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Chemical constituents from Pithecellobium clypearia and their effects on T lymphocytes proliferation 被引量:13
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作者 郭晓宇 王乃利 +3 位作者 宝丽 李轶华 徐强 姚新生 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第3期208-213,共6页
Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatograp... Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatographic methods and their structures were identified on the basis of spectral analysis. The immufiomodulatory effects of all the compounds were examined by a Con A-induced T lymphocytes proliferation assay. Results Eight compounds were isolated and identified as (-)- epigallocatechin (1), (-)-5, 7, 3′, 4′, 5′-pentahydroxyflavan (2), (-)-epigallocatechin-7-gallate (3), (-)-5, 3′, 4′, 5′-tetrahydroxyfiavan- 7-gallate (4), quercitin-3-O-α-L-rhamnpyranoside (5), myricitin-3-O-α-L-rhamnpyranoside (6), gallic acid (7), and ethyl gallate (8), respectively. Conclusion Compounds 3 and 8 were isolated from this genus for the first time, and compound 1 was isolated from this species for the first time. Compound 3 exhibited a strong inhibition on the T lymphocytes proliferation induced by Con A with an IC50 of 4.4 μmol·L^-1. 展开更多
关键词 Pithecellobium clypearia FLAVONOIDS Flavane lymphocytes proliferation
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Study on Lymphocyte Activation and Proliferation Induced by Anti-CD3 McAb
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作者 李鸣 杨敬 +4 位作者 沈关心 张茜 刘慎沛 刘忠北 叶维新 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1994年第4期209-212,共4页
T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and prolif... T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation. 展开更多
关键词 CD_3 McAb lymphocyte activation and proliferation GTP-binding protein cytoplasmic free calcium
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IMMUNOMODULATION OF SYNTHESIZEDPOLYMERS CONTAINING PHOSPHORUSIN THE BACKBONE──EFFECT ON THE PROLIFERATION OF LYMPHOCYTES
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作者 Zhuo Renxi Wang Jun +2 位作者 Mao Haiquan Zang Chuanbing Ye Ying 《Chinese Journal of Reactive Polymers》 1997年第1期67-76,共10页
The immunomodulation of several charged synthetic polymers containing phosphorus in the backbone was studied in vitro through examining their inhibition or promotion effect on the proliferation of both T and B lymphoc... The immunomodulation of several charged synthetic polymers containing phosphorus in the backbone was studied in vitro through examining their inhibition or promotion effect on the proliferation of both T and B lymphocytes. It is found that polymers based on long chain alkyl ester of tyrosine exhibit immunomodulative activity. Negatively charged polymers show stimulative activiactivity on LPS-induced B lymphocytes proliferation. Positively charged polymers exhibit inhibitory activity on both Con A-induced T lymphocytes and LPS-induced B lymphocytes proliferation. 展开更多
关键词 Phosphorus-containing polymer lymphocytes proliferation IMMUNOMODULATION
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Effect of IL-10 gene transmission on the PTg-stimulated splenocytes proliferation and Th1 cytokines production from experimental autoimminue thyroiditis rats 被引量:1
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作者 Wei Tang Cuiping Liu Youwen Qin Yue Jia XiaodongMao Chao Liu 《Journal of Nanjing Medical University》 2007年第1期55-58,共4页
To investigate the effects of gene therapy with IL-10 on PTg-induced proliferation of splenocytes and Thl cytokine production from PTg-stimulated splenocytes. Methods: EAT rats were divided into four groups :group A... To investigate the effects of gene therapy with IL-10 on PTg-induced proliferation of splenocytes and Thl cytokine production from PTg-stimulated splenocytes. Methods: EAT rats were divided into four groups :group A (PBS+PLL) , group B (pORF+PLL), group C (pORFmIL10+PLL), and group D (pORFmIL10+ MEM). The substances mixed with lipofectamine were injected into the thyroid tissues of rats on the 18th dday after immunization. The rats were sacrificed at the 8th week. In vitro proliferative responses to ConA and different concentration of PTg were measured by culturing 4 ×105 splenocytes pulsed with 18.5KBq of [^3H] thymidine for the final 12h and then harvested for liquid scintillation counting. In vitro splenocytes were cultured with PTg (25 mg/L). Thl cytokine IFN-γ,TNF-α and IL-2 were detected by ELISA. Results: The proliferative response to PTg was suppressed in group C, compared with that of group A and B (P 〈 0.05). The levels of IFN-γ,TNF-αand IL-2 in the supernatant of PTg-stimulated splenocytes were 3548.25±779.47 pg/ml, 27.66±10.50 pg/ml and 3617.73±609.15 pg/ml, respectively, which were much lower in group C than those in group A and B(P 〈 0.01, P 〈 0.05, P 〈 0.001, respectively). Conclusion: IL- 10 gene transmission in thyroid tissues could inhibit PTg specific proliferation of splenocytes from EAT rats and the secretion 6f Th1 cytokines from PTg-stimulated splenocytes. 展开更多
