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基于BCD工艺的LIN总线收发器设计
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作者 杨雨辰 王志亮 +1 位作者 孙力 谭庶欣 《固体电子学研究与进展》 CAS 2024年第1期50-58,共9页
针对汽车内部复杂的控制节点与严苛的通讯环境,设计了一款抑制电磁干扰的局域互联网络(Local interconnect network, LIN)总线收发器芯片。基于90 nm BCD高压工艺,采用双向静电放电保护、分段电流驱动、共源级隔离驱动以及总线反馈技术... 针对汽车内部复杂的控制节点与严苛的通讯环境,设计了一款抑制电磁干扰的局域互联网络(Local interconnect network, LIN)总线收发器芯片。基于90 nm BCD高压工艺,采用双向静电放电保护、分段电流驱动、共源级隔离驱动以及总线反馈技术提高系统可靠性。测试结果表明芯片性能符合LIN总线物理层协议规范要求,实现了高低电压域的转换;同时具有良好的抗干扰能力,信号占空比最大变化仅为2.8%,电磁辐射比标准限制值低28 dBμV,达到车用LIN总线通讯相关标准要求。 展开更多
关键词 lin总线 收发器 斜率控制 BCD工艺 电磁干扰 ESD保护
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高可靠LIN控制器IP的设计与实现
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作者 李小波 王祥莉 +2 位作者 赵鹏翔 韩明 林剑东 《现代电子技术》 北大核心 2024年第22期30-36,共7页
LIN虽然为传统总线,但仍大量应用在汽车、智能家居和工业控制等领域。基于此,设计一种高可靠LIN控制器IP。基于AMBA APB3.0标准接口的IP不仅实现了LIN协议规定的数据收发、网络管理等功能,还增加了大量可靠性和安全性相关功能,如错误监... LIN虽然为传统总线,但仍大量应用在汽车、智能家居和工业控制等领域。基于此,设计一种高可靠LIN控制器IP。基于AMBA APB3.0标准接口的IP不仅实现了LIN协议规定的数据收发、网络管理等功能,还增加了大量可靠性和安全性相关功能,如错误监测、看门狗、环回自测试、显性电平监控等,通过这些可靠性措施使得IP达到ASIL-B级技术指标要求。针对IP搭建仿真验证环境,完成了SoC级仿真和FPGA原型验证,并基于国内某流片厂商110 nm工艺,完成了逻辑综合,生成了Verilog格式的网表文件。通过测试和逻辑综合分析得出:所提出的控制器IP性能满足协议和设计需求,在110 nm三温三压极限条件最高频率可达625 MHz,逻辑单元约为5 950,等效逻辑门约为10 554,面积为34 676μm2。该IP功能优于对比文献,可直接集成到微控制器MCU、SoC和FPGA设计中,减少设计周期和产品上市时间。 展开更多
关键词 lin总线 控制器IP 高可靠性 安全功能 逻辑综合 SoC仿真 FPGA原型验证
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LIN28A/B在肿瘤发生发展中的作用研究进展
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作者 张译文 黄琴 +2 位作者 吴艳芸 孙月 韦永龙 《遗传》 CAS CSCD 北大核心 2024年第6期452-465,共14页
LIN28A及其同源蛋白LIN28B是高度保守的RNA结合蛋白,在胚胎早期发育、体细胞重编程、葡萄糖代谢以及肿瘤发生发展中具有重要作用。LIN28A/B在乳腺癌等恶性肿瘤中高表达,在肿瘤的起始、维持和转移中均发挥重要作用,并与预后不良相关。研... LIN28A及其同源蛋白LIN28B是高度保守的RNA结合蛋白,在胚胎早期发育、体细胞重编程、葡萄糖代谢以及肿瘤发生发展中具有重要作用。LIN28A/B在乳腺癌等恶性肿瘤中高表达,在肿瘤的起始、维持和转移中均发挥重要作用,并与预后不良相关。研究表明,LIN28A/B主要通过抑制let-7s等microRNA和直接靶向mRNA的方式发挥调控作用。本文主要综述了LIN28A/B在肝癌、乳腺癌、结直肠癌等恶性肿瘤中的功能及其分子调控机制,以期为进一步研究LIN28A/B的作用机制及其在临床治疗中的可能应用提供理论参考。 展开更多
关键词 lin28A lin28B let-7 miRNA 肿瘤发生发展 治疗靶点
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New findings of Changxingaspis(Xiushuiaspidae,Galeaspida)from the Silurian of Tarim Basin and Zhejiang Province,China
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作者 LI Xutong ZHANG Yumeng +5 位作者 LIN Xianghong ZHU Min ZHAO Wenjin TANG Lizhou SHAN Xianren GAI Zhikun 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第3期531-540,共10页
New discovery of the early Silurian fossil fish Changxingaspis(Xiushuiaspidae,Galeaspida),Changxingaspis nianzhongi sp.nov.and C.gui,are described from the Tataertag Formation in Tarim Basin and the Kangshan Formation... New discovery of the early Silurian fossil fish Changxingaspis(Xiushuiaspidae,Galeaspida),Changxingaspis nianzhongi sp.nov.and C.gui,are described from the Tataertag Formation in Tarim Basin and the Kangshan Formation in Zhejiang Province,respectively.C.nianzhongi mainly differs from C.gui in the shape of the median dorsal opening that is transverse elliptic with a width/length ratio of about 3.0,the long lateral transverse canals extending to the lateral margin of the headshield,and the second lateral transverse canal with dichotomous branchings.Discovery of C.nianzhongi from the Tataertag Formation and C.gui from the Kangshan Formation provide direct evidence on the specific level for the correlation between these two formations,which further supports the Silurian fish-bearing red beds in northwest Zhejiang belonging to the Silurian Lower Red Beds(LRBs)rather than the Upper Red Beds(URBs).Additionally,as the first record of the Changxingaspis in Tarim Basin,it extends the paleogeographical distribution of this genus from the South China Block to the Tarim Block,providing new evidence to support faunal exchanges between these two blocks and the hypothesis of a united Tarim-South China Block during the early Silurian. 展开更多
