基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生...基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。展开更多
New matrix, metal-phthalocyanine （MPc）, of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） was used for analysis of small molecules （usually 〈500 Da）. By using MPcs a...New matrix, metal-phthalocyanine （MPc）, of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） was used for analysis of small molecules （usually 〈500 Da）. By using MPcs as matrices, small molecular samples were moved to high mass-to-charge region where there was no interference caused by the traditional matrices. The mass of the target analvte was obtained by simple calculation.展开更多
目的利用UPLC-Q-TOF-MS/MS技术和网络药理学探讨尖尾芋抗乳腺癌物质基础及作用机制。方法结合MassBank等数据库及现有文献研究,鉴定尖尾芋醇提物的化学成分,通过TCMSP、GeneCards等数据库筛选尖尾芋抗乳腺癌的作用靶点,使用STRING数据库...目的利用UPLC-Q-TOF-MS/MS技术和网络药理学探讨尖尾芋抗乳腺癌物质基础及作用机制。方法结合MassBank等数据库及现有文献研究,鉴定尖尾芋醇提物的化学成分,通过TCMSP、GeneCards等数据库筛选尖尾芋抗乳腺癌的作用靶点,使用STRING数据库和Cytoscape 3.9.0构建关键靶点蛋白相互作用(protein-protein interaction,PPI)网络;通过DAVID数据库对关键靶点进行基因本体论(gene ontology,GO)功能与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析,最后借助Cytoscape 3.9.0构建“成分-基因-通路”互作网络图。结果从尖尾芋醇提物中共鉴定18个成分,包括生物碱类(1,3,10,12)、苯丙素类(2,8,18)、黄酮类(6,7,9,11,15)等;基于鉴定出的化合物通过网络药理学得到429个潜在作用靶点;PPI分析发现PIK3CA、PIK3R1、MAPK1等10个核心靶点,富集分析发现核心靶点可能通过调控癌症通路发挥抗乳腺癌作用,“成分-基因-通路”互作网络图显示生物碱类成分小檗碱及黄酮类成分山柰酚、木犀草素可能是尖尾芋醇提物发挥药效的主要活性成分,其机制与凋亡相关。结论本研究初步探究了尖尾芋醇提物抗乳腺癌活性成分为生物碱及黄酮类成分,其作用机制与细胞凋亡相关,为进一步开展尖尾芋醇提物抗乳腺癌的药效物质基础及作用机制研究提供了新的思路和线索。展开更多
Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based pu...Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps.展开更多
文摘基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。
基金supported financially by NSFC(Nos.90717120 and 20435030),MOST(No.2007CB714504)Sino German Center for Research Promotion(No.GZ364) and CAS(No.KJCX2-YW-H11).
文摘New matrix, metal-phthalocyanine （MPc）, of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） was used for analysis of small molecules （usually 〈500 Da）. By using MPcs as matrices, small molecular samples were moved to high mass-to-charge region where there was no interference caused by the traditional matrices. The mass of the target analvte was obtained by simple calculation.
文摘目的利用UPLC-Q-TOF-MS/MS技术和网络药理学探讨尖尾芋抗乳腺癌物质基础及作用机制。方法结合MassBank等数据库及现有文献研究,鉴定尖尾芋醇提物的化学成分,通过TCMSP、GeneCards等数据库筛选尖尾芋抗乳腺癌的作用靶点,使用STRING数据库和Cytoscape 3.9.0构建关键靶点蛋白相互作用(protein-protein interaction,PPI)网络;通过DAVID数据库对关键靶点进行基因本体论(gene ontology,GO)功能与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析,最后借助Cytoscape 3.9.0构建“成分-基因-通路”互作网络图。结果从尖尾芋醇提物中共鉴定18个成分,包括生物碱类(1,3,10,12)、苯丙素类(2,8,18)、黄酮类(6,7,9,11,15)等;基于鉴定出的化合物通过网络药理学得到429个潜在作用靶点;PPI分析发现PIK3CA、PIK3R1、MAPK1等10个核心靶点,富集分析发现核心靶点可能通过调控癌症通路发挥抗乳腺癌作用,“成分-基因-通路”互作网络图显示生物碱类成分小檗碱及黄酮类成分山柰酚、木犀草素可能是尖尾芋醇提物发挥药效的主要活性成分,其机制与凋亡相关。结论本研究初步探究了尖尾芋醇提物抗乳腺癌活性成分为生物碱及黄酮类成分,其作用机制与细胞凋亡相关,为进一步开展尖尾芋醇提物抗乳腺癌的药效物质基础及作用机制研究提供了新的思路和线索。
基金Supported by the National Natural Science Foundation of China(No.30600458)
文摘Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps.
