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棕色固氮菌缺失nifZ基因的突变种固氮酶MoFe蛋白的纯化和性质 被引量:1
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作者 钟泽璞 胡长征 +3 位作者 王继伟 黄巨富 骆爱玲 李佳格 《Acta Botanica Sinica》 CSCD 1996年第8期605-611,共7页
采用 52℃下加热 6 min,后经 DEAE- 52、Sephacryls S- 2 0 0和 Q- Sepharose等柱层析方法 ,分离纯化了棕色固氮菌 (Azotobacter vinelandii)缺失 nif Z基因突变种固氮酶 Mo Fe(Δnif Z Mo Fe)蛋白 ,其纯度达到电泳纯。Δnif Z Mo Fe蛋... 采用 52℃下加热 6 min,后经 DEAE- 52、Sephacryls S- 2 0 0和 Q- Sepharose等柱层析方法 ,分离纯化了棕色固氮菌 (Azotobacter vinelandii)缺失 nif Z基因突变种固氮酶 Mo Fe(Δnif Z Mo Fe)蛋白 ,其纯度达到电泳纯。Δnif Z Mo Fe蛋白的固氮活性为 2 83nmol C2 H2 还原 / (min·mg蛋白 ) ,远低于野生种 Mo Fe蛋白。Δnif Z Mo Fe蛋白对氧更敏感 ;热稳定性略低于野生种。Δnif Z Mo Fe蛋白的可见光吸收光谱与野生种 Mo Fe蛋白极为相似。其圆二色谱和磁圆二色谱在 450~ 550 nm与野生种 Mo Fe蛋白显著不同 ,表明其 P- cluster及其周围环境与野生种 Mo Fe蛋白有所差异。这亦可能是造成缺失 nif Z突变种 Mo Fe蛋白固氮活性低的原因。 展开更多
关键词 棕色固氮菌 mofe蛋白 固氮酶
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MoFe3S4单立方烷原子簇研究 Ⅳ.新型的双跨桥六配体单立方烷簇合物 MoFe3S4(μ-Me2NCS2)2(Me2NCS2)4·2CH3 CN 的合成与结构 被引量:1
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作者 刘秋田 黄梁仁 +1 位作者 杨瑜 卢嘉锡 《化学学报》 SCIE CAS 1988年第11期1075-1080,共6页
在二甲胺基二硫代甲酸钠、氯化亚铁及四硫代钼酸铵的自兜反应体系中,以分步结晶法分离出MoFe3S4(Me2NCS2)5·CH2Cl2及MoFe3S4(Me2NCS2)6·2CH3CN等单立方烷簇合物,并测定了后者的结构.它的分子含两个Me2NCS2桥,并具有最高核心... 在二甲胺基二硫代甲酸钠、氯化亚铁及四硫代钼酸铵的自兜反应体系中,以分步结晶法分离出MoFe3S4(Me2NCS2)5·CH2Cl2及MoFe3S4(Me2NCS2)6·2CH3CN等单立方烷簇合物,并测定了后者的结构.它的分子含两个Me2NCS2桥,并具有最高核心氧化态[MoFe3S4]6+.本文报道该化合物的结构特点,指出在自兜反应体系中,可能存在几种不同氧化态的单立方烷原子簇化合物. 展开更多
关键词 立方烷 氧化态 Me2NCS2 mofe3S4 原子簇 NCS 配体 簇合物 跨桥 CN CH
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熔炼温度对含钼渣冶炼MoFe中杂质元素含量的影响 被引量:2
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作者 杨双平 周江峰 《有色金属工程》 CAS CSCD 北大核心 2018年第1期57-60,共4页
采用低温冶炼工艺,利用氨浸渣和钼酸钙渣两种含钼工业废渣直接还原冶炼钼铁。研究了熔炼温度对MoFe合金中C、P、S、Si、Cu等杂质元素含量的影响。结果表明,熔炼温度为1 500℃时,MoFe合金中杂质元素含量均符合国标GB/T 3649—2008要求。
关键词 mofe 熔炼温度 杂质元素
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MoFe/HZSM-5催化剂的制备及其性能的研究
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作者 贾太轩 刘自力 +2 位作者 陈胜洲 柯刚 梁红 《功能材料》 EI CAS CSCD 北大核心 2009年第4期625-628,共4页
研究以柠檬酸为络合剂,得到Fe3+、Mo6+的络合液,以分子筛(HZSM-5)为载体,采用浸渍法制备了系列负载型MoFe/HZSM-5催化剂,对二甲苯选择性氧化为探针反应,测定其催化性能。催化剂的活性评价结果表明:金属原子的最佳配比为n(Mo)∶n(Fe)=2.4... 研究以柠檬酸为络合剂,得到Fe3+、Mo6+的络合液,以分子筛(HZSM-5)为载体,采用浸渍法制备了系列负载型MoFe/HZSM-5催化剂,对二甲苯选择性氧化为探针反应,测定其催化性能。催化剂的活性评价结果表明:金属原子的最佳配比为n(Mo)∶n(Fe)=2.4∶1;在600℃焙烧,反应温度为500℃,气体时空速率5500h-1条件下,对苯二甲醛的选择性达到了最大值85.28%。分别用XRD、FT-IR、DSC对MoFe/HZSM-5进行表征,分析谱图得出了一致性结论,MoFe/HZSM-5的主要活性组分为Fe2(MoO4)3;HZSM-5作为载体,提高了对苯二甲醛的选择性,进而加深了对MoFe/HZSM-5微观结构、性质的了解,MoFe/HZSM-5的高活性、选择性,与其微观结构密不可分,为新型催化剂的研制及其工业应用提供了较好的实验依据。 展开更多
关键词 分子筛 mofe/HZSM-5 对二甲苯 选择性氧化
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Studies on Crystalline Growth of MoFe Protein from a nifZ Deleted Strain of Azotobacter vinelandii
