[Objective] The research aimed to understand the genetic control of seed germination characters in dicotyledon plant using a suitable genetic model. [Method] An investigation of three seedling traits of seven parents ...[Objective] The research aimed to understand the genetic control of seed germination characters in dicotyledon plant using a suitable genetic model. [Method] An investigation of three seedling traits of seven parents and their F1s was undertaken in a diallel cross of sponge gourd （Luffa cylindrical Roem.） by germinating the seeds in two environments. A genetic model is developed to analyze the embryo effects as well as genotype × environment （GE） interaction effects of germination characters. [Result] Variations of the root length, shoot length and shoot dry weight were affected by genetic effects and environmental interaction effects. The three seedling traits were mainly controlled by genetic effect. Embryo dominance effects were the main genetic effect for root length and shoot length. Among the GE interaction effects, there only existed embryo dominance interaction effects for the three seedling traits. General narrow-sense heritabilities of shoot length and shoot dry weight were 7.0% and 27.3%, respectively. [Conclusion] The present modified genetic model could be used for investigating the genetic control of seed germination characters and seedling traits in dicotyledon plant.展开更多
选择合适的内参基因是提高实时荧光定量PCR分析(q RT-PCR)准确性的重要条件。18S r RNA基因表达范围广、表达量恒定,常作为内参基因应用于实时荧光定量PCR中。为了获得丝瓜18S r RNA基因,并设计合适的荧光定量PCR内参引物,解决丝瓜实时...选择合适的内参基因是提高实时荧光定量PCR分析(q RT-PCR)准确性的重要条件。18S r RNA基因表达范围广、表达量恒定,常作为内参基因应用于实时荧光定量PCR中。为了获得丝瓜18S r RNA基因,并设计合适的荧光定量PCR内参引物,解决丝瓜实时荧光定量PCR检测中无内参基因的现状,通过PCR和序列测定,首次克隆到了丝瓜的18S r RNA基因序列,其长度为1 862 bp,Gen Bank登录号为KM656452。在此基础上设计1对荧光定量PCR引物,该引物特异性强,扩增效率高,在丝瓜各生长发育阶段及各种非生物胁迫条件下均能稳定表达,适合在丝瓜基因表达研究中作为内参基因。该研究结果可为开展丝瓜重要功能基因的表达模式和调控机制的研究奠定基础。展开更多
基金Suppored by Science Program Foundation of Taizhou City(102TG01)~~
文摘[Objective] The research aimed to understand the genetic control of seed germination characters in dicotyledon plant using a suitable genetic model. [Method] An investigation of three seedling traits of seven parents and their F1s was undertaken in a diallel cross of sponge gourd （Luffa cylindrical Roem.） by germinating the seeds in two environments. A genetic model is developed to analyze the embryo effects as well as genotype × environment （GE） interaction effects of germination characters. [Result] Variations of the root length, shoot length and shoot dry weight were affected by genetic effects and environmental interaction effects. The three seedling traits were mainly controlled by genetic effect. Embryo dominance effects were the main genetic effect for root length and shoot length. Among the GE interaction effects, there only existed embryo dominance interaction effects for the three seedling traits. General narrow-sense heritabilities of shoot length and shoot dry weight were 7.0% and 27.3%, respectively. [Conclusion] The present modified genetic model could be used for investigating the genetic control of seed germination characters and seedling traits in dicotyledon plant.
文摘选择合适的内参基因是提高实时荧光定量PCR分析(q RT-PCR)准确性的重要条件。18S r RNA基因表达范围广、表达量恒定,常作为内参基因应用于实时荧光定量PCR中。为了获得丝瓜18S r RNA基因,并设计合适的荧光定量PCR内参引物,解决丝瓜实时荧光定量PCR检测中无内参基因的现状,通过PCR和序列测定,首次克隆到了丝瓜的18S r RNA基因序列,其长度为1 862 bp,Gen Bank登录号为KM656452。在此基础上设计1对荧光定量PCR引物,该引物特异性强,扩增效率高,在丝瓜各生长发育阶段及各种非生物胁迫条件下均能稳定表达,适合在丝瓜基因表达研究中作为内参基因。该研究结果可为开展丝瓜重要功能基因的表达模式和调控机制的研究奠定基础。
