BACKGROUND Lomatogonium rotatum(LR)is traditionally used in Mongolian folk medicine as a hypoglycemic agent,but its evidence-based pharmacological effects and mechanisms of action have not been fully elucidated.AIM To...BACKGROUND Lomatogonium rotatum(LR)is traditionally used in Mongolian folk medicine as a hypoglycemic agent,but its evidence-based pharmacological effects and mechanisms of action have not been fully elucidated.AIM To emphasize the hypoglycemic action mechanism of LR in a type 2 diabetic rat model and examine potential biomarkers to obtain mechanistic understanding regarding serum metabolite modifications.METHODS A high-fat,high-sugar diet and streptozotocin injection-induced type 2 diabetic rat model was established.The chemical composition of the LR was identified by high performance liquid chromatography.LR extract administrated as oral gavage at 0.5 g/kg,2.5 g/kg,and 5 g/kg for 4 wk.Anti-diabetic effects of LR extract were evaluated based on histopathological examination as well as the measurement of blood glucose,insulin,glucagon-like peptide 1(GLP-1),and lipid levels.Serum metabolites were analyzed using an untargeted metabolomics approach.RESULTS According to a chemical analysis,swertiamarin,sweroside,hesperetin,coumarin,1.7-dihydroxy-3,8-dimethoxyl xanthone,and 1-hydroxy-2,3,5 trimethoxanone are the principal active ingredients in LR.An anti-diabetic experiment revealed that the LR treatment significantly increased plasma insulin and GLP-1 levels while effectively lowering blood glucose,total cholesterol,triglycerides,low-density lipoprotein cholesterol,and oral glucose tolerance test compared to the model group.Furthermore,untargeted metabolomic analysis of serum samples detected 236 metabolites,among which 86 were differentially expressed between the model and the LR group.It was also found that LR considerably altered the levels of metabolites such as vitamin B6,mevalonate-5P,Dproline,L-lysine,and taurine,which are involved in the regulation of the vitamin B6 metabolic pathway,selenium amino acid metabolic pathway,pyrimidine metabolic pathway,and arginine and proline metabolic pathways.CONCLUSION These findings indicated that LR may have a hypoglycemic impact and that its role may be related to changes in the serum metabolites and to facilitate the release of insulin and GLP-1,which lower blood glucose and lipid profiles.展开更多
Thirteen xanthones were isolated from L.rotatum,which were established as l,8-dihydroxy-3,4,5-trimethoxyxanthone(1);l-hydroxy-3异,8-trimethoxyxanthone(2);8-hydroxy-l,3?5-trimethoxyxaiithone(3);l-hydroxy-3,4,5-trimetho...Thirteen xanthones were isolated from L.rotatum,which were established as l,8-dihydroxy-3,4,5-trimethoxyxanthone(1);l-hydroxy-3异,8-trimethoxyxanthone(2);8-hydroxy-l,3?5-trimethoxyxaiithone(3);l-hydroxy-3,4,5-trimethoxyxanthone(4);l-hydroxy-3,5,8-trimethoxyxanthone(5);l,4,8-trihydroxy-5,6-dimethoxyxanthone(6);l,3,8-trihydroxy-4,7-dimethoxyxanthone(;7)l,8-dihydroxy-3,7-dimethoxyxanthone(8);l-hydroxy-3,5-dimethoxyxanthone(9);l-hydroxy-2,3,4,5-tetramethoxyxanthone(;10)l-hydroxy-2,3,4,7-tetramethoxyxanthone(11);l,7-dihydroxy-3,8-dimethoxyxanthone(12);rel-l,3,5,8-tetrahydroxy-2,4,6,7-tetrahydroxanthen-9-one(13).Compounds 4,6,7,9,10,11,12 and 13 are reported for the first time from Lomatogonium.Furthermore,the activity of xanthones was assessed.展开更多
Lomatogonium rotatum(L.rotatum)Fries ex Nym,a dry whole grass belonging to the family Gentianaceae,is widely used to treat liver diseases in Mongolian medicine.In Mongolian medicine,L.rotatum Fries ex Nym,also known a...Lomatogonium rotatum(L.rotatum)Fries ex Nym,a dry whole grass belonging to the family Gentianaceae,is widely used to treat liver diseases in Mongolian medicine.In Mongolian medicine,L.rotatum Fries ex Nym,also known as Digeda,is a rare medicinal herb with low yield and widespread clinical use.Currently,it is included in over 25 traditional Mongolian medicine prescriptions that help reduce heat,dispel xieri,enhance stomach function,and heal wounds.Recent studies have shown that L.rotatum Fries ex Nym contains a variety of metabolites,including flavonoids,xanthone compounds,terpenoids,organic acids,steroids,and alkaloids.In addition,its anti-hepatitis B,anti-inflammatory,anti-acute liver injury,and anti-obesity effects have been proven by pharmacological studies.In this review,we summarize the ecological resources,traditional pharmacodynamics,chemical constituents,and pharmacological actions of L.rotatum Fries ex Nym,with an aim to provide a theoretical basis for future applied research and new product development.展开更多
