Human immune suppression is inducible by trichosanthin via CD8 cell-mediated pathway

Thichosanthin(Tk), a polypeptide with 249 amino acid residues isolated and purified from a Chinese medicinal herb, showed the capability of inducing abortion and was able to inhibit tumor growth and HIV replication. Owing to sequence homology of the peptide with a ribosomeinactivating protein, the downward activity of Tk was suggested to be related to its cytotoxic property. We report here, however, that Tk could exert potent inhibitory effects on human lymphoproliferative responses in vitro to allogeneic, mitogenic and soluble antigens with 50% inhibition doses ranged between 0.05 and 0.5 μg/ml. The lowresponsiveness caused by Tk was not due to toxic cytolysis. Rather, evidences suggested that, in the dose range adopted, the Tk-induced inhibition was attributable, at least in part, to immune suppression, in view of (1) Tk was more effective in the early stage of alloreactivity; (2)Suppression also occurred if responder cells were pulsetreated with Tk rather than cocultured; (3) Irradiated Tk-pulsed cells were capable of inducing suppression in a Tk-free culture; (4) Suppression could also be transferred by the supernatants of Tk-pulsed cultured cells; (5) Tkinduced immune suppression was diminished by depletion of CD8+ cells from the culture, and, finally; (6) Adding CD8+ cells back to the culture could restore the suppres Trichosanthin-induced humall immune suppression sion. Thus the possibility that Tk might function as a down-regulator by immunological mechanisms in human immune responses is discussed.

Up-regulation of Tim-3 Expression Contributes to Development of Burn-induced T Cell Immune Suppression in Mice

T cell immunoglobulin and mucin domain 3 （Tim-3） is well known to negatively regulate T cells responses, but its role in burn-induced T cells immune suppression remains unclear. In the present study, in order to identify the relationship between Tim-3 expression and post-burn T cells immune suppression, C57BL/6 mice were subjected to burn injury or sham injury, and the liver and spleen were harvested at the day 1 after operation. The expression level of Tim-3 on hepatic or splenic T cells and the functional properties of Tim-3＋ T cells were evaluated. It was found burn injury induced dramatically elevated Tim-3 expression on both hepatic and splenic CD4＋ and CD8＋ T cells in contrast with the post-burn depletion of T cells. Furthermore, Tim-3 expression was correlated with the suppressive phenotype of T cells following burn injury, including increased expression of anti-inflammatory cytokine IL-10, decreased expression of pro-inflammatory cytokines IFN-γ and TNF-α, reduced T cell proliferation and elevated co-expression of Tim-3 and PD-1. Moreover, Tim-3＋ T cells subsets were more prone to spontaneous apoptosis than Tim-3- T cells subsets. Our findings reinforce the idea that the up-regulated expression of Tim-3 on T cells after burn injury plays an important role in the development and maintenance of burn-induced T cell immune suppression.

The Effect of TSH Suppression Therapy on the Efficacy and Immune Function of Postoperative Patients with Thyroid Cancer

Objective:To investigate the efficacy and immune function of thyroid stimulating hormone(TSH)suppression therapy in postoperative thyroid cancer patients.Methods:Sixty thyroid cancer patients admitted from July 2020–July 2022 were recruited and randomly divided into two groups.The control group(30 patients)received hormone replacement therapy,while the study group(30 patients)received TSH suppression therapy.The thyroid function,clinical efficacy,immune function,and tumor markers of the two groups were compared.Results:After treatment,the levels of free triiodothyronine(FT3)and thyroxine(FT4)in both groups increased significantly,while TSH levels decreased significantly.Moreover,the magnitude of change in the study group was greater than that in the control group(P<0.05).The total effective rate in the study group was significantly higher as compared to the control group(P<0.05).After treatment,the levels of CD3+and CD4+cells in both groups of patients increased significantly,with the study group showing significantly higher levels than the control group,whereas the level of CD8+cells decreased significantly,with the study group having lower levels than the control group(P<0.05).After treatment,the levels of Tg and CEA in both groups were significantly lowered as compared to before treatment,and the levels of Tg and CEA in the study group were significantly lower than the control group(P<0.05).Conclusion:TSH suppression therapy in postoperative thyroid cancer patients can improve thyroid function,suppress the levels of tumor markers,and enhance immune function,thereby achieving good clinical outcomes.