关键词 vertebrate paleontology Galeaspids Changxingaspis new taxon Tataertag Formation Kangshan Formation Aeronian-Telychian TARIM zhejiang
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Exploring the Spatiotemporal Changes and Driving Forces of Ecosystem Services of Zhejiang Coasts,China,Under Sustainable Development Goals
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作者 ZHANG Shu SUN Chao +2 位作者 ZHANG Yixin HU Ming SHEN Xingru 《Chinese Geographical Science》 SCIE CSCD 2024年第4期647-661,共15页
Ecosystem services(ESs)refer to the continuous provisioning of ecosystem goods and services that benefit human beings.Over recent decades,rapid urbanization has exerted significant pressure on coastal ecosystems,resul... Ecosystem services(ESs)refer to the continuous provisioning of ecosystem goods and services that benefit human beings.Over recent decades,rapid urbanization has exerted significant pressure on coastal ecosystems,resulting in biodiversity and habitat loss,environmental pollution,and the depletion of natural resources.In response to these environmental challenges,the Sustainable Development Goals(SDGs)were proposed.Given the pressing need to address these issues,understanding the changes in ESs under the SDGs is crucial for formulating specific ecological strategies.In this study,we first analyzed land use and cover change in the Zhejiang coasts of China during 2000–2020.Then,we investigated the spatiotemporal configuration of ESs by integrating carbon storage(CS),soil retention(SR),habitat quality(HQ)and water yield(WY)using the Integrated Valuation of Ecosystem Services and Tradeoffs(InVEST)model.The driving mechanisms of ESs,which varied by space and time,were also explored using the Geo-detector method.The results revealed that,over the past two decades:1)the Zhejiang coasts have experienced a significant increase of 2783.72 km^(2) in built-up land areas and a continuous decrease in farmland areas due to rapid urbanization;2)owing to higher precipitation,extensive vegetation cover,and reduced anthropogenic disturbances,forests emerge as a crucial land use type for maintaining ecosystem services such as HQ,CS,WY,and SR;3)ESs have generally declined across the entire Zhejiang coasts,with a significant decrease observed in the northern areas and an increase in the southern areas spatially;4)the expansion of built-up land areas emerged as the primary factor affecting ecosystem services,while the vegetation factor has been increasingly significant and is expected to become predominant in the near future.Our study provides insights of understanding of ecosystem service theory and emphasizing the importance of preserving biodiversity for long-term sustainable development,and valuable scientific references to support the ecological management decision-making for local governments. 展开更多
关键词 ecosystem services(ESs) Integrated Valuation of Ecosystem Services and Tradeoffs(InVEST)model Geo-detector land use and cover change(LUCC) zhejiang coasts China
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浅谈CAPL语言在LIN总线测试中的应用
