To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical po...To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical populations(Beihai,Weizhou Island,Zhanjiang,Xiamen,and Fuzhou)in Guangxi,Guangdong,and Fujian provinces was studied statistically in combination with the microsatellite markers.As revealed by morphological principal component analysis(PCA),the cumulative contribution rate of the first three principal components was 72.596%.The discrimination accuracy ranged from 47.5%to 80.0%,and the scatter plots of principal component and discriminant analysis were consistent in overall,showing that the Xiamen and Fuzhou populations were overlapped obviously.For microsatellite markers,10 pairs of polymorphic primers were obtained by high-throughput transcriptome sequencing,and used for genetic diversity analysis.It was showed that the average number of alleles and eff ective alleles observed in each population ranged from 8.100 to 10.900,and from 3.497 to 4.228,respectively.The average observed heterozygosity(H_(o))and expected heterozygosity(H_(e))in the five populations ranged from 0.541 to 0.615,and from 0.642 to 0.733,respectively.The genetic distance(DA)ranged from 0.078 to 0.523,and the population genetic differentiation index(F_(ST))ranged from 0.027 to 0.139.The unweighted pair-population method with arithmetic means(UPGMA)and structure analysis showed that the five populations could be divided into two main clusters,the Beibu Gulf group(Beihai and Weizhou Island)and the Southeast China Sea group(Zhanjiang,Xiamen,and Fuzhou),suggesting that L.maxima has been separated geographically by the barrier of the Leizhou Peninsula into two groups in evolution,which provided us with a scientific clue to better protect the bioresource and establish an appropriate fishery management stocks for L.maxima populations in south China.展开更多
分别于2008年月12月至2009年3月和2009年5月至8月,2次在北海市乔港镇沿岸浅海进行大獭蛤（Lutraria maxima Jonas）人工苗种自然海区沉箱式中间培育试验。试验培育工具为长方形聚乙烯水果框,规格为长×宽×高=50cm×35cm...分别于2008年月12月至2009年3月和2009年5月至8月,2次在北海市乔港镇沿岸浅海进行大獭蛤（Lutraria maxima Jonas）人工苗种自然海区沉箱式中间培育试验。试验培育工具为长方形聚乙烯水果框,规格为长×宽×高=50cm×35cm×15cm,放苗前框内装满中沙。试验用大獭蛤苗种规格为壳长0.2～0.5cm。分别经过2次75d和45d的中间培育,大獭蛤人工苗种增长至出苗规格2.5～3.5cm,成活率分别为42.56%、41.13%。在海水温度25.0～31.5℃和1000粒/筐养殖密度下,大獭蛤人工苗种的浅海沉箱中间培育生长速度快,成活率高,苗种活力强,养殖成活率高。展开更多
2002年9月至2003年4月,在广西海洋研究所古城基地进行大獭蛤(Lutmria maxima Jonas)苗种池塘中间培育试验。试验在铺沙的池塘里进行,放养的大獭蛤苗种规格为壳长0.2～0.8mm,大獭蛤稚贝饵料为施肥繁殖的浮游藻类,以大部分大獭蛤稚贝壳长...2002年9月至2003年4月,在广西海洋研究所古城基地进行大獭蛤(Lutmria maxima Jonas)苗种池塘中间培育试验。试验在铺沙的池塘里进行,放养的大獭蛤苗种规格为壳长0.2～0.8mm,大獭蛤稚贝饵料为施肥繁殖的浮游藻类,以大部分大獭蛤稚贝壳长达到2cm为出苗标准。结果表明,在水温为20～30℃、盐度为20‰以上,放苗量为375万粒/公顷的条件下,大獭蛤稚贝经过45～60d即可以培育成壳长为2cm左右的大规格苗种。展开更多
基金Supported by the Science and Technology Major Project of Guangxi Province(No.AA17204088)。
文摘To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical populations(Beihai,Weizhou Island,Zhanjiang,Xiamen,and Fuzhou)in Guangxi,Guangdong,and Fujian provinces was studied statistically in combination with the microsatellite markers.As revealed by morphological principal component analysis(PCA),the cumulative contribution rate of the first three principal components was 72.596%.The discrimination accuracy ranged from 47.5%to 80.0%,and the scatter plots of principal component and discriminant analysis were consistent in overall,showing that the Xiamen and Fuzhou populations were overlapped obviously.For microsatellite markers,10 pairs of polymorphic primers were obtained by high-throughput transcriptome sequencing,and used for genetic diversity analysis.It was showed that the average number of alleles and eff ective alleles observed in each population ranged from 8.100 to 10.900,and from 3.497 to 4.228,respectively.The average observed heterozygosity(H_(o))and expected heterozygosity(H_(e))in the five populations ranged from 0.541 to 0.615,and from 0.642 to 0.733,respectively.The genetic distance(DA)ranged from 0.078 to 0.523,and the population genetic differentiation index(F_(ST))ranged from 0.027 to 0.139.The unweighted pair-population method with arithmetic means(UPGMA)and structure analysis showed that the five populations could be divided into two main clusters,the Beibu Gulf group(Beihai and Weizhou Island)and the Southeast China Sea group(Zhanjiang,Xiamen,and Fuzhou),suggesting that L.maxima has been separated geographically by the barrier of the Leizhou Peninsula into two groups in evolution,which provided us with a scientific clue to better protect the bioresource and establish an appropriate fishery management stocks for L.maxima populations in south China.
文摘分别于2008年月12月至2009年3月和2009年5月至8月,2次在北海市乔港镇沿岸浅海进行大獭蛤（Lutraria maxima Jonas）人工苗种自然海区沉箱式中间培育试验。试验培育工具为长方形聚乙烯水果框,规格为长×宽×高=50cm×35cm×15cm,放苗前框内装满中沙。试验用大獭蛤苗种规格为壳长0.2～0.5cm。分别经过2次75d和45d的中间培育,大獭蛤人工苗种增长至出苗规格2.5～3.5cm,成活率分别为42.56%、41.13%。在海水温度25.0～31.5℃和1000粒/筐养殖密度下,大獭蛤人工苗种的浅海沉箱中间培育生长速度快,成活率高,苗种活力强,养殖成活率高。
文摘2002年9月至2003年4月,在广西海洋研究所古城基地进行大獭蛤(Lutmria maxima Jonas)苗种池塘中间培育试验。试验在铺沙的池塘里进行,放养的大獭蛤苗种规格为壳长0.2～0.8mm,大獭蛤稚贝饵料为施肥繁殖的浮游藻类,以大部分大獭蛤稚贝壳长达到2cm为出苗标准。结果表明,在水温为20～30℃、盐度为20‰以上,放苗量为375万粒/公顷的条件下,大獭蛤稚贝经过45～60d即可以培育成壳长为2cm左右的大规格苗种。
