Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress

The plausibility of human exposure to particulate matter(PM)has witnessed an increase within the last several years.PM of different sizes has been discovered in the atmosphere given the role of dust transport in weather and climate composition.As a regulator,the lung epithelium orchestrates the innate response to local damage.Herein,we developed a lung epithelium-ona-chip platform consisting of easily moldable polydimethylsiloxane layers along with a thin,flexible,and transparent ionic liquid-based poly(hydroxyethyl)methacrylate gel membrane.The epithelium was formed through the culture of human lung epithelial cells(Calu-3)on this membrane.The mechanical stress at the air–liquid interface during inhalation/exhalation was recapitulated using an Arduino-based servo motor system,which applied a uniaxial tensile strength from the two sides of the chip with 10%strain and a frequency of 0.2 Hz.Subsequently,the administration of silica nanoparticles(PM0.5)with an average size of 463 nm to the on-chip platform under static,dynamic,and dynamic+mechanical stress(DMS)conditions demonstrated the effect of environmental pollutants on lung epithelium.The viability and release of lactate dehydrogenase were determined along with proinflammatory response through the quantification of tumor necrosis factor-α,which indicated alterations in the epithelium.

TCT联合HPV E6/E7 mRNA检测对子宫颈癌前早期病变诊断价值的meta分析

目的系统评价液基薄层细胞学检查(thinprep cytologic test,TCT)联合人乳头瘤病毒(human papillomavirus,HPV)E6/E7 mRNA检测对子宫颈癌前早期病变的诊断价值。方法计算机检索CNKI、维普数据库(VIP)、万方数据库、中国生物医学文献数据库(CBM)、PubMed、The Cochrane Library、Embase、Science数据库中关于TCT联合HPV E6/E7 mRNA检测诊断子宫颈癌前早期病变的相关文献,检索时限均为建库至2023年1月。由2位评价员按纳入标准和排除标准各自筛选文献、提取资料和评价质量后,应用RevMan 5.3、Stata 15.0、Meta-Disc 1.4统计软件分析TCT联合HPV E6/E7 mRNA检测对子宫颈癌前早期病变的诊断价值,计算诊断敏感性的Spearman相关系数验证阈值效应,进行异质性检验;计算合并敏感性、特异性、阳性似然比和阴性似然比、诊断比值比、SROC曲线下面积。结果共纳入15篇文献,合计6620例患者。Meta分析显示,合并敏感性、特异性、阳性似然比、阴性似然比、诊断比值比均不存在明显异质性(I^(2)=31.6%、14.2%、10.9%、16.7%、17.2%,P=0.1162、0.2947、0.3311、0.2663、0.2610),采用固定效应模型进行统计分析,其结果分别为0.95(95%CI:0.94~0.96)、0.87(95%CI:0.86~0.88)、6.86(95%CI:6.26~7.51)、0.06(95%CI:0.05~0.07)、113.23(95%CI:86.44~148.31)。SROC曲线下面积为0.9414。结论TCT联合HPV E6/E7 mRNA检测对诊断子宫颈癌前早期病变有重要价值。

Amethod for sharing dynamic geometry information in studies on liquid-based detectors

Liquid-based detectors are widely used in particle and nuclear physics experiments.Because fixed method is used to construct the geometry in detector simulations such as Geant4,it is usually difficult to describe the non-uniformity of the liquid in a detector.We propose a method based on geometry description markup language and a tessellated detector description to share the detector geometry information between computational fluid dynamics simulation software and detector simulation software.This method makes it possible to study the impact of a liquid flow and non-uniformity on the key performance of a liquid-based detector,such as the event vertex reconstruction resolution.This will also be helpful in the detector design and performance optimization.

