This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique ...This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique LRWVT,and the water vapor from the South China Sea and the western Pacific can affect the Sichuan Basin, Northwest China and other Chinese regions far from the tropical sea through this way. From August to October, the precipitation of the region east of the Plateau is closely linked both in the intra-annual and inter-annual variations, and the LRWVT from the South China Sea and the western Pacific is an important connection mechanism. The large-scale circulation background of the LRWVT impacting the precipitation of the region east of the Plateau is as follows: At high levels,the South Asian High is generally stronger than normal and significantly enhances with its northward advance and eastward extension over the region east of the Plateau. At mid-level, a broad low pressure trough is over Lake Balkhash and its surroundings, and the Western Pacific Subtropical High(WPSH) is northward and westward located, and the western part of Sichuan Basin and the eastern part of Northwest China are located in the west and northwest edge of WPSH.展开更多
BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified...BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3＇ untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3＇ untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.展开更多
Nicotinamide adenine dinucleotide （NAD） biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate （NA） via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-ci...Nicotinamide adenine dinucleotide （NAD） biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate （NA） via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA （NA methyl ester） has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase （designated AtNaMT1） capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase （MES2） predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress （salt, abscisic acid, and mannitol） treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.展开更多
Numerical modelling of coastal morphology is a complex and sometimes unrewarding exercise and often not yielding tangible results. Typically, the underlying drivers of morphology are not properly accounted for in nume...Numerical modelling of coastal morphology is a complex and sometimes unrewarding exercise and often not yielding tangible results. Typically, the underlying drivers of morphology are not properly accounted for in numerical models. Such inaccuracies combined with a paucity of validation data create a difficulty for coastal planners/engineers who are required to interpret such morphological models to develop coastal management strategies. This study develops an approach to long term morphological modelling of a barrier beach system that includes the findings of over 10 years of coastal monitoring on a dynamic coastal system. The novel approach to predicting the long term evolution of the area combines a mix of short term hydrodynamic monitoring and long term morphological modelling to predict future changes in a breached barrier system. A coupled wave, wind, hydrodynamic and sediment transport numerical model was used to predict the coastal evolution in the dynamic barrier beach system of Inner Dingle Bay, Co. Kerry, Ireland. The modelling approach utilizes the schematisation of inputs to reflect observed trends. The approach is subject to two stages of validation both quantitative and qualitative. The study highlights the importance of considering all the parameters responsible for driving coastal evolution and the necessity to have long term monitoring results for trend based validation.展开更多
基金Major Program of the National Natural Science Foundation of China(41290255)National Natural Science Foundation of China(41305082)Fundamental Research Funds of Chengdu Institute of Plateau Meteorology,CMA
文摘This paper attempts to reveal a long-distance-relayed water vapor transport(LRWVT) east of Tibetan Plateau and its impacts. The results show that from August to October, east of Tibetan Plateau, there exists a unique LRWVT,and the water vapor from the South China Sea and the western Pacific can affect the Sichuan Basin, Northwest China and other Chinese regions far from the tropical sea through this way. From August to October, the precipitation of the region east of the Plateau is closely linked both in the intra-annual and inter-annual variations, and the LRWVT from the South China Sea and the western Pacific is an important connection mechanism. The large-scale circulation background of the LRWVT impacting the precipitation of the region east of the Plateau is as follows: At high levels,the South Asian High is generally stronger than normal and significantly enhances with its northward advance and eastward extension over the region east of the Plateau. At mid-level, a broad low pressure trough is over Lake Balkhash and its surroundings, and the Western Pacific Subtropical High(WPSH) is northward and westward located, and the western part of Sichuan Basin and the eastern part of Northwest China are located in the west and northwest edge of WPSH.
基金supported by the United States Departmentof Agriculture NRI-CGP award no.2008-02806National Science Foundation-Plant Genome Research Program award no.0820659
文摘BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3＇ untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3＇ untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.
文摘Nicotinamide adenine dinucleotide （NAD） biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate （NA） via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA （NA methyl ester） has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase （designated AtNaMT1） capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase （MES2） predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress （salt, abscisic acid, and mannitol） treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.
文摘Numerical modelling of coastal morphology is a complex and sometimes unrewarding exercise and often not yielding tangible results. Typically, the underlying drivers of morphology are not properly accounted for in numerical models. Such inaccuracies combined with a paucity of validation data create a difficulty for coastal planners/engineers who are required to interpret such morphological models to develop coastal management strategies. This study develops an approach to long term morphological modelling of a barrier beach system that includes the findings of over 10 years of coastal monitoring on a dynamic coastal system. The novel approach to predicting the long term evolution of the area combines a mix of short term hydrodynamic monitoring and long term morphological modelling to predict future changes in a breached barrier system. A coupled wave, wind, hydrodynamic and sediment transport numerical model was used to predict the coastal evolution in the dynamic barrier beach system of Inner Dingle Bay, Co. Kerry, Ireland. The modelling approach utilizes the schematisation of inputs to reflect observed trends. The approach is subject to two stages of validation both quantitative and qualitative. The study highlights the importance of considering all the parameters responsible for driving coastal evolution and the necessity to have long term monitoring results for trend based validation.