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作者 姬楠 陆昊楠 +3 位作者 高茹 王晰聪 赵跃 谷原野 《汽车电器》 2024年第9期56-57,60,共3页
文章首先对LIN总线的发展历史和车载应用场景进行简单介绍,然后对LIN总线的测试环境进行详细描述,并结合实际案例,对CAPL语言在LIN总线测试中的应用进行重点阐述。分析表明,CAPL语言可实现对LIN总线测试内容的全覆盖,即使搭配普通电源,... 文章首先对LIN总线的发展历史和车载应用场景进行简单介绍,然后对LIN总线的测试环境进行详细描述,并结合实际案例,对CAPL语言在LIN总线测试中的应用进行重点阐述。分析表明,CAPL语言可实现对LIN总线测试内容的全覆盖,即使搭配普通电源,也可实现对测试时间和测试动作的精确控制,有利于减少测试误差,提高测试效率。 展开更多
关键词 CAPL lin总线 车载网络
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基于LIN总线控制的迈腾B8L近光灯故障诊断方法研究
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作者 李楚欣 黄华 《拖拉机与农用运输车》 2024年第6期31-34,共4页
介绍了迈腾B8L近光灯网络传输架构和功能部件,分析了近光灯信号触发电路、主回路工作电路和网络指示电路,并结合其灯光控制电路,从诊断仪诊断、电压测试、波形分析等方面介绍了检测、诊断迈腾B8灯光系统故障诊断方法。最后以迈腾B8L近... 介绍了迈腾B8L近光灯网络传输架构和功能部件,分析了近光灯信号触发电路、主回路工作电路和网络指示电路,并结合其灯光控制电路,从诊断仪诊断、电压测试、波形分析等方面介绍了检测、诊断迈腾B8灯光系统故障诊断方法。最后以迈腾B8L近灯光异常点亮为案例点,通过检查、分析、推理,排除LIN总线控制近光灯故障,为高效、精准锁定同类电控类故障,提供了借鉴和参考。 展开更多
关键词 lin总线 应急模式 控制单元 故障诊断
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The Spatial-Temporal Characteristics of Meteorological Disasters in the Southwest Region of Zhejiang Province during 1953-2022
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作者 Qi Zhang Yifan Wang +3 位作者 Zhidan Zhu Hongxia Shi Wenhao Yang Shujie Yuan 《Journal of Geoscience and Environment Protection》 2024年第3期16-27,共12页
Meteorological disasters are some of the most serious and costly natural disasters, which have larger effects on economic and social activity. Liuchun Lake is an ecotourism area in the southwest region of Zhejiang pro... Meteorological disasters are some of the most serious and costly natural disasters, which have larger effects on economic and social activity. Liuchun Lake is an ecotourism area in the southwest region of Zhejiang province, where also has experienced meteorological disasters including rainstorm and cold wave. Understanding the temporal-spatial characteristics of meteorological disasters is important for the local tourism and economic development. Based on the daily temperature and precipitation from 18 meteorological stations in the southwest of Zhejiang province during 1953-2022 and some statistical approaches, the temporal and spatial characteristics of meteorological disasters (Freezing, Rainstorm, Cold wave) are analyzed. The results indicate that 1) Rainstorm occurred frequently around the Liuchun lake, the frequency was about 8 times/a, it can also reach about 3 times/a in the other region. Freezing and cold wave (including strong cold wave and extremely cold wave) had the same spatial distribution as rainstorm, however, except for Liuchun lake, they occurred less than one time in the other regions;2) The trend of rainstorm had larger spatial difference, it increased in all the study area, but it increased more significantly around the study area than around Liuchun lake. Freezing was on the downtrend in the whole region, with 93.3% of the stations passed the 95% significant level. Cold wave also showed a declined trend, but it was insignificantly at most of the stations, only 33% of the stations passed the 90% significant level. Compared with cold wave, strong cold wave and extremely strong cold wave had weaker decline in all the regions. In general, from 1953 to 2022 rainstorm showed an increasing trend, it was the main meteorological disaster in the study area, cold wave displayed a decreasing trend, but it still occurred about 2 - 3 times/a in most regions. 展开更多
关键词 Southwest of zhejiang Province RAINSTORM Cold Wave Spatial Distribution Trend Analysis