Comparison of smear cytology with liquid-based cytology in pancreatic lesions: A systematic review and meta-analysis

BACKGROUND Endoscopic ultrasonography-guided fine-needle aspiration(EUS-FNA)is a safe and accurate technique to confirm the diagnosis of pancreatic cancers.Recently,numerous studies comparing the diagnostic efficacy of smear cytology(SC)and liquid-based cytology(LBC)for pancreatic lesions yielded mixed results.AIM To compare and identify the better cytology method for EUS-FNA in pancreatic lesions.METHODS A comprehensive search of PubMed,Embase,and Cochrane was undertaken through July 18,2020.The primary endpoint was diagnostic accuracy(sensitivity and specificity).Secondary outcomes included sample adequacy and post procedure complications.In addition,factors affecting diagnostic efficacy were discussed.RESULTS Data on a total of 1121 comparisons from 10 studies met the inclusion criteria.Pooled rates of sensitivity for SC and LBC were 78%(67%-87%)vs 75%(67%-81%),respectively.In any case,both SC and LBC exhibited a high specificity close to 100%.Inadequate samples more often appeared in LBC compared with SC.However,the LBC samples exhibited a better visual field than SC.Very few post procedure complications were observed.CONCLUSION Our data suggested that for EUS-FNA in pancreatic lesions(particularly solid lesions),SC with Rapid On-Site Evaluation represents a superior diagnostic technique.If Rapid On-Site Evaluation is unavailable,LBC may replace smears.The diagnostic accuracy of LBC depends on different LBC techniques.

Diagnostic value of liquid-based cytology and smear cytology in pancreatic endoscopic ultrasound-guided fine needle aspiration: A meta-analysis

BACKGROUND Smear cytology(SC)using endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)is the established and traditional choice for diagnosing pancreatic lesions.Liquid-based cytology(LBC)is a novel alternative cytological method,however,the comparative diagnostic efficacy of LBC remains inconclusive.AIM To examine the diagnostic efficacy of LBC and SC for pancreatic specimens obtained through EUS-FNA via a systematic review and meta-analysis.METHODS A systematic literature search was performed using PubMed,EMBASE,the Cochrane Library,and Web of Science.The numbers of true positives,false positives,true negatives,and false negatives for each cytological test(LBC and CS)were extracted from the included studies.The pooled sensitivity and specificity and the area under the summary receiver operating characteristic curve(AUC)were calculated,and the AUC was compared by Tukey's multiple comparisons test.The quality of the included studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies II tool.RESULTS A total of 1656 patients in eight studies were included.The pooled sensitivity and specificity and the AUC for LBC were 0.76(95%CI:0.72-0.79),1.00(95%CI:0.98-1.00),and 0.9174,respectively,for diagnosing pancreatic lesions.The pooled estimates for SC were as follows:Sensitivity,0.68(95%CI:0.64-0.71);specificity,0.99(95%CI:0.96-100.00);and AUC,0.9714.Similarly,the corresponding values for LBC combined with SC were 0.87(95%CI:0.84-0.90),0.99(95%CI:0.96-1.00),and 0.9894.Tukey’s multiple comparisons test was used to compare the sensitivities and AUCs of the three diagnostic methods;statistically significant differences were found between the three methods,and LBC combined with SC was superior to both LBC(P<0.05)and SC(P<0.05).The pooled sensitivity and AUC did not change significantly in the sensitivity analysis.CONCLUSION LBC may be sensitive than SC in the cytological diagnosis of pancreatic lesions,however,the superior diagnostic performance of their combination emphasizes their integrated usage in the clinical evaluation of pancreatic lesions.