Aiming to immune elimination of ovarian cancer stem cells

Ovarian cancer accounts for only 3% of all cancers in women, but it causes more deaths than any other gynecologic cancer. Treatment with chemotherapy and cytoreductive surgery shows a good response to the therapy. However, in a large proportion of the patients the tumor grows back within a few years. Cancer stem cells, that are less responsive to these treatments, are blamed for this recurrence of disease. Immune therapy either cellular or humoral is a novel concept to treat cancer. It is based on the notice that immune cells invade the tumor. However, the tumor invest heavily to escape from immune elimination by recruiting several immune suppressive mechanisms. These processes are normally in place to limit excessive immune activation and prevent autoimmune phenomena. Here, we discuss current knowledge about the immune(suppressive)status in ovarian cancer. Moreover, we discuss the immunological targets of ovarian cancer stem cells.

Effect of Fuzheng Jiedu granule on immunological function and level of immune-related cytokines in immune-suppressed mice

Fuzheng Jiedu granule exhibits a number of health benefits and it is thought that the mechanisms involved in these effects are due to the modulation of immunity. In this article, we studied the effect of Fuzheng Jiedu granule on immunological function and the expression of immune-related cytokines in immune-suppressed mice. 72 mice were randomly divided into six groups, with 12 in each group. The control groups included an untreated group, a negative control group（Cyclophosphamide） and a positive control group（Astragalus polysaccharide）. There were three treated groups, which were given different doses of Fuzheng Jiedu granule： a low dose（100 mg kg^（–1））, a medium dose（400 mg kg^（–1）） and a high dose（600 mg kg^（–1））. With the exception of the untreated control animals, each group received an intraperitoneal injection of Cyclophosphamide（100 mg kg^（–1）） for 3 days to establish the immune-suppressed model. Mice were then treated for 19 consecutive days and, 24 h after the last treatment, blood was taken for the eyeballs and serum separation was performed. Analysis was made of the levels of related cytokines（IgA, IgG, IgM, IL-6, IFN-γ, C3, C4 and TNF-α）, the transformation of lymphocytes and the immune organ indexes. The results showed that Fuzheng Jiedu granule can improve the levels of cytokines, the rate of proliferation of lymphocytes and the immune organ indexes of immune-suppressed mice.

Immune phenotypes of prostate cancer cells: Evidence of epithelial immune cell-like transition?

Prostate cancers(PCa)have been reported to actively suppress antitumor immune responses by creating an immune-suppressive microenvironment.There is mounting evidence that PCas may undergo an‘‘Epithelial Immune Cell-like Transition’’(EIT)by expressing molecules conventionally associated with immune cells(e.g.,a variety of cytokines/receptors,immune transcription factors,Ig motifs,and immune checkpoint molecules),which subsequently results in the suppression of anti-cancer immune activity within the tumor microenvironment.Recent progress within the field of immune therapy has underscored the importance of immune checkpoint molecules in cancer development,thus leading to the development of novel immunotherapeutic approaches.Here,we review the expression of select immune checkpoint molecules in PCa epithelial and associated immune cells,with particular emphasis on clinical data supporting the concept of an EIT-mediated phenotype in PCa.Furthermore,we summarize current advances in anti-immune checkpoint therapies,and provide perspectives on their potential applicability.

Does steroid-free immunosuppression improve the outcome in kidney transplant recipients compared to conventional protocols?