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作者 黄巨富 王耀萍 +4 位作者 董志刚 黄孝明 汪道涌 吕玉兵 汪志平 《Acta Botanica Sinica》 CSCD 2000年第4期383-387,共5页
Under a given condition of crystallization, dark brown short rhombohedron crystals could be obtained from Δ nifZ MoFe protein purified from a nifZ deleted mutant strain of Azotobacter vinelandii Lipmann.... Under a given condition of crystallization, dark brown short rhombohedron crystals could be obtained from Δ nifZ MoFe protein purified from a nifZ deleted mutant strain of Azotobacter vinelandii Lipmann. Systematic studies on the effect of concentrations of PEG 8000,MgCl 2, NaCl,Tris and buffer pH on the crystallization and crystal growth of the protein showed that the protein could not be crystallized in lower concentrations of the chemicals and lower buffer pH. A large amount of smaller crystals of the protein appeared in a week with gradual increasing in the chemical concentrations and pH≥8.0. When the chemical concentrations were further increased, the time for crystallization was increased and a few high grade crystals of larger size were formed. If the concentrations of the chemicals were continuously increased, many crystals with smaller size, and, sometimes of poor quality appeared again and eventually ceased to produce any crystals. The optimal concentration for each of the above mentioned chemicals varies with other variable factors. Only one bigger crystal (both of the longest two sides: 0.16 mm) could be obtained in a hanging drop of protein sample when the concentrations of PEG 8000, MgCl 2, NaCl,Tris and protein were kept at 1.86%, 300 mmol/L, 400 mmol/L, 53 mmol/L and 4.64 g/L , respectively, with Tris buffer pH 8.2. 展开更多
关键词 Azotobacter vinelandii nifZ deletion mutant mofe protein crystalline growth
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老年多器官衰竭(MOFE)研究若干进展(讲课提要)
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作者 王士雯 《肾脏病与透析肾移植杂志》 CAS CSCD 1993年第4期322-323,共2页
内分泌和神经系统的变化对MOFE病情变化有着密切相关性近年来,国外对受体系统与衰老关系颇受重视。因β-肾上腺素能受体(β-受体)介导广泛生理效应,放射性标记的特异性配本已被广泛用于各种组织中β-受体测定。近期有人研究了老年人外... 内分泌和神经系统的变化对MOFE病情变化有着密切相关性近年来,国外对受体系统与衰老关系颇受重视。因β-肾上腺素能受体(β-受体)介导广泛生理效应,放射性标记的特异性配本已被广泛用于各种组织中β-受体测定。近期有人研究了老年人外周血淋巴细胞β-肾上腺能受体的变化。结论提示,老年人β-受体β-MAX(最大结合容量)。 展开更多
关键词 老年多器官衰竭 mofe 肾上腺能受体 放射性标记 激动剂 受体β 感染性休克 革兰阴性菌 菌血症 外周血淋巴细胞
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腹膜透析抢救以肾衰为主的MOFE16例报告 被引量:1
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作者 郑烟萍 袁东 +1 位作者 黄玲玲 滕舰 《实用老年医学》 CAS 2002年第4期212-213,共2页
关键词 腹膜透析 抢救 肾衰 mofe 老年多器官功能衰竭
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含MoFe_3S_4单元的μ_2-OR或μ_2-SR桥联双类立方烷簇合物的红外光谱研究
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作者 何玲洁 陈波 +3 位作者 蔡素华 林善伙 陈昌能 张琳娜 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 1999年第5期666-669,共4页