北京某三甲医院健康体检女性HPV感染情况及TCT结果分析

目的探讨北京某三甲医院健康体检女性HPV感染情况及TCT结果分析,为本地区宫颈癌防治提供参考。方法回顾分析2022年7月至2023年9月于中日友好医院进行健康体检的10124例女性HPV及TCT结果。结果健康体检女性10124例中,HPV阳性率为7.34%,不同年龄段间HPV阳性率比较差异具有统计学意义(χ^(2)=29.721,P<0.001)。高危型HPV占比前五位亚型是HPV⁃52、HPV⁃58、HPV⁃51、HPV⁃16和HPV⁃39。TCT结果阳性率为2.49%,≥61岁人群ASC和HSIL占比最高,21~岁人群LSIL组占比最高。随着宫颈病变加重,从ASC到LSIL再到HSIL,HPV的阳性率逐渐升高(51.41%,70.18%,88.89%),不同程度宫颈病变的HPV阳性率比较差异具有统计学意义(χ^(2)=485.453,P<0.001),不同程度宫颈病变之间HPV感染类型比较差异有统计学意义(χ^(2)=18.943,P<0.001)。结论宫颈病变程度加重,HPV感染率升高,应重视本地区适龄妇女宫颈癌筛查。

Comparative Study of Cell Findings by Conventional Smear and Liquid-Based Cytology for Oral Exfoliative Cytology

Background: Liquid-based cytology (LBC) is a method of manufacturing cyto-diagnostic specimens. Improved accuracy is expected from standardizing specimen production and use of this method is rapidly spreading in oral cytology. On the other hand, LBC reportedly requires training to show peculiar cell findings compared to those of conventional smear cytology (CVC). Few studies have compared detailed cell findings for oral CVC and LBC. Objectives: The aim of this study was to compare cytological findings between CVC and LBC using cytomorphological image analysis. Materials and Methods: Cytological specimens were collected from 20 patients (negative for squamous neoplasia in 10, dysplasia in 5, squamous cell carcinoma in 5) and 5 controls of the tongue between January 2017 and December 2018. Two different preparation techniques were investigated cytomorphologically for CVC and LBC (BD CytorichTM). Results: LBC showed significantly higher cell numbers than CVC for all lesions. LBC-to-CVC ratio ranged from 9.52 (hyperkeratosis) to 1.87 (deep cells in oral squamous cell carcinoma (OSCC)). Nuclear area of normal, hyperkeratosis, and inflammation were significantly higher in LBC than those of CVC. Hyperchromasia was significantly more frequent with CVC than with LBC for hyperkeratosis, inflammation, dysplasia and OSCC. There was no significant difference in circularity between CVC and LBC among all lesions. Conclusion: Only one cytomorphological disadvantage was seen with LBC, in the form of decreased hyperchromasia. Further clarification of the advantages and disadvantages of LBC is needed, including management of precision and screening practices.

Vaginal Intraepithelial Neoplasia Detected with Cervical Liquid-Based Cytology: Old Concerns or New Facilities?

Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hospital das Clínicas of Faculty of Medicine, Sao Paulo State University. Materials and Methods: We evaluated LBC samples from women referred to gynecology examination for different reasons (previous abnormal PapTest, follow up of treated cervical lesion, ecc) and women examined for regular screening proposals, and compared with biopsy diagnoses, including the controversial diagnoses of vaginal intraepithelial neoplasia (VAIN). Results: From 1866 patients, 1423 (76.3%) cases were negative and 443 (23.7%) were positive for any cellular alteration. Age of patients ranged from 12 to 86 years. We detected 25 histologically confirmed VAIN (1.3% p = 0.0002 by Fisher’s exact test IC 95% 0.0090 - 0.0198) and 1.1% VAIN (p = 0.0031 by Fisher’s exact test IC 95% 0.0077 - 0.0179). Conclusion: The identification of VAIN in routine is feasible;the professionals involved with cytological examination should be aware of these lesions in Pap test samples.