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Analysis of the Impact of Technical Trade Barriers on the Export of Toys-Based on Analysis of Zhejiang,China
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作者 LIU Ningning CHEN Xiaoxi 《Chinese Business Review》 2024年第1期37-46,共10页
Technical barriers to trade,as a non-tariff barrier,have an increasing impact on the international market.As a major province in toy exports,Zhejiang Province’s toy export trade is therefore greatly hindered.This art... Technical barriers to trade,as a non-tariff barrier,have an increasing impact on the international market.As a major province in toy exports,Zhejiang Province’s toy export trade is therefore greatly hindered.This article aims to explore the impact of technical trade barriers on toy exports in Zhejiang Province and analyze countermeasures.Through literature review and some empirical analysis,it is found that technical trade barriers have greatly promoted the development of Zhejiang’s toy industry;however,they have also subjected it to impacts and pressures such as cost and technology.In response to the impact of technical trade barriers on toy exports,this article proposes corresponding countermeasures to avoid such barriers:firstly,establish a quality management system that complies with export product regulations to ensure that the products produced can meet international requirements and standards;secondly,reduce dependence on overseas markets;then enhance one’s own technology,and strengthen product innovation,in order to enhance the international competitiveness of the product and oneself;in addition,we should also explore diversified markets and expand the sales channels and coverage of our products. 展开更多
关键词 technical trade barriers zhejiang Province export of toys countermeasure analysis
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Other Cities’Distribution Institutions for Publishing Books in Modern Chinese Libraries:Taking Huating Bookstore,the Shanghai General Distribution Office of Modern Zhejiang Library,as an Example
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作者 HE Dan WANG Xirong 《Cultural and Religious Studies》 2024年第3期123-132,共10页
Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zh... Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one. 展开更多
关键词 modern Chinese libraries zhejiang Library Huating Bookstore Yu Heqin book publishing and distribution
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FPX.BerLIN 狂妄的资本
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作者 明诚 《电子竞技》 2024年第4期84-86,共3页
3月2日,在VCT CN联赛启点赛的第四张地图亚海悬城上,彼时FPX以14比15的比分稍稍落后于EDG,全场的压力给到了FPX的队长兼指挥BerLIN身上,他要完成1打3的残局才能帮队伍拖入加时。然而最终奇迹没能上演,FPX以1比3输给了EDG。
关键词 联赛 残局 lin
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基于Lin-GA的磁性示踪粒子六自由度检测技术
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作者 朱霈 郭天太 +2 位作者 刘璐 许新科 孔明 《中国测试》 CAS 北大核心 2024年第3期169-175,共7页
针对基于永磁体和磁传感器阵列的磁定位系统中定位算法存在执行速度慢、定位精度低等问题,提出基于线性算法和遗传算法(linear algorithm-genetic algorithms, Lin-GA)的混合搜索算法。利用自制定位装置中的三轴隧道磁阻(TMR)传感器阵... 针对基于永磁体和磁传感器阵列的磁定位系统中定位算法存在执行速度慢、定位精度低等问题,提出基于线性算法和遗传算法(linear algorithm-genetic algorithms, Lin-GA)的混合搜索算法。利用自制定位装置中的三轴隧道磁阻(TMR)传感器阵列采集磁性示踪粒子在空间产生的弱磁信号,首先通过线性算法利用磁场绝对向量信息进行粗定位,再利用遗传算法对示踪粒子的六自由度参数进行并行寻优,最终实现对磁性示踪粒子三维位置和三维方向的空间信息反演。实验结果表明,基于Lin-GA的混合搜索算法具有更高的定位精度和更短的执行时间,平均定位误差为(0.55±0.10) mm,平均定向误差为(0.68±0.30)°,平均执行时间为(30.49±4.89) s。 展开更多
关键词 磁定位 空间信息反演 lin-GA算法 TMR传感器
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Research on the Internal Talent Training Path and Performance Evaluation Mechanism of New R&D Institutions in Zhejiang Province