对下列含MoFe3S4 单元的μ2-OR或μ2 -SR桥联双类立方烷簇合物的红外光谱进行了研究:(Et4 N)3[Mo2Fe6 S8Cl6 (OMe)3 ](1),(Et4 N)3 [Mo2 Fe6 S8 (SPh)6 (OMe... 对下列含MoFe3S4 单元的μ2-OR或μ2 -SR桥联双类立方烷簇合物的红外光谱进行了研究:(Et4 N)3[Mo2Fe6 S8Cl6 (OMe)3 ](1),(Et4 N)3 [Mo2 Fe6 S8 (SPh)6 (OMe)3 ](2),(Bu4N)3 [Mo2Fe6S8 (SPh)6 (OMe)3 ](3),(Et4N)3 [Mo2Fe6S8 (SBut)6(OMe)3](4),(Et4N)3 [Mo2Fe6S8 (SPh)9 ](5)。通过这系列簇合物特征振动频率和结构参数及与线型Mo-Fe-S簇合物红外光谱的比较,对其νM o-μ2O(R) ,νM o-μ2S(R),νM o-S,νFe-S,νFe-SR,νFe-Cl 进行了归属。对其中两条途径的亲电诱导效应及Δν(即νM o-S-νFe-S)与Fe→Mo 电荷迁移的关系进行了探讨。最后,讨论了桥联双类立方烷与线型Mo-Fe-S簇合物红外光谱的共性和差异。 展开更多
关键词 红外光谱 桥联 立方烷 双类立方烷 簇合物
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Purification and Activation In Vitro of MoFe Protein from a nifE Deleted Mutant Strain of Azotobacter vinelandii
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作者 赵剑峰 赵颖 +3 位作者 汪志平 吕玉兵 潜中兴 黄巨富 《Acta Botanica Sinica》 CSCD 2003年第7期815-819,共5页
The Deltanif E MoFe protein (Deltanif E Av1) was obtained by a chromatography on DE52, Sephacryl S-300 and Q-Sepharose columns from the unheated crude extract of nifE-deleted mutant strain (DJ35) of Azotobacter vinela... The Deltanif E MoFe protein (Deltanif E Av1) was obtained by a chromatography on DE52, Sephacryl S-300 and Q-Sepharose columns from the unheated crude extract of nifE-deleted mutant strain (DJ35) of Azotobacter vinelandii Lipmann. The analysis by SDS-PAGE showed that the Delta nif EAv1 was similar to OP MoFe protein (Av1) of A. vinelandii in the kinds and molecular weights of subunits (alpha and beta subunit). When complemented with nitrogenase Fe protein (M), the A nif EAv1 had hardly any proton-reduction activity, but could be significantly activated by FeMoco extracted from OP Av1. After the Delta nif E Av1 was treated with an excess o-phenanthroline (o-phen) and chromatographied on Sephadex G-25 column under atmosphere of Ar, Delta nif E Av1(C) was obtained. In the presence of both Av2 and MgATP regeneration system, the Delta nif EAv1(C), rather than A nif EAv1, was significantly activated in vitro by a reconstituent solution containing Mn which composed of KMnO4, ferric homocitrate, Na2S, Na2S2O4 (DT) and dithiothreitol (DTT). But in the absence of MgATP or Av2, the activation of Delta nifE Av1(C) did not happen. It indicates that activation of Delta nif EAv1 by RS-Mn requires the pretreatment with o-phen and the simultaneous presence of Av2 and MgATP. 展开更多
关键词 Delta nif E Av1 PURIFICATION activation and assembly in vitro FeMoco and reconstituent solution containing Mn
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MoFe_3S_4及WFe_3S_4单立方烷簇合物的合成结构及电化学研究 被引量:3
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作者 刘秋田 雷新建 +4 位作者 黄梁仁 陈文章 赵琨 刘汉钦 卢嘉锡 《中国科学(B辑)》 CSCD 北大核心 1990年第6期561-571,共11页
