目的研究河北省鼠疫菌株多位点可变数串联重复序列(multiple-locus variable-number tandem-repeat analysis,MLVA)分型特征,分析其流行病学意义。方法采用MLVA(14+12)分型策略设计引物。利用聚合酶链式反应(PCR)、毛细管电泳方法和Bio ...目的研究河北省鼠疫菌株多位点可变数串联重复序列(multiple-locus variable-number tandem-repeat analysis,MLVA)分型特征,分析其流行病学意义。方法采用MLVA(14+12)分型策略设计引物。利用聚合酶链式反应(PCR)、毛细管电泳方法和Bio Numerics软件,对河北分离的鼠疫菌进行聚类分析。结果在选取的鼠疫菌26个可变数串联重复序列(variable number of tandem repeats,VNTR)位点中,8个VNTR位点对河北省鼠疫菌具有遗传多态性分析意义。河北省鼠疫疫源地分离到的128株鼠疫菌可分为2个群,15个基因型。结论通过MLVA分析显示河北鼠疫菌株具有多态性,基因遗传变异相对稳定,对于未来鼠疫疫情暴发溯源和鼠疫菌种群的研究具有指导意义。展开更多
Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five b...Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hostsIll. In China, brucellosis is a national notifiable communicable disease both in animals and in human. In 2009, 35 816 brucellosis cases were reported. The annual incidence was 2.7 per 100 000 population.展开更多
Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatr...Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro.展开更多
目的建立针对食品来源的单核细胞增生李斯特氏菌(Listeria monocytogenes,Lm)分离株的多位点串联重复序列分型(Multiple-Locus Variable number tandem repeat Analysis,MLVA)方法,为暴发确认和溯源检测提供实验室支持。方法对2005—201...目的建立针对食品来源的单核细胞增生李斯特氏菌(Listeria monocytogenes,Lm)分离株的多位点串联重复序列分型(Multiple-Locus Variable number tandem repeat Analysis,MLVA)方法,为暴发确认和溯源检测提供实验室支持。方法对2005—2014年间分离自食品的91株Lm进行14个可变数目串联重复序列(Variable Number of Tandem Repeats,VNTR)位点的检测,评估最优检测位点组合并分析检测结果。结果通过采用软件分析,由LMV1、LMV2、LMV7、Lm10、Lm11、Lm23、LM-TR6、TR3和Lm15等9个VNTR位点组成的位点组合为最优MLVA检测位点,可以将91株Lm分离株分为70个型别,分型能力达到0.987 1。结论本研究建立的基于全自动毛细管电泳的由9个检测位点组成的Lm的MLVA分型方法,具有操作简便、快速、结果客观、操作标准化、易于在不同实验室间比较的优势,可作为一线检测方法用于李斯特菌病的暴发确认和溯源检测。展开更多
文摘目的研究河北省鼠疫菌株多位点可变数串联重复序列(multiple-locus variable-number tandem-repeat analysis,MLVA)分型特征,分析其流行病学意义。方法采用MLVA(14+12)分型策略设计引物。利用聚合酶链式反应(PCR)、毛细管电泳方法和Bio Numerics软件,对河北分离的鼠疫菌进行聚类分析。结果在选取的鼠疫菌26个可变数串联重复序列(variable number of tandem repeats,VNTR)位点中,8个VNTR位点对河北省鼠疫菌具有遗传多态性分析意义。河北省鼠疫疫源地分离到的128株鼠疫菌可分为2个群,15个基因型。结论通过MLVA分析显示河北鼠疫菌株具有多态性,基因遗传变异相对稳定,对于未来鼠疫疫情暴发溯源和鼠疫菌种群的研究具有指导意义。
文摘Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hostsIll. In China, brucellosis is a national notifiable communicable disease both in animals and in human. In 2009, 35 816 brucellosis cases were reported. The annual incidence was 2.7 per 100 000 population.
基金supported by the National Natural Science Foundation of China[Grant No.81271890]Beijing Municipal Science&Technology Commission Grant[No.Z161100000116088 and Z1711000017081]
文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro.
文摘目的建立针对食品来源的单核细胞增生李斯特氏菌(Listeria monocytogenes,Lm)分离株的多位点串联重复序列分型(Multiple-Locus Variable number tandem repeat Analysis,MLVA)方法,为暴发确认和溯源检测提供实验室支持。方法对2005—2014年间分离自食品的91株Lm进行14个可变数目串联重复序列(Variable Number of Tandem Repeats,VNTR)位点的检测,评估最优检测位点组合并分析检测结果。结果通过采用软件分析,由LMV1、LMV2、LMV7、Lm10、Lm11、Lm23、LM-TR6、TR3和Lm15等9个VNTR位点组成的位点组合为最优MLVA检测位点,可以将91株Lm分离株分为70个型别,分型能力达到0.987 1。结论本研究建立的基于全自动毛细管电泳的由9个检测位点组成的Lm的MLVA分型方法,具有操作简便、快速、结果客观、操作标准化、易于在不同实验室间比较的优势,可作为一线检测方法用于李斯特菌病的暴发确认和溯源检测。