Steroids continue to be the cornerstone of immune suppression since the early days of organ transplantation.Steroids are key component of induction protocols,maintenance therapy and in the treatment of various forms of rejection.Prolonged steroid use resulted in significant side effects on almost all the body organs owing to the presence of steroid receptors in most of the mammalian cells.Kidney allograft recipients had to accept the short and long term complications of steroids because of lack of effective alternatives.This situation changed with the introduction of newer and more effective immune suppression agents with a relatively more acceptable side effect profile.As a result,the clinicians have been contemplating if it is the time to abandon the unquestionable reliance on maintenance steroids in modern transplantation practice.This review aims to evaluate the safety and efficacy of various steroid-minimization approaches(steroid avoidance,early steroid withdrawal,and late steroid withdrawal)in kidney transplant recipients.A meticulous electronic search was conducted through the available data resources like SCOPUS,MEDLINE,and Liverpool University library e-resources.Relevant articles obtained through our search were included.A total number of 90 articles were eligible to be included in this review[34 randomised controlled trials(RCT)and 56 articles of other research modalities].All articles were evaluating the safety and efficacy of various steroidfree approaches in comparison to maintenance steroids.We will cover only the RCT articles in this review.If used in right clinical context,steroid-free protocols proved to be comparable to steroid-based maintenance therapy.The appropriate approach should be tailored individually according to each recipient immunological challenges and clinical condition.

Effects of Bortezomib and Dexamethasone combination on treating senile multiple myeloma and influence on immune suppressed factors and immune cell levels

Objective:To investigate the effects of Bortezomib and Dexamethasone combination on treating senile multiple myeloma and influence on immune suppressed factors and immune cell levels.Methods:A total of 80 cases of patients with multiple myeloma treated in our hospital from Oct 2013 to Jul 2015 were selected as investigate subjects. They were randomly divided to be observation group consisted of 43 cases and control group consisted of 37 cases. For observation group, treatment of Bortezomib and Dexamethasone combination was provided. For control group, Vincristine + Epirubicin + Dexamethasone treatment was provided. After three courses, effects on two groups of patients were compared, and immune suppressed factors and immune cell levels before and after treatment in different periods were compared.Results:After three courses of treatment, the total effective rate in observation group was significantly higher than control group. Before treatment, IL-6, IL-17, TGF-β, CD3+CD4+, CD3+CD8+ and CD3+CD4+/CD3+CD8+ between the two group of patients were compared, no significant difference showed;After treatment for 6 weeks, IL-6, IL-17 levels in observation group were significantly decreased comparing with the same group before treatment;After treatment for 12 weeks, IL-6, IL-17 and TGF-β levels in observation group were significantly decreased comparing with the same group before treatment;After treatment, IL-6, IL-17 levels in observation group were significantly lower than control group at the same phase. After treatment for 12 weeks, CD3+CD4+, CD3+CD4+/CD3+CD8+ in observation group were significantly higher than the same group before treatment, and significantly higher than control group at the same phase;CD3+CD8+ in observation group was significantly lower than the same group before treatment, and significantly lower than control group at the same phase. Conclusion:Compared with Vincristine + Epirubicin + Dexamethasone treatment, Bortezomib and Dexamethasone combination on treating senile multiple myeloma had more significant effects, which deserves further clinical researches.

Innate-like CD4 T cells selected by thymocytes suppress adaptive immune responses against bacterial infections

We have reported a new innate-like CD4 T cell population that expresses cell surface makers of effector/memory cells and produce Th1 and Th2 cytokines immediately upon activation. Unlike conventional CD4 T cells that are selected by thymic epithelial cells, these CD4 T cells, named T-CD4 T cells, are selected by MHC class II expressing thymocytes. Previously, we showed that the presence of T-CD4 T cells protected mice from airway inflammation suggesting an immune regulatory role of T-CD4 T cells. To further understand the function of T-CD4 T cells, we investigated immune responses mediated by T-CD4 T cells during bacterial infection because the generation of antigen specific CD4 T cells contributes to clearance of infection and for the development of immune memory. The current study shows a suppressive effect of T-CD4 T cells on both CD8 and CD4 T cell-mediated immune responses during Listeria and Helicobacter infections. In the mouse model of Listeria monocytogenes infection, T-CD4 T cells resulted in decreasedfrequency of Listeria-specific CD8 T cells and the killing activity of them. Furthermore, mice with T-CD4 T cells developed poor immune memory, demonstrated by reduced expansion of antigen-specific T cells and high bacterial burden upon re-infection. Similarly, the presence of T-CD4 T cells suppressed the generation of antigen-specific CD4 T cells in Helicobacter pylori infected mice. Thus, our studies reveal a novel function of T-CD4 T cells in sup-pressing anti-bacterial immunity.

