Biological basis of “depression with liver-qi stagnation and spleen deficiency syndrome”: A digital gene expression profiling study

Objective:To investigate the biological basis of“depression with liver-qi stagnation and spleen deficiency syndrome”.Methods:A digital gene expression profiling method was conducted to explore global changes in the mRNA transcriptome in a rat model of depression with liver-qi stagnation and spleen deficiency syndrome.Real-time quantitative polymerase chain reaction(q-PCR)was performed to verify the five genes most interest based on the Kyoto Encyclopedia of Genes and Genome(KEGG)analysis.Sini San,which disperses stagnated liver qi and strengthens the spleen,was administered to the model rats to observe whether it could reverse these genetic changes in the liver.Results:Forty-six differentially expressed genes were identified.Three of the five genes of most interestdHnf4a,Hnf4g and Cyp1a1dbased on KEGG analysis,were confirmed by realtime q-PCR.Sini San reduced the gene expression changes of Hnf4a,Hnf4g and Cyp1a1 in the rat model.Conclusions:Hnf4a,Hnf4g and Cyp1a1 are involved in“depression with liver-qi stagnation and spleen deficiency syndrome”.These findings indicate that depressed rats with liver-qi stagnation and spleen deficiency syndrome are at risk of liver diseases.Furthermore,our results will inform exploration of the etiology of depression and help in the development of effective therapeutic strategies.

四逆散对肝郁证模型大鼠胃肠组织细胞形态及胃肠激素的影响

目的:研究四逆散对肝郁证模型大鼠胃肠组织细胞形态及胃肠激素的影响。方法:将大鼠分为3组:空白对照组.肝郁模型组,四逆散治疗组。通过运用模具加水浴方法制备肝气郁结证候动物模型。观察各组大鼠的症状表现、胃肠组织形态学变化、胃肠超微结构变化,测定大鼠的智力、胃肠激素。结果:四逆散能使模型大鼠活动及体重均增加,学习记忆能力增强,使大鼠胃肠的病理改变得以很好的恢复,能提高血清中胃泌素、血浆中胃动素和血管活性肠肽的含量,降低血浆中生长抑素的含量。结论:四逆散通过保护胃肠黏膜,调节胃肠激素,使肝胆疏泄及脾胃升降恢复正常,气机调畅。

大鼠肝郁证胃肠运动功能障碍动物模型的建立

目的通过运用模具加水浴方法制备肝气郁结证候动物模型。方法将大鼠分为空白对照组、模型组、自复组、反证组4组。运用模具加水浴方法造成大鼠肝郁证胃肠运动功能障碍动物模型。测量各组动物的体重、腹围、自发活动、智力、胃和小肠运动功能变化,观察胃肠组织形态学变化,测定血浆胃动素(MTL)、胃泌素(Gas)、生长抑素(SS)、血管活性肠肽(VIP)指标研究肝郁证大鼠胃肠激素的变化。结果模型组大鼠体重增加减慢,腹围指数变大,自发活动减少,智力下降;胃残留率增加、小肠推进率减慢;Gas、MTL和VIP的含量降低,SS的含量升高。用四逆散治疗后上述指标恢复正常。结论用模具加水浴方法制备肝郁证动物模型接近中医肝气郁结证的发病学因素,其模型的情志改变呈现肝郁证典型的临床特点。

慢性非萎缩性胃炎患者症状与精神状态关系初探

目的分析慢性非萎缩性胃炎患者临床症状与精神状态的关系。方法对胃镜确诊慢性非萎缩性胃炎患者264例,采用抑郁PHQ-9量表、焦虑自评量表SAS及临床症状问卷进行调查分析。结果 264例完成调查的研究对象中,抑郁的检出率为51.9%,焦虑的检出率为19.7%。抑郁较焦虑更明显(P<0.001)。恶心、腹胀、腹泻与PHQ-9得分相关性评价,r值大于0.2,P值<0.001。结论慢性非萎缩性胃炎患者抑郁比焦虑明显。恶心、腹胀、腹泻与PHQ-9得分正相关更明显一些。