Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded prote...Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded protein. Methods Nucleus pulposus samples were surgically obtained from 8 patients with degenerat ive lumbar disc disease. The cells were cultured and directly infected by two a denoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence pro tein (EGFP) gene (marker gene) and Ad/CMV-TGF-β_1 containing the potentially therapeutic TGF-β_1 gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-β_1). Results Culture cells transducted by Ad/CMV-EGFP showed specific green fluorescence und er the fluoroscope and expression sustained for at least 4 weeks. When infe cted by Ad/CMV-TGF-β_1, approximally 30% of cultured cells were staind brown (+) with TGF-β_1 staining. Conclusion This study established the strategy of delivering a potentially therapeutic gene , TGF-β_1, by using an adenoviral vector to human degenerative lumbar interve rtebral disc cells.展开更多
12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万...12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万千女性如花绽放的胶原养美新’肌’密。近年来因出演古装穿越剧而人气飙升、荣升新"四小花旦"之首的杨幂,是继浙江卫视当红女主播朱丹之后。展开更多
文摘Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded protein. Methods Nucleus pulposus samples were surgically obtained from 8 patients with degenerat ive lumbar disc disease. The cells were cultured and directly infected by two a denoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence pro tein (EGFP) gene (marker gene) and Ad/CMV-TGF-β_1 containing the potentially therapeutic TGF-β_1 gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-β_1). Results Culture cells transducted by Ad/CMV-EGFP showed specific green fluorescence und er the fluoroscope and expression sustained for at least 4 weeks. When infe cted by Ad/CMV-TGF-β_1, approximally 30% of cultured cells were staind brown (+) with TGF-β_1 staining. Conclusion This study established the strategy of delivering a potentially therapeutic gene , TGF-β_1, by using an adenoviral vector to human degenerative lumbar interve rtebral disc cells.
文摘12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万千女性如花绽放的胶原养美新’肌’密。近年来因出演古装穿越剧而人气飙升、荣升新"四小花旦"之首的杨幂,是继浙江卫视当红女主播朱丹之后。
