In a recent single cell transcriptomic investigation of the peripheral immune responses of the patients with severe COVID-19[1],no substantial expression of pro-inflammatory cytokines has been identified in the periph...In a recent single cell transcriptomic investigation of the peripheral immune responses of the patients with severe COVID-19[1],no substantial expression of pro-inflammatory cytokines has been identified in the peripheral monocytes and lymphocytes,while these peripheral immune cells exhibit phenotypes of heterogeneously expressed interferon-stimulated genes in certain COVID-19 patients.These genes may contribute to the inhibitory response to the viral entry,translation,replication and egress.Questions remain regarding why the peripheral cytokine production is constrained,what additional roles these peripheral immune cells may play in COVID-19 pathophysiology,and which potential targets can be explored for facilitating COVID-19 therapeutics.展开更多
In the work, we offer a brief report by research results demonstrating correlations between energy status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing on our preliminary d...In the work, we offer a brief report by research results demonstrating correlations between energy status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing on our preliminary data, showing correlations between succinate dehydrogenase (SDH) activity in peripheral blood lymphocytes and speed or duration of pyridine nucleotides reduction in mitochondria of rat brain and liver tissues, we propose an idea of using cytobiochemical method of SDH estimation in peripheral blood lymphocytes for evaluation of tissue mitochondria status. The method could be successfully used in clinical practice for early revealing of energy metabolism disturbances that allows preventing disease development and optimizing the diseases therapy.展开更多
BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is one of the main chronic liver diseases.However,the roles of mitochondrial carnitine palmitoyl transferase-II(CPT-II)downregulation and liver can...BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is one of the main chronic liver diseases.However,the roles of mitochondrial carnitine palmitoyl transferase-II(CPT-II)downregulation and liver cancer stem cell(LCSC)activation remain to be identified.AIM To investigate the dynamic alterations in CPT-II inactivity and LCSC activation during the malignant progression of MAFLD.METHODS Dynamic models of mouse MAFLD were generated via the consumption of a high-fat diet or the addition of 2-fluorenylacetamide for hepatocarcinogenesis.The mice were divided into groups on the basis of hematoxylin and eosin staining.Biochemistries,CPT-II,intrahepatic T cells,and LCSCs were determined and confirmed in clinical samples.The mitochondrial membrane potential(MMP)was analyzed.Differentially expressed genes were screened via RNA sequencing and enriched in KEGG pathways or GO functions.RESULTS Dynamic models of MAFLD malignant transformation were successfully generated on the basis of pathological examination.Hepatic lipid accumulation was associated with the loss of mitochondrial CPT-II activity and alterations in the MMP,with decreases in liver CD3+or CD4+T cells and increased AFP levels.In the lipid accumulation microenvironment,mitochondrial CPT-II was inactivated,followed by aberrant activation of CD44+or CD24+LCSCs,as validated in MAFLD or hepatocellular carcinoma patient samples.In terms of mechanism,the biological process category focused mainly on the metabolic regulation of cells in response to external stimuli.The enriched molecular functions included protein binding,cell apoptosis,and cell proliferation.CONCLUSION CPT-II inactivity promotes the malignant progression of MAFLD via the loss of innate immune function and abnormal LCSC activation.展开更多
Energy homeostasis is essential for maintaining cell survival and regular functions, and the loss of energy homeostasis may result in a variety of diseases. In this review, we focus on the roles and regulatory mechani...Energy homeostasis is essential for maintaining cell survival and regular functions, and the loss of energy homeostasis may result in a variety of diseases. In this review, we focus on the roles and regulatory mechanisms of T lymphocytes energy metabolism in its activation, differentiation and physiological function. We also discuss the significance of T cell energy metabolism for the treatment of inflammatory diseases.展开更多
The architecture feature of spleen white pulp of the Chinese soft-shelled turtle,Pelodiseus Sinensis were examined in detail by the microscopy and electron microscopy.The results showed that the ellipsoid capillary wa...The architecture feature of spleen white pulp of the Chinese soft-shelled turtle,Pelodiseus Sinensis were examined in detail by the microscopy and electron microscopy.The results showed that the ellipsoid capillary was different from capillary in the other kinds of animals,it belonged to high endothelial capillary.The endothelial cells of the ellipsoid capillary were cube or cylindroid.So,it展开更多
基金support received from the Scientific Research Grant of Ningbo University(215-432000282)Ningbo Top Talent Project(215-432094250).
