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Metabolic dysfunction-associated fatty liver disease and low muscle strength: A comment
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作者 Masood Muhammad Karim Amna Subhan Butt 《World Journal of Gastroenterology》 SCIE CAS 2024年第17期2371-2373,共3页
The diagnosis of non-alcoholic fatty liver disease(NAFLD)and metabolic dysfunction-associated fatty liver disease only on the basis of laboratory parameter score such as Hepatic Steatosis Index which includes liver en... The diagnosis of non-alcoholic fatty liver disease(NAFLD)and metabolic dysfunction-associated fatty liver disease only on the basis of laboratory parameter score such as Hepatic Steatosis Index which includes liver enzymes,gender,basal metabolic index,and presence of diabetic mellitus is not sufficient to exclude other causes of deranged liver enzymes especially medications and autoimmune related liver diseases.As the guideline suggests ultrasound is the preferred first-line diagnostic procedure for imaging of NAFLD,as it provides additional diagnostic information and the combination of biomarkers/scores and transient elastography might confer additional diagnostic accuracy and evident from previous similar studies too. 展开更多
关键词 Non-alcoholic fatty liver disease Metabolic dysfunction associated fatty liver disease low muscle strength Hepatic Steatosis index Letter to the editor
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Review of Research on the Evaluation Index System of Low-carbon City in China 被引量:1
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作者 LIU Qinpu 《Journal of Landscape Research》 2016年第6期68-72,共5页
In order to promote the research on the evaluation index system of low-carbon city,this paper reviewed and summarized the current evaluation index systems in recent years and the concepts of lowcarbon city in China.Th... In order to promote the research on the evaluation index system of low-carbon city,this paper reviewed and summarized the current evaluation index systems in recent years and the concepts of lowcarbon city in China.The current evaluation index systems of low-carbon city in China could be divided into two types:two class index system and three class index system.The establishment of different index systems basically adheres to the scientific,systematic,operable and dynamic principles,and the target value and weight of evaluation index could be confirmed in multiple ways.This paper analyzed the existing problems in the evaluation index systems of low-carbon city,and came up with some suggestions and advice to promote relevant research. 展开更多
关键词 low-carbon city Evaluation index system CLASSIFICATION Comprehensive review
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Adsorption and Diffusion of Hydrogen Atom on Low-index Ag Planes
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作者 ZHANG Jing DIAO Zhao-Yu WANG Ze-Xin 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2005年第10期1217-1223,共7页