关键词 INTERLEUKIN-10 autoimmune thyroiditis lymphocytic proliferation AUTOIMMUNITY
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Effects of Tremella Polysaccharide on Immune Function of Physically-Stressed Mice 被引量:3
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作者 崔金莺 林志彬 《Journal of Chinese Pharmaceutical Sciences》 CAS 1992年第2期49-53,共5页
An immunosuppressive animal model induced by physical stress that forced mice to swim in cold water(14±1℃)and the restorative effect of Tremella polysac charide(TP)on the suppressed immune function by stress wer... An immunosuppressive animal model induced by physical stress that forced mice to swim in cold water(14±1℃)and the restorative effect of Tremella polysac charide(TP)on the suppressed immune function by stress were studied in mice.It was found that the spleen plaque forming cell(PFC)response to sheep red blood cells,delayed cuta- neous hypersensitivity(DCH)induced by dinitrochlorobenzene and the lymphocyte prolifer- ation stimulated by concanavalin A(Con A)were significantly decreased in stressed mice. In addition.the maximal decrease of PFC was reached in 9-12 days after stress.A- drenolectomy could not affect the decrease of PFC in stressed mice.TP(200.400mg/kg) ig for 8-14 days significantly restored the PFC.DCH and lymphocyte proliferation to nor- mal level in stressed mice. 展开更多
关键词 Tremella polysaccharide Physical stress Plaque forming cell (PFC) Delayed cutaneous hypersensitivity (DCH) lymphocyte proliferation
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Enhancement of the Immune Response to Rabbit Hemorrhagic Disease Vaccine in Young Rabbits by Advanced Vaccination and Chinese Herbal Adjuvants 被引量:2
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作者 YANG Long-sheng XUE Jia-bin +3 位作者 HU Yuan-liang WANG Fang WANG De-yun XU Wei-zhong 《Agricultural Sciences in China》 CAS CSCD 2008年第10期1274-1280,共7页
Experiments were conducted to determine the effects of advanced vaccination and Chinese herbal adjuvants (CHA), containing astragalus polysaccharides (APS) and ginsenosides (GS) on the immune response to rabbit ... Experiments were conducted to determine the effects of advanced vaccination and Chinese herbal adjuvants (CHA), containing astragalus polysaccharides (APS) and ginsenosides (GS) on the immune response to rabbit hemorrhagic disease (RHD) vaccine in young rabbits. In experiment 1, 5 New Zealand rabbits of each group at 30, 35, 40, or 45 days of age were injected with 2 mL of inactivated RHD vaccine, respectively. The dynamic changes of antibody titers were tested by the hemagglutination inhibition (HI) method. In experiment 2, 30 New Zealand rabbits at 35 days of age were randomly assigned to 5 treatment groups, representing inoculation with 3 mL of non-adjuvant RHD vaccine, CHA-RHD vaccine, CHA-HA vaccine (half dose antigen), aluminium adjuvant-RHD vaccine, and PBS, respectively. The dynamic changes of peripheral lymphocyte proliferation and serum antibody titers were tested by the MTT method and the HI method. The results showed that the titer of maternal HI antibody in the 35-day-old rabbits was lower than the protective level of 3 log2, while on days 7 to 49 after the vaccination, the antibody titers were higher than 3 log2. The CHA promoted the lymphocyte proliferation and enhanced the serum antibody titer (P〈 0.05). These findings from the two experiments suggested that advanced vaccination and Chinese herbal adjuvants significantly enhanced the immune response to vaccine against RHD, and effectively protected the young rabbits against RHD challenge. 展开更多
关键词 rabbit hemorrhagic disease Chinese herbal adjuvants antibody titer lymphocyte proliferation
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Effects of methyl-,phenyl-,ethylmercury and mercurychlorid on immune cells of harbor seals(Phoca vitulina)
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作者 Antje Kakuschke Elizabeth Valentine-Thon +2 位作者 Sonja Fonfara Katharina Kramer Andreas Prange 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第12期1716-1721,共6页
