AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells ...AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells and then to analyse the Bcl 2 protein expression in the cells by Western blotting. To observe the morphology alteration, detect the G1 phase arrest by FCM, inhibition of proliferation by MTT method and tumorigenicity in nude mice. RESULTS The MGC anti bcl 2 cells shows contact inhibition, morphological alteration, from round or near round to shuttle like, decelerated growth rate, G1 phase arrest and weakened tumorigenicity in nude mice unlike the control (MGC neo cells). CONCLUSION Antisense RNA to bcl 2, not only can induce apoptosis, but also reverse the biological behavior of MGC 803 cells. This would be a potential application to the gene therapy for stomach cancers.展开更多
文摘AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells and then to analyse the Bcl 2 protein expression in the cells by Western blotting. To observe the morphology alteration, detect the G1 phase arrest by FCM, inhibition of proliferation by MTT method and tumorigenicity in nude mice. RESULTS The MGC anti bcl 2 cells shows contact inhibition, morphological alteration, from round or near round to shuttle like, decelerated growth rate, G1 phase arrest and weakened tumorigenicity in nude mice unlike the control (MGC neo cells). CONCLUSION Antisense RNA to bcl 2, not only can induce apoptosis, but also reverse the biological behavior of MGC 803 cells. This would be a potential application to the gene therapy for stomach cancers.