The 5-parameter Morse potential (5-MP) of the interaction between H atom and Ag surfaces has been constructed. The adsorption and diffusion of H on Ag low-index surfaces are investigated with 5-MP in detail. The cha... The 5-parameter Morse potential (5-MP) of the interaction between H atom and Ag surfaces has been constructed. The adsorption and diffusion of H on Ag low-index surfaces are investigated with 5-MP in detail. The characteristics of critical points are obtained. The theoretical results show that H atom can only adsorb at the three-fold site on Ag(111 ); the quasi-3-fold site and long-bridge site are stable adsorption sites on Ag(110) surface for the H atom, and at low coverage hydrogen predominantly occupies the quasi-3-fold site. This work predicts that the four-fold hollow site is the most stable adsorption state for H atom on Ag(100). The results of this work are approved by the experimental and theoretical results. 展开更多
关键词 H-Ag system 5-MP ADSORPTION vibration low-index surfaces
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血清IL-18、IL-22、CD4^(+)CD25^(+)CD127^(low)Treg水平联合检测对狼疮性肾炎患者活动期的诊断效能
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作者 李婕 《中国民康医学》 2024年第23期103-106,共4页
目的:分析血清白细胞介素-18(IL-18)、白细胞介素-22(IL-22)、CD4^(+)CD25^(+)CD127^(low)调节性T细胞(Treg)水平联合检测对狼疮性肾炎患者活动期的诊断效能。方法:回顾性分析2022年1月至2023年9月该院收治的64例狼疮性肾炎患者的临床资... 目的:分析血清白细胞介素-18(IL-18)、白细胞介素-22(IL-22)、CD4^(+)CD25^(+)CD127^(low)调节性T细胞(Treg)水平联合检测对狼疮性肾炎患者活动期的诊断效能。方法:回顾性分析2022年1月至2023年9月该院收治的64例狼疮性肾炎患者的临床资料,设为研究组,并根据病情严重程度将其分为活动期、稳定期,另选取同期64名健康体检者设为对照组。比较两组及不同病情严重程度患者血清IL-18、IL-22、CD4^(+)CD25^(+)CD127^(low)Treg水平,并分析各指标水平与狼疮性肾炎患者病情严重程度的相关性,采用受试者工作特征(ROC)曲线分析各指标水平单项及联合检测对狼疮性肾炎患者活动期的诊断效能。结果:研究组血清IL-18、IL-22水平均高于对照组,CD4^(+)CD25^(+)CD127^(low)Treg水平低于对照组,差异有统计学意义(P<0.05);活动期患者血清IL-18、IL-22水平均高于稳定期患者,CD4^(+)CD25^(+)CD127^(low)Treg水平低于稳定期患者,差异有统计学意义(P<0.05);Pearson相关性分析结果显示,血清IL-18、IL-22水平与狼疮性肾炎患者病情严重程度均呈正相关(r>0,P<0.05),CD4^(+)CD25^(+)CD127^(low)Treg水平与狼疮性肾炎患者病情严重程度呈负相关(r<0,P<0.05);ROC曲线分析结果显示,血清IL-18、IL-22、CD4^(+)CD25^(+)CD127^(low)Treg水平单项及联合检测诊断狼疮性肾炎患者活动期的曲线下面积分别为0.828、0.817、0.788、0.921,联合检测诊断效能高于三者单项检测。结论:IL-18、IL-22、CD4^(+)CD25^(+)CD127^(low)Treg水平联合检测诊断狼疮性肾炎患者活动期的效能高于三者单项检测。 展开更多
关键词 白细胞介素-18 白细胞介素-22 CD4^(+)CD25^(+)CD127^(low)调节性T细胞 狼疮性肾炎 活动期
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Demonstration on the indexes design of gravity satellite orbit parameters in the low-low satellite-to-satellite tracking mode 被引量:1
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作者 Liu Xiaogang Li Yingchun +1 位作者 Xiao Yun Pang Zhenxing 《Geodesy and Geodynamics》 2013年第1期29-34,共6页
Combining with the exigent demand of the development of satellite gravimetry system in China, aiming at the determination of technical indexes of gravity satellite orbit parameters, on the basis of the numerical exper... Combining with the exigent demand of the development of satellite gravimetry system in China, aiming at the determination of technical indexes of gravity satellite orbit parameters, on the basis of the numerical experiments and results analysis, the design indexes of gravity satellite orbit height, inter-satellite range and the orbit inclination are analyzed and calculated, and the issues towards twin gravity satellites such as coherence requirement of the orbit semi-major axes, control requirement of the pitch angle and time interval requirement to keep twin satellites formation in mobility are diseussed. Results show that the satellite orbit height is 400 km to 500 km, the inter-satellite range is about 220 km, the satellite orbit inclination is between polar orbit and sun-synchronous orbit, the semi-major axes difference of twin satellites orbit is within ± 70. 146 m, the pitch angle of twin satellites is about 0.9 degree, and the time interval to keep twin satellites formation in mobility is 7 days to 15 days. 展开更多