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作者 Xiangjiang Li 《Proceedings of Business and Economic Studies》 2024年第2期100-105,共6页
New research and development(R&D)institutions are an important part of the national innovation system,playing an important role in promoting the transformation of scientific and technological achievements.In recen... New research and development(R&D)institutions are an important part of the national innovation system,playing an important role in promoting the transformation of scientific and technological achievements.In recent years,new R&D institutions have gradually become the driving force of innovation-driven development in China.Taking new R&D institutions in Zhejiang Province as the research object,this paper studies the internal talent training path and performance evaluation mechanism of new R&D institutions in Zhejiang Province by using the literature research method,comparison method,case verification method,and other methods.The investigation results show that there are problems such as lack of material and spiritual support and neglect of the absorption of local talents in the internal talent training,and there are problems such as unclear standards,insufficient data,and opaque processes in the performance evaluation mechanism,which greatly affect the establishment and improvement of the performance evaluation mechanism.Given the above problems,this paper puts forward a forward-looking,oriented,flexible,and compatible talent training path and performance evaluation mechanism,hoping to optimize the effective internal talent training path of new R&D institutions,improve the evaluation performance,and promote healthy development of new R&D institutions in Zhejiang Province. 展开更多
关键词 INNOVATION New R&D institutions zhejiang Province Talent cultivation Performance evaluation mechanism
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极其震撼的视觉和听觉体验--LINN&Vivid Audio旗舰新品发布会回顾
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《视听前线》 2024年第4期62-63,共2页
3月16日(周六)上午,泽森音响在广州东方宾馆8楼东方厅举行了LINN&VividAudio新品发布会,LINN CEO Gilad Tiefenbrun和VIVID Audio总设计师LaurenceDickie亲临现场,为到场媒体和发烧友介绍了此次发布会的两款重磅产品:LINNKlimax Sol... 3月16日(周六)上午,泽森音响在广州东方宾馆8楼东方厅举行了LINN&VividAudio新品发布会,LINN CEO Gilad Tiefenbrun和VIVID Audio总设计师LaurenceDickie亲临现场,为到场媒体和发烧友介绍了此次发布会的两款重磅产品:LINNKlimax Solo800旗舰单声道后级、Vivid Audio全新旗舰音箱MoyaM1。 展开更多
关键词 AUDIO 新品发布会 广州东方宾馆 听觉体验 音箱 lin
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RNA结合蛋白Lin28A差异表达可调控牙周膜干细胞的成骨分化 被引量:1
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作者 何琴 卜艳 +3 位作者 林光磊 罗晶 雍敏 黄永清 《中国组织工程研究》 CAS 北大核心 2023年第33期5283-5291,共9页
背景:Lin28A能影响细胞活动的多个方面,包括胚胎干细胞的自我更新、体细胞的重编程、个体的生长发育、组织代谢及致癌作用等,而它对牙周膜干细胞成骨分化的影响国内外均没有深入研究,其影响机制更未见报道。目的:探索Lin28A对牙周膜干... 背景:Lin28A能影响细胞活动的多个方面,包括胚胎干细胞的自我更新、体细胞的重编程、个体的生长发育、组织代谢及致癌作用等,而它对牙周膜干细胞成骨分化的影响国内外均没有深入研究,其影响机制更未见报道。目的:探索Lin28A对牙周膜干细胞成骨分化能力的影响。方法:经典酶消化法分离、培养人牙周膜干细胞,qRT-PCR检测Lin28A在该类细胞中的分布情况及其在成骨诱导不同时间的差异性表达;构建Lin28A过表达及干扰表达的慢病毒载体转染至牙周膜干细胞,荧光显微镜观察转染效果;qRT-PCR验证慢病毒转染后Lin28A的表达差异,并进一步从正反两个方面检测成骨诱导培养过程中成骨基因碱性磷酸酶、Runt相关转录因子2和骨桥蛋白的表达量以及碱性磷酸酶染色和茜素红染色,Western blot检测Runt相关转录因子2的蛋白表达水平;应用生物信息学技术预测和双荧光素酶结合实验验证与Lin28A有关的let-7家族成员之间的相关关系,并通过成骨诱导培养差异表达Lin28A的牙周膜干细胞,测定细胞成骨分化过程中表达变化最明显的miRNA。结果与结论:(1)Lin28A富集在牙周膜干细胞的细胞核中,且在成骨诱导不同时间后呈表达上升的趋势;(2)与空白对照组比较,在过表达Lin28A的牙周膜干细胞模型中,碱性磷酸酶染色和茜素红染色相较空白对照组明显深染,成骨基因碱性磷酸酶、Runt相关转录因子2及骨桥蛋白的表达量分别增加3.3倍、5.1倍及2.2倍(P<0.01);干扰Lin28A表达的细胞模型中,碱性磷酸酶染色和茜素红染色相较对照组染色减少,成骨基因碱性磷酸酶、Runt相关转录因子2及骨桥蛋白的表达量分别下降19%,61%及10%(P<0.01);(3)成骨相关转录因子Runt相关转录因子2的蛋白表达水平与mRNA的差异趋势一致,过表达组呈上升趋势,干扰组呈下降趋势;(4)生物信息学预测出与Lin28A相关的let-7家族成员有8个,分别为let-7a、let-7b、let-7c、let-7d、let-7e、let-7f、let-7g和let-7i,经正常培养和成骨诱导培养筛选发现let-7a和let-7c随Lin28A的表达差异而发生明显的变化,以let-7a的表达趋势最显著;双荧光素酶结合实验也证实let-7a与Lin28A的3’-UTR直接结合,参与影响牙周膜干细胞的成骨分化过程;(5)结果提示:Lin28A参与调控牙周膜干细胞成骨分化,呈正性相关,即过表达Lin28A使牙周膜干细胞成骨能力增强,干扰Lin28A表达后牙周膜干细胞成骨能力减弱;牙周膜干细胞成骨分化受到Lin28A的调控影响。 展开更多
