OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloi...OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1(TRPV1).METHODS: Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents. Rat experiments in vivo were used to observe the analgesic effects of TFDB. Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.RESULTS: Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion(DRG) neurons of rats and the half inhibitory concentration was(16.7 ± 1.6) mg/L.TFDB(2-20 mg/kg) showed analgesic activity in the phase Ⅱ of formalin test and(0.02-2 mg per paw)reduced capsaicin-induced licking times of rats. TFDB(20 mg/kg) was fully efficacious on complete Freund's adjuvant(CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects. The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.CONCLUSION: All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.展开更多
目的:探讨595 nm脉冲染料激光与重组人表皮生长因子(rhEGF)治疗面部瘢痕的疗效。方法:随机选取80例面部瘢痕患者。分为对照组(n = 40,595 nm激光治疗)与研究组(n = 40,595 nm激光治疗联合rhEGF),对比两组瘢痕面积、瘢痕愈合情况及评分...目的:探讨595 nm脉冲染料激光与重组人表皮生长因子(rhEGF)治疗面部瘢痕的疗效。方法:随机选取80例面部瘢痕患者。分为对照组(n = 40,595 nm激光治疗)与研究组(n = 40,595 nm激光治疗联合rhEGF),对比两组瘢痕面积、瘢痕愈合情况及评分、满意度。结果:研究组在瘢痕面积、结痂与痂皮脱落时间、创面愈合时间、瘢痕上的评分均显著低于对照组(P Objective: To investigate the efficacy of 595 nm pulsed dye laser combined with recombinant human epidermal growth factor (rhEGF) in the treatment of facial scars. Methods: A total of 80 patients with facial scars were randomly selected and divided into a control group (n = 40, treated with 595 nm laser alone) and a study group (n = 40, treated with 595 nm laser combined with rhEGF). The scar area, scar healing status, scar score, and patient satisfaction were compared between the two groups. Results: The study group showed significantly lower scar area, shorter scab and eschar shedding time, faster wound healing time, and lower scar scores compared to the control group (P < 0.05). Additionally, patient satisfaction was significantly higher in the study group than in the control group (P < 0.05). Conclusion: The combination of 595 nm pulsed dye laser and rhEGF exhibits remarkable efficacy in the treatment of facial scars, promoting wound healing, reducing scar area, and improving facial scar conditions.展开更多
目的:研究黄金射频微针联合注射A型肉毒毒素及外涂重组人表皮生长因子(Recombinant human epidermal growth factor,rhEGF)凝胶治疗痤疮瘢痕的效果。方法:研究者筛选出苏州和常州美莱美容医院2021年7月-2022年7月接诊的面部痤疮愈后形...目的:研究黄金射频微针联合注射A型肉毒毒素及外涂重组人表皮生长因子(Recombinant human epidermal growth factor,rhEGF)凝胶治疗痤疮瘢痕的效果。方法:研究者筛选出苏州和常州美莱美容医院2021年7月-2022年7月接诊的面部痤疮愈后形成瘢痕的患者共63例,并采用随机数字表法划分为研究组(n=31)和对照组(n=32)两小组。对照组选用黄金射频微针联合外涂rhEGF凝胶作为常规治疗,而研究组则在此基础上注射A型肉毒毒素联合治疗。治疗后,比较两组痤疮瘢痕修复的临床疗效、痤疮瘢痕评估量表权重评分、各种不良反应的发生情况。结果:治疗期间对照组不良反应发生率(18.75%)高于研究组(12.9%),但差异无统计学意义(P>0.05)。治疗后,研究组患者治疗后的总有效率为90.32%,高于对照组的65.63%(P<0.05)。研究组患者治疗后的ECCA评分值小于对照组(P<0.05)。结论:A型肉毒毒素注射结合黄金射频微针及rhEGF凝胶治疗痤疮瘢痕有较高的疗效,其能在保证安全性的基础上,改善痤疮后遗症。展开更多
[目的]研究重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)在治疗外伤性鼓膜穿孔过程中的作用。[方法]对门诊就诊的外伤性鼓膜穿孔患者123例患者,随机的分别采用4种不同的治疗方法(即常规治疗方法、贴膜法、应用...[目的]研究重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)在治疗外伤性鼓膜穿孔过程中的作用。[方法]对门诊就诊的外伤性鼓膜穿孔患者123例患者,随机的分别采用4种不同的治疗方法(即常规治疗方法、贴膜法、应用rhEGF喷剂法、贴膜和rhEGF喷剂联合应用法),定期观察鼓膜愈合情况、瘢痕的轻重,记录鼓膜完全愈合的时间,按鼓膜穿孔的大、中、小,分3组进行统计学处理。[结果]对小穿孔4种治疗方法差异无显著性意义(愈合时间,P>0.05),对中等穿孔,传统组、贴膜组与rhEGF组、贴膜+rhEGF组之间分别两两比较差异有显著性意义(P<0.05)。对大穿孔,传统组、贴膜组、贴膜+rhEGF组三者之间两两比较差异均有显著性意义(P<0.05)。[结论]外伤性鼓膜穿孔中的中等穿孔和大穿孔应用rhEGF或rhEGF与贴膜联合应用,可以显著地缩短愈合时间,并减轻鼓膜瘢痕,提高治愈率。展开更多
基金High Level Talents Project of Affiliated Hospital of Youjiang Medical University for Nationalities:Study of Soft-Du'an Capsule's Mechanism and Efficacy of Regulating TRPV1 Pashways in Relieving Oral and Maxillofacial Trigeminal Neuralgia (No. YYFYR20213002)Innovative Group Project of Natural Science Foundation of Hubei Province:Study on the Mechanisms of Pain Signal Transduction and Drug Analgesia (No. 2020CFA025)。
文摘OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1(TRPV1).METHODS: Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents. Rat experiments in vivo were used to observe the analgesic effects of TFDB. Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.RESULTS: Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion(DRG) neurons of rats and the half inhibitory concentration was(16.7 ± 1.6) mg/L.TFDB(2-20 mg/kg) showed analgesic activity in the phase Ⅱ of formalin test and(0.02-2 mg per paw)reduced capsaicin-induced licking times of rats. TFDB(20 mg/kg) was fully efficacious on complete Freund's adjuvant(CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects. The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.CONCLUSION: All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.