关键词 low-low satellite-to-satellite tracking (SST-11) satellite orbit height inter-satellite range orbitinclination index design
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Low-Loss, Broadband and Tunable Negative Refractive Index Metamaterial
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作者 Y.J. Huang G.J. Wen +1 位作者 T.Q. Li K. Xie 《Journal of Electromagnetic Analysis and Applications》 2010年第2期104-110,共7页
This paper report on a low-loss, broadband, and tunable negative refractive index metamaterial (NRIM) consisting of yttrium iron garnet (YIG) slabs and printed circuit boards (PCBs). The YIG slabs under an applied mag... This paper report on a low-loss, broadband, and tunable negative refractive index metamaterial (NRIM) consisting of yttrium iron garnet (YIG) slabs and printed circuit boards (PCBs). The YIG slabs under an applied magnetic field provide a negative permeability and the PCBs provide a negative permittivity. The substrates of the PCBs decuple the interactions between the YIG slabs and wire array deposed on such substrates. The effective electromagnetic parameters of the NRIM and the conditions of exhibiting the negative refractive index character are analyzed theoretically. Then the negative transmission and negative refraction characters are investigated numerically and experimentally. The results indicate that the NRIM exhibits negative pass band within the X-band with a bandwidth of about 1 GHz and a peak transmission power of about - 2.5 dB. While changing the applied magnetic field from 2300 Oe to 2700 Oe, the measured pass band of NRIM shift from 8.42 GHz to 9.50 GHz with a 2.7 MHz/Oe step. The results open a sample way to fabricate the NRIM, further, the metamaterial cloak and absorber. 展开更多
关键词 METAMATERIAL NEGATIVE Refractive index TUNABILITY low-LOSS BROADBAND
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Pain Efficacy of a Home-Based Low-Intensity Continuous Ultrasound Stimulator for Knee Arthritis: A Single-Arm, Open-Label, Prospective Clinical Trial
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作者 Md. Habibur Rahman Yeon-Gyu Jang +8 位作者 Johny Bajgai Subham Sharma Kchorng Vira Abdul-Nasir Sofian Seong Hoon Goh Yundeok Kim Cheol-Su Kim Doo-Sup Kim Kyu-Jae Lee 《Health》 2024年第7期703-716,共14页
1) Background: Osteoarthritis (OA) is defined as a degenerative joint disease that mainly affects the bone. This study aims to evaluate the effect of low-intensity continuous ultrasound (LICUS) treatment on the knee o... 1) Background: Osteoarthritis (OA) is defined as a degenerative joint disease that mainly affects the bone. This study aims to evaluate the effect of low-intensity continuous ultrasound (LICUS) treatment on the knee of osteoarthritis patients through home-based intervention using the LICUS medical device. 