Experimental study both in vitro and in vivotogether with clinical trials showed that LAKcells have antitumor and antimetastatic effects(1-5)and that these effects are closely related tothe number of LAK cells transfe...Experimental study both in vitro and in vivotogether with clinical trials showed that LAKcells have antitumor and antimetastatic effects(1-5)and that these effects are closely related tothe number of LAK cells transferred and the ad-ministration of rIL-2(1,6-8).Usually,autologousPBL’s are used as the source of LAK precursorsin the adoptive immunotherapy of cancer patients.But this not only puts an added burden on thecancer patient,it can cause serious side effectsas well(9).Although TIL’s may provide a solu-tion to this problem(10,11),their isolation fromsolid tumors is complex and consumes many rea-gents.We have reported that the isolation oflymphocytes from malignant ascites or from ma-lignant pleural effusions is not only simple展开更多
Morphological changes of lymphokine activated killer (LAK) cells and human rectal adenocarcinoma (HR8348) cells were observed under scanning electron microscope (SEM) during the interaction of the two kinds of the cel...Morphological changes of lymphokine activated killer (LAK) cells and human rectal adenocarcinoma (HR8348) cells were observed under scanning electron microscope (SEM) during the interaction of the two kinds of the cells in vitro. It was showed that the effector and target cells could actively and chemotactically move toward each other. LAK and HR8348 cells contacted with each other through cellular projections and microvilli. The LAK cells simply attached to the HR8348 cells at the early stagr, followed by jigsaw-like binding with them. After conjunction, the target cells' membrane appeared to blister and became perforated. The conjunction of microvilli and projection may act as a physical microbridge in the lysis of the target cells, and killing factors may mediate the lysis by the microbridge. 'Closed chamber' was formed between the conjunctive microvilli, which may play an important role in maintaining local concentration of the killing substances.展开更多
Adoptive immunotherapy, the therapeutic infusion of ex vivo activated cancer-fighting white blood cells that was pioneered by Dr. Steven Rosenberg over 30 years ago, has become more widespread due to outstanding publi...Adoptive immunotherapy, the therapeutic infusion of ex vivo activated cancer-fighting white blood cells that was pioneered by Dr. Steven Rosenberg over 30 years ago, has become more widespread due to outstanding published research documenting the clinical efficacy of this strategy. Based on the well-established in vivo functions of NK and NKT cells, their integral role in the efficacy of certain chemotherapeutic and immunomodulatory agents, and their direct therapeutic action as displayed in clinical trials, the use of autologous natural killer cell infusions is an appropriate and warranted therapeutic option for the treatment of malignant diseases, especially in patients whose disease is refractory to standard treatments such as chemotherapy and radiation.展开更多
文摘Experimental study both in vitro and in vivotogether with clinical trials showed that LAKcells have antitumor and antimetastatic effects(1-5)and that these effects are closely related tothe number of LAK cells transferred and the ad-ministration of rIL-2(1,6-8).Usually,autologousPBL’s are used as the source of LAK precursorsin the adoptive immunotherapy of cancer patients.But this not only puts an added burden on thecancer patient,it can cause serious side effectsas well(9).Although TIL’s may provide a solu-tion to this problem(10,11),their isolation fromsolid tumors is complex and consumes many rea-gents.We have reported that the isolation oflymphocytes from malignant ascites or from ma-lignant pleural effusions is not only simple
文摘Morphological changes of lymphokine activated killer (LAK) cells and human rectal adenocarcinoma (HR8348) cells were observed under scanning electron microscope (SEM) during the interaction of the two kinds of the cells in vitro. It was showed that the effector and target cells could actively and chemotactically move toward each other. LAK and HR8348 cells contacted with each other through cellular projections and microvilli. The LAK cells simply attached to the HR8348 cells at the early stagr, followed by jigsaw-like binding with them. After conjunction, the target cells' membrane appeared to blister and became perforated. The conjunction of microvilli and projection may act as a physical microbridge in the lysis of the target cells, and killing factors may mediate the lysis by the microbridge. 'Closed chamber' was formed between the conjunctive microvilli, which may play an important role in maintaining local concentration of the killing substances.
文摘Adoptive immunotherapy, the therapeutic infusion of ex vivo activated cancer-fighting white blood cells that was pioneered by Dr. Steven Rosenberg over 30 years ago, has become more widespread due to outstanding published research documenting the clinical efficacy of this strategy. Based on the well-established in vivo functions of NK and NKT cells, their integral role in the efficacy of certain chemotherapeutic and immunomodulatory agents, and their direct therapeutic action as displayed in clinical trials, the use of autologous natural killer cell infusions is an appropriate and warranted therapeutic option for the treatment of malignant diseases, especially in patients whose disease is refractory to standard treatments such as chemotherapy and radiation.
