Identification and Transcriptional Regulation of CAMTA Genes in Liriodendron chinense

This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored.Within the L.chinense genome,we identified two CAMTA genes,Lchi09764 and Lchi222536,characterized by four functional domains:CG-1,TIG,ANK repeats,and IQ motifs.Our analyses,including phylogenetic investigations,cis-regulatory element analyses,and chromosomal location studies,aim to elucidate the defining features of CAMTA genes in L.chinense.Applying Weighted Gene Co-Expression Network Analysis(WGCNA),we explored the impact of CAMTA genes on different organs and their regulation under abiotic stress conditions.The identification of significant gene modules and the prediction of promoter binding sites revealed co-expressed genes associated with CAMTA transcription factors.In summary,this study provides initial insights into CAMTA genes in L.chinense,laying the groundwork for future research on their evolution and biological roles.This knowledge enhancement contributes to a better understanding of plant responses to environmental stress—an essential aspect of plant biology.

Genome-wide identification and cold stress-induced expression analysis of the CBF gene family in Liriodendron chinense

Cold-resistance pathways that operate in model plants such as Arabidopsis thaliana and Oryza sativa have been studied extensively.It has been found that CBF genes play an important role in plant cold resistance.Liriodendron chinense,a tree known for its graceful tree shape and widely spread in south China,has weak cold tolerance.However,little is known about its response to cold.To further study the function of L.chinense CBF gene family,we started by characterizing all members of this gene family in the L.chinense genome and their expression profiling.Phylogenetic analysis found that 14 CBF genes in L.chinense are more closely related to their homologues in woody plants and A.thaliana than those in O.sativa.Cis-acting elements and GO analysis showed that some LcCBF genes participated in the biological process of cold stress response.The transcriptomic and RT-qPCR data showed that most of LcCBF genes displayed an initially increasing and subsequently decreasing trend during cold stress course and the expression profile of each member was different.Some LcCBF genes exhibited a different abundance in callus,root,stem and leaf tissues.The structure and expression characteristics of LcCBF genes imply that they may have similar and different functions in response to cold stress conditions.The identification and analysis of LcCBF gene family have laid the foundation for future studies into L.chinense cold stress mechanisms and for the cultivation of cold-resistance cultivars.

Changes in soil organic carbon and aggregate stability following a chronosequence of Liriodendron chinense plantations

The objectives for this study were to determine changes in soil organic carbon(SOC)components and water-stable aggregates for soil profi les from diff erent ages of plantations of Liriodendron chinense and to clarify which organic carbon component is more closely associated with the formation and stability of soil aggregates.Three layers of soil(depths 0–20 cm,20–40 cm,40–60 cm)were collected from young,half-mature and mature stages of L.chinense.SOC,readily oxidizable organic carbon,chemically stable organic carbon and aggregate composition were determined.Intermediate stable organic carbon,the microbial quotient and aggregate stability(mean weight diameter)were calculated.SOC and aggregate stability in the L.chinense plantation did not increase linearly with an increase in L.chinense age;rather,they fi rst decreased,then increased with increasing age of L.chinense.The microbial quotient had a negative eff ect on the level of organic carbon and the stability of aggregates,while chemically stable organic carbon had a positive eff ect,which explained 55.0%and 19.3%of the total variation,respectively(P<0.01).Therefore,more attention should be paid of these two indicators in the future.

Research on the Effects of Light Intensity and Seeding Density on the Seed Germination and Seedling Growth of Liriodendron chinense

It provides a theoretical basis for the artificial promotion of the reproduction and regeneration of Liriodendron chinense through exploring the effects of light intensity and seeding density on the seed germination and seedling growth of this rare plant. Controlled experiments were conducted by setting different seeding densities and shading intensities to observe and detect the seed germination and seedling growth under different experimental conditions. The data were analyzed by SPSS and the model was fitted to calculate the seed germination rate, seedling emergence rate and seedling growth parameters of Liriodendron chinense, which were used to analyze the effects of seeding density and light intensity on the seed germination and seedling growth of Liriodendron chinense. Light has a significant impact on the seed germination and seedling growth of Liriodendron chinense (P P > 0.05). High density promotes the seedling height (the seedling height showed a rising trend with the increase of seeding density, reaching the highest at 500 seeds/m2. “3-stitch + 300 seeds/m2” combination is an appropriate condition for Liriodendron chinense reproduction since it can realize higher germination rate and better seedling growth potential. The seedling height and basal diameter growth curve of Liriodendron chinense fits well to the Logistic equation (R2 ≥ 0.977). Light has a significant impact on the seed germination and seedling growth of Liriodendron chinense, and appropriate shading treatment can increase the seed germination rate and seedling emergence rate of Liriodendron chinense. Seeding density has little influence on the seed germination rate of Liriodendron chinense, but high density will promote seedling height.