2) Methods: The clinical trials were designed in a single-arm, open-label, and intervention study. Thirty-five participants, including those who dropped out (12%), were screened and enrolled. The patients received LICUS (1.1 MHz, 1.5 W/cm2, collimated beams) on the knee by the instructions of the investigator at home (5 min/session, 3 times/day, for four-weeks). Outcome measures were assessed using the Visual Analog Scale (VAS) as a primary endpoint and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) as a secondary endpoint to evaluate pain relief and functional recovery of the knee between pre-treatment (baseline) and post-treatment (four-weeks). 3) Results: Knee pain scores measured using the VAS and WOMAC indices were significantly reduced after a four-week treatment with LICUS compared to baseline. Knee stiffness and functional capacity were significantly reduced after the LICUS application. In addition, there were no reports of adverse effects during the study period. 4) Conclusion: Long-term and home-based application of LICUS can be recommended as an alternative option for the treatment of OA patients, as evidenced by the effect of pain relief and knee function recovery. 展开更多
关键词 Knee Osteoarthritis low-Intensity Continuous Ultrasound Stimulator Clinical Trial Visual Analogue Scale Western Ontario and McMaster Universities Osteoarthritis index
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Study on Multi-effect Time Parameters of Ergonomic Validity Index in Low-carbon Residential Kitchen
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作者 Jun Ji Hong Jin 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第5期52-58,共7页
In the study on functional low-carbon ergonomic validity in buildings,ergonomic validity is different from resource validity which is easy for quantitative analysis. To eliminate the complexity and uncertainty impacts... In the study on functional low-carbon ergonomic validity in buildings,ergonomic validity is different from resource validity which is easy for quantitative analysis. To eliminate the complexity and uncertainty impacts of human factors on quantitative study,it proposes a method of building a parameter of ergonomic validity—multi-effect time by using cardiotachometer to record heart rate change,being used to evaluate the functional low-carbon ergonomic validity targeting at the ontological characteristics of kitchen. This method is used to determine the physical consumption intensity( multi-effect) through heart rate incremental relation based on the principles of physiology and ergonomics,and to confirm the ergonomic validity of environmental factors by the time to complete standard work as well as multi-effect quantitative analysis. The test results show that,under the kitchen operating conditions,the multi-effect( ME) can properly reflect the real-time status of the operator and is easily operated; the parameters obtained are not significantly related to the physiological status of the operator,and multi-effect time( MT) is sensitive to the physical consumption brought about to the operator due to kitchen environmental factors; thus,it can be taken as an objective index,which is simple and easy to operate in residential kitchen functional low-carbon evaluation. 展开更多