从(NH_4)_2MoS_4或(NH_4)_2WS_4,FeCl_2,R_2NCS_2NH_4(或其钠盐)起始,在DMF中经自兜反应制得了MoFes_3S4(R_2NCS_2)_5(R_2=—(CH_2)_4—,Bz_2)及WFe_3S_4(R_2NCS_2)_5(R_2=Me_2,—(CH_2)_4—)两种单立方烷原子簇。前者也可从链状簇合物F... 从(NH_4)_2MoS_4或(NH_4)_2WS_4,FeCl_2,R_2NCS_2NH_4(或其钠盐)起始,在DMF中经自兜反应制得了MoFes_3S4(R_2NCS_2)_5(R_2=—(CH_2)_4—,Bz_2)及WFe_3S_4(R_2NCS_2)_5(R_2=Me_2,—(CH_2)_4—)两种单立方烷原子簇。前者也可从链状簇合物Fe(DMF)_6[(FeCl_2)_2MoS_4]转化而生成。MoFe_3S_4(C_4H_8NCS_2)_5及WFe_3S_4(C_4H_8NCS_2)_5结构相似,单胞参数几乎相同。在比较它们结构基础上,讨论了这一系列单立方烷原子簇的结构特点,对它们的红外,顺磁谱及电化学性质也进行了研究。 展开更多
关键词 立方烷簇合物 mofe3S4 WFe3S4
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SYNTHESIS, STRUCTURE AND ELECTROCHEMICAL STUDIES ON SINGLE MoFe_3S_4 AND WFe_3S_4 CUBANE-TYPE CLUSTER COMPOUNDS* 被引量:2
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作者 刘秋田 雷新建 +5 位作者 黄梁仁 陈文章 赵琨 陈冬铃 刘汉钦 卢嘉锡 《Science China Chemistry》 SCIE EI CAS 1990年第12期1446-1458,共13页
Single cubane-like clusters, MFe_3S_4(R_2dtc)_5 (M=Mo, R_2=—(CH_2)_4—, Bz_2; M=W, R_2=Me_2, —(CH_2)4—; dtc=dialkyldithiocarbamate) were synthesized by assembly reaction of (NH_4)_2MS_4 (M=Mo, W), FeCl_2 with R_2dt... Single cubane-like clusters, MFe_3S_4(R_2dtc)_5 (M=Mo, R_2=—(CH_2)_4—, Bz_2; M=W, R_2=Me_2, —(CH_2)4—; dtc=dialkyldithiocarbamate) were synthesized by assembly reaction of (NH_4)_2MS_4 (M=Mo, W), FeCl_2 with R_2dtcNa (or NH_4) in DMF. The structure, IR, EPR and cyclic voltammogram are described and discussed. MoFe_3S_4(C_4H_8dtc)_5 was also obtained from the conversion of linear cluster Fe(DMF)_6[(FeCl_2)_2MoS_4] in the presence of C_4H_8dtc NH_4. Both clusters MoFe_3S_4(C_4H_8dtc)_4·2C_2H_5CN and WFe_3S_4(C_4H_8dtc)_5·2DMF crystallize in the monoclinic space group C2/c with a=12.544(2), (Mo-cluster), 12.533(4) (W-cluster); b=20.478(4), 20.121(6); c=19.582(7), 19.659(2);β=102.82(2), 102.96(3)°; and Z=4, 4. Their structures were refined to R=0.057 and 0.064 with use of 2690 and 2179 reflections (ι>3Q(I)), respectively. Many similarities in structure for Mo and W clusters indicate that both Mo and W play a very similar role in the clusters. 展开更多
关键词 mofe_3S_4 cubane cluster WFe_3S_4 cubane cluster mofeS cluster heterometallie cluster heterometallic thiocubane
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固氮酶活性中心研究五十年 被引量:1
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作者 陈全亮 周朝晖 《大学化学》 CAS 2024年第7期287-293,共7页
固氮酶是固氮微生物在常温常压下固氮成氨的催化剂,其活性中心的结构从Fe_(2)S_(2)·Mo_(2)O_(2)演变到MoFe_(7)S_(9)C(RHhomocit)(cys)(his)(H_(4)homocit=高柠檬酸,Hcys=半胱氨酸,Hhis=组氨酸)。本文将回顾这一重要的历史过程,探... 固氮酶是固氮微生物在常温常压下固氮成氨的催化剂,其活性中心的结构从Fe_(2)S_(2)·Mo_(2)O_(2)演变到MoFe_(7)S_(9)C(RHhomocit)(cys)(his)(H_(4)homocit=高柠檬酸,Hcys=半胱氨酸,Hhis=组氨酸)。本文将回顾这一重要的历史过程,探讨化学模拟、光谱学、理论计算和生物化学,特别是结构生物学等领域在固氮酶活性中心研究中的重要成就,从化学结构的角度看固氮酶活性中心研究五十年。 展开更多
关键词 固氮酶 钼铁蛋白 铁钼辅基 活性中心