Objective:To study the chemical constituents from traditional Chinese(Mongolian)medicine,Lomatogonium carinthiacum and Halenia corniculate.Methods:The chemical constituents were isolated and purified by silicagel colu...Objective:To study the chemical constituents from traditional Chinese(Mongolian)medicine,Lomatogonium carinthiacum and Halenia corniculate.Methods:The chemical constituents were isolated and purified by silicagel column,Sephadex LH-20,ODS and high performance liquid chromategramphy.The structures were identified by NMR and MS analysis technics.Results:Twelve compounds were isolated and identified as isovitexin(1),Luteolin-5-O-β-D-glucoside(2),Isosaponarin(3),Luteolin-7-O-β-D-glucoside(4,7),1,4,8-Trimethoxy-xanthone-6-O-β-D-glucoronyl-(1→6)O-β-Dglucoside(5),friginoside D(6),1-hydroxy-2,3,5-trimethoxyxanthone(8),1-hydroxy-2,3,4,5-tet ramethoxyxanthone(9),1-hydroxy-2,3,4,7-tetramethoxyxanthone(10),1-hydroxy-2,3,4,5,7-pentame thoxyxanthone(11)and usnic acid(12).Conclusion:Compounds 6 and 12 are obtained from L.carinthiacum and H.corniculate for the first time.展开更多
AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral...AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.展开更多
目的研究肋柱花(Lomatogonium rotatum(L.)Fries ex Nym.)的化学成分。方法用硅胶、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质及光谱数据确定化合物的结构。结果分离得到10个化合物,分别鉴定为1,8-二羟基-3,4,5-三甲氧基呫...目的研究肋柱花(Lomatogonium rotatum(L.)Fries ex Nym.)的化学成分。方法用硅胶、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质及光谱数据确定化合物的结构。结果分离得到10个化合物,分别鉴定为1,8-二羟基-3,4,5-三甲氧基呫吨酮(1,8-dihydroxy-3,4,5-trimethoxyxanthone,1)、1-羟基-3,7,8-三甲氧基呫吨酮(1-hydroxy-3,7,8-trimethoxyxanthone,2)、8-羟基-1,3,5-三甲氧基呫吨酮(8-hydroxy-1,3,5-trimethoxyxanthone,3)、1-羟基-3,5,8-三甲氧基呫吨酮(1-hydroxy-3,5,8-trimethoxyxanthone,4)、红白金内酯(erythrocentaurin,5)、木犀草素(luteolin,6)、芹菜素(apigenin,7)、5,7,3′,4′,5′-五羟基黄酮(5,7,3′,4′,5′-pentahydroxyflaveone,8)、槲皮素(quercetin,9)、齐墩果酸(oleanolic acid,10)。结论化合物1~4、8、9为首次从肋柱花属中分离得到。展开更多
基金Supported by the National Natural Science Foundation of China,No.82260773 and 81803845Central Government Guided Local Scientific and Technological Development Project,No.2021ZY0015Science and Technology Young Talents Development Project of Inner Mongolia Autonomous Region,No.NJYT22048.
文摘BACKGROUND Lomatogonium rotatum(LR)is traditionally used in Mongolian folk medicine as a hypoglycemic agent,but its evidence-based pharmacological effects and mechanisms of action have not been fully elucidated.AIM To emphasize the hypoglycemic action mechanism of LR in a type 2 diabetic rat model and examine potential biomarkers to obtain mechanistic understanding regarding serum metabolite modifications.METHODS A high-fat,high-sugar diet and streptozotocin injection-induced type 2 diabetic rat model was established.The chemical composition of the LR was identified by high performance liquid chromatography.LR extract administrated as oral gavage at 0.5 g/kg,2.5 g/kg,and 5 g/kg for 4 wk.Anti-diabetic effects of LR extract were evaluated based on histopathological examination as well as the measurement of blood glucose,insulin,glucagon-like peptide 1(GLP-1),and lipid levels.Serum metabolites were analyzed using an untargeted metabolomics approach.RESULTS According to a chemical analysis,swertiamarin,sweroside,hesperetin,coumarin,1.7-dihydroxy-3,8-dimethoxyl xanthone,and 1-hydroxy-2,3,5 trimethoxanone are the principal active ingredients in LR.An anti-diabetic experiment revealed that the LR treatment significantly increased plasma insulin and GLP-1 levels while effectively lowering blood glucose,total cholesterol,triglycerides,low-density lipoprotein cholesterol,and oral glucose tolerance test compared to the model group.Furthermore,untargeted metabolomic analysis of serum samples detected 236 metabolites,among which 86 were differentially expressed between the model and the LR group.It was also found that LR considerably altered the levels of metabolites such as vitamin B6,mevalonate-5P,Dproline,L-lysine,and taurine,which are involved in the regulation of the vitamin B6 metabolic pathway,selenium amino acid metabolic pathway,pyrimidine metabolic pathway,and arginine and proline metabolic pathways.CONCLUSION These findings indicated that LR may have a hypoglycemic impact and that its role may be related to changes in the serum metabolites and to facilitate the release of insulin and GLP-1,which lower blood glucose and lipid profiles.