关键词 functional low-carbon human factors ergonomic validity index basal heart rate increment ratio multi-effect time of kitchen work
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Discussion and Application of Planning EIA Index System in the Industrial Development Region Based on Low Carbon Economy 被引量:1
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作者 熊鸿斌 王萍 +1 位作者 金潇 项芳 《Meteorological and Environmental Research》 CAS 2010年第12期86-90,共5页
Aimed at the low carbon economy and the characteristics of planning EIA in the industrial development region,the planning EIA index system which merged with the low carbon economy concept in the industrial development... Aimed at the low carbon economy and the characteristics of planning EIA in the industrial development region,the planning EIA index system which merged with the low carbon economy concept in the industrial development region was established.The construction planning of national eco-industrial park in Hefei High-tech Industrial Development Zone was comprehensively evaluated by using the gray correlation method.The evaluation results of considering and without considering the low carbon index were contrasted and were verified by using the improved entropy method.The results showed that the regional sustainable development index constantly rose,and the planning was reasonable.Moreover,the suggestions were also put forward to tackle the problems. 展开更多
关键词 Planning EIA in the industrial development region low carbon economy Evaluation index system China
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CD34+/CD38low/-/CD123+细胞表达水平对急性髓系白血病预后的意义 被引量:5
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作者 向立丽 邱国强 +3 位作者 谢晓宝 岑建农 胡绍燕 顾伟英 《中国实验血液学杂志》 CAS CSCD 北大核心 2017年第1期61-65,共5页
目的:探讨急性髓系白血病(AML)患者CD34^+/CD38^(low/-)/CD123^+细胞表达水平,并分析CD34^+/CD38^(low/-)/CD123^+表达水平与预后的相关性。方法:采用流式细胞术检测148例初诊AML患者骨髓单个核细胞中CD34^+/CD38^(low/-)/CD123^+表达水... 目的:探讨急性髓系白血病(AML)患者CD34^+/CD38^(low/-)/CD123^+细胞表达水平,并分析CD34^+/CD38^(low/-)/CD123^+表达水平与预后的相关性。方法:采用流式细胞术检测148例初诊AML患者骨髓单个核细胞中CD34^+/CD38^(low/-)/CD123^+表达水平,分析CD34^+/CD38^(low/-)/CD123^+细胞表达水平与完全缓解率、无病生存率和总体生存率相关性。结果:初诊AML患者骨髓细胞中CD34^+/CD38^(low/-)/CD123^+阳性细胞中位表达率为2.8%(0.01%-67%)。AML患者CD34^+/CD38^(low/-)/CD123^+高表达与NPM1野生型呈正相关(x^2=5.194,P=0.023),而与FLT3-ITD突变阳性无相关性(x^2=0.418,P>0.05)。多变量回归分析显示,CD34^+/CD38^(low/-)/CD123^+高表达与较低的完全缓解率(P=0.035)、较差的无病生存(P<0.001)、较短的总生存(P<0.001)相关。结论:CD34^+/CD38^(low/-)/CD123^+表达率与AML患者治疗的反应及生存密切相关,在临床中可做为快速识别患者治疗失败的风险预后指标。 展开更多
关键词 急性髓系白血病 CD34+/CD38low/-/CD123+ 预后
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CD4^+CD25^+CD127^(low/-)调节性T细胞在晚期胃癌中的表达及意义 被引量:13
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作者 钟锐 冯龙 +3 位作者 兰琼玉 易烽明 蒋为民 吴建兵 《重庆医学》 CAS 北大核心 2016年第15期2065-2069,共5页
目的研究CD4^+CD25^+CD127low/-调节性T细胞(Treg)在晚期胃癌患者外周血中的表达水平及其对化疗疗效判断的意义。方法采用流式细胞术(FCM)检测30例晚期胃癌患者和10例健康体检者外周血中CD4^+CD25^+CD127low/-Treg的表达水平。结果晚期... 目的研究CD4^+CD25^+CD127low/-调节性T细胞(Treg)在晚期胃癌患者外周血中的表达水平及其对化疗疗效判断的意义。方法采用流式细胞术(FCM)检测30例晚期胃癌患者和10例健康体检者外周血中CD4^+CD25^+CD127low/-Treg的表达水平。结果晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg占CD4^+T淋巴细胞的百分比为(10.41±2.85)%,明显高于健康体检者(5.05±1.32)%(t=8.04,P<0.01),也明显高于化疗2周期后(7.07±1.45)%,(t=5.72,P<0.01)和化疗4周期后(7.30±1.65)%(t=5.17,P<0.01)。晚期胃癌患者化疗2周期后外周血中CD4^+CD25^+CD127low/-Treg占CD4^+T淋巴细胞的百分比为(7.07±1.45)%,与化疗4周期后(7.30±1.65)%比较,差异无统计学意义(t=0.57,P>0.05)。晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg表达水平与患者性别、年龄、胃癌相关肿瘤抗原及肿瘤部位无相关性(P>0.05),低分化程度与中分化程度差异无统计学意义(P>0.05)。晚期胃癌患者化疗2周期后缓解组(完全缓解^+部分缓解)外周血CD4^+T细胞中CD4^+CD25^+CD127low/-Treg的水平为(6.25±1.08)%与化疗2周期后未缓解组(病变稳定^+疾病进展)的(7.98±1.27)%比较,差异有统计学意义(t=4.02,P<0.01)。结论晚期胃癌患者外周血中CD4^+CD25^+CD127low/-Treg的表达水平与肿瘤缓解情况有关。 展开更多
