This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection （MAS）. An F2 population developed from the cross between the resistant line TcLr24 an...This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection （MAS）. An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC1ss bp, P-AGC/M- CAC162 bp, and P-ACG/M-CGC239 bp, were co-segregated with Lr24. The AFLP fragment from the primer combination P- ACG/M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS13026oz developed by Gupta. 5R615, 5R616, IR13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.展开更多
Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat worldwide.Deploying resistant varieties against leaf rust is the most effective,environmentally-friendly and econ...Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat worldwide.Deploying resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the disease.In the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the greenhouse.All the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping seasons.Moreover,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the varieties.The results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 varieties.Known Lr genes were not identified in the remaining 34 varieties.Seventeen varieties were found to have slow rusting resistance.The resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.展开更多
To study actions of the genes associated with tight junction, adherent junction, focal adhesion, and gap junction during liver regeneration （LR）, these genes were obtained by collecting data from databases and thesi...To study actions of the genes associated with tight junction, adherent junction, focal adhesion, and gap junction during liver regeneration （LR）, these genes were obtained by collecting data from databases and thesis, and their expression profiles in rat regenerating liver were detected employing Rat Genome 230 2.0 array. Next the LR-associated genes were identified by comparing the difference between sham operation （SO） and partial hepatectomy （PH） groups. 79, 53, 109, 53 genes involved in the above four junctions were found to be LR-associated. The initial and total expression numbers of these genes occurring in the initial phase of LR, G0/G1, cell proliferation, cell differentiation, and structure-functional rebuilding were 124, 43, 122, 10, and 249, 145, 957, 306, respectively, illustrating that genes were initi^ly expressed mainly in the initiation stage, and functioned in different phases. Up-regulation-and down-regulation to a total of 972 and 540 times, as well as, 41 types of expression patterns showed that the physiological and biochemical activities were diverse and complicated in LR. According to the data, there was an increase in the forepart and prophase, but a decrease in late-metaphase and anaphase for gap junction assembly. Focal adhesion formation displayed an enhancement in forepart, prophase, and anaphase; and formation of tight junctions and adherent junctions last throughout the LR.展开更多
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobac...Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.展开更多
基金the National Natural Science Foundation of China (30771391)the transgenic Major Projects,China (2009ZX08002-012B)the Specialized Research Fund for the Doctoral Program of Higher Education of China (20101302120005) for funding the research project
文摘This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection （MAS）. An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC1ss bp, P-AGC/M- CAC162 bp, and P-ACG/M-CGC239 bp, were co-segregated with Lr24. The AFLP fragment from the primer combination P- ACG/M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS13026oz developed by Gupta. 5R615, 5R616, IR13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.
基金the National Key Research and Development Program of China(2017YFD0300906-07)。
文摘Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat worldwide.Deploying resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the disease.In the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the greenhouse.All the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping seasons.Moreover,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the varieties.The results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 varieties.Known Lr genes were not identified in the remaining 34 varieties.Seventeen varieties were found to have slow rusting resistance.The resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.
基金the National Basic Research 973 Pre-research Program of China (No. 2006CB708506).
文摘To study actions of the genes associated with tight junction, adherent junction, focal adhesion, and gap junction during liver regeneration （LR）, these genes were obtained by collecting data from databases and thesis, and their expression profiles in rat regenerating liver were detected employing Rat Genome 230 2.0 array. Next the LR-associated genes were identified by comparing the difference between sham operation （SO） and partial hepatectomy （PH） groups. 79, 53, 109, 53 genes involved in the above four junctions were found to be LR-associated. The initial and total expression numbers of these genes occurring in the initial phase of LR, G0/G1, cell proliferation, cell differentiation, and structure-functional rebuilding were 124, 43, 122, 10, and 249, 145, 957, 306, respectively, illustrating that genes were initi^ly expressed mainly in the initiation stage, and functioned in different phases. Up-regulation-and down-regulation to a total of 972 and 540 times, as well as, 41 types of expression patterns showed that the physiological and biochemical activities were diverse and complicated in LR. According to the data, there was an increase in the forepart and prophase, but a decrease in late-metaphase and anaphase for gap junction assembly. Focal adhesion formation displayed an enhancement in forepart, prophase, and anaphase; and formation of tight junctions and adherent junctions last throughout the LR.
基金supported by the National Natural Science Foundation of China (No. 21177009)
文摘Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.