文摘In a recent single cell transcriptomic investigation of the peripheral immune responses of the patients with severe COVID-19[1],no substantial expression of pro-inflammatory cytokines has been identified in the peripheral monocytes and lymphocytes,while these peripheral immune cells exhibit phenotypes of heterogeneously expressed interferon-stimulated genes in certain COVID-19 patients.These genes may contribute to the inhibitory response to the viral entry,translation,replication and egress.Questions remain regarding why the peripheral cytokine production is constrained,what additional roles these peripheral immune cells may play in COVID-19 pathophysiology,and which potential targets can be explored for facilitating COVID-19 therapeutics.
文摘In the work, we offer a brief report by research results demonstrating correlations between energy status of peripheral blood lymphocytes and functional state of vital tissues mitochondria. Basing on our preliminary data, showing correlations between succinate dehydrogenase (SDH) activity in peripheral blood lymphocytes and speed or duration of pyridine nucleotides reduction in mitochondria of rat brain and liver tissues, we propose an idea of using cytobiochemical method of SDH estimation in peripheral blood lymphocytes for evaluation of tissue mitochondria status. The method could be successfully used in clinical practice for early revealing of energy metabolism disturbances that allows preventing disease development and optimizing the diseases therapy.
基金Supported by the National Natural Science Foundation of China,No.32470985 and No.81673241.
文摘BACKGROUND Metabolic dysfunction-associated fatty liver disease(MAFLD)is one of the main chronic liver diseases.However,the roles of mitochondrial carnitine palmitoyl transferase-II(CPT-II)downregulation and liver cancer stem cell(LCSC)activation remain to be identified.AIM To investigate the dynamic alterations in CPT-II inactivity and LCSC activation during the malignant progression of MAFLD.METHODS Dynamic models of mouse MAFLD were generated via the consumption of a high-fat diet or the addition of 2-fluorenylacetamide for hepatocarcinogenesis.The mice were divided into groups on the basis of hematoxylin and eosin staining.Biochemistries,CPT-II,intrahepatic T cells,and LCSCs were determined and confirmed in clinical samples.The mitochondrial membrane potential(MMP)was analyzed.Differentially expressed genes were screened via RNA sequencing and enriched in KEGG pathways or GO functions.RESULTS Dynamic models of MAFLD malignant transformation were successfully generated on the basis of pathological examination.Hepatic lipid accumulation was associated with the loss of mitochondrial CPT-II activity and alterations in the MMP,with decreases in liver CD3+or CD4+T cells and increased AFP levels.In the lipid accumulation microenvironment,mitochondrial CPT-II was inactivated,followed by aberrant activation of CD44+or CD24+LCSCs,as validated in MAFLD or hepatocellular carcinoma patient samples.In terms of mechanism,the biological process category focused mainly on the metabolic regulation of cells in response to external stimuli.The enriched molecular functions included protein binding,cell apoptosis,and cell proliferation.CONCLUSION CPT-II inactivity promotes the malignant progression of MAFLD via the loss of innate immune function and abnormal LCSC activation.
基金supported by the National Natural Science Foundation of China(91439206,31230035,81370006)
文摘Energy homeostasis is essential for maintaining cell survival and regular functions, and the loss of energy homeostasis may result in a variety of diseases. In this review, we focus on the roles and regulatory mechanisms of T lymphocytes energy metabolism in its activation, differentiation and physiological function. We also discuss the significance of T cell energy metabolism for the treatment of inflammatory diseases.
文摘The architecture feature of spleen white pulp of the Chinese soft-shelled turtle,Pelodiseus Sinensis were examined in detail by the microscopy and electron microscopy.The results showed that the ellipsoid capillary was different from capillary in the other kinds of animals,it belonged to high endothelial capillary.The endothelial cells of the ellipsoid capillary were cube or cylindroid.So,it