Study on the Correlation between Human Papillomavirus and Mycoplasma genitalium Combined with TCT Detection

Objective: This study aims to explore the correlation between human papillomavirus (HPV) and Mycoplasma genitalium (CT) combined with TCT detection in cervical cancer screening. Method: A cross-sectional study design was adopted, and a total of 609 women who came to seek medical treatment were recruited as the study subjects. Combination testing was evaluated on cervical cancer screening by testing the women for HPV, CT with TCT detection and analyzing the relationship of cervical lesions with HPV and CT infection. Results: The study results showed that 21.57% of the subjects were infected with both HPV and CT, and 48.42% of the cases had abnormal TCT results at the same time. Further data analysis showed that HPV infection was significantly associated with abnormal TCT outcomes (p < 0.05), suggesting a possible synergistic effect of the two infections in cervical lesions. The combined sensitivity and specificity of HPV, CT and TCT detection were 21.57% and 48.42%, respectively, which were significantly higher than that of single detection. Conclusion: In summary, the results of this study support the importance of combined HPV, CT, and TCT testing in cervical cancer screening, and propose the hypothesis that combined testing may improve screening effectiveness. However, further large sample studies are needed to confirm this conclusion and explore the prospects of combined testing in clinical practice.

基于TCT-PSA模型的锂离子电池健康状态评估

针对传统估计方法估计精度低的问题,本文提出了一种基于时空卷积网络与金字塔分割注意力融合Transformer(TCT-PSA)模型框架的新型健康状态(SOH)估计方法。首先对NASA电池数据集进行预处理,然后从锂离子电池充电阶段提取健康因子(HF),采用Pearson相关系数和灰色关联分析法量化HF与锂离子电池SOH的相关性,并将相关性高的HF输入到TCT-PSA模型中,SOH为模型输出。为了验证模型的有效性,利用TCT-PSA模型估计各组电池容量衰减;使用不同模型估计各组电池的SOH并进行比较,并利用分位数估计,验证TCT-PSA模型的准确性和鲁棒性。实验结果表明,通过对各组电池测试集和训练集容量衰减的平均绝对估计误差的验证,所提模型的误差均在2%以内;所提模型估计各组电池SOH的平均绝对误差(MAE)、平均绝对百分比误差(MAPE)和均方根误差(RMSE)均在0.035以内;在锂离子电池SOH分位数估计中最高精度达到99.82%。

HPV、TCT及阴道镜对宫颈癌筛查的临床效果研究

目的:探讨宫颈癌筛查采用HPV、TCT及阴道镜的效果。方法:选择医院2021年1月至2022年12月收治疑似宫颈病变患者100例,均接受HPV、TCT检测及阴道镜检查,以病理学检查结果为金标准,分析比较HPV、TCT检测及阴道镜单独与联合检查对宫颈病变的检出率,以及HPV、TCT检测单独与联合检查的诊断效能。结果:该100例疑似宫颈病变患者经病理学检查确诊为宫颈病变者58例,包括有低度鳞状上皮内病变者20例、高度鳞状上皮内病变者27例、宫颈癌患者11例。HPV、TCT检测及阴道镜联合检查的检出率100.00%(58/58),分别高于单独HPV、TCT检测与阴道镜检查的检出率,且单独HPV检测与阴道镜检查的检出率分别为90.91%(430/473)、90.91%(430/473),高于单独TCT检测的检出率(P<0.05)。HPV、TCT联合筛查宫颈癌的准确率、敏感度与阴性预测值分别为90.91%(430/473)、90.91%(430/473)、90.91%(430/473),分别高于单独HPV、TCT检测(P<0.05)。结论:在宫颈癌筛查中,联合TCT、HPV检测及阴道镜检查可显著的提升筛查准确率,能够及时的对疾病进行确诊并给予针对性的处理,以改善患者预后。

