OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver. METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selec...OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver. METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selection technique with the recombinant antigen on solid surface. The positive clones were demonstrated by ELISA and their DNA sequences were also determined. RESULTS: The DNA sequence data showed that the antibody gene is composed of 768bp. In addition, a specific combination capacity with recombinant Echinococcus granulosus antigen B (r-EgB) was demonstrated by ELISA. CONCLUSION: The obtained gene engineering antibody against r-EgB may have potential implications in immunological treatment and drug targeting delivery.展开更多
<strong>Aim:</strong> This article aimed to investigate the analysis of various methods for eliminating the residual liver cavity after laparoscopic echinococcectomy in patients. <strong>Methods:<...<strong>Aim:</strong> This article aimed to investigate the analysis of various methods for eliminating the residual liver cavity after laparoscopic echinococcectomy in patients. <strong>Methods:</strong> The authors used the following methods of eliminating the residual cavity: omentoplasty (36.8%), abdominalization (30%), drainage of the residual cavity (26.2%), and a combination of omentoplasty with drainage of the residual cavity (7%). <strong>Results:</strong> In the postoperative period, complications occurred in 2 (3.5%) cases in the form of bile leakage from the residual liver cavity. <strong>Conclusions:</strong> It is recommended to use abdominalization and omentoplasty when choosing a method for eliminating the residual liver cavity.展开更多
Thirteen years ago, liver alveolar echinococcosis (LAE) in the Northwest of China was reported by the first author. In 1993, 70 cases of LAE were diagnosed at Lanzhou Hydatid Research Laboratory of Lanzhou Medical Col...Thirteen years ago, liver alveolar echinococcosis (LAE) in the Northwest of China was reported by the first author. In 1993, 70 cases of LAE were diagnosed at Lanzhou Hydatid Research Laboratory of Lanzhou Medical College. In order to sum up the present and previous experiences, clinical and pathological analyses of the 70 cases were made and the factors related to the prognosis of the patients were discussed.展开更多
AIM: To describe patient characteristics and the results of surgical treatment for hepatic hydatidosis (HH) in the Hospital Hernán Henríquez Aravena from December 2001 to March 2005. METHODS: Subjects older ...AIM: To describe patient characteristics and the results of surgical treatment for hepatic hydatidosis (HH) in the Hospital Hernán Henríquez Aravena from December 2001 to March 2005. METHODS: Subjects older than 16 years with suspected HH were included based on the clinical picture and radiological studies. Variables examined included clinical and laboratory aspects, outcome, features of the parasite and surgical aspects. Descriptive statistics were used, calculating central tendency, dispersion and extreme tendency. RESULTS: The series was comprised of 122 patients, with an average age of 44 ± 16.9 years. The most frequently used surgical technique was subtotal cys-tectomy in 90% of the patients, followed by hepatic resection (hepatectomy, segmentectomy and subsegmentectomy) in 5%. In 2%, a combination of subtotal cystectomy and segmentectomy was performed. In addition, 28% of the series presented complications in the postoperative period and mortality was 2%. CONCLUSION: The most frequently used surgical technique for HH was subtotal cystectomy and the morbidity and mortality rates in this Chilean series are comparable to other national and international series.展开更多
基金The project was supported by a grant from the National Natural Science Fundation of China (No. 39860078) and Xinjiang Natural Science Fundation China (No. 200221101).
文摘OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver. METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selection technique with the recombinant antigen on solid surface. The positive clones were demonstrated by ELISA and their DNA sequences were also determined. RESULTS: The DNA sequence data showed that the antibody gene is composed of 768bp. In addition, a specific combination capacity with recombinant Echinococcus granulosus antigen B (r-EgB) was demonstrated by ELISA. CONCLUSION: The obtained gene engineering antibody against r-EgB may have potential implications in immunological treatment and drug targeting delivery.
文摘<strong>Aim:</strong> This article aimed to investigate the analysis of various methods for eliminating the residual liver cavity after laparoscopic echinococcectomy in patients. <strong>Methods:</strong> The authors used the following methods of eliminating the residual cavity: omentoplasty (36.8%), abdominalization (30%), drainage of the residual cavity (26.2%), and a combination of omentoplasty with drainage of the residual cavity (7%). <strong>Results:</strong> In the postoperative period, complications occurred in 2 (3.5%) cases in the form of bile leakage from the residual liver cavity. <strong>Conclusions:</strong> It is recommended to use abdominalization and omentoplasty when choosing a method for eliminating the residual liver cavity.
文摘Thirteen years ago, liver alveolar echinococcosis (LAE) in the Northwest of China was reported by the first author. In 1993, 70 cases of LAE were diagnosed at Lanzhou Hydatid Research Laboratory of Lanzhou Medical College. In order to sum up the present and previous experiences, clinical and pathological analyses of the 70 cases were made and the factors related to the prognosis of the patients were discussed.
文摘AIM: To describe patient characteristics and the results of surgical treatment for hepatic hydatidosis (HH) in the Hospital Hernán Henríquez Aravena from December 2001 to March 2005. METHODS: Subjects older than 16 years with suspected HH were included based on the clinical picture and radiological studies. Variables examined included clinical and laboratory aspects, outcome, features of the parasite and surgical aspects. Descriptive statistics were used, calculating central tendency, dispersion and extreme tendency. RESULTS: The series was comprised of 122 patients, with an average age of 44 ± 16.9 years. The most frequently used surgical technique was subtotal cys-tectomy in 90% of the patients, followed by hepatic resection (hepatectomy, segmentectomy and subsegmentectomy) in 5%. In 2%, a combination of subtotal cystectomy and segmentectomy was performed. In addition, 28% of the series presented complications in the postoperative period and mortality was 2%. CONCLUSION: The most frequently used surgical technique for HH was subtotal cystectomy and the morbidity and mortality rates in this Chilean series are comparable to other national and international series.
