Hydrogen sulfide （H2S） is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of tw...Hydrogen sulfide （H2S） is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of two cysteine desulfhydrases, and exogenous H2S donor （sodium hydrosulfide, NariS） and H2S scavenger （hypotaurine, HT） pre-treated plants were used to dissect the involvement of H2S in plant stress responses. The cysteine desulfhydrases overexpressing plants and NariS pre-treated plants exhibited higher endogenous H2S level and improved abiotic stress tolerance and biotic stress resistance, while cysteine desulfhydrases knockdown plants and HT pre-treated plants displayed lower endogenous H2S level and decreased stress resistance. Moreover, H2S upregulated the transcripts of multiple abiotic and biotic stress-related genes, and inhibited reactive oxygen species （ROS） accumulation. Interest- ingly, MlR393-mediated auxin signaling including MIR393a/b and their target genes （TIR1, AFB1, AFB2, and AFB3） was transcrip-tionally regulated by H2S, and was related with H2S-induced antibacterial resistance. Moreover, H2S regulated 50 carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines. Taken together, these results indicated that cysteine desulfhydrase and H2S conferred abiotic stress tolerance and biotic stress resistance, via affecting the stress-related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393-targeted auxin receptors.展开更多
基金supported by the National Natural Science Foundation of China(31370302)"the Hundred Talents Program"(54Y154761001076 and 29Y32963100263) to Zhulong Chan+2 种基金by the National Natural Science Foundation of China(31200194)Youth Innovation Promotion Association of Chinese Academy of Sciences(29Y42937100437)the Knowledge Innovative Key Program of Chinese Academy of Sciences(55Y45544600544) to Haitao Shi
文摘Hydrogen sulfide （H2S） is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of two cysteine desulfhydrases, and exogenous H2S donor （sodium hydrosulfide, NariS） and H2S scavenger （hypotaurine, HT） pre-treated plants were used to dissect the involvement of H2S in plant stress responses. The cysteine desulfhydrases overexpressing plants and NariS pre-treated plants exhibited higher endogenous H2S level and improved abiotic stress tolerance and biotic stress resistance, while cysteine desulfhydrases knockdown plants and HT pre-treated plants displayed lower endogenous H2S level and decreased stress resistance. Moreover, H2S upregulated the transcripts of multiple abiotic and biotic stress-related genes, and inhibited reactive oxygen species （ROS） accumulation. Interest- ingly, MlR393-mediated auxin signaling including MIR393a/b and their target genes （TIR1, AFB1, AFB2, and AFB3） was transcrip-tionally regulated by H2S, and was related with H2S-induced antibacterial resistance. Moreover, H2S regulated 50 carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines. Taken together, these results indicated that cysteine desulfhydrase and H2S conferred abiotic stress tolerance and biotic stress resistance, via affecting the stress-related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393-targeted auxin receptors.
