Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich pla...Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.展开更多
目的探讨抗生素骨水泥联合Ilizarov技术个性化治疗长骨感染性骨缺损的方法和临床疗效。方法自2011年6月至2017年1月,我院应用Ilizarov技术个性化治疗股骨、胫骨、肱骨骨折术后感染性骨缺损21例,男14例,女7例;年龄18～65岁,平均37岁。其...目的探讨抗生素骨水泥联合Ilizarov技术个性化治疗长骨感染性骨缺损的方法和临床疗效。方法自2011年6月至2017年1月,我院应用Ilizarov技术个性化治疗股骨、胫骨、肱骨骨折术后感染性骨缺损21例,男14例,女7例;年龄18～65岁,平均37岁。其中胫骨骨缺损12例,清创后骨缺损范围平均7.2cm;股骨骨缺损7例,清创后骨缺损范围平均8.5cm;肱骨骨缺损2例,清创后骨缺损范围平均6cm。手术分两期进行:一期彻底清创,植入含敏感抗生素骨水泥,采用单边或环形外固定架固定,预留截骨延长的外固定架长度,控制感染;二期感染消灭后,根据Ilizarov技术进行截骨延长。结果所有患者术后随访12～24个月,平均18.5个月。外固定架固定时间9～21个月,平均17.2个月。外固定架指数(external fixation index,EFI)平均为2.38个月/cm。采用伊里扎洛夫方法研究与应用学会(association for the study and application of the method of Ilizarov,ASAMI)评价标准评价,骨性结果:优13例,良6例,中2例,优良率为90.5%;功能结果:优11例,良7例,中3例,优良率为85.7%。结论抗生素骨水泥联合Ilizarov技术可有效控制感染,纠正患肢畸形,重建肢体的功能,是治疗长骨感染性骨缺损的有效方法。展开更多
文摘Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.
文摘目的探讨抗生素骨水泥联合Ilizarov技术个性化治疗长骨感染性骨缺损的方法和临床疗效。方法自2011年6月至2017年1月,我院应用Ilizarov技术个性化治疗股骨、胫骨、肱骨骨折术后感染性骨缺损21例,男14例,女7例;年龄18～65岁,平均37岁。其中胫骨骨缺损12例,清创后骨缺损范围平均7.2cm;股骨骨缺损7例,清创后骨缺损范围平均8.5cm;肱骨骨缺损2例,清创后骨缺损范围平均6cm。手术分两期进行:一期彻底清创,植入含敏感抗生素骨水泥,采用单边或环形外固定架固定,预留截骨延长的外固定架长度,控制感染;二期感染消灭后,根据Ilizarov技术进行截骨延长。结果所有患者术后随访12～24个月,平均18.5个月。外固定架固定时间9～21个月,平均17.2个月。外固定架指数(external fixation index,EFI)平均为2.38个月/cm。采用伊里扎洛夫方法研究与应用学会(association for the study and application of the method of Ilizarov,ASAMI)评价标准评价,骨性结果:优13例,良6例,中2例,优良率为90.5%;功能结果:优11例,良7例,中3例,优良率为85.7%。结论抗生素骨水泥联合Ilizarov技术可有效控制感染,纠正患肢畸形,重建肢体的功能,是治疗长骨感染性骨缺损的有效方法。