宫颈HPVE6/E7检测联合计算机辅助诊断TCT及阴道炎检测在宫颈细胞学筛查中的应用价值

目的 分析宫颈人乳头瘤病毒(HPV)E6/E7检测联合计算机辅助诊断宫颈液基细胞学(TCT)及阴道炎检测在宫颈细胞学筛查中的应用价值。方法 采用宫颈HPVE6/E7检测、计算机辅助诊断TCT、阴道炎检测法和阴道炎+TCT+HPVE6/E7检测对2023年2~8月在北京市昌平区中西医结合医院检查的1 586例女性进行宫颈癌筛查,发现181例疑似高级别鳞状上皮内病变(HSIL)病例,对疑似“HSIL”追加病理检查,并以病理检查作为金标准,分析上述筛查4种方法对HSIL的检出率及病理检查结果的一致性,并计算4种筛查方法对HSIL诊断的灵敏度和特异度。结果 181例疑似HSIL患者经病理检查发现共86例HSIL患者。阴道炎检测对HSIL的检出率为16.57%,Kappa值为0.488。计算机辅助诊断TCT检测对HSIL的检出率为24.86%,Kappa值为0.554。宫颈HPVE6/E7检测对HSIL的检出率为19.34%,Kappa值为0.512。阴道炎+TCT+HPVE6/E7检测对HSIL的检出率为38.67%,Kappa值为0.742。阴道炎+TCT+HPVE6/E7检测的AUC值>0.85,预测价值较高,灵敏度、特异度分别为81.40%、84.21%;阴道炎检测、TCT检测、HPVE6/E7检测的AUC值均>0.70,预测价值一般,各检测方法灵敏度、特异度分别为34.88%、52.63%,52.33%、61.05%,40.70%、64.21%。结论 宫颈HPVE6/E7、计算机辅助诊断TCT及阴道炎检测联合筛查可在一定程度上提升宫颈病变的检出率,对宫颈病变诊断具有明显指导意义。

TCT联合HR-HPV检测在宫颈癌及癌前病变早期筛查中的应用价值

目的探讨在宫颈癌及癌前病变早期筛查中选择液基薄层细胞检测(TCT)联合高危型人乳头状瘤病毒(HRHPV)检测的实际价值及有效性。方法选择2000例行宫颈癌及癌前病变早期筛查的患者,研究对象均取自2020年1月-2023年1月。均行TCT、HR-HPV检查。诊断金标准以病理结果为依据,对比TCT、HR-HPV单项及联合检测与病理结果的一致性。结果病理检查结果显示,2000例患者中,阳性共396例,阴性共1604例。TCT+HR-HPV联合检测的灵敏度94.41%、特异性98.50%及准确率98.80%均明显高于TCT单项检测80.00%、87.83%、86.95%、HR-HPV单项检测82.10%、94.81%、92.40%,具统计学意义(P<0.05)。TCT+HR-HPV检查的阳性、阴性预测值93.93%、98.62%明显高于TCT单项检测45.45%、97.19%和HR-HPV单项检测78.78%、95.76%,误诊率、漏诊率5.58%、1.51%明显低于TCT单项检测20.00%、12.17%和HR-HPV单项检测17.89%、5.18%,具统计学意义(P<0.05)。结论通过TCT+HR-HPV检测进行宫颈癌及癌前病变早期筛查,可显著提升诊断准确率,有助于临床早期诊治,提升诊疗质量,且对患者生存周期具有延长作用。

HPV16/18型阳性且TCT正常患者发生HSIL+风险预测模型的构建

目的探究HPV16/18阳性且薄层细胞学检测(thinprep cytologic test,TCT)正常患者发生宫颈高级别上皮内病变及以上(high-GRADE squamous intraepithelial lesion or worse,HSIL+)病变的风险因素并构建风险预测模型,以期为临床医生提供一种转诊阴道镜及宫颈活检的辅助评估工具。方法收集2021年6月至2023年6月就诊于内蒙古自治区人民医院门诊,HPV16/18型阳性且TCT正常并行阴道镜+宫颈活组织病理学检查的患者,共207例,通过Logistic回归分析筛选独立风险因素,构建预测模型,建立连线图,并评估模型的预测性能、符合度、准确度及临床实用性。结果(1)单因素卡方检验显示,BMI(χ^(2)=12.13,P<0.001)、吸烟(χ^(2)=15.28,P<0.001)、阴道微生态(χ^(2)=10.074,P=0.002)、感染时间(χ^(2)=82.444,P<0.001)、阴道状态(χ^(2)=66.458,P<0.001)为HPV16/18阳性且TCT正常患者发生HSIL+的独立风险因素;(2)风险预测模型的AUC为0.94(95%CI=0.89~0.98);(3)列线图模型进行内部验证后,其一致性指数(C-index)为0.937。结论本研究基于BMI、阴道微生态、吸烟、感染时间及阴道状态等影响因素建立的预测模型预测HPV16/18型阳性且TCT正常患者发生HSIL+具有较好的区分度、准确度及临床实用性,可为临床医生面对HPV16/18型阳性且TCT正常患者是否行阴道镜+宫颈活组织病理学检查提供参考意见。