<i>Leishmania donovani</i>-Induced Immune Dysregulation among Sudanese Patients with Visceral and Post Kala-Azar Dermal Leishmaniases: Possible Roles in Pathogenesis

L. donovani infections (visceral and post kala-azar dermal leishmaniases) are characterized by infection-induced reversible immune suppression. Autoimmunity is a well-documented phenomenon among patients with primary immune deficiencies. This study aimed to study auto-immune phenomena accompanying L. donovani infections. In a prospective case-controlled study and following informed consent, 155 individuals with visceral leishmaniasis (VL;n = 62), post kala-azar dermal leishmaniasis (PKDL;n = 31) and apparently healthy volunteers (n = 62) were recruited. Sera antinuclear (ANA), anti-dsDNA, anti-thyroid peroxidase (TPO), anti-smooth muscles (ASMA) and F-actin auto-antibodies were measured using ELISA and indirect immune-fluorescence assay. The mean ages of VL, PDKL patients and apparently healthy volunteers were: 17.5 ± 12.5, 15.0 ± 7.0 and 17.5 ± 9.5 years with Male:Female ratios of 2:0, 1:2 and 1:5 respectively. Significantly high frequencies of F-actin (74.2%;46/62) and ASMA (50%;31/62) auto-antibodies were seen among VL patients (p = 0.003, p = 0.001) compared to apparently healthy volunteers. Likewise, significantly high frequencies of F-actin (64.5%;20/31;p = 0.001), ASMA (42%;13/31;p = 0.003), ANA (36%;11/31;p = 0.001) and anti-dsDNA (16%;5/31;p = 0.01) auto-antibodies were seen among PKDL patients. Development of tissue-based autoantibodies in L. donovani infections probably indicates loss of peripheral tolerance with activation of circulating auto-reactive T and B cells probably contributing to disease pathogenesis (increased bilirubin/liver enzymes, prolonged QT interval/arrythmias and blood cytopenias). In conclusion, L. donovani infection-induced immune suppression with development of tissue-based auto-antibodies is prevalent among Sudanese patients with VL and PKDL leishmaniases and contributes to some aspects of the disease pathogenesis.

益肾化瘀方对B细胞淋巴瘤患者化疗后骨髓抑制的疗效及免疫功能的影响

目的研究益肾化瘀方对B细胞淋巴瘤患者化疗后骨髓抑制的临床疗效,并观察其对免疫功能指标的影响。方法选取2019年1月—2021年12月本院收治的B细胞淋巴瘤患者共74例为研究对象,按随机数字表法将其随机分为中药辅助治疗组(n=37)和对照组(n=37)。其中,中药辅助治疗组在化疗结束后给予益肾化瘀方进行辅助治疗,对照组则仅给予常规对症支持治疗,比较两组的临床效果。结果中药辅助治疗组生活质量评分及卡氏评分(KPS)均高于对照组,且两组外周血三系细胞均升高(P<0.05),但中药辅助治疗组三系细胞计数较对照组显著升高(P<0.05)。化疗结束第14d,中医辅助治疗组T淋巴细胞亚群(CD3^(+),CD4^(+)、CD4^(+)/CD8^(+))水平均高于对照组(P<0.05)。结论益肾化瘀方有助于改善B细胞淋巴瘤患者化疗后的生活质量,促进其骨髓抑制的早期恢复,同时改善患者细胞免疫功能,值得临床推广。

