The present paper investigates the pupal development times ofLucilia sericata which were studied in the laboratory at six different constant temperatures （20, 22, 24, 26, 28 ℃ each ± ℃）. Lower thresholds （tL...The present paper investigates the pupal development times ofLucilia sericata which were studied in the laboratory at six different constant temperatures （20, 22, 24, 26, 28 ℃ each ± ℃）. Lower thresholds （tL） for development were estimated from the linear regression of the developmental rates on each temperature. These data have made it possible to calculate the ADD （Accumulated Degree-Days） necessary for L. sericata to complete the larval stage and to achieve adult emergence. The minimal duration of development from oviposition to adult emergence was found to be inversely related to temperature. Additionally, six landmarks in pupal development are showed and for each of the landmarks the ADD value was calculated for every rearing temperature involved. These data assist in calculating the duration of the pupal stage based on morphological characteristics and would be of great value for future forensic entomological casework.展开更多
Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling...Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,2010.The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic keys.Results:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties.In the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,respectively.Conclusions:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.展开更多
Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic injury.Mettiods:In our preliminary study we have shown that injuring the maggots with a needle soaked in ...Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic injury.Mettiods:In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands.It is assumed that lucifensin is secreted solely from this tissue into the haemoiymph(similar to other insect defensins) and not into secreted/excreted products.We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemoiymph extracted from larvae after septic injury.Results:After septic injury,production of lucifensin in the haemoiymph is increased.This led to higher antibacterial activity of such haemoiymph in comparison to non-stimulated larvae.Coclusions:These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy,lucifensin is simultaneously important as a part of the systematic immune response.展开更多
文摘The present paper investigates the pupal development times ofLucilia sericata which were studied in the laboratory at six different constant temperatures （20, 22, 24, 26, 28 ℃ each ± ℃）. Lower thresholds （tL） for development were estimated from the linear regression of the developmental rates on each temperature. These data have made it possible to calculate the ADD （Accumulated Degree-Days） necessary for L. sericata to complete the larval stage and to achieve adult emergence. The minimal duration of development from oviposition to adult emergence was found to be inversely related to temperature. Additionally, six landmarks in pupal development are showed and for each of the landmarks the ADD value was calculated for every rearing temperature involved. These data assist in calculating the duration of the pupal stage based on morphological characteristics and would be of great value for future forensic entomological casework.
文摘Objective:To carry out an experimental study with the main objective of mass rearing of sheep flies(Lucilia sericata).Methods:Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April,2010 to November,2010.The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality,they were kept under insectary condition to develop to adult stage and identified using systematic keys.Results:A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties.In the first generation,433 flies including 135(31.17%) male,and 298(68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas,whereas it was 2,20%and 1.81%,respectively in Fl and F2 generations,respectively.Conclusions:During this study,the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy.
基金Supported by the Operational Program Research and Development and co-financed by the European Fund for Regional Development(EFRD)via Grant:ITMS 26240220030-"Research and development of new biotherapeutic methods and its application in some illnesses treatment"
文摘Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic injury.Mettiods:In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands.It is assumed that lucifensin is secreted solely from this tissue into the haemoiymph(similar to other insect defensins) and not into secreted/excreted products.We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemoiymph extracted from larvae after septic injury.Results:After septic injury,production of lucifensin in the haemoiymph is increased.This led to higher antibacterial activity of such haemoiymph in comparison to non-stimulated larvae.Coclusions:These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy,lucifensin is simultaneously important as a part of the systematic immune response.
