[ Objective ] The paper was to study the occurrence regularity of Lysiphlebus testaceipes Cresson. [ Method ] With Aphis craccivora Koch as the breeding host, developmental duration, development threshold temperature ...[ Objective ] The paper was to study the occurrence regularity of Lysiphlebus testaceipes Cresson. [ Method ] With Aphis craccivora Koch as the breeding host, developmental duration, development threshold temperature and effective accumulative temperatme of L. testaceipes Cresson were studied at six constant tem- peratures of 12, 16, 20, 24, 28 and 32℃ in the laboratory, respectively. [ Result] The developmental durations of each growth stage of L. testaceipes Cresson were shortened with the increasing temperature ranged from 12 to 32℃, and the growth rate was significantly accelerated with the increasing temperature. However, when the temperature was up to 32℃, the growth and development from mummy to adult was suppressed, and the developmental duration was extended for 0.45 d than that at 28℃. The developmental durations from egg to adult at 12, 16, 20, 24, 28 and 32℃ were 38.50, 21.25, 14.11, 12.17, 10.28 and 9.01 d, respectively. The developmental threshold temperatures of egg-mummy, mummy-adult and egg-adult were estimated to be 6.5, 6.25 and 5.36℃, respectively; and the effective accumulated temperatures were 136.28, 75.74 and 227.23 d·℃, respectively. [ Conclusion] Forecast formula of developmental duration and Logistic models were established according to effective accumulated temperature and developmental threshold temperature.展开更多
文摘【目的】本研究旨在从蛋白质组整体层面阐明茶足柄瘤蚜茧蜂Lysiphlebus testaceipes蛹滞育背后的多蛋白调控,重点筛选与能量代谢相关的滞育关联蛋白并分析其功能,有助于更好地理解茶足柄瘤蚜茧蜂蛹滞育的代谢机制。【方法】利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantification,iTRAQ)技术比较了茶足柄瘤蚜茧蜂滞育蛹与非滞育蛹的蛋白含量;利用GO,KEGG网络数据库等生物信息学方法分析鉴定茶足柄瘤蚜茧蜂滞育蛹与非滞育蛹中差异表达蛋白(differentially expressed proteins,DEPs)。【结果】分析得到茶足柄瘤蚜茧蜂滞蛹与非滞育蛹DEPs有135个,包括滞育蛹中上调表达蛋白有38个,下调表达蛋白有97个。GO和KEGG富集分析表明,与天冬氨酸转运、L-谷氨酸转运、胆碱脱氢酶活性和胆碱生物合成甘氨酸甜菜碱条目以及氧化磷酸化通路相关的蛋白上调表达。【结论】氧化磷酸化通路相关蛋白在茶足柄瘤蚜茧蜂滞育过程中呈显著上调表达,说明能量代谢与该蜂滞育密切相关,并推测氧化磷酸化过程除为滞育蛹提供维持生命基本活动的主要能量外,还提供热能。
基金National Key R&D Project"Prevention and Control Techniques and Product Development of Natural Enemies"(SQ2017ZY060059)
文摘[ Objective ] The paper was to study the occurrence regularity of Lysiphlebus testaceipes Cresson. [ Method ] With Aphis craccivora Koch as the breeding host, developmental duration, development threshold temperature and effective accumulative temperatme of L. testaceipes Cresson were studied at six constant tem- peratures of 12, 16, 20, 24, 28 and 32℃ in the laboratory, respectively. [ Result] The developmental durations of each growth stage of L. testaceipes Cresson were shortened with the increasing temperature ranged from 12 to 32℃, and the growth rate was significantly accelerated with the increasing temperature. However, when the temperature was up to 32℃, the growth and development from mummy to adult was suppressed, and the developmental duration was extended for 0.45 d than that at 28℃. The developmental durations from egg to adult at 12, 16, 20, 24, 28 and 32℃ were 38.50, 21.25, 14.11, 12.17, 10.28 and 9.01 d, respectively. The developmental threshold temperatures of egg-mummy, mummy-adult and egg-adult were estimated to be 6.5, 6.25 and 5.36℃, respectively; and the effective accumulated temperatures were 136.28, 75.74 and 227.23 d·℃, respectively. [ Conclusion] Forecast formula of developmental duration and Logistic models were established according to effective accumulated temperature and developmental threshold temperature.
