the limbs was closely related to the transitional appearance of mucus secretion from the epidermis.展开更多
Bioactive proteins from SD rat limb buds were extracted and purified. Fractions of 22 ku, 34 ku and 95 ku were proved to have neurotrophic activity to neurons, and the combined activity of these three fractions was th...Bioactive proteins from SD rat limb buds were extracted and purified. Fractions of 22 ku, 34 ku and 95 ku were proved to have neurotrophic activity to neurons, and the combined activity of these three fractions was the highest. So they were combinedly added into the culture medium of sensor neurons in dorsal root ganglia and motor neurons of anterior spinal cord from 2-week-old embryonic rats, and PBS was added as control. Phase-contrast microscopic and electron microscopic observations, and true cholinesterase measurements were performed to evaluate the survival and changes in growth, function, and ultrastructure of these cultured neurons. In the experimental group, it was found that the AchE activity was higher (P<0. 01), ultrastructural changes in mitochondria,Gorgi's complex and other cell organs were milder than those in the control group. The results showed limb buds derived neurotrophins played an important role in maitaining the survival of the neurons and promoting the growth of axons. It was concluded that embryonic limb buds derived neurotrophins had high neurotrophic activities on neurons' survival and axon growth.展开更多
文摘The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells.
文摘Morphological changes during the embryonic development of limbs of the green turtle,Chelonia mydas,were studied during the entire period of incubation,using transmission and scanning electron microscopy(TEM and SEM). Limb buds were first observed at Stage 2. At that stage,the tip was covered with an apical ectodermal ridge(AER) which began to regress at Stage 6. Associated with AER was the presence of the mesenchymal cells which,consequently,differentiated into muscles,cartilage and bones. The gross features of the skeletal development appeared as a condensation of the cartilaginous structures in the proximal distal region of the limbs. The primordial digits were gradually enclosed by hard keratinized webbed skin. The increase in rate of ossification and skin pigmentation was correlated with the growth of the limbs. The development of the limbs was closely related to the transitional appearance of mucus secretion from the epidermis.
基金This project was supported by grants from the Public Health Ministry Research Foundation and Hubei Natural Science Foundation of China (No.97J071)
文摘Bioactive proteins from SD rat limb buds were extracted and purified. Fractions of 22 ku, 34 ku and 95 ku were proved to have neurotrophic activity to neurons, and the combined activity of these three fractions was the highest. So they were combinedly added into the culture medium of sensor neurons in dorsal root ganglia and motor neurons of anterior spinal cord from 2-week-old embryonic rats, and PBS was added as control. Phase-contrast microscopic and electron microscopic observations, and true cholinesterase measurements were performed to evaluate the survival and changes in growth, function, and ultrastructure of these cultured neurons. In the experimental group, it was found that the AchE activity was higher (P<0. 01), ultrastructural changes in mitochondria,Gorgi's complex and other cell organs were milder than those in the control group. The results showed limb buds derived neurotrophins played an important role in maitaining the survival of the neurons and promoting the growth of axons. It was concluded that embryonic limb buds derived neurotrophins had high neurotrophic activities on neurons' survival and axon growth.