HPV联合TCT检测在健康体检宫颈病变中的应用分析

目的:探究人乳头瘤病毒(HPV)联合液基波层细胞学(TCT)检测在健康体检宫颈病变中的应用分析。方法:纳入研究对象为在我院健康体检中心行宫颈筛查的患者,经后续检查最终被确诊为宫颈病变的患者70例,研究对象纳入时间段为2021年1月~2023年2月,所有患者均进行了人乳头瘤病毒(HPV)检测、液基波层细胞学(TCT)检测。以病理诊断结果为金标准,比较HPV检测、TCT检测、HPV联合TCT检测的准确率。统计分析所有宫颈病变患者单一检测及联合检测的灵敏度、特异性数据。结果:三种检测结果显示,单一HPV及TCT检测的阳性检出率均低于HPV联合TCT检测,组间比较有差异(P<0.05),同时TCT检测阳性检出率低于HPV检测,组间比较有差异(P<0.05)。HPV检测的灵敏度与特异度分别为68.59%、77.92%,TCT检测的灵敏度与特异性分别为63.22%、73.13%,HPV联合TCT检测的灵敏度与特异度分别为84.06%、93.96%,组间差异有统计学意义(P<0.05)。结论:在健康体检过程中,采用HPV联合TCT检测进行宫颈筛查,可较为准确地判定出体检者是否存在宫颈病变,对早期宫颈病变诊断具有较好的临床价值。

早期宫颈癌筛查中应用HPV,TCT和阴道镜的临床价值分析

早期宫颈癌筛查中应用HPV(人乳头瘤病毒)、TCT(液基薄层细胞学检测)和阴道镜的临床价值分析。方法 选取常州市武进第六人民医院2022年1月至2023年11月期间收治的疑似宫颈癌癌前病变的130例患者作为研究样本,采用TCT、HPV联合阴道镜检查,以阴道镜下宫颈病理活检作为金标准,分析诊断效能。结果 TCT、HPV检测结果分析,CIN3病变患者TCT、HPV检测结果均为阳性概率为66.67%(4/6),随着宫颈活检病理级别的升高,TCT、HPV检测准确率升高,检测诊断应用价值越高。单一TCT、HPV与阴道镜联合检测检测准确率评估,联合检测准确率高于单一TCT检测、单一HPV检测,P<0.05,单一HPV检测准确率高于单一TCT检测,P<0.05。结论 阴道镜和TCT联合HPV检查是广泛用于早期宫颈癌筛查的常见方法,在准确诊断临床疾病方面表现出了极高的可靠性,这种筛查方法的应用价值不可低估。