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Characterization of a FeMo cofactor-deficient MoFe protein from a nifE-deleted strain(DJ35)of Azotobacter vinelandii 被引量:1
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作者 ZHAO Ying BIAN Shaomin +4 位作者 ZHANG Chunxi ZHOU Huina WANG Huangping ZHAO Jianfeng HUANG Jufu 《Chinese Science Bulletin》 SCIE EI CAS 2005年第20期2305-2310,共6页
A MoFe protein (ΔnifE Av1) with a purity of ~80% was purified from a nifE-deleted mutant of Azotobacter vinelandii DJ35. Compared with MoFe protein purified from wild-type strain OP (OP Av1), ΔnifE Av1 had the same... A MoFe protein (ΔnifE Av1) with a purity of ~80% was purified from a nifE-deleted mutant of Azotobacter vinelandii DJ35. Compared with MoFe protein purified from wild-type strain OP (OP Av1), ΔnifE Av1 had the same subunits composition, and had immune reaction with antibody to OP Av1, but its relative mobility in anaerobic native polyacrylamide gel electrophoresis (PAGE) was a little larger than that of OP Av1. Metal analysis showed that Mo and Fe contents of ΔnifE Av1 both apparently decreased. When complemented with OP Fe protein, ΔnifE Av1 had no C2H2-reduction activity, but it could be in vitro activated by FeMoco extracted from OP Av1. The circular dichroism (CD) spectrum of ΔnifE Av1 at ~450 nm was similar to that of OP Av1, while the EPR signal at g≈3.7 was absolutely silent, and the signal intensities at g≈4.3 and 2.0 decreased by 75% and 50%, respectively. The results indicated that ΔnifE Av1 purified from DJ35 was a FeMoco-deficient but P-cluster-con- taining MoFe protein. 展开更多
关键词 mofe蛋白质 固氮细菌 纯净度 ERP 厌氧性
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STUDIES ON SYNTHESES OF MoFe_3S_4 CUBANE-LIKE CLUSTER COMPOUNDS
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作者 刘秋田 康北笙 +4 位作者 陈昌能 黄梁仁 蔡进华 庄伯涛 卢嘉锡 《Science China Chemistry》 SCIE EI CAS 1989年第4期392-400,共9页
The features of synthetic processes are described on the basis of the studies of the syntheses of MoFe_3S_4 cubane-like cluster compounds either by self-assembly reactions or by the conversion of linear clusters. The ... The features of synthetic processes are described on the basis of the studies of the syntheses of MoFe_3S_4 cubane-like cluster compounds either by self-assembly reactions or by the conversion of linear clusters. The redox properties and the structure of ligands as well as the stoichiometry of reactants have obvious effects upon the core oxidation state and the structure of products. It is indicated that the self-assembly reaction may pass through a combination process oflinear cluster intermediates having structural unit (M,M'=Mo) Fe). 展开更多
关键词 Mo CLUSTER Fe CLUSTERS mofeS CLUSTERS HETEROMETALLIC thiocubane.
