Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology...Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology represented by the Internet of Things,cloud computing,mobile networks and big data,and urbanization.How to effectively achieve the long-term preservation of massive,heterogeneous,and multi-source digital electronic records in smart cities is a key issue thatmust be solved.Digital continuity can ensure the accessibility,integrity and availability of information.The quality management of electronic record,like the quality management of product,will run through every phase of the urban lifecycle.Based on data quality management,this paper constructs digital continuity of smart city electronic records.Furthermore,thework in this paper ensures the authenticity,integrity,availability and timeliness of electronic documents by quality management of electronic record.This paper elaborates on the overall technical architecture of electronic record,as well as the various technical means needed to protect its four characteristics.展开更多
Spirulina is an edible algae and has a wide range of pharmaceutical applications in addition to its nutritional value. Isolation and identification of several Spirulina species were conducted in the western part of Me...Spirulina is an edible algae and has a wide range of pharmaceutical applications in addition to its nutritional value. Isolation and identification of several Spirulina species were conducted in the western part of Mexico especially in the state of Jalisco. The purification strategy consisted of five optimized processing steps: 1) washing and centrifugation, 2) chemical treatment, 3) micromanipulation, 4) serial dilution, and 5) plating. Four species were isolated from different locations and two out of these four species were identified taxonomically up to the species level: Spirulina subsalsa and S. major. For short term conservation (30 days), the strains were maintained in liquid and solid agar medium at 10?C and 4?C. For medium term (few months), they were preserved in solid medium under a dried condition as agar flakes and for long term, cryopreservation was employed by using 5% and 10% DMSO, glycerol and methanol as osmoprotectants in liquid nitrogen. For short term preservation nearly 90% liquid and 100% agar recovered strains were viable after one month at both temperatures. In the case of the agar flakes, cells were viable after three months of conservation at room temperature. Cryopreservation did not give any suitable results after three months of conservation. Variable and two-step improved cryopreservation processes are now in progress for conservation.展开更多
基金the NSFC (Nos. 61772280, 62072249)the AIrecognition scoring system of weather map (No. SYCX202011)+1 种基金the national training programsof innovation and entrepreneurship for undergraduates (Nos. 201910300123Y, 202010300200)the PAPD fund from NUIST. Jinyue Xia is the corresponding author.
文摘Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology represented by the Internet of Things,cloud computing,mobile networks and big data,and urbanization.How to effectively achieve the long-term preservation of massive,heterogeneous,and multi-source digital electronic records in smart cities is a key issue thatmust be solved.Digital continuity can ensure the accessibility,integrity and availability of information.The quality management of electronic record,like the quality management of product,will run through every phase of the urban lifecycle.Based on data quality management,this paper constructs digital continuity of smart city electronic records.Furthermore,thework in this paper ensures the authenticity,integrity,availability and timeliness of electronic documents by quality management of electronic record.This paper elaborates on the overall technical architecture of electronic record,as well as the various technical means needed to protect its four characteristics.
基金We deeply acknowledge Biotecnologia Mexicana de Mi-croalgas,S.A.and Consejo Nacional de Ciencia y Tec-nologia(CONACYT),Mexico for financial support.
文摘Spirulina is an edible algae and has a wide range of pharmaceutical applications in addition to its nutritional value. Isolation and identification of several Spirulina species were conducted in the western part of Mexico especially in the state of Jalisco. The purification strategy consisted of five optimized processing steps: 1) washing and centrifugation, 2) chemical treatment, 3) micromanipulation, 4) serial dilution, and 5) plating. Four species were isolated from different locations and two out of these four species were identified taxonomically up to the species level: Spirulina subsalsa and S. major. For short term conservation (30 days), the strains were maintained in liquid and solid agar medium at 10?C and 4?C. For medium term (few months), they were preserved in solid medium under a dried condition as agar flakes and for long term, cryopreservation was employed by using 5% and 10% DMSO, glycerol and methanol as osmoprotectants in liquid nitrogen. For short term preservation nearly 90% liquid and 100% agar recovered strains were viable after one month at both temperatures. In the case of the agar flakes, cells were viable after three months of conservation at room temperature. Cryopreservation did not give any suitable results after three months of conservation. Variable and two-step improved cryopreservation processes are now in progress for conservation.