关键词 胃肿瘤 T淋巴细胞 流式细胞术 化疗 晚期胃癌 CD4+CD25+CD127low/-调节性T细胞
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E-cadherin、N-cadherin与CD44^+/CD24^(-/low)表型在乳腺癌中表达的相关性及其意义 被引量:16
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作者 包俊杰 吴诚义 《中国癌症杂志》 CAS CSCD 北大核心 2010年第8期596-601,共6页
背景与目的:上皮细胞-间质转化(epithelial-mesenchymal transition,EMT)可以诱导上皮细胞来源的肿瘤表达CD44+/CD24-/low表型细胞,上皮性钙黏蛋白(E-cadherin)和神经性钙黏蛋白(N-cadherin)是分别表达于上皮细胞和间质细胞的两个重要... 背景与目的:上皮细胞-间质转化(epithelial-mesenchymal transition,EMT)可以诱导上皮细胞来源的肿瘤表达CD44+/CD24-/low表型细胞,上皮性钙黏蛋白(E-cadherin)和神经性钙黏蛋白(N-cadherin)是分别表达于上皮细胞和间质细胞的两个重要钙黏蛋白,癌细胞从E-cadherin表达向N-cadherin表达转移是EMT的一个重要机制,此钙黏蛋白转移增加了癌细胞的侵袭转移性。本研究旨在探讨CD44+/CD24-/low表型、E-cadherin及N-cadherin在乳腺癌中的表达及其意义。方法:采用免疫组织化学检测15例正常乳腺组织、20例乳腺癌癌旁组织(癌边缘2~5cm)、58例乳腺癌组织及18例腋窝转移淋巴结组织中CD44+/CD24-/low表型、E-cadherin及N-cadherin的表达,分析其与乳腺癌发生及浸润转移的关系。结果:正常乳腺组织、乳腺癌癌旁组织、乳腺癌组织和腋窝转移淋巴结组织中CD44+/CD24-/low表型表达率分别为6.7%、10.0%、41.4%和44.4%,E-cadherin阳性表达率分别为100%、90.0%、51.7%和33.3%,N-cadherin阳性表达率分别为0、15.0%、44.8%和55.6%,3种蛋白在正常乳腺组织、乳腺癌癌旁组织、乳腺癌组织和腋窝转移淋巴结组织中的表达差异均有统计学意义(P<0.05)。CD44+/CD24-/low表型表达与E-cadherin及N-cadherin表达有显著差异,且与E-cadherin表达关联程度较强,与N-cadherin表达关联程度相对较弱。E-cadherin表达与N-cadherin表达有显著差异,且具有高度相关性(P<0.05)。结论:CD44+/CD24-/low表型表达增加、E-cadherin蛋白表达降低及N-cadherin蛋白反常表达与乳腺癌的发生及浸润转移有密切的关系;在乳腺癌发展过程中,E-cadherin向N-cadherin转化及CD44+/CD24-/low表型表达增加可能发挥重要作用。 展开更多
关键词 乳腺癌 CD44+/CD24-/low表型 E-CADHERIN蛋白 N-cadherin蛋白 双重免疫组织 化学染色
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B淋巴细胞白血病骨髓B细胞CD34^+CD38^+和CD34^+CD38^(low/-)细胞亚群的临床意义 被引量:3
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作者 郝乐 刘艳荣 +5 位作者 王亚哲 常艳 秦亚溱 李金兰 李玲娣 黄晓军 《中国实验血液学杂志》 CAS CSCD 北大核心 2012年第4期801-805,共5页
本研究比较发病时免疫表型为CD34+CD38+和CD34+CD38low/-两组B淋巴细胞白血病(B-ALL)的生物学特性,并探讨其临床意义。选取54例初发B-ALL并经多参数流式细胞术检测为CD34+的B-ALL患者,根据CD38表达不同将其分为CD34+CD38+组(n=35)和CD34... 本研究比较发病时免疫表型为CD34+CD38+和CD34+CD38low/-两组B淋巴细胞白血病(B-ALL)的生物学特性,并探讨其临床意义。选取54例初发B-ALL并经多参数流式细胞术检测为CD34+的B-ALL患者,根据CD38表达不同将其分为CD34+CD38+组(n=35)和CD34+CD38low/-组(n=19)。利用实时定量PCR的方法分别进行BCR-ABL,TEL-AML1和WT1基因的检测。随访标本利用7色流式细胞术检测微量残留病(MRD),平均随访时间12个月(1-28个月),平均随访间隔2个月(1-5个月)。结果表明,两组患者发病时WBC,血小板和血红蛋白水平及BCR-ABL,TEL-AML1和WT1基因阳性表达的比例均无统计学差异(P>0.05)。在诱导缓解后,CD34+CD38+组微量残留病阳性(MRD+)为28.57%(10/35),CD38low/-组MRD+为68.42%(13/19),CD34+CD38low/-组MRD+的检出率明显高于CD34+CD38+组(P<0.01)。在复发率上,CD34+CD38+组有2例,分别在第94天和第245天复发,复发率为5.71%(2/35)。CD34+CD38low/-组有7例复发,复发率为36.84%(7/19),中位复发时间263 d(46-468 d),两组间具有明显统计学差异(P<0.01)。本研究以16岁为年龄分界点,CD34+CD38+组16岁以上患者为8人(8/35),CD34+CD38low/-组16岁以上患者为10人(10/19),两组间有统计学差异(P<0.05)。结论:CD34+CD38low/-患者在成人中比例较高,且CD34+CD38low/-组在治疗后更易出现MRD+和复发。 展开更多
关键词 CD34+CD38+ CD34+CD38low/- B淋巴细胞白血病 微小残留病
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CD4^+ CD25^(high) CD127^(low/-)调节性T细胞在多发性骨髓瘤患者外周血的表达 被引量:3
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作者 高鹏 王雪野 +6 位作者 肖中平 付堃 孔令环 王雪梅 李凌君 封韬 韩梅 《中国老年学杂志》 CAS CSCD 北大核心 2014年第20期5673-5675,共3页
