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西方蜜蜂Lozenge基因的克隆鉴定及时空表达分析
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作者 黄蕾 朱雅楠 +5 位作者 梁立强 李秋方 李正汉卿 林焱 聂红毅 苏松坤 《环境昆虫学报》 CSCD 北大核心 2023年第1期133-141,共9页
Lozenge蛋白(Lz蛋白)是昆虫的重要转录因子,在昆虫胚胎发育过程中发挥重要作用。为研究Lozenge在西方蜜蜂Apis mellifera中的作用,本研究克隆了Lozenge基因,并对其进行生物信息学分析,同时基于荧光定量PCR技术检测该基因在西方蜜蜂不同... Lozenge蛋白(Lz蛋白)是昆虫的重要转录因子,在昆虫胚胎发育过程中发挥重要作用。为研究Lozenge在西方蜜蜂Apis mellifera中的作用,本研究克隆了Lozenge基因,并对其进行生物信息学分析,同时基于荧光定量PCR技术检测该基因在西方蜜蜂不同发育时期(卵期、幼虫期、蛹期和成年蜂)和10日龄哺育蜂各组织的表达谱。生物信息学分析结果显示,Lozenge基因的开放阅读框(ORF)为1 554 bp,共编码517个氨基酸,预测分子量为54.63918 kDa,等电点为6.08;结构域预测分析发现Lozenge蛋白含有一个Runt结构域,多物种蛋白序列对比发现该蛋白同源性高。时期表达谱表明,该基因在第1日卵和第2日卵的表达量远高于其他时期,在卵期表达量随时间依次递减,幼虫期表达量极低,蛹期表达量呈先增后减的趋势,而成年蜂中均有表达;组织表达谱显示,该基因在哺育蜂头部、上颚腺中的表达量较高,而在腹部的表达量低。这些结果表明,Lozenge基因可能在西方蜜蜂胚胎期细胞发育过程、哺育蜂蜂王浆合成和分泌过程中发挥重要作用,这些结果为该基因功能的深入研究提供了重要的理论参考。 展开更多
关键词 lozenge RUNX家族 西方蜜蜂 表达分析 基因克隆
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Optimization of the Formula of Maca Lozenges by Orthogonal Test
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作者 徐天才 杨少华 +2 位作者 赵菊 谭敬菊 薛润光 《Agricultural Science & Technology》 CAS 2014年第6期994-998,1019,共6页
The orthogonal experimental design was used to optimize the formula of maca lozenge and explore the preparation techniques and product property of maca lozenges. With the sensory evaluation of maca lozenge as the indi... The orthogonal experimental design was used to optimize the formula of maca lozenge and explore the preparation techniques and product property of maca lozenges. With the sensory evaluation of maca lozenge as the indicator, L16 (44) orthogonal design graph was used to select the optimal techniques, and to investigate the stability, heavy metals and microorganisms contents in the tablets. The optimal formula was 80% of maca power, 12% of Rhodiola rosea powder, and 6% of Angelica sinensis powder, where the comprehensive sensory index reached 246. Moreover, under the optimal conditions, various kinds of heavy metals contents were in consistent with the GB164740 standards, and there was no significant change in color, size, friability, and disintegration time, as well as microorganism content during the 12-month preservation. With simple, easy and reliable technique and quality control method, the process study had obtained the national patent process (patent number: ZL200810233796.X), and the contents of heavy metals and micr6organisms laid foundation for the quality standard. 展开更多
关键词 Maca lozenge Rhodiola rosea Orthogonal design Sensory evaluation
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Throat Lozenges and Spray Containing Chlorhexidine and Lidocaine Fixed Combination Show Virucidal Activity against Respiratory Syncytial Virus and SARS-CoV-2
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作者 Annalisa Cantatore Delyth Whiteford +1 位作者 Adam Pitz Andrew Middleton 《Open Journal of Respiratory Diseases》 2024年第4期100-110,共11页
The limitations of existing treatments for both Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome-related Coronavirus 2 (SARS-CoV-2) lie in their inability to provide universally accessible, easy... The limitations of existing treatments for both Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome-related Coronavirus 2 (SARS-CoV-2) lie in their inability to provide universally accessible, easy-to-use, and effective solutions. A commercially available fixed combination of chlorhexidine and lidocaine in both, lozenge and spray form, were assessed for their antiviral efficacy against RSV and SARS-CoV-2 in a suspension test, the viral titres were measured by standard TCID50. Both formulations were able to reduce the RSV titre to undetectable levels (99.9% virus inactivation, 3 log10 reduction) in less than 1 minute. The lozenge formulation inactivated the viral activity of SARS-CoV-2 in 5 minutes (99% virus inactivation, 2 log10 reduction), while the spray formulation led to a reduction of SARS-CoV-2 titre to undetectable levels in less than 1 minute (99.9%, 3 log10 reduction). In conclusion, our results show that preparations combining chlorhexidine and lidocaine significantly reduce certain respiratory viruses in vitro. In this regard, physiological effects of these preparations become more obvious potentially affecting viral transmission to other individuals and spreading to the lower respiratory tract—thereby shortening the duration and severity of symptoms. 展开更多