HBeAg诱导的免疫激活和免疫抑制在慢性乙型肝炎中的作用

HBV感染诱导的慢性乙型肝炎是导致肝硬化和肝癌的重要危险因素。半个世纪前,HBeAg在HBV感染者血清中首次被发现,尽管HBeAg并不参与HBV在肝细胞中的感染或复制,但其已被证实可干扰宿主先天性和适应性免疫反应,在慢性HBV感染的过程中发挥着重要的免疫激活和免疫抑制作用。HBV对于感染的肝细胞并没有细胞毒性,免疫应答介导的抗病毒作用和炎症反应决定HBV是否被清除或者诱导肝脏炎症相关疾病。因此,本文对HBeAg的形成及其在慢性HBV感染中引起的免疫激活和免疫抑制机制进行综述,重点论述HBeAg对先天免疫和适应性免疫细胞所引起的不同免疫效应,阐述了其诱导免疫反应的两面性,并探讨HBeAg在慢性HBV感染不同阶段间的转换作用。

脓毒症免疫抑制机制及治疗策略

脓毒症是机体对感染反应失调引起的危及生命的器官功能障碍。随着研究的深入,对脓毒症的理解已经从单纯的炎症反应拓展到涉及免疫抑制和免疫失衡。本文综述了脓毒症免疫抑制的生物学机制和临床影响,评估了针对脓毒症免疫抑制的多种潜在治疗策略,包括免疫检查点抑制剂、免疫刺激性细胞因子、免疫球蛋白、胸腺素α1和间充质干细胞等。旨在为临床医生和研究者提供关于脓毒症免疫抑制的最新见解,并探索改善患者长期预后的新兴治疗方法。

顺铂联合人参皂苷Rh2对4T1乳腺癌荷瘤小鼠的抑瘤及免疫调控机制

目的探讨顺铂联合人参皂苷Rh2对4T1乳腺癌荷瘤小鼠的抑瘤及免疫调控机制。方法建立4T1乳腺癌荷瘤小鼠,分为模型组、Rh2组、顺铂组和联合用药组(顺铂+Rh2)。记录小鼠一般状态,观察肿瘤组织的病理结构,采用免疫组化法检测炎性因子IL-2、IL-10、IFN-γ和多药耐药蛋白P-糖蛋白(P-glycoprotein,Pgp)的表达水平,评价联合用药的抑瘤效果和免疫调节机制。结果模型组、Rh2组和联合用药组小鼠生活状态较好,顺铂组小鼠精神状态萎靡、运动能力下降、毛发枯槁脱落、食欲减退及腹泻。与模型组比较,各给药组小鼠的体重均降低、肿瘤体积均减小及肿瘤坏死面积均增大,肿瘤组织中IL-2、IFN-γ蛋白水平均明显增高,IL-10蛋白水平均明显降低,组间差异显著均有统计学意义(P<0.05);PgP蛋白水平在顺铂组明显增高,差异显著有统计学意义(P<0.05),其他各组间未见明显差异(P>0.05)。结论联合用药能够通过增强机体免疫功能及对抗肿瘤组织对顺铂的多药耐药性,从而加强治疗效果,该联合疗法具有重要的临床应用价值,为从中药中发现联合免疫治疗药物提供新的思路。