目的探讨多发性骨髓瘤(MM)患者外周血CD4+CD25highCD127low/-调节性T(Tregs)细胞表达水平及临床意义。方法采用流式细胞术(FCM)检测30例MM患者和20例健康对照者外周静脉血CD4+CD25highCD127low/-Tregs细胞水平。结果 1 MM组CD4+CD25high... 目的探讨多发性骨髓瘤(MM)患者外周血CD4+CD25highCD127low/-调节性T(Tregs)细胞表达水平及临床意义。方法采用流式细胞术(FCM)检测30例MM患者和20例健康对照者外周静脉血CD4+CD25highCD127low/-Tregs细胞水平。结果 1 MM组CD4+CD25high CD127low/-Tregs细胞表达明显高于正常对照组(P<0.05)。2MM组不同国际分期系统(ISS)分期CD4+CD25highCD127low/-Treg细胞水平均高于正常对照组,随着疾病进展,Ⅲ期明显高于Ⅱ期(P<0.05)。3MM组化疗后外周血CD4+CD25highCD127low/-Tregs细胞水平较化疗前显著下降(P<0.05)。结论 MM患者外周血CD4+CD25highCD127low/-Treg细胞比例明显升高,可能是MM免疫逃逸的一个重要机制;进展期Tregs的变化为MM患者及时进行临床干预提供实验依据。 展开更多
关键词 多发性骨髓瘤 CD4+CD25highCD127low/-调节性T细胞 流式细胞术
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慢性乙型肝炎肝纤维化患者外周血CD4^+CD25^+CD127^(low/-) T细胞与人肝星状细胞共培养的实验研究 被引量:4
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作者 金晓芝 陈永平 +4 位作者 程瑗 潘黎静 潘陈为 戴志娟 叶超 《医学研究杂志》 2011年第8期70-73,共4页
目的探讨CD4+CD25+CD127low/-T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例;采用磁激活细胞分选法(MACS)提取13例患者和6例健康对照者外周血CD4+CD25... 目的探讨CD4+CD25+CD127low/-T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例;采用磁激活细胞分选法(MACS)提取13例患者和6例健康对照者外周血CD4+CD25+CD127low/-T细胞;分别将CD4+CD25+CD127low/-T细胞以不同比例与人肝星状细胞株(hepaticstellate cell,HSC)共培养5天,检测各组HSC的增殖及TGF-β1的分泌。结果 CD4+CD25+CD127low/-T细胞与HSC之比,当健康对照组和S1~S2期组为1.5∶1,S3~S4期组为2∶1时,HSC的增殖速度和TGF-β1分泌量均在同组不同比例中达最高值。各组同比例之间,当比例为1.5∶1时,健康对照组HSC的增殖速度显著高于S3~S4期组(P<0.05);在比例为1∶1~2∶1范围内,S1~S2期组和S3~S4期组HSC的TGF-β1分泌量远高于同比例健康对照组(P<0.05)。结论 CD4+CD25+CD127low/-T细胞可能通过自身细胞数量和功能的双重调节,影响肝星状细胞的增殖和分泌,参与了慢乙肝肝纤维化的进展。 展开更多
关键词 慢性乙型病毒性肝炎 肝纤维化 CD4+CD25+CD127low/- Treg MACS
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HIV感染者外周血CD4^+ CD25^+ Foxp3^+ /CD127^(low/-)调节性T细胞表达水平对疾病进展的影响 被引量:6
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作者 陈远林 陈新瑞 +2 位作者 秦立新 杨一芬 唐亚梅 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第11期1188-1191,1193,共5页
目的:探讨HIV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)、CD4+CD25+CD127low/-Treg的水平及其与其他免疫指标的关系。方法:采集68例未经抗HIV治疗的HIV/AIDS患者(长期不进展组即LTNP组29例、典型进展的HIV感染组27例、AIDS组12例... 目的:探讨HIV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)、CD4+CD25+CD127low/-Treg的水平及其与其他免疫指标的关系。方法:采集68例未经抗HIV治疗的HIV/AIDS患者(长期不进展组即LTNP组29例、典型进展的HIV感染组27例、AIDS组12例)及20例健康成人的外周抗凝全血,经免疫荧光染色,应用流式细胞仪分析CD4+T细胞、CD8+T细胞、NK细胞及CD4+CD25+Foxp3+/CD127low/-Treg的含量,并进行统计学分析。结果:除CD8+T细胞外,HIV/AIDS患者外周血中CD4+T细胞、NK细胞、CD4+/CD8+结果均明显低于健康对照组(P<0.05);随着疾病的进展,LTNP组、HIV组、AIDS组CD4+T细胞百分比、绝对值计数,CD8+T细胞绝对计数,NK细胞绝对计数,CD4+/CD8+比值逐渐下降,而CD8+T细胞百分比逐渐上升。对CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg百分含量、绝对计数进行多重比较发现,各组间CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg所占CD4+T细胞百分含量的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg细胞百分含量逐渐上升,LTNP组与健康对照组之间、HIV组和AIDS组之间CD4+CD25+Foxp3+/CD127low/-Treg绝对计数差异无统计学意义(P>0.05),其余各组间的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg绝对计数逐渐下降。结论:CD4+CD25+Foxp3+/CD127low/-Treg在HIV持续感染的免疫发病机制中有一定作用。 展开更多
关键词 HIV CD4+CD25+Foxp3+调节性T细胞 CD4+CD25+CD127low/-调节性T细胞 长期不进展者
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三阴乳腺癌CD44^+/CD24^(-/LOW)细胞与CXCR4表达的临床意义 被引量:1
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作者 何惠华 杨继洲 +1 位作者 李蓓 敖启林 《中国组织化学与细胞化学杂志》 CAS CSCD 2014年第2期190-194,共5页
