Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were construc...Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).展开更多
椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同...椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同种群对生长和胴体性状的影响不一,也未见将两位点进行组合研究。因此,本试验采用PCR扩增和PCR-RFLP方法对399头150日龄的美系大白猪群体VRTN基因g.20311_20312ins291位点和MC4R基因g.Asp298Asn位点的多态性进行检测,并分析其与生长和胴体性状的关联性。多态性分析结果表明,VRTN基因g.20311_20312ins291位点在大白猪群体中有Ins/Ins、Ins/Del和Del/Del 3种基因型,基因型频率分别为0.165、0.509和0.326;MC4R基因Asp298Asn位点在大白猪群体中存在GG、AG和AA 3种基因型,基因型频率分别为0.040、0.213、0.747。性状关联分析结果显示,VRTN基因g.20311_20312ins291位点对眼肌面积、瘦肉率有显著影响(P<0.05),对其他性状无显著影响(P>0.05);MC4R基因Asp298Asn位点对体重、日增重、背膘厚、眼肌面积及瘦肉率等性状均有显著影响(P<0.05)。合并基因型对体重、日增重、背膘厚及瘦肉率等4个性状有显著影响(P<0.05),对眼肌面积无显著影响(P>0.05);携带MC4R A等位基因的个体,除Del/Del-AG组合基因型外,无论VRTN是何种基因型,体重和日增重均能表现显著优势(P<0.05),其中Ins/Ins-AA基因型组合最佳。综上可知,VRTN和MC4R基因对大白猪生长和胴体性状具有一定影响,可为后续应用VRTN和MC4R基因辅助选育大白猪提供参考。展开更多
基金supported by a loth 5-year personwith-ability prject of Anhui province,Natural Science Foundation of Anhui Educational Institution(No.2006KJ/69B)Key Scientific Project of Anhui Provincial Department of Science and Technology(No.07020303055,08010302070)
文摘Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).
