Wnts are powerful regulators of cell proliferation and differentiation. Activation of Wnt signalling in many tissues has also been associated with cancer. RNAi is a process of posttranscriptional gene silencing, by wh...Wnts are powerful regulators of cell proliferation and differentiation. Activation of Wnt signalling in many tissues has also been associated with cancer. RNAi is a process of posttranscriptional gene silencing, by which dsRNA induces sequence-specific degradation of homologous gene transcripts. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this study the pAVU6＋27 vector, which has Sail and Xbal clone sites, was used to construct the siRNA expression vectors for mouse wnt9a. Four kinds of small interfering RNA inserts were designed, synthesized and visually tested for efficacy by in situ hybridization, the results demonstrated that dramatically reduced wnt9a signals were observed in the cells transfected with U6＋27 cassettes with anti-wnt9a hairpin siRNA inserts compared with the untransfected. The results of flow cytometry analysis showed that the cell proliferation was promoted after lowering expression of the mouse wnt9a in MA891 cells by RNAi. All that suggest the expression level of mouse wnt9a in breast cancer MA891 cells may play a role in adjusting the rate of proliferation.展开更多
Animal experimental systems are particularly useful for the study of human breast cancer. An ideal model shoulcl be easy to use, closely mimicking human physiopathology and has a stable tumor morbidity. The cell line ...Animal experimental systems are particularly useful for the study of human breast cancer. An ideal model shoulcl be easy to use, closely mimicking human physiopathology and has a stable tumor morbidity. The cell line MA891 was established from a spontaneous TA2 mouse mammary carcinoma by Cancer Institute of Chinese Academy of Medical Sciences. 3 Some researches indicated that MA891 had a very low immunogenecity and maintained a high metastatic potential in vivo. So it has been used as a better grafted mouse tumor model for studying cancer physiopathology and metastasis in human for years. However, about the biological characteristic and the histopathologic feature of this model there has been a lack of investigations.展开更多
文摘Wnts are powerful regulators of cell proliferation and differentiation. Activation of Wnt signalling in many tissues has also been associated with cancer. RNAi is a process of posttranscriptional gene silencing, by which dsRNA induces sequence-specific degradation of homologous gene transcripts. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this study the pAVU6＋27 vector, which has Sail and Xbal clone sites, was used to construct the siRNA expression vectors for mouse wnt9a. Four kinds of small interfering RNA inserts were designed, synthesized and visually tested for efficacy by in situ hybridization, the results demonstrated that dramatically reduced wnt9a signals were observed in the cells transfected with U6＋27 cassettes with anti-wnt9a hairpin siRNA inserts compared with the untransfected. The results of flow cytometry analysis showed that the cell proliferation was promoted after lowering expression of the mouse wnt9a in MA891 cells by RNAi. All that suggest the expression level of mouse wnt9a in breast cancer MA891 cells may play a role in adjusting the rate of proliferation.
文摘Animal experimental systems are particularly useful for the study of human breast cancer. An ideal model shoulcl be easy to use, closely mimicking human physiopathology and has a stable tumor morbidity. The cell line MA891 was established from a spontaneous TA2 mouse mammary carcinoma by Cancer Institute of Chinese Academy of Medical Sciences. 3 Some researches indicated that MA891 had a very low immunogenecity and maintained a high metastatic potential in vivo. So it has been used as a better grafted mouse tumor model for studying cancer physiopathology and metastasis in human for years. However, about the biological characteristic and the histopathologic feature of this model there has been a lack of investigations.