目的探讨CD44+/CD24-/low表型在三阴乳腺癌中的临床病理意义以及与CXCR4表达的相关性。方法根据ER、PR、Her-2表达情况将118例乳腺癌分为三阴及非三阴组,应用免疫组织化学检测其CD44、CD24及CXCR4表达情况,分析CD44+/CD24-/low表型及CX... 目的探讨CD44+/CD24-/low表型在三阴乳腺癌中的临床病理意义以及与CXCR4表达的相关性。方法根据ER、PR、Her-2表达情况将118例乳腺癌分为三阴及非三阴组,应用免疫组织化学检测其CD44、CD24及CXCR4表达情况,分析CD44+/CD24-/low表型及CXCR4在三阴乳腺癌中表达情况及其与临床病理的关系。结果 34例三阴乳腺癌肿瘤细胞CD44+/CD24-/low表型阳性率为55.9%,CD44+/CD24-/low阳性与一般临床特征不相关,但与远处转移或复发密切相关。CXCR4的表达与CD44+/CD24-/low阳性细胞成正相关。结论三阴乳腺癌的复发和远处转移可能与CD44+/CD24-/low较高的表达率有关,并且可能是由CXCR4介导的。 展开更多
关键词 三阴乳腺癌 CD44+/CD24-/low表型细胞 CXCR4蛋白 随访
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类风湿关节炎患者CD4^+CD25^+CD127^(LOW/-)调节性T细胞检测及意义 被引量:4
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作者 张智贤 曾桦 《检验医学与临床》 CAS 2013年第22期2984-2985,2987,共3页
目的研究CD4+CD25+CD127 LOW/-调节性T细胞(Tregs)在类风湿关节炎患者(RA)外周血中的比例改变,并探讨其在疾病进程中的意义。方法选取活动期及稳定期RA患者,采用流式细胞术,检测外周血中CD4+CD25+CD127 LOW/-Treg细胞占CD4+细胞的比例,... 目的研究CD4+CD25+CD127 LOW/-调节性T细胞(Tregs)在类风湿关节炎患者(RA)外周血中的比例改变,并探讨其在疾病进程中的意义。方法选取活动期及稳定期RA患者,采用流式细胞术,检测外周血中CD4+CD25+CD127 LOW/-Treg细胞占CD4+细胞的比例,并与健康者进行比较。结果 RA患者CD4+CD25+细胞所占比例与健康对照组差异无统计学意义;而活动期患者外周血CD4+CD25+CD127 LOW/-Treg细胞明显低于稳定期组和健康对照组(P<0.05)。结论类风湿性关节炎活动期时CD4+CD25+CD127 LOW/-Treg细胞明显减少,这群调节性T细胞可能参与了类风湿性关节炎的疾病进程。 展开更多
关键词 I类风湿性关节炎 流式细胞术 CD4+CD25+CD127low -调节性T细胞
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CD44^+/CD24^(-/low)在乳腺癌中的表达及其与含蒽环类药物化疗方案敏感度的关系 被引量:1
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作者 周鑫 吴诚义 《肿瘤防治研究》 CAS CSCD 北大核心 2012年第8期966-969,共4页
目的研究乳腺癌中CD44+/CD24-/low的表达及其与含蒽环类药物化疗方案敏感度的关系。方法 91例乳腺癌接受含蒽环类药物的术前新辅助化疗,2~4个疗程后进行效果评价;采用双染免疫组织化学方法检测化疗前后CD44+/CD24-/low的表达,t检验分... 目的研究乳腺癌中CD44+/CD24-/low的表达及其与含蒽环类药物化疗方案敏感度的关系。方法 91例乳腺癌接受含蒽环类药物的术前新辅助化疗,2~4个疗程后进行效果评价;采用双染免疫组织化学方法检测化疗前后CD44+/CD24-/low的表达,t检验分析化疗前后CD44+/CD24-/low细胞比例的变化,χ2检验分析乳腺癌CD44+/CD24-/low表型与乳腺癌临床病理参数及化疗疗效的关系。结果乳腺癌中CD44+/CD24-/low阳性表达率为39.6%(36/91),在接受含蒽环类药物的新辅助化疗后乳腺癌中CD44+/CD24-/low细胞比例较化疗前明显增加(P=0.028)。CD44+/CD24-/low阳性组ER阳性率明显低于CD44+/CD24-/low阴性组(25.0%vs.47.3%,P=0.033)。三阴性乳腺癌中CD44+/CD24-/low阳性率明显高于非三阴性乳腺癌(61.9%vs.32.9%,P=0.017)。CD44+/CD24-/low表型与年龄、肿瘤大小、临床分期、病理类型、组织学分级等乳腺癌临床病理参数无明显关系(P>0.05)。CD44+/CD24-/low阳性组的总有效率高于CD44+/CD24-/low阴性组,但两组之间差异无统计学意义(75%vs.69.1%,P=0.542);CD44+/CD24-/low阳性组的病理完全缓解率明显高于CD44+/CD24-/low阴性组(38.9%vs.18.2%,P=0.028)。结论 CD44+/CD24-/low细胞仅存在于部分乳腺癌,CD44+/CD24-/low表型与ER(﹣)、三阴性乳腺癌相关,CD44+/CD24-/low表型与乳腺癌对含蒽环类药物化疗方案的敏感度相关,CD44+/CD24-/low表型可能成为乳腺癌临床化疗疗效的预测指标之一。 展开更多
关键词 乳腺癌 CD44+/CD24-/low表型 三阴性 化疗敏感度
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肾移植患者外周血中的CD4~+ CD25~+ CD127^(low/-)调节性T细胞 被引量:1
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作者 陈远林 曾钰钦 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2011年第44期8209-8213,共5页
背景:越来越多的实验在分析免疫耐受标志,以期能够更好地辅助患者进行移植后免疫抑制治疗。目的:分析肾移植后患者外周血中CD4+CD25+CD127low/-调节性T细胞在肾移植免疫耐受中的作用。方法:采集62例肾移植后患者(急性排斥反应组22例,移... 背景:越来越多的实验在分析免疫耐受标志,以期能够更好地辅助患者进行移植后免疫抑制治疗。目的:分析肾移植后患者外周血中CD4+CD25+CD127low/-调节性T细胞在肾移植免疫耐受中的作用。方法:采集62例肾移植后患者(急性排斥反应组22例,移植稳定组40例)及20例健康对照者的外周抗凝血,经免疫染色,应用流式细胞仪分析CD4+CD25+CD127low/-调节性T细胞所占CD4+T细胞百分含量,同时采用ELISA方法检测患者血清中白细胞介素2和白细胞介素10的质量浓度。结果与结论:移植稳定组中CD4+CD25+CD127low/-调节性T细胞所占CD4+T细胞百分含量显著高于健康对照组和急性反应排斥组(P<0.01);CD4+CD25+CD127low/-调节性T细胞百分含量与白细胞介素2呈显著负相关(P<0.05),与白细胞介素10呈显著正相关(P<0.01)。提示CD4+CD25+CD127low/-调节性T细胞在肾移植后免疫耐受的机制中发挥了一定作用。 展开更多
关键词 肾移植 CD4+CD25+CD127low/-调节性T细胞 白细胞介素2 白细胞介素10 免疫耐受
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