Secretory carcinoma（SC）, previously described as mammary analogue secretory carcinoma（MASC）, is a recently described salivary gland tumor which morphologically resembles mammary secretory carcinoma. The first desc...Secretory carcinoma（SC）, previously described as mammary analogue secretory carcinoma（MASC）, is a recently described salivary gland tumor which morphologically resembles mammary secretory carcinoma. The first description of SC/MASC, reported by Skálová et al. in 2010, was as a rare salivary carcinoma imitating secretory carcinoma of the breast. SC/MASC is a unique salivary gland tumor with morphological overlap with acinic cell carcinoma（Aci CC）, mucoepidermoid carcinoma（MEC）, and adenocarcinoma not otherwise specified（ADCNOS）. SC/MASC shares similar clinicopathological features with Aci CC. As a critical difference between SC/MASC and Aci CC, SC/MASC characteristically has the chromosomal translocation t（12;15）（p13;q25） which leads to a fusion gene between the ETV6 gene on chromosome 12 and the NTRK3 gene on chromosome 15. This genetic background is an important differential diagnostic finding for excluding other salivary gland tumors and may be a critical factor determining the prognosis for patients with SC/MASC. Research in recent years has provided a large body of new data on SC/MASC and suggests the possibility that the ETV6-NTRK3 translocation could be a therapeutic target. Here, we review the morphological and clinicopathological features of SC/MASC and discuss new directions for therapy.展开更多
目的合成IL-6的编码基因,并在大肠杆菌中表达,获得纯化的融合蛋白IL-6。方法采用基于PAS(PCR-based accurate synthesis)的方法,设计全长拼接引物,合成基因IL-6,并将其插入载体pCzn1,获得的重组质粒pCzn1-IL-6,化学法将其转化入大肠杆菌...目的合成IL-6的编码基因,并在大肠杆菌中表达,获得纯化的融合蛋白IL-6。方法采用基于PAS(PCR-based accurate synthesis)的方法,设计全长拼接引物,合成基因IL-6,并将其插入载体pCzn1,获得的重组质粒pCzn1-IL-6,化学法将其转化入大肠杆菌TOP10进行克隆。将克隆得到的阳性克隆子转化入大肠杆菌Arctic Express后用IPTG诱导剂诱导融合蛋白的表达,SDS-PAGE和Western Blot鉴定蛋白表达效果。结果经测序和双酶切验证,正确构建了重组质粒pCzn1-IL-6,重组质粒成功转入表达菌株,IL-6原核蛋白在表达菌株中以包涵体和可溶性形式稳定表达,通过Ni柱亲和纯化获得纯化的目标蛋白。结论 IL-6原核蛋白在大肠杆菌中成功表达,为进一步的应用研究打下基础。展开更多
文摘Secretory carcinoma（SC）, previously described as mammary analogue secretory carcinoma（MASC）, is a recently described salivary gland tumor which morphologically resembles mammary secretory carcinoma. The first description of SC/MASC, reported by Skálová et al. in 2010, was as a rare salivary carcinoma imitating secretory carcinoma of the breast. SC/MASC is a unique salivary gland tumor with morphological overlap with acinic cell carcinoma（Aci CC）, mucoepidermoid carcinoma（MEC）, and adenocarcinoma not otherwise specified（ADCNOS）. SC/MASC shares similar clinicopathological features with Aci CC. As a critical difference between SC/MASC and Aci CC, SC/MASC characteristically has the chromosomal translocation t（12;15）（p13;q25） which leads to a fusion gene between the ETV6 gene on chromosome 12 and the NTRK3 gene on chromosome 15. This genetic background is an important differential diagnostic finding for excluding other salivary gland tumors and may be a critical factor determining the prognosis for patients with SC/MASC. Research in recent years has provided a large body of new data on SC/MASC and suggests the possibility that the ETV6-NTRK3 translocation could be a therapeutic target. Here, we review the morphological and clinicopathological features of SC/MASC and discuss new directions for therapy.
