Objective:To investigate the effect of salvia miltiorrhiza on expression of the MMP-2、9 and TIMP-1、TIMP-2 in tissue of acute lung injury of severe acute pancreatitis(SAP).Methods:MMP-2、9 expression and changes of t...Objective:To investigate the effect of salvia miltiorrhiza on expression of the MMP-2、9 and TIMP-1、TIMP-2 in tissue of acute lung injury of severe acute pancreatitis(SAP).Methods:MMP-2、9 expression and changes of the lung were measured after the SAP rats were induced by retrograde injection of 5%sodium tauocholate into hepatopancreatic duct.The changes of those parameters were also measured after salvia miltiorrhiza was injected intramuscularly just after induction of SAP.Results:The level of MMP-2、9 in pancreas and lung in SAP group were significantly higher than those in sham;The level of MMP-2、9 in salvia miltiorrhiza group were significantly lower than those in SAP group. Conclusion:MMP-2、9 were overexpressed in Acute lung injury (ALI) induced by SAP, salvia miltiorrhiza downregulates MMP-2、9 expression and decreased injury of lung tissue.展开更多
Objective:To explore the influence of charred Gossamer urocteae(CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing.Methods:CGU was extracted with different solve...Objective:To explore the influence of charred Gossamer urocteae(CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing.Methods:CGU was extracted with different solvents and ethanol extract(EE),ethyl acetate fraction(EF),n-butanol fraction(BF) and aqueous fraction(AF) were obtained.The effects of different fractions on the proliferation,matrix metalloproteinase-2,9(MMP-2,9) activities,synthesis of collagen and tissue inhibitor of metalloproteinase 1(TIMP-1) in the mouse oral fibroblasts were determined by MTT,gelatin zymography,chloramine-T method,and enzyme-linked immunosorbent assay(ELISA) respectively.Results:EE,EF and BF at high concentrations could significantly inhibit proliferation of fibroblasts(P<0.05 or P<0.01),and at low concentrations EF and BF could promote proliferation of fibroblasts,and BF and AF could significantly inhibit collagen synthesis(P<0.05 or P<0.01).EE,EF and AF at high concentrations could significantly increase the MMP-9 activity,and BF and AF could significantly inhibit synthesis of TIMP-1.Conclusion:CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen,and in healing of wound,CGU at high concentrations possibly has the functions of anti-fibrosis and anti-scar,and the mechanism to promote degradation of collagen is possibly related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.展开更多
基金supported mainly by Chinese traditionalmedicines and drugs administrative agent(92B093)
文摘Objective:To investigate the effect of salvia miltiorrhiza on expression of the MMP-2、9 and TIMP-1、TIMP-2 in tissue of acute lung injury of severe acute pancreatitis(SAP).Methods:MMP-2、9 expression and changes of the lung were measured after the SAP rats were induced by retrograde injection of 5%sodium tauocholate into hepatopancreatic duct.The changes of those parameters were also measured after salvia miltiorrhiza was injected intramuscularly just after induction of SAP.Results:The level of MMP-2、9 in pancreas and lung in SAP group were significantly higher than those in sham;The level of MMP-2、9 in salvia miltiorrhiza group were significantly lower than those in SAP group. Conclusion:MMP-2、9 were overexpressed in Acute lung injury (ALI) induced by SAP, salvia miltiorrhiza downregulates MMP-2、9 expression and decreased injury of lung tissue.
文摘Objective:To explore the influence of charred Gossamer urocteae(CGU) on the functions of primary cultured mouse oral fibroblasts and reveal its mechanism in wound healing.Methods:CGU was extracted with different solvents and ethanol extract(EE),ethyl acetate fraction(EF),n-butanol fraction(BF) and aqueous fraction(AF) were obtained.The effects of different fractions on the proliferation,matrix metalloproteinase-2,9(MMP-2,9) activities,synthesis of collagen and tissue inhibitor of metalloproteinase 1(TIMP-1) in the mouse oral fibroblasts were determined by MTT,gelatin zymography,chloramine-T method,and enzyme-linked immunosorbent assay(ELISA) respectively.Results:EE,EF and BF at high concentrations could significantly inhibit proliferation of fibroblasts(P<0.05 or P<0.01),and at low concentrations EF and BF could promote proliferation of fibroblasts,and BF and AF could significantly inhibit collagen synthesis(P<0.05 or P<0.01).EE,EF and AF at high concentrations could significantly increase the MMP-9 activity,and BF and AF could significantly inhibit synthesis of TIMP-1.Conclusion:CGU at high concentrations can inhibit the proliferations of fibroblasts and synthesis of collagen,and in healing of wound,CGU at high concentrations possibly has the functions of anti-fibrosis and anti-scar,and the mechanism to promote degradation of collagen is possibly related to the increase in MMP-9 activity and the inhibition of TIMP-1 synthesis.