TCT、HPV及阴道镜联合检查在宫颈癌筛查中的应用价值

探讨在宫颈癌早期筛查中TCT、HPV及阴道镜联合检测的价值。方法 研究对象为于2023年1月-2023年12月于我院体检中心进行体检的女性体检者1200人,所有参检者均进行TCT、HPV联合筛查,发现有400例高度怀疑宫颈病变者进行阴道镜检查,并对其诊断效果进行分析。结果 HPV检测中正常+炎症组阳性率与TCT检测中的LSIL组、HSIL组相比,具有显著差异性,P<0.05;HSIL组与ASCUS组相比,阳性率有较大差异,P<0.05,TCT结果越高时,HPV检测阳性率也随之增加;CINⅡ组、CINⅠ、CINⅢ组对比中,CINⅢ组阳性检测率较高,组间对比P<0.05;同时,TCT联合HPV进行检测时,发现阳性率较高,CINⅡ组、CINⅠ组未出现漏诊情况,结果准确性高。结论 TCT、HPV和阴道镜联合检查可以提供更准确,更全面的宫颈癌筛查结果,从而降低患者的疾病风险,对于改善宫颈癌患者的预后具有重要意义。

HPV分型检测联合TCT及宫颈活检在宫颈癌筛查中的临床价值分析

探讨人乳头瘤病毒(HPV)分型检测联合液基薄层细胞学检查(TCT)、宫颈活检在宫颈癌筛查中的临床价值。方法 选择2020年3月1日-2022年12月31日时间范围内326例疑似宫颈癌妇女,均接受HPV分型检测、TCT检测以及宫颈活检。将病理学检查结果作为各项检测方案的“金标准”,进一步分析HPV分型、TCT以及宫颈活检在宫颈癌的诊断价值。结果 326例疑似宫颈癌妇女中,经病理学确诊宫颈癌6例,CINⅠ38例,CINⅡ40例,CINⅢ22例;在同一级别病变检出率中,HPV分型联合TCT、宫颈活检检出率最高,比较有意义(P<0.05),且在鳞状上皮癌(SCC)、宫颈鳞状上皮内病变Ⅲ级(CINⅢ)检出率达100%;HPV分型联合TCT、宫颈活检在敏感度、特异度、诊断效率、阳性预测值、阴性预测值4个诊断效能方面明显高于HPV分型、TCT、宫颈活检单一检测以及HPV分型联合TCT检测,比较有意义(P<0.05)。结论 HPV分型、TCT及宫颈活检三者联合检测能够提高宫颈癌筛查的诊断效能。

HPV、TCT在早期宫颈癌筛查中的作用

研究在早期宫颈癌筛查中应用HPV和TCT诊断的作用。方法 于我院慢性宫颈炎或疑似早期宫颈癌的患者中随机抽取90例,接受TCT、HPV和病理活检,统计检查结果。结果 联合诊断符合率、敏感度均显著高于HPV和TCT单独诊断(P<0.05),和病理诊断结果无显著差异(P>0.05)。HPV诊断符合率、特异度略高于TCT诊断,但未见显著差异(P>0.05)。结论 在早期宫颈癌筛查中,HPV+TCT联合检查的准确率、敏感度更高,更接近病理诊断结果。因此临床上可积极采取HPV+TCT联合检查进行宫颈癌筛查,支持临床诊疗工作的开展。

TCT联合HPV分型检测在宫颈癌前病变筛查中的应用价值

对宫颈癌前病变筛查中液基薄层细胞检测(Thinprep Cytologic Test, TCT)联合人乳头状瘤病毒(High Permeability to Water Vapour, HPV)分型检测的应用价值进行分析。方法 纳入2021年3月~2024年2月宫颈癌筛查人员768例,均对其实施了TCT检测和合HPV分型检测。对上述两种检测方法检测情况进行分析、汇总和对比。结果 对检测结果进行分析可知,高危型的HPV的阳性率与病理级别呈现出正相关的趋势,其阳性率越高则病理级别越高;而低危型的HPV感染则主要集中在CINⅠ、CINⅡ这两个级级当中。对TCT阳性进行分析可知,其宫颈活检级别越高,HSIL、宫颈癌随之升高。联合检测诊断符合率为94.08%,较HPV分型、TCT检测诊断符合率83.43%、66.27%更高,P<0.05。结论 在对宫颈癌前病变进行筛查时,给予TCT联合HPV分型检测能够显著提高检查的准确率,能够使患者得到更早的诊断,有利于疾病的早期鉴别诊断。