Immunofluorescent Study of Human MⅡ Oocytes Failed to Fertilize after IVF and ICSI

Objective To discuss the reason why human M Ⅱ oocytes failed to fertilize after IVF and ICSI. Methods The unfertilized human MⅡ oocytes were collected 24-48 h after IVF and ICSI and stained for immunoflurescence and PI counterstain. The types of fertilization failure were identified under the fluorescence microscopy. Results About 55.8% oocytes in IVF were found no sperm in them, which were more than that in ICSI （9.7%） （P〈0.01）. About 14.9% oocytes in IVF and 58.1% in ICSI displayed oocyte activation failure. The difference was significant （P〈0.01）. Defects in pronuclear formation and or migration was found in a similar proportion of oocytes both after IVF （25.3%） and ICSI （32.3%）（P〉0.05）. There were 3.9% oocytes with other abnormalities were observed in IVF but none in ICSI. Conclusion The main reason of fertilization failure after IVF was no sperm penetration. However fertilization failure after ICSI was mainly associated with incomplete oocyte activation.

长江12.5m深水航道二期工程对江阴港发展的影响

在调研江阴港腹地经济、港口发展、沿江企业需求的基础上,采取定性分析与定量计算相结合的研究方法,研究长江南京以下12.5m深水航道二期工程对江阴港发展的影响。分析得出:1)12.5m深水航道二期工程的实施,有力提升了长江南京—南通段航道通航条件,为开普敦型船舶进江阴港创造了有利条件。2)船舶大型化大幅提升了实载率,不仅让江阴港吞吐量实现大幅增长,而且给江阴港口企业减少了大量的物流成本,促进了船舶运输组织方式的优化调整,还减少了船舶污染和碳排放,推动港口的绿色发展。