Mercury (Hg) is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the infl... Mercury (Hg) is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the influence of methyl-, ethyl- and phenylmercury as well as mercurychloride on immune functions was evaluated. Two parameters of cellular immunity, proliferation and mRNA cytokine expression of interleukin-2, -4, and transforming growth factor β, were investigated in harbor seal lymphocytes after in vitro exposure to Hg compounds. While all Hg compounds had a suppressive effect on proliferation, differences between juvenile and adult seals were found. Lymphocytes from juveniles showed a higher susceptibility to the toxic effect compared to lymphocytes from adults. Furthermore, the degree of inhibition of proliferation varied among the four Hg compounds. The organic compounds seem to be more immunotoxic than the inorganic compound. Finally, for the cytokine expression of methylmercury-incubated lymphocytes, timedependent changes were observed, but no dose-dependency was found. Marine mammals of the North Sea are burdened with Hg, and lymphocytes of harbor seals may be functionally impaired by this metal. The present in vitro study provides baseline information for future studies on the immunotoxic effects of Hg on cellular immunity of harbor seals. 展开更多
关键词 harbor seal MERCURY lymphocyte proliferation cytokine expression North Sea
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Immunostimulatory Properties of a Novel Polyphosphoramidate Based on Stearyl Tyrosine
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作者 Zhuo Renxi Wang Jun Mao Haiquan 《Wuhan University Journal of Natural Sciences》 CAS 1997年第2期69+71+73-74,70+72,共6页
The immunostimulatory property of a synthetic polyphosphoramidate based on stearyl tyrosine (PPST) is described. The immunostimulatory activity of this negatively charged polymer was investigated in vitro through ... The immunostimulatory property of a synthetic polyphosphoramidate based on stearyl tyrosine (PPST) is described. The immunostimulatory activity of this negatively charged polymer was investigated in vitro through examining the effect on the mitogen induced lymphocytes proliferation by a tetrazolium based colormetric assay and also through studying the primary plaque forming cells (IgM PFC) responses of Balb/c mice after the intraperitoneal (i.p.) injection of the polymer with sheep red blood cells (SRBC). PPST exhibits specific and high immunostimulatory activity in vitro by the stimulation on lipopolysaccharide(LPS) induced murine B cells proliferation, which is probably due to the interaction between the polymer with LPS responding B lymphocytes. This polymer also makes a significant increase of single antibody producing cells. The result shows that the stimulation rate to IgM PFC responses of Balb/c mice achieved 77.9% ( p <0.01) when 160 mg/kg of PPST was injected 4 h before with SRBC. 展开更多
关键词 polyphosphoramidate stearyl tyrosine IMMUNOSTIMULANT lymphocytes proliferation
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Effects of purified zearalenone on selected immunological and histopathologic measurements of spleen in post-weanling gilts 被引量:6
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作者 Peng Chen Tingjun Liu +3 位作者 Shuzhen Jiang Zaibin Yang Libo Huang Faxiao Liu 《Animal Nutrition》 SCIE 2017年第3期212-218,共7页
The present study was aimed at investigating the adverse effects of dietary zearalenone(ZEA) on the lymphocyte proliferation rate(LPR), interleukin-2(IL-2), mRNA expressions of pro-inflammatory cytokines, and histopat... The present study was aimed at investigating the adverse effects of dietary zearalenone(ZEA) on the lymphocyte proliferation rate(LPR), interleukin-2(IL-2), mRNA expressions of pro-inflammatory cytokines, and histopathologic changes of spleen in post-weanling gilts. A total of 20 crossbred piglets(Yorkshire × Landrace × Duroc) with an initial BW of 10.36 ± 1.21 kg(21 d of age) were used in the study.Piglets were fed a basal diet with an addition of 0.1.1,2.0, or 3.2 mg/kg purified ZEA for 18 d ad libitum.The results showed that LPR and IL-2 production of spleen decreased linearly(P < 0.05) as dietary ZEA increased. Splenic mRNA expressions of interleukin-1β(IL-1β) and interleukin-6(IL-6) were linearly upregulated(P < 0.05) as dietary ZEA increased. On the contrary, linear down-regulation(P<0.05) of mRNA expression of interferon-γ(IFN-γ) was observed as dietary ZEA increased. Swelling splenocyte in1.1 mg/kg ZEA treatments, atrophy of white pulp and swelling of red pulp in 2.0 and 3.2 mg/kg ZEA treatments were observed. The cytoplasmic edema in 1.1 mg/kg ZEA treatments, significant chromatin deformation in 2.0 mg/kg ZEA treatment and phagocytosis in 3.2 mg/kg ZEA treatment were observed.Results suggested that dietary ZEA at 1.1 to 3.2 mg/kg can induce splenic damages and negatively affect immune function of spleen in post-weanling gilts. 展开更多