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Activation In Vitro of Mutant MoFe Proteins from Azotobacter vinelandii by Reconstituent Solutions
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作者 Huang-Ping Wang Ying Zhao +4 位作者 Shao-Min Bian Hui-Na Zhou Zhi-Gang Zhang Jin-Mao Zhu Ju-Fu Huang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第5期567-572,共6页
nifB-MoFe protein (nifB-Av1), AnifE MoFe protein (△nifE Av1) and AnifZ MoFe protein (△nifZ Av1) were obtained by chromatography on DE52, Sephacryl S-300 and Q-Sepharose columns from nifB point-mutated, nifE de... nifB-MoFe protein (nifB-Av1), AnifE MoFe protein (△nifE Av1) and AnifZ MoFe protein (△nifZ Av1) were obtained by chromatography on DE52, Sephacryl S-300 and Q-Sepharose columns from nifB point-mutated, nifE deleted and nifZ deleted mutant stains (UW45, DJ35 and DJ194) of Azotobacter vinelandii Llpmann, respectively. When complemented with nltrogenase Fe protein (Av2), AnifZ Av1 had partial activity and both nifB-Avl and △nifE Av1 had hardly any activity, but could be obviously activated by FeMoco extracted from wild-type MoFe protein (OP Av1) or △nifZ Av1. After being Incubated with excess O-phenanthrollne (O-phen) for 150 mln at 30 ℃ and subjected to chromatography on a Sephadex G-25 column In an Ar atmosphere, nifB- Av1C, △nifE Av1C and △nifZ Av1C were obtained, respectively. Based on a calculation of Fe atoms In the Ophen-Fe compound with ε 512nm = 11 100, lost Fe atoms of nifB-Av1, △nifE Av1 and △nifZ Av1 were estimated to be 1.35, 2.89 and 8.44 per molecule of protein, respectively. As a result of the Fe loss, △nifZ Av1 loses Its original activity. In the presence of both MgATP and Av2, these Fe-loslng proteins, but not the original proteins untreated with O-phen, could be significantly activated by reconstltuent solution (RS) composed of dlthlothreltol, ferric homocltrate, Na2S and Na2MoO4, or K2CrO4, or KMnO4. But In the absence of MgATP or Av2, the activation did not occur, with the exception that △nifZ AvlC was partially activated, and the activity was only 17%. These findings Indicate that: (I) △nifZ Avl with half P-cluster content Is somewhat different from FeMoco-deflclent nifB-Avl and ,△nifE Av1 with respect to protein conformation either before or after treatment with O-phen; (11) full activation of these proteins with RS requires pretreatment with O-phen and the simultaneous presence of MgATP and Av2. 展开更多
关键词 activation in vitro by reconstituent solutions Azotobacter vinelandii mutant mofe protein nifB^- △nifE and △nifZ mutants.
