The direct detection of clenbuterol（CL） in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond（BDD） electrode is reported. The pyrrole-DNA modified BDD electrode has a strong ...The direct detection of clenbuterol（CL） in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond（BDD） electrode is reported. The pyrrole-DNA modified BDD electrode has a strong electrocatalytic effect on the redox reaction of CL. One oxidization and two reduction peaks of CL appear at 340. 2, 299. 8 and 166. 6 mV（ versus SCE）, respectively. The pyrrole polymer alone cannot electrocatalyze the above reaction at a BDD electrode ; the electrocatalytic effect of a BDD electrode modified with DNA membrane is unsufficient for the analytical detection of CL; the replacement of boron-doped diamond by glass carbon makes the electrocatalytic reaction impossible ; the redox process is pH dependent. The influences of various experimental parameters on the pyrrole-DNA modified BDD electrode were investigated. A sensitive cyclic vohammetric response for CL was obtained in a linear range from 3.4 × 10^-6 to 5 × 10^ -4 mol/L with a detection limit of 8.5 × 10^-7 mol/L. A mean recovery of 102. 7% of CL in the pig liver sample solution and a reproducibility of 3.2% were obtained.展开更多
建立了分子印迹固相萃取/高效液相色谱法测定猪肝中盐酸克伦特罗残留量的方法。以ASB C18色谱柱（250 mm×4.60 mm,5μm）分离,流动相为甲醇-水（45∶55,p H 3.0）,紫外检测波长243 nm,进样量10μL,流速0.6 m L/min,柱温30℃。分子...建立了分子印迹固相萃取/高效液相色谱法测定猪肝中盐酸克伦特罗残留量的方法。以ASB C18色谱柱（250 mm×4.60 mm,5μm）分离,流动相为甲醇-水（45∶55,p H 3.0）,紫外检测波长243 nm,进样量10μL,流速0.6 m L/min,柱温30℃。分子印迹固相萃取条件为：上样溶剂为双蒸水,上样流速为0.6 m L/min;洗脱剂为1%三氟乙酸的甲醇溶液。上述优化条件下,该方法在0.432~4.32μg/m L范围内线性关系良好,相关系数（r）为0.999 0。加标回收率为84.7%~92.0%,相对标准偏差（RSD）为1.5%~1.8%。方法的准确度和精密度满足残留分析的要求。展开更多
为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,...为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,电泳分离时间小于6 m in;在最佳实验条件下,克伦特罗和沙丁胺醇的检出限分别为0.4和0.3μg/kg。方法灵敏度高、特异性强,测定结果准确可靠,可用于畜产品中克伦特罗和沙丁胺醇残留的确证性检测。展开更多
基金Supported by the National Natural Science Foundation of China(Nos. 20435010, 20375012, 20205005 and 20475014).
文摘The direct detection of clenbuterol（CL） in pig liver without any extraction separation at a pyrrole-DNA modified boron-doped diamond（BDD） electrode is reported. The pyrrole-DNA modified BDD electrode has a strong electrocatalytic effect on the redox reaction of CL. One oxidization and two reduction peaks of CL appear at 340. 2, 299. 8 and 166. 6 mV（ versus SCE）, respectively. The pyrrole polymer alone cannot electrocatalyze the above reaction at a BDD electrode ; the electrocatalytic effect of a BDD electrode modified with DNA membrane is unsufficient for the analytical detection of CL; the replacement of boron-doped diamond by glass carbon makes the electrocatalytic reaction impossible ; the redox process is pH dependent. The influences of various experimental parameters on the pyrrole-DNA modified BDD electrode were investigated. A sensitive cyclic vohammetric response for CL was obtained in a linear range from 3.4 × 10^-6 to 5 × 10^ -4 mol/L with a detection limit of 8.5 × 10^-7 mol/L. A mean recovery of 102. 7% of CL in the pig liver sample solution and a reproducibility of 3.2% were obtained.
文摘建立了分子印迹固相萃取/高效液相色谱法测定猪肝中盐酸克伦特罗残留量的方法。以ASB C18色谱柱（250 mm×4.60 mm,5μm）分离,流动相为甲醇-水（45∶55,p H 3.0）,紫外检测波长243 nm,进样量10μL,流速0.6 m L/min,柱温30℃。分子印迹固相萃取条件为：上样溶剂为双蒸水,上样流速为0.6 m L/min;洗脱剂为1%三氟乙酸的甲醇溶液。上述优化条件下,该方法在0.432~4.32μg/m L范围内线性关系良好,相关系数（r）为0.999 0。加标回收率为84.7%~92.0%,相对标准偏差（RSD）为1.5%~1.8%。方法的准确度和精密度满足残留分析的要求。
文摘为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,电泳分离时间小于6 m in;在最佳实验条件下,克伦特罗和沙丁胺醇的检出限分别为0.4和0.3μg/kg。方法灵敏度高、特异性强,测定结果准确可靠,可用于畜产品中克伦特罗和沙丁胺醇残留的确证性检测。