关键词 Pro-inflammatory cytokines GILT HISTOPATHOLOGY IL-2 lymphocyte proliferation rate ZEARALENONE
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Clinical Study on the Effect of Electroacupuncture on Cellular Immune Function in Patients with Gastrointestinal Tumor 被引量:5
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作者 汪军 姜建伟 +5 位作者 蔡三军 彭惠婷 高艳琴 曹小定 吴根诚 韩丑萍 《Journal of Acupuncture and Tuina Science》 2011年第6期354-358,共5页
Objective: To investigate the effect of electroacupuncture (EA) on the T-lymphocyte subsets and lymphocyte transformation in patients with gastrointestinal tumor during peri-operative and peri-chemotherapy periods.... Objective: To investigate the effect of electroacupuncture (EA) on the T-lymphocyte subsets and lymphocyte transformation in patients with gastrointestinal tumor during peri-operative and peri-chemotherapy periods. Methods: A total of 44 cases with gastrointestinal tumor were allocated into 4 groups of surgical removal (SR), SR + electroacupuncture (EA), SR + chemotherapy, and SR + chemotherapy + EA. Then changes of T-lymphocyte subsets in varying stages of gastrointestinal tumor were determined using a flow cytometer. Results: On the 3rd day after operation, the patients' CD3+ cell percentage and lymphocyte transformation were significantly lower than that before operation (P〈0.05, P〈0.01); the CD3+ cell percentage and lymphocyte transformation of patients who received EA were not significantly reduced, showing a statistic difference when compared with the SR group (P〈0.05). On the 10th day after operation, the patients' lymphocyte transformation was almost restored to the level before operation, the CD8+ cells in patients who received EA were significantly reduced (P〈0.05) and their CD4+/CD8+ ratios were markedly elevated (P〈0.05). On the 3rd day after operation coupled with chemotherapy, the CD3+ cell percentage and CD4+/CD8+ ratios were somewhat reduced, but not showing a statistic difference. Upon EA intervention, the CD3+ cell percentage and CD4+/CD8+ ratios on the third day after operation coupled with chemotherapy were significantly increased (P〈0.05). The intra-group comparison before and after treatment has shown that EA can improve the lymphocyte transformation of patients after operation and chemotherapy (P〈0.01). Conclusion: EA can substantially improve the cellular immune function in patients with gastrointestinal tumor during peri-operative and peri-chemotherapy periods. 展开更多
关键词 Electroacuouncture: Gastrointestinal Tumor T Lvmohocvte Subsets lymphocyte proliferation
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Effects and mechanisms of Cryptotanshinone on rats with adjuvant arthritis 被引量:1
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作者 ZHENG Fu-lin CHANG Yan +2 位作者 JIA Xiao-yi HUANG Min WEI Wei 《Chinese Medical Journal》 SCIE CAS CSCD 2011年第24期4293-4298,共6页
Background Cryptotanshinone (CT) is the major active constituent of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of CT on rats with adjuvant arthritis (AA). Methods AA wa... Background Cryptotanshinone (CT) is the major active constituent of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of CT on rats with adjuvant arthritis (AA). Methods AA was induced by the metatarsal footpad injection with complete Freund's adjuvant in male Sprague-Dawley rats. The secondary inflammatory reaction was evaluated by hind paw swelling and the polyarthritis index. Activity of interleukin-1 (IL-1) was detected by the concanavalin A-induced thymocytes proliferation assay. The lymphocytes proliferation and IL-2 production were assayed by 3-(4,5-2dimethylthiazal-2yl)2,5-diphenyltetrazoliumbromide (MTT) and activated mouse splenocytes proliferation, respectively. Results Intragastric administration of CT (50 and 100 mg/kg) significantly decreased secondary inflammatory reactions and increased the spleen and thymus index. There was a marked immunologic and inflammatory response in the AA model, which was accompanied by the decrease of thymocyte proliferation and IL-2 production as well as the increase of IL-1 production. CT apparently enhanced thymocyte proliferation and decreased IL-1 production in AA rats. Conclusion These results indicate that CT may exert its anti-inflammatory and immunoregulatory effects through inhibiting lymphocyte proliferation and production of pro-inflammatory mediators. 展开更多
关键词 CRYPTOTANSHINONE adjuvant arthritis lymphocyte proliferation CYTOKINE
