Avian influenza virus (AIV) is a highly variable pathogen. The M2 gene of AIV is a transmembrane protein. It has highly conservative antigenic epitopes and is a potential antigen for cross-protection. In this study, t...Avian influenza virus (AIV) is a highly variable pathogen. The M2 gene of AIV is a transmembrane protein. It has highly conservative antigenic epitopes and is a potential antigen for cross-protection. In this study, the recombinants containing the full-length M2 and the transmembrane segment deleted M2 were constructed respectively. When measuring the expression of the two constructs, we found that the full-length M2 failed to express in the prokaryotic expression system, whereas transmembrane segment deleted M2 was highly expressed as fusion protein in a soluble form. The fusion protein reacted with SPF chicken serum specific for AIV A/goose / Guangdong /1996(H5N1). The protein was purified by GST purification system and the purified protein was used to prepare anti-M2 serum in mice. Immunofluorescence test demonstrated the binding of the antiserum with AIV (H5N1) infected MDCK cells. The results suggest that the recombinant transmembrane segment deleted M2 could provide good antigenicity.展开更多
Gastrointestinal(GI) cancer is one of the most common causes of cancer-related deaths worldwide.Tumor markers are valuable in detecting post-surgical recurrence or in monitoring response to chemotherapy.Pyruvate kinas...Gastrointestinal(GI) cancer is one of the most common causes of cancer-related deaths worldwide.Tumor markers are valuable in detecting post-surgical recurrence or in monitoring response to chemotherapy.Pyruvate kinase isoform M2(PKM2),a glycolytic enzyme catalyzing conversion of phosphoenolpyruvate(PEP) to pyruvate,confers a growth advantage to the tumor cells and enables them to adapt to the tumor microenvironment.In this review,we have summarized current research on the expression and regulation of PKM2 in tumor cells,and its potential role in GI carcinogenesis and progression.Furthermore,we have also discussed the potential of PKM2 as a diagnostic and screening marker,and a therapeutic target in GI cancer.展开更多
文摘Avian influenza virus (AIV) is a highly variable pathogen. The M2 gene of AIV is a transmembrane protein. It has highly conservative antigenic epitopes and is a potential antigen for cross-protection. In this study, the recombinants containing the full-length M2 and the transmembrane segment deleted M2 were constructed respectively. When measuring the expression of the two constructs, we found that the full-length M2 failed to express in the prokaryotic expression system, whereas transmembrane segment deleted M2 was highly expressed as fusion protein in a soluble form. The fusion protein reacted with SPF chicken serum specific for AIV A/goose / Guangdong /1996(H5N1). The protein was purified by GST purification system and the purified protein was used to prepare anti-M2 serum in mice. Immunofluorescence test demonstrated the binding of the antiserum with AIV (H5N1) infected MDCK cells. The results suggest that the recombinant transmembrane segment deleted M2 could provide good antigenicity.
基金supported by the grants from ‘San Ming’ Project of Shenzhen city,China(No.SZSM201612051)Municipal Health Planning Commission Fund of Shenzhen city,China(No.201601004,No.SZXJ2017078 and No.SXZJ2018084)
文摘Gastrointestinal(GI) cancer is one of the most common causes of cancer-related deaths worldwide.Tumor markers are valuable in detecting post-surgical recurrence or in monitoring response to chemotherapy.Pyruvate kinase isoform M2(PKM2),a glycolytic enzyme catalyzing conversion of phosphoenolpyruvate(PEP) to pyruvate,confers a growth advantage to the tumor cells and enables them to adapt to the tumor microenvironment.In this review,we have summarized current research on the expression and regulation of PKM2 in tumor cells,and its potential role in GI carcinogenesis and progression.Furthermore,we have also discussed the potential of PKM2 as a diagnostic and screening marker,and a therapeutic target in GI cancer.