关键词 牙周膜干细胞 成骨分化 lin28A 慢病毒载体 转染 基因干扰 let-7a
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LINC01980在宫颈癌中的表达及调控LIN28B表达促进宫颈癌生长的作用机制
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作者 王春梅 吴德慧 +2 位作者 康燕 魏顺英 马登云 《中国免疫学杂志》 CAS CSCD 北大核心 2023年第2期369-374,共6页
目的:探讨长链非编码RNA01980(LINC01980)在宫颈癌中的表达及其调控LIN28B促进宫颈癌生长的作用机制。方法:生物信息学工具iBio Tools V5.0(http://www.chrisapp.xyz:3838/R/AnnoE2/)预测宫颈癌差异表达基因,catRAPID数据库(http://serv... 目的:探讨长链非编码RNA01980(LINC01980)在宫颈癌中的表达及其调控LIN28B促进宫颈癌生长的作用机制。方法:生物信息学工具iBio Tools V5.0(http://www.chrisapp.xyz:3838/R/AnnoE2/)预测宫颈癌差异表达基因,catRAPID数据库(http://service.tartaglialab.com/page/catrapid_group)在线预测相关RNA结合蛋白。选取2020年1月至2021年5月青海红十字医院收治的病理学诊断为宫颈癌的患者60例作为宫颈癌组,选取同期健康体检者(宫颈未发生病变)60例作为健康组,qRTPCR检测两组研究对象血清LINC01980水平;体外培养宫颈癌细胞Hela,转染并分为Lnc-NC组、si-LINC01980组、si-LINC01980+LIN28B-NC组和si-LINC01980+LIN28B组,对照组仅添加新鲜培养液。RNA pull-down和RNA免疫共沉淀试验检测LINC01980与LIN28B相互作用;qRT-PCR检测Hela细胞LINC01980、LIN28B mRNA表达;CCK-8检测细胞活力;流式细胞术检测细胞周期和凋亡;Western blot检测细胞周期蛋白D1(CyclinD1)、周期蛋白依赖性激酶4(CDK4)、半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达。结果:生物信息学工具iBio Tools V5.0预测结果表明,LINC01980在宫颈癌组织中表达上调;与健康组比较,宫颈癌组血清LINC01980 mRNA表达显著升高(P<0.05);生物信息学网站(http://service.tartaglialab.com/page/catrapid_group)预测结果表明,LINC 01980 RNA序列与LIN28存在结合位点,LINC01980与LIN28B存在相互作用;与对照组比较,si-LINC01980组Hela细胞LINC01980、LIN28B mRNA表达、细胞活力、S期、G2/M期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著降低,细胞凋亡率、G0/G1期细胞百分比、蛋白caspase-3表达显著升高(P<0.05);与si-LINC01980组比较,si-LINC01980+LIN28B组Hela细胞活力、S期、G2/M期期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著升高,细胞凋亡率、G0/G1期细胞百分比、caspase-3蛋白表达显著降低(P<0.05)。结论:LINC01980可能通过调控LIN28B影响宫颈癌细胞增殖和凋亡,参与宫颈癌发生发展,可能是宫颈癌治疗的潜在靶点。 展开更多
关键词 长链非编码RNA 01980 lin28B 宫颈癌 表达 生长
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Analysis of thermohaline and current distribution characteristics of Zhejiang and Fujian waters in summer 被引量:4
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作者 DU Panjun ZHANG Hui +2 位作者 XIAO Wenjun KANG Xing GUAN Qinle 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2011年第5期71-83,共13页
This paper analyzed the distribution of thermohaline and circulation characteristics of Zhejiang and Fujian waters,based on the cross-sectional thermohaline data and on current data (up to 30 d duration) at fixed-po... This paper analyzed the distribution of thermohaline and circulation characteristics of Zhejiang and Fujian waters,based on the cross-sectional thermohaline data and on current data (up to 30 d duration) at fixed-point moorings,collected in the summer of 2006.We also performed low-pass filtering and spectrum analysis on the mooring submersible buoy data.Based on that analysis,we discussed the characteristics of low frequency currents and time-variations in these waters.The main conclusions are as follows.(1) There is a low salinity pinnate area near the Hangzhou Bay in summer,and outside the low salinity area,an obvious salinity front is present from surface to bottom near 123 E.There is also a temperature front below the surface at a corresponding position.