基金We thank the fourth national survey of traditional Chinese medicine resources(2018016)for funding this research.
文摘Thirteen xanthones were isolated from L.rotatum,which were established as l,8-dihydroxy-3,4,5-trimethoxyxanthone(1);l-hydroxy-3异,8-trimethoxyxanthone(2);8-hydroxy-l,3?5-trimethoxyxaiithone(3);l-hydroxy-3,4,5-trimethoxyxanthone(4);l-hydroxy-3,5,8-trimethoxyxanthone(5);l,4,8-trihydroxy-5,6-dimethoxyxanthone(6);l,3,8-trihydroxy-4,7-dimethoxyxanthone(;7)l,8-dihydroxy-3,7-dimethoxyxanthone(8);l-hydroxy-3,5-dimethoxyxanthone(9);l-hydroxy-2,3,4,5-tetramethoxyxanthone(;10)l-hydroxy-2,3,4,7-tetramethoxyxanthone(11);l,7-dihydroxy-3,8-dimethoxyxanthone(12);rel-l,3,5,8-tetrahydroxy-2,4,6,7-tetrahydroxanthen-9-one(13).Compounds 4,6,7,9,10,11,12 and 13 are reported for the first time from Lomatogonium.Furthermore,the activity of xanthones was assessed.
基金National Natural Science Foundation of China,No.81803845Natural Science Foundation of Inner Mongolia Autonomous Region,No.2018MS08040+1 种基金Construction Project of"Inner Mongolia Autonomous Region Mongolian Medicine and Food Source Protection and Utilization Innovation Team",No.190301Graduate Research Project of Inner Mongolia Minzu University,No.NMDBS1901.
文摘Lomatogonium rotatum(L.rotatum)Fries ex Nym,a dry whole grass belonging to the family Gentianaceae,is widely used to treat liver diseases in Mongolian medicine.In Mongolian medicine,L.rotatum Fries ex Nym,also known as Digeda,is a rare medicinal herb with low yield and widespread clinical use.Currently,it is included in over 25 traditional Mongolian medicine prescriptions that help reduce heat,dispel xieri,enhance stomach function,and heal wounds.Recent studies have shown that L.rotatum Fries ex Nym contains a variety of metabolites,including flavonoids,xanthone compounds,terpenoids,organic acids,steroids,and alkaloids.In addition,its anti-hepatitis B,anti-inflammatory,anti-acute liver injury,and anti-obesity effects have been proven by pharmacological studies.In this review,we summarize the ecological resources,traditional pharmacodynamics,chemical constituents,and pharmacological actions of L.rotatum Fries ex Nym,with an aim to provide a theoretical basis for future applied research and new product development.
基金supported by the Inner Mongolia Autonomous Region undergraduate colleges and universities directly undergraduates in 2019 "Double First class" construction special fund Mongolian Medicine Research and Innovation Team Construction Project (No.190302)2021 Grassland Talents (No.CYYC21003)Open Project of NMPA Key Laboratory f Quality Control of Traditional Chinese Medicine (Mongolian Medicine)(No.MDK2021073)
文摘Objective:To study the chemical constituents from traditional Chinese(Mongolian)medicine,Lomatogonium carinthiacum and Halenia corniculate.Methods:The chemical constituents were isolated and purified by silicagel column,Sephadex LH-20,ODS and high performance liquid chromategramphy.The structures were identified by NMR and MS analysis technics.Results:Twelve compounds were isolated and identified as isovitexin(1),Luteolin-5-O-β-D-glucoside(2),Isosaponarin(3),Luteolin-7-O-β-D-glucoside(4,7),1,4,8-Trimethoxy-xanthone-6-O-β-D-glucoronyl-(1→6)O-β-Dglucoside(5),friginoside D(6),1-hydroxy-2,3,5-trimethoxyxanthone(8),1-hydroxy-2,3,4,5-tet ramethoxyxanthone(9),1-hydroxy-2,3,4,7-tetramethoxyxanthone(10),1-hydroxy-2,3,4,5,7-pentame thoxyxanthone(11)and usnic acid(12).Conclusion:Compounds 6 and 12 are obtained from L.carinthiacum and H.corniculate for the first time.
基金supported by the National Natural Science Foundation of China(No.81360673)
文摘AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.