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SYNTHESIS AND STRUCTURE OF A SINGLE MoFe_3S_4 CUBANE-LIKE CLUSTER [MoFe_3S_4(C_5H_(10)NCSS)_5]·CH_2Cl_2
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作者 刘秋田 黄梁仁 +2 位作者 康北笙 杨瑜 卢嘉锡 《Chinese Science Bulletin》 SCIE EI CAS 1987年第13期898-900,共3页
In the study of the catalytic active center of nitrogen fixation in nitrogenase, structural models containing MoFe3S3 or MoFe3S4 cubane-like skeleton have been proposed. The syntheses of these model compounds have sin... In the study of the catalytic active center of nitrogen fixation in nitrogenase, structural models containing MoFe3S3 or MoFe3S4 cubane-like skeleton have been proposed. The syntheses of these model compounds have since attracted the attention of many chemists. Up to the present time, however, only Holm’s research group has obtained MoFe3S4 single cubane cluster compounds by multi-step cleavage reaction of Mo—Fe—S 展开更多
关键词 SKELETON CLEAVAGE fixation CH2CL2 C5H mofe3S4 NCSS BONDS neutral redox
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面向任务的驱逐舰作战效能评估方法研究 被引量:14
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作者 刘旸 孟梅 +1 位作者 张恒 姚军 《哈尔滨工程大学学报》 EI CAS CSCD 北大核心 2013年第12期1479-1489,共11页
为解决驱逐舰面向各种作战任务的效能评估、指标耦合性和评价专家信度问题,提出了面向任务的驱逐舰作战效能评估方法.该方法基于船舶工程分解结构(ship work breakdown structure,SWBS)理论,以驱逐舰作战任务分解清单(required operatio... 为解决驱逐舰面向各种作战任务的效能评估、指标耦合性和评价专家信度问题,提出了面向任务的驱逐舰作战效能评估方法.该方法基于船舶工程分解结构(ship work breakdown structure,SWBS)理论,以驱逐舰作战任务分解清单(required operational capabilities,ROC)为导向,通过评价指标层级度量模式,建立了针对驱逐舰作战效能的耦合评估指标体系,并对该评估指标体系进行了敏感性、耦合性测试.针对评价专家的信度问题,提出信度层次分析法(confidence analytic hierarchy process,CAHP),按照专家权威性和权威领域判定专家在不同置信区间内的置信度.对某型导弹驱逐舰3个设计方案的作战效能进行评估,得到各作战任务下作战效能指数,验证了所提出评估方法的可行性和有效性.研究方法对于各类舰船作战效能评估具有参考价值. 展开更多
关键词 驱逐舰 SWBS—mofe 作战效能评估 性能度量 耦合分析 信度层次分析法
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固氮酶的研究进展 被引量:8
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作者 徐晔 张金池 +1 位作者 王广林 庄家尧 《生物学杂志》 CAS CSCD 2011年第4期61-64,共4页
固氮酶将N2还原为NH3的过程是自然界实现氮循环的重要环节。固氮酶是由γ2型二聚体组成的Fe蛋白和α2β2异四聚体组成的MoFe蛋白组成。固氮酶催化的机制包括铁蛋白的氧还循环和钼铁蛋白的氧还循环两部分。Klebsiella pneumoniae的nif基... 固氮酶将N2还原为NH3的过程是自然界实现氮循环的重要环节。固氮酶是由γ2型二聚体组成的Fe蛋白和α2β2异四聚体组成的MoFe蛋白组成。固氮酶催化的机制包括铁蛋白的氧还循环和钼铁蛋白的氧还循环两部分。Klebsiella pneumoniae的nif基因簇由20个基因组成,构成了8个转录单位,总长度24206bp,其操控机制是多水平、多层次的调控过程。同时综述了固氮酶的多样性,目前已经发现的有钼铁固氮酶、钒铁固氮酶、铁铁固氮酶以及在Strpomyces thermoauophicus内存在的与已知的三种固氮酶体系明显不同的固氮体系。 展开更多
关键词 固氮酶 Fe蛋白 mofe蛋白 nif基因簇
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老年急性脑血管病并发多脏器功能衰竭185例分析 被引量:9
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作者 卫丽 卫萍 王健瑜 《重庆医学》 CAS CSCD 2008年第10期1139-1141,共3页
关键词 老年急性脑血管病 多脏器功能衰竭 并发 器官功能不全综合征 老年多器官衰竭 mofe MODSE 脑血管患者
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老年多脏器功能衰竭病因相关因素研究 被引量:3
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作者 银鹏飞 黄永杰 +3 位作者 孙达 张鸿丽 富晶 马小倩 《北京医学》 CAS 2009年第8期507-507,共1页
老年多脏器功能衰竭(multipleorgan failure in the elderly,MOFE)发病机制复杂,病情凶险,进展迅速,治疗困难,病死率高。现对1998年1月至2008年10月我院480例MOFE患者进行回顾性分析,旨在探讨MOFE发生的相关因素,以期对临床预防和... 老年多脏器功能衰竭(multipleorgan failure in the elderly,MOFE)发病机制复杂,病情凶险,进展迅速,治疗困难,病死率高。现对1998年1月至2008年10月我院480例MOFE患者进行回顾性分析,旨在探讨MOFE发生的相关因素,以期对临床预防和治疗提供参考,报告如下。 展开更多
关键词 多脏器功能衰竭 老年 病因 mofe 发病机制 临床预防 病死率 治疗
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