(2) Bottom water of the Taiwan Warm Current comes from the subsurface of Kuroshio.(3) The direction of low frequency current at fixed anchor stations is N-NE or S,which mainly depends on the interaction of control currents in this waters.(4) Significant spectral peaks at all mooring stations are typically semidiurnal and diurnal tides.Semidiurnal tidal waves are the main ones in these waters,and have more energy closer to the shore.(5) Significant energy spectral peaks of middle period (3 to 8 d) of currents are responses to weather frequency.(6) Significant energy spectral peaks of long periods at the surface or bottom are probably responses to seasonal wind or bottom friction,while,the long period peaks of other depths can reflect cyclical changes of interactions between currents.We conclude that the pulsation period of the Taiwan Warm Current in these waters is 10-17 d. 展开更多
关键词 zhejiang and Fujian waters thermohaline distribution low-frequency current spectrum analysis Taiwan Warm Current Kuroshio subsurface water
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RNA结合蛋白LIN28对糖尿病肾病的保护机制研究 被引量:1
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作者 王艳丽 李雁 +6 位作者 叶芳 孙燕 侯俊霞 程洁萍 艾比班 李金峰 张新菊 《局解手术学杂志》 2023年第12期1048-1053,共6页
目的探索RNA结合蛋白LIN28对糖尿病肾病(DN)的保护机制。方法在HEK 293T细胞中通过腺病毒介导过表达或敲低LIN28。通过RNA-Seq转录组测序和生物信息学分析获得过表达LIN28后显著改变的基因和功能信号通路。体外过表达LIN28实验分组为Con... 目的探索RNA结合蛋白LIN28对糖尿病肾病(DN)的保护机制。方法在HEK 293T细胞中通过腺病毒介导过表达或敲低LIN28。通过RNA-Seq转录组测序和生物信息学分析获得过表达LIN28后显著改变的基因和功能信号通路。体外过表达LIN28实验分组为Control组、Treatment组、Adv-LIN28-OE+D-核糖组和Adv-LIN28-NC+D-核糖组;敲低LIN28实验分组为Control组、Treatment组、Adv-shRNA LIN28+D-核糖组和Adv-shRNA NT+D-核糖组。通过ELISA法测定上述各组细胞上清液人晚期糖基化终产物(AGEs)的水平。通过Western blot法测定上述各组细胞内RAGE、NF-κB和MMP-2的表达水平。结果RNA-Seq转录组测序和GO分析及KEGG分析结果显示,过表达LIN28导致HEK 293T细胞内AGE-RAGE信号通路显著下调(P<0.05)。ELISA结果显示,与Control组相比,Treatment组细胞产生大量AGEs(P<0.05);与Treatment组相比,Adv-LIN28-OE+D-核糖组细胞AGEs水平明显降低(P<0.05),Adv-shRNA LIN28+D-核糖组细胞AGEs水平显著升高(P<0.05)。Western blot结果显示,与Control组相比,Treatment组细胞内RAGE、NF-κB和MMP-2的表达水平显著升高(P<0.05);与Treatment组相比,Adv-LIN28-OE+D-核糖组细胞内RAGE、NF-κB和MMP-2的表达水平显著降低(P<0.05),Adv-shRNA LIN28+D-核糖组细胞内RAGE、NF-κB和MMP-2的表达水平则显著升高(P<0.05)。结论在体外细胞水平上通过腺病毒过表达LIN28,能够导致数千种基因发生显著性差异表达,特别是能够抑制在DN疾病进展中十分关键的糖尿病-并发症相关AGE-RAGE信号通路,发挥保护DN的作用。 展开更多
关键词 糖尿病肾病 lin28 腺病毒 AGE-RAGE信号通路 NF-ΚB MMP-2
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Lin28B和C-myc蛋白在喉鳞癌中的表达及其与临床病理特征和预后的相关性 被引量:1
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作者 廖非非 曹雨鑫 +2 位作者 黄美响 初令 王天生 《中南大学学报(医学版)》 CAS CSCD 北大核心 2023年第2期165-171,共7页
目的:喉鳞癌是头颈部常见恶性肿瘤。将原癌基因与抑癌基因作为研究的突破口从而筛选恶性肿瘤治疗的靶基因是如今肿瘤研究焦点之一。寻找喉鳞癌治疗及预后相关的靶基因分子至关重要。本研究旨在检测Lin28B和C-myc蛋白在喉鳞癌及其对应癌... 目的:喉鳞癌是头颈部常见恶性肿瘤。将原癌基因与抑癌基因作为研究的突破口从而筛选恶性肿瘤治疗的靶基因是如今肿瘤研究焦点之一。寻找喉鳞癌治疗及预后相关的靶基因分子至关重要。本研究旨在检测Lin28B和C-myc蛋白在喉鳞癌及其对应癌旁组织中的表达,初步探讨其与喉鳞癌临床病理特征及预后的关系。方法:采用免疫组织化学法检测102例喉鳞癌患者的癌组织及其对应的90例癌旁组织中Lin28B与C-myc蛋白的表达情况,分析Lin28B与C-myc蛋白在喉鳞癌中表达的相关性及其表达水平与喉鳞癌临床病理特征之间的关系。采用Kaplan-Meier法分析喉鳞癌患者术后生存率与Lin28B和C-myc蛋白表达水平之间的关系。结果:与癌旁组织相比,Lin28B、Cmyc蛋白的表达水平在喉鳞癌组织中均显著升高(均P<0.05)。且二者在喉鳞癌中表达存在正相关关系(r=0.476,P<0.05)。Lin28B蛋白的表达与喉鳞癌患者年龄、淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。C-myc蛋白的表达与喉鳞癌患者淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。生存分析显示高表达Lin28B(P=0.001)或C-myc蛋白(P<0.001)的患者术后生存率较低,差异有统计学意义。结论:Lin28B及C-myc蛋白在喉鳞癌组织中高表达且呈正相关,两者均与患者的淋巴结转移情况、临床分期、肿瘤大小、病理分化程度及预后相关,提示Lin28B和C-myc可能都参与喉鳞癌的发生、发展。 展开更多
关键词 lin28B C-MYC 喉鳞癌 临床病理特征 预后
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Clinical Engineering Benchmarking Comparison Between Zhejiang Province and American Hospitals 被引量:1