健脾养正方联合紫杉醇节律化疗对小鼠胃癌肺转移的实验观察

目的:观察健脾养正方联合紫杉醇节律化疗对小鼠胃癌肺转移的抑制作用,及对小鼠紫杉醇化疗所致的骨髓抑制和免疫损伤的影响。方法:30只615品系小鼠随机分为模型组、常规剂量的紫杉醇化疗组(PTX)、紫杉醇节律化疗组(MC-PTX),健脾养正方组(JPYZ),健脾养正方联合紫杉醇节律化疗组(MC-PTX+JPYZ),每组6只,小鼠尾静脉注射MFC细胞建立胃癌肺转移模型。给药2周后观察肿瘤浸润肺组织情况,计算肺转移抑制率;完整剥离脾、胸腺组织,计算胸腺指数、脾指数;HE染色观察骨髓病理组织学改变及骨髓细胞占骨髓腔比值;流式细胞仪检测骨髓细胞凋亡情况;血细胞分析检测外周血中白细胞、红细胞、血小板、血红蛋白的改变。结果:1.MC-PTX+JPYZ组肿瘤浸润及肺转移灶结节最少且抑瘤率最高(73.14%),显著高于其它组(P<0.05);2.与PTX组和MC-PTX组比较,MC-PTX+JPYZ组骨髓细胞占骨髓腔比值显著升高(P<0.05),骨髓细胞的早期凋亡率和总凋亡率降低明显,升高了紫杉醇化疗后骨髓抑制小鼠的白细胞水平(P<0.05)且对骨髓有明显的促恢复作用。3.与PTX组和MC-PTX组相比,MC-PTX+JPYZ组体质量、胸腺、脾指数均上升(P<0.05)。结论:健脾养正方联合紫杉醇节律化疗能显著增加紫杉醇治疗胃癌肺转移的疗效,显著减轻紫杉醇引起的骨髓抑制及免疫功能损伤。

基于同步采样SDFT的高性能单相锁相环方法研究

在电力机车及动车组的牵引传动控制系统中,网侧变流器通过锁相环获取相位信息并进行精确控制。牵引供电网电压的幅值及频率存在波动,而且网压经常出现严重畸变及含有大量谐波,复杂的电网环境对锁相环性能提出了很高的要求。文章结合同步采样,提出一种基于SDFT的高性能锁相环方法,并通过数字仿真及半实物仿真对所述方法的性能进行了验证。仿真结果表明,与其他几种主流的锁相环对比,该方法稳态锁相精度高,动态响应速度快,并有较强的谐波抑制能力与抗偏置能力,适宜在轨道交通领域应用。

Chronic social defeat stress‑induced depression reduces BCG efficacy by promoting regulatory T‑cell levels in mice

Despite the initial successes of the Bacillus Calmette-Guerin(BCG)vaccine in children,its efficacy against tuberculosis is highly variable.There is a lack of understanding about how mental conditions influence BCG vaccination.Here,we used the chronic social defeat stress(CSDS)model to explore the effects of depression on BCG vaccination efficacy.We observed higher lung and spleen bacterial loads and a lower organ index in depressed compared to BCG mice.Meanwhile,a relatively lower T cell protective efficacy was observed in both compared to control and BCG mice via a mycobacterium growth inhibition assay(MGIA).Cytokine expression of IL-12p40,IL-1β,IL-17,TNF-αand IFN-γwas reduced,whereas the expression of IL-10 and IL-5 was increased in the spleen of both compared to BCG mice.Moreover,the proportions of CD4^(+)IFN-γ^(+),CD8^(+)IFN-γ^(+)T lymphocytes and CD4^(+)effector/central memory T cells were reduced in the splenocytes of the depressed BCG mice.Depression promotes CD4^(+)regulatory T cells(Treg)and myeloid-derived suppressor cell(MDSC)generation in depressed mice,contributing to the reduced pro-inflammatory immune response upon BCG vaccination.This study provides insight into the decreased protective immunity by BCG vaccination attributable to depression in mice.