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Immunotoxic effects of an industrial waste incineration site on groundwater in rainbow trout (Oncorhynchus mykiss)
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作者 Nadjet Benchalgo Franois Gagn Michel Fournier 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2014年第5期981-990,共10页
The discharge of organic waste from the petrochemical industry into the Mercier lagoons caused major groundwater contamination. The objective of this study was to determine the immunotoxic potential of three groundwat... The discharge of organic waste from the petrochemical industry into the Mercier lagoons caused major groundwater contamination. The objective of this study was to determine the immunotoxic potential of three groundwater wells at increasing distance from the incinerator dumping site (1.17, 2.74 and 5.40 km). Rainbow Trout were exposed to increasing concentrations of water from three groundwater wells for 14 days. Immunocompetence was characterized by phagocytosis, mitogen-stimulated proliferation of lymphocytes, cell cycle analysis and apoptosis. A significant increase in innate (phagocytosis) and specific immune response (B lymphocyte proliferation) was observed in trout exposed to water collected from the well at 2.74 km. However, phagocytosis activity was suppressed in groups at 1.17 and 5.40 km. The proportion of lymphocytes in S phase was significantly increased in groups at 2.74 and 5.40 kin, while lymphocytes in G0/G1 phase were decreased in all three exposure groups. Additionally, dexamethasone (DEX)-induced apoptosis of lymphocytes was significantly reduced in the group at 2.74 km, which suggests decreased lymphocyte turnover. Furthermore, the ratio of DEX- induced apoptosis/apoptosis was lower in the groups at 2.74 and 5.40 km. In summary, our experiments have shown that exposure to the mixture of organic compounds present in Mercier groundwater modulates phagocytosis and cell proliferation, disrupts the cell cycle and reduces the ratio of DEX- induced apoptosis/apoptosis. It is concluded that groundwater collected in the vicinity of an incinerator containment field could impact immunocompetence in fish. 展开更多
关键词 GROUNDWATER PHAGOCYTOSIS lymphocyte proliferation cell cycle apoptosis rainbow trout
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Cell-wall-deficient bacteria: a major etiological factor for psoriasis? 被引量:1
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作者 WANG Guo-li LI Xiu-yun +5 位作者 WANG Ming-yi XIAO De-gui ZHANG Yong-yu YUAN Xiao-yan WANG Qi-you SONG Jian-jing 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第24期3011-3016,共6页
Background Psoriasis is a common inflammatory skin disease, yet knowledge of the factors that may induce, trigger, or exacerbate psoriasis is not fully delineated. Recent advances have improved our understanding of th... Background Psoriasis is a common inflammatory skin disease, yet knowledge of the factors that may induce, trigger, or exacerbate psoriasis is not fully delineated. Recent advances have improved our understanding of the link between psoriasis and cell-wall-deficient bacteria (CWDB) infections. In the present study we assessed the prevalence of CWDB infection in patients with psoriasis. Methods The carriage rate of CWDB in the tonsil or pharynx of psoriasis patients, chronic tonsillitis patients and controls were investigated using hypertonic medium. Psoriasis patients with CWDB were randomly assigned to two groups and respectively treated with antibiotics or systemic therapy without antibiotic. Human peripheral blood mononuclear cells (PBMC) from psoriasis patients, chronic tonsillitis patients and control subjects were stimulated with bacteria antigens and extra-cellular levels of interferon-γ(IFN-γ) and interleukin (IL)-10 were measured in the supernatants using the ELISA technique, in vitro. Meanwhile, the proliferation ability of PBMC to respond to bacteria antigens was detected by MTT assay. Results CWDB were isolated from 74.2% of psoriasis patients, 23.5% of chronic tonsillitis patients and only 6.3% of controls. Antibiotic therapy was appropriate for approximately 80% of psoriasis patients with CWDB infection, and in only 8.9% psoriasis patients CWDB infection was detected after antibiotic therapy. Meanwhile, our study showed that CWDB and wide-type bacteria did remarkably enhance the production of IFN-γ, in vitro, and PBMC proliferation. Conclusion CWDB infection may be a virtual triggering factor in psoriasis by regulating T-cell activation. 展开更多
关键词 cell-wall-deficient bacteria PSORIASIS INTERFERON-Γ T lymphocytes proliferation antibiotic therapy
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