关键词 Throat lozenge Oromucosal Spray Respiratory Syncytial Virus SARS-CoV-2
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Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein 被引量:1
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作者 Kui Zhang Juan Tan +5 位作者 Xiangwei Hao Houyi Tang Muhammad Nadeem Abbas Jingjing Su Yongyue Su Hongjuan Cui 《Insect Science》 SCIE CAS CSCD 2022年第3期704-716,共13页
Zinc finger protein,an important transcription factor,regulates gene expression associated with various physiological and pathological processes.U-shaped,belong to the Friend of GATA(FOG)transcription factor,plays a c... Zinc finger protein,an important transcription factor,regulates gene expression associated with various physiological and pathological processes.U-shaped,belong to the Friend of GATA(FOG)transcription factor,plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co-factor.However,little is known about its functions in insects.In the present study,a U-shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity investigated.The predicted silkworm U-shaped amino acid sequence contained a classical nuclear localization signal(NLS)motif“GESSPKRRRR”at position 45CU459,and arginine residues at position 456 and 478 are the critical sites of the NLS.U-shaped mRNA was detected in all tested tissues of the B.mori;however,the highest levels were found in the hemocytes.U-shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus challenge.Furthermore,U-shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization-associated genes,including PPO1,PPO2,PPAE and BAEE.In addition,U-shaped interacts with Lozenge protein to regulate the innate immune response of the insect.Our results revealed that U-shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm. 展开更多
关键词 Bombyx mori lozenge melanization response SILKWORM U-SHAPED
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The Maximum Effective Moment Criterion (MEMC) and Its Implications in Structural Geology 被引量:15
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作者 ZHENG Yadong WANG Tao WANG Xinshe 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2006年第1期70-78,共9页
The MohroCoulomb criterion has been widely used to explain formation of fractures. However, it fails to explain large strain deformation that widely occurs in nature. There is presently a new theory, the MEMC, which i... The MohroCoulomb criterion has been widely used to explain formation of fractures. However, it fails to explain large strain deformation that widely occurs in nature. There is presently a new theory, the MEMC, which is mathematically expressed as Meff = ((σ1-σ3) L.sin 2α sin α)/2, where σ1-σ3 represents the yield strength of the related rock, L is a unit length and a is the angle between σ1 and deformation bands. This criterion demonstrates that the maximum value appears at angles of ±54.7° to σ1 and there is a slight difference in the moment in the range of 55°±10°. The range covers the whole observations available from nature and experiments. Its major implications include: (1) it can be used to determine the stress state when the related deformation features formed; (2) it provides a new approach to determine the Wk of the related ductile shear zone if only the ratio of the vorticity and strain rate remains fixed; (3) It can be used to explain (a) the obtuse angle in the contraction direction of conjugate kink-bands and extensional crenulation cleavages, (b) formation of low-angle normal faults and high-angle reverse faults, (c) lozenge ductile shear zones in basement terranes, (d) some crocodile structures in seismic profiles and (e) detachment folds in foreland basins. 展开更多
关键词 maximum effective moment criterion crenulation cleavage low-angle normal fault high-angle reverse fault crocodile structure lozenge structure detachment fold
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