肺部感染性脓毒症患者支气管肺泡灌洗液中PD-1、PD-L1与免疫抑制及预后的相关性研究

目的探讨PD-1和PD-L1在支气管肺泡灌洗液(BALF)样本中作为检测标志物的可行性,为肺部感染性脓毒症患者的免疫抑制及预后评估提供有效指标。方法选取2022年7月至2023年12月江苏省昆山市第六人民医院及昆山市第一人民医院ICU病区的脓毒症患者为研究对象,采集患者静脉血和BALF,测定2种样本中程序性死亡蛋白-1(PD-1)、程序性死亡蛋白配体-1(PD-L1)的表达,以及静脉血中白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)的表达。结合SOFA、APACHEⅡ评分绘制受试者工作特征(ROC)曲线,分析PD-1、PD-L1与患者免疫抑制及预后的相关性。结果本研究共纳入60例符合标准的患者,根据28 d内的生存情况分为存活组(n=40)和死亡组(n=20)。存活组的IL-10表达高于死亡组(P<0.001),而PD-1、PD-L1及TNF-α表达低于死亡组(P<0.001);存活组的SOFA、APACHEⅡ评分较死亡组低(P<0.001);BALF中PD-1、PD-L1的检出率高于静脉血(P<0.001),(ROC)曲线优势明显。结论BALF中的PD-1和PD-L1可作为肺部感染性脓毒症患者用于评估预后的检测标志物。

石墨烯远红外对Lewis肺癌荷瘤小鼠顺铂化疗免疫损伤的修复作用

目的探究石墨烯远红外对顺铂化疗后的Lewis肺癌荷瘤小鼠相关免疫指标的影响。方法建立Lewis肺癌荷瘤小鼠免疫损伤模型,将小鼠分为模型对照组(CTL)、单独化疗组(DDP)、石墨烯低功率组(G-Low,30℃)、石墨烯高功率组(G-High,35℃)。检测小鼠的肿瘤体积增殖率、胸腺指数和脾脏指数、脾脏淋巴细胞增殖活性及NK细胞杀伤活性、外周血中白细胞数量及CD4^(+)T/CD8^(+)T的比值。结果与CTL组相比,DDP组小鼠胸腺指数、脾脏指数明显降低(P<0.01);与DDP组相比,G-Low组小鼠肿瘤抑制率显著提升(P<0.05),G-High组小鼠肿瘤抑制率极显著提高(P<0.01)。G-High组小鼠胸腺指数、白细胞数量、外周血CD4^(+)T/CD8^(+)T比值、脾脏NK细胞的杀伤活性均显著高于DDP组(P<0.05)。结论石墨烯远红外对顺铂化疗造成的Lewis肺癌荷瘤小鼠的免疫损伤具有良好的修复作用,且能显著抑制瘤体的生长。

补肾益气方对乳腺癌化疗后骨髓抑制及免疫功能影响的临床研究

目的探究补肾益气方对乳腺癌化疗后骨髓抑制及免疫功能的影响。方法选取解放军总医院第六医学中心2023年1月至2024年1月收治的110例经表柔比星+环磷酰胺序贯多西紫杉醇(EC-T)方案化疗的乳腺癌患者作为研究对象,采用随机方法分为对照组和观察组,各55例。其中对照组给予EC-T方案治疗,观察组在对照组基础上口服补肾益气方治疗。观察2组患者治疗前后血常规指标、细胞免疫功能、中医症状积分、生存质量评分(QLQ-C30)变化。结果治疗后,2组白细胞计数、中性粒细胞绝对值、血红蛋白、血小板计数水平均低于治疗前,但观察组均高于对照组(均P<0.05)。治疗后,2组CD_(3)^(+)、CD_(4)^(+)水平及CD_(4)^(+)/CD_(8)^(+)比值均低于治疗前,但观察组均高于对照组,CD_(8)^(+)水平均高于治疗前,但观察组低于对照组(均P<0.05)。治疗后,2组气短乏力、心悸失眠、头晕眼花、面色少华、畏寒肢冷、腰膝酸软积分均高于治疗前,但观察组均低于对照组(均P<0.05)。治疗7、20 d后,2组QLQ-C30评分均低于治疗前,但观察组均高于对照组[(75.2±2.1)分比(74.1±1.9)分、(78.9±2.2)分比(75.9±2.1)分](均P<0.05)。结论补肾益气方能够有效预防乳腺癌化疗后骨髓抑制情况,纠正化疗后免疫功能紊乱情况,同时亦能改善患者化疗后继发的临床症状,明显提高生存质量,可以作为乳腺癌化疗患者的辅助补充治疗。