The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of ...The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of the coagulation bath and coagulating time on the absorptive ability of alginate fiber were discussed. The results show that the absorptive ability to different substances varies in such order： physiological saline 〉 blood 〉 tap water 〉distilled water. The absorptive ability of the alginate fiber increases with the increase of M/G value and the concentration of sodium-alginate. Gradually increasing the concntration of coagulation bath, it increases until the concentration is up to 5%. However, it reduces with lasting coagulating tine.展开更多
By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and ...By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and 26 cases of hepatocellular carcinoma (HCC) patient. It was found that the upper limits of normal male and female were 1.1 and 1.4 ng/ml (expressed as M2-PyK) respectively. The plasma M-PyK in HCC patients was significant increased to above 5 times of average normal level, the positive rate was about 95%. In 6 cases of subclinical small hepatocarcinoma and 7 cases of HCC patient with normal serum alpha-fetal protein level, the mean plasma M-PyK value was also increased. Whereas the plasma M-PyK level in acute or chronic hepatitis and other benign diseases were normal. After the HCC being resected, the plasma M-PyK returned to normal, but increased again in the cases of recurrent hepatocarcinoma, suggesting that the increased M-PyK in the plasma of HCC patient was criginated from M2-type PyK in HCC tissue. Therefore, plasma M-PyK may become a new micro-level index of hepatocarcinoma.展开更多
基金The Research Fund for the Doctoral Program of Higher Education(No. 20061065002)The Research on Science and Technology Item of Shandong Province (No. 2006GG3205005)
文摘The M/G values of different sodium alginate were studied by means of H-NMR. The alginate fiber was prepared via wet-spinning technology. The effects of M/G, the concentration of sodium-alginate, the concentration of the coagulation bath and coagulating time on the absorptive ability of alginate fiber were discussed. The results show that the absorptive ability to different substances varies in such order： physiological saline 〉 blood 〉 tap water 〉distilled water. The absorptive ability of the alginate fiber increases with the increase of M/G value and the concentration of sodium-alginate. Gradually increasing the concntration of coagulation bath, it increases until the concentration is up to 5%. However, it reduces with lasting coagulating tine.
文摘By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and 26 cases of hepatocellular carcinoma (HCC) patient. It was found that the upper limits of normal male and female were 1.1 and 1.4 ng/ml (expressed as M2-PyK) respectively. The plasma M-PyK in HCC patients was significant increased to above 5 times of average normal level, the positive rate was about 95%. In 6 cases of subclinical small hepatocarcinoma and 7 cases of HCC patient with normal serum alpha-fetal protein level, the mean plasma M-PyK value was also increased. Whereas the plasma M-PyK level in acute or chronic hepatitis and other benign diseases were normal. After the HCC being resected, the plasma M-PyK returned to normal, but increased again in the cases of recurrent hepatocarcinoma, suggesting that the increased M-PyK in the plasma of HCC patient was criginated from M2-type PyK in HCC tissue. Therefore, plasma M-PyK may become a new micro-level index of hepatocarcinoma.