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作者 Binseng Wang Kun Zheng Jing-yi Feng 《中国医疗设备》 2016年第7期1-5,共5页
Clinical engineering(CE) has evolved rapidly over the last 25 years in China. Among the 34 provincial-level administrative units within China, the Zhejiang Province is one of the most advanced in terms of healthcare t... Clinical engineering(CE) has evolved rapidly over the last 25 years in China. Among the 34 provincial-level administrative units within China, the Zhejiang Province is one of the most advanced in terms of healthcare technology maintenance and management. In order to determine Zhejiang's current stage of development and opportunities for further improvement, a comparison of the performance of its CE departments was made against hospitals in the USA. Data were collected from 21 Zhejiang hospitals and compared to those from 270 acute-care hospitals in USA collected by Truven Health Analytics. The benchmarking comparison was made in three categories: operational, financial, and productivity. Within the operational category, the following metrics were compared: equipment inventory size/operating beds, annual repairs/inventory size, and annual scheduled maintenance/inventory size. Within the Financial category, the following metrics were compared: total CE expense/operating beds and total CE expense/total hospital expense. Within the Productivity category, the following metrics were compared: total CE full-time equivalent(FTE)/inventory size and total CE FTE/total hospital expense. These comparisons showed that:(1) While the equipment inventory in Zhejiang tends to be much smaller than USA for hospitals of comparable amount of operating beds, the numbers of repairs and scheduled maintenance per inventory size are similar;(2) The total CE expense/total hospital expense ratio is around 1% in both Zhejiang and USA; however, the total CE expense/operating beds and total CE expense/cost of equipment inventory are significantly lower in Zhejiang than USA;(3) The FTE amount in Zhejiang is significantly higher than in USA relative to both inventory size and total hospital operating expense, but significantly lower relative to the number of operating beds. The fact that repairs and scheduled maintenance are similar in Zhejiang and USA shows that CE leaders are managing equipment in comparable manner. Most of the differences found in the comparisons were traced to a few factors. First, the average length of stay in China is substantially higher than USA, which explains why hospitals in Zhejiang tend to have more operating beds but fewer pieces of equipment. Second, labor cost is significantly lower in China than USA, thus allowing Zhejiang hospitals to employ more workers than their American counterparts. Third, there is significantly difference in the cost of living between China and USA; Finally, being public entities Chinese hospitals are managed and operated in a different manner than American hospitals, which are mostly private, albeit nonprofit organizations. Nonetheless, it is interesting to note that hospitals in both areas spend roughly 1% of their total expenditure for CE. The results suggest that CE in Zhejiang is comparable to USA in terms of managerial excellence but there could be some room for improvement in financial management and productivity. 展开更多
关键词 ClinICAL ENGINEERING performance BENCHMARKING zhejiang PROVINCE hospitals AMERICAN hospitals
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