文摘目的:探讨595 nm脉冲染料激光与重组人表皮生长因子(rhEGF)治疗面部瘢痕的疗效。方法:随机选取80例面部瘢痕患者。分为对照组(n = 40,595 nm激光治疗)与研究组(n = 40,595 nm激光治疗联合rhEGF),对比两组瘢痕面积、瘢痕愈合情况及评分、满意度。结果:研究组在瘢痕面积、结痂与痂皮脱落时间、创面愈合时间、瘢痕上的评分均显著低于对照组(P Objective: To investigate the efficacy of 595 nm pulsed dye laser combined with recombinant human epidermal growth factor (rhEGF) in the treatment of facial scars. Methods: A total of 80 patients with facial scars were randomly selected and divided into a control group (n = 40, treated with 595 nm laser alone) and a study group (n = 40, treated with 595 nm laser combined with rhEGF). The scar area, scar healing status, scar score, and patient satisfaction were compared between the two groups. Results: The study group showed significantly lower scar area, shorter scab and eschar shedding time, faster wound healing time, and lower scar scores compared to the control group (P < 0.05). Additionally, patient satisfaction was significantly higher in the study group than in the control group (P < 0.05). Conclusion: The combination of 595 nm pulsed dye laser and rhEGF exhibits remarkable efficacy in the treatment of facial scars, promoting wound healing, reducing scar area, and improving facial scar conditions.
文摘目的:研究黄金射频微针联合注射A型肉毒毒素及外涂重组人表皮生长因子(Recombinant human epidermal growth factor,rhEGF)凝胶治疗痤疮瘢痕的效果。方法:研究者筛选出苏州和常州美莱美容医院2021年7月-2022年7月接诊的面部痤疮愈后形成瘢痕的患者共63例,并采用随机数字表法划分为研究组(n=31)和对照组(n=32)两小组。对照组选用黄金射频微针联合外涂rhEGF凝胶作为常规治疗,而研究组则在此基础上注射A型肉毒毒素联合治疗。治疗后,比较两组痤疮瘢痕修复的临床疗效、痤疮瘢痕评估量表权重评分、各种不良反应的发生情况。结果:治疗期间对照组不良反应发生率(18.75%)高于研究组(12.9%),但差异无统计学意义(P>0.05)。治疗后,研究组患者治疗后的总有效率为90.32%,高于对照组的65.63%(P<0.05)。研究组患者治疗后的ECCA评分值小于对照组(P<0.05)。结论:A型肉毒毒素注射结合黄金射频微针及rhEGF凝胶治疗痤疮瘢痕有较高的疗效,其能在保证安全性的基础上,改善痤疮后遗症。
文摘[目的]研究重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)在治疗外伤性鼓膜穿孔过程中的作用。[方法]对门诊就诊的外伤性鼓膜穿孔患者123例患者,随机的分别采用4种不同的治疗方法(即常规治疗方法、贴膜法、应用rhEGF喷剂法、贴膜和rhEGF喷剂联合应用法),定期观察鼓膜愈合情况、瘢痕的轻重,记录鼓膜完全愈合的时间,按鼓膜穿孔的大、中、小,分3组进行统计学处理。[结果]对小穿孔4种治疗方法差异无显著性意义(愈合时间,P>0.05),对中等穿孔,传统组、贴膜组与rhEGF组、贴膜+rhEGF组之间分别两两比较差异有显著性意义(P<0.05)。对大穿孔,传统组、贴膜组、贴膜+rhEGF组三者之间两两比较差异均有显著性意义(P<0.05)。[结论]外伤性鼓膜穿孔中的中等穿孔和大穿孔应用rhEGF或rhEGF与贴膜联合应用,可以显著地缩短愈合时间,并减轻鼓膜瘢痕,提高治愈率。
