This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30...This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30%, and 40% respectively - to produce composite materials. In addition, another composite with the same fiber volume is treated with a 4% NaOH solution to improve mechanical properties. The composites are processed by twin-screw extrusion, granulation, and injection molding. Tensile strength measurements of raw fibers and NaOH-treated fibers were carried out using a single-fiber tensile test with a gauge length of 40 mm. It was observed that the NaOH surface treatment increases the resistance against tensile loading and exhibited improved properties for raw fiber strands. The diameter of the fibers was measured using optical microscopy. During this research, flexural tests, impact tests, differential scanning calorimetry (DSC), and heat deflection temperature measurements (HDT) were conducted to evaluate the mechanical and thermal properties of the developed composite samples. The results indicate that the mechanical properties of NaOH-treated Morus alba-reinforced polylactic acid outperform both virgin PLA samples and untreated Morus alba samples.展开更多
Two new prenylflavonoids, momigrol E(1) and mornigrol F(2) were isolated from Morus nigra L. Their structures were elucidated on the basis of spectroscopic methods, especially 2D-NMR techniques.
A new Diels-Alder type adduct mongolicin G (1) and a new flavone 5'-(1", 1"-dimethylallyl)-5,7,2',4'-tetrahydroxyflavone (2) were isolated from the stem and root bark of Morus mongolica. Their structures we...A new Diels-Alder type adduct mongolicin G (1) and a new flavone 5'-(1", 1"-dimethylallyl)-5,7,2',4'-tetrahydroxyflavone (2) were isolated from the stem and root bark of Morus mongolica. Their structures were determined by spectroscopic analysis and chiroptical methods.展开更多
A new alkylene dihydrofuran glycoside(1) was isolated from the root bark ofMorus alba L.,along with moracin M-3′-O-β-D-glucopyranoside (2),and moracin M-6,3′-di-O-β-D-glucopyranoside(3).Compound 1 was identi...A new alkylene dihydrofuran glycoside(1) was isolated from the root bark ofMorus alba L.,along with moracin M-3′-O-β-D-glucopyranoside (2),and moracin M-6,3′-di-O-β-D-glucopyranoside(3).Compound 1 was identified as 2-methylene-3-methoxy-2, 5-dihydrofuran-4-O-β-D-glucopyranoside on the basis of chemical and spectroscopic data including 1D and 2D NMR spectral analysis.In addition,the antioxidant activity of 1 was evaluated using the 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis- 3-ethylbenzothiazoline-6-sulphonic acid(ABTS) assay.The IC_(50) values were 2.49 and 0.45 mg/mL,respectively.展开更多
Mulberry(Morus alba L.)is not only rich in nutrients,but also has medicinal value.As a drug and food,it is widely planted all over the country,with considerable yield and economic value.In recent years,it has attracte...Mulberry(Morus alba L.)is not only rich in nutrients,but also has medicinal value.As a drug and food,it is widely planted all over the country,with considerable yield and economic value.In recent years,it has attracted many scholars and been widely reported,and in-depth research has already been conducted.Through reviewing the literatures on mulberry,this paper summarized the chemical composition,pharmacological effects and quality evaluation of mulberry,providing some reference for the further development and utilization of mulberry.展开更多
Objective: To investigate the possible ameliorative effect of crude water extract of Morus alba(M. alba) leaves on retinopathy of rat pups maternally subjected to diabetes and/or Al intoxication.Methods: Both control ...Objective: To investigate the possible ameliorative effect of crude water extract of Morus alba(M. alba) leaves on retinopathy of rat pups maternally subjected to diabetes and/or Al intoxication.Methods: Both control and experimental groups were subjected to certain integrated approaches, namely, biochemical assessments, light microscopic investigation, transmission electron microscopic investigation, single cell gel electrophoresis(comet assay) and determination of DNA fragmentation.Results: The retina of pups of diabetic and/or Al-intoxicated mothers exhibited abnormal alterations in retinal cell layers including retinal pigmented epithelium, photoreceptor inner segment and ganglion cells. Increased incidence of DNA fragmentation and apoptosis were evident in pups of diabetic and/or Al-intoxicated mothers. However, retina of pups maternally received M. alba extract plus diabetes or Al-intoxicated alone or in combination showed marked amelioration. Less degree of ameliorations was seen in retina of pups maternally subjected to combined treatment. Furthermore, application of crude water extract of M.alba resulted in amelioration of the alterations of maternal serum glucose as well as Al concentration.Conclusions: Based on the results of the present study, M. alba extract is effective against experimentally diabetic and Al-induced developmental retinopathy.展开更多
This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 20...This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 2016-2017,the correlation between Morus alba L. leaf node and its size or mass change was investigated and researched. Results showed that through the correlation coefficient analysis,there was a weakly positive correlation between the leaf node and some indicators such as( leaf length ÷ leaf width) and petiole length,there was a weakly negative correlation between the leaf node and some indicators such as leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass,and there was a weakly negative correlation between the leaf node and the other indicators; there was a highly positive correlation between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width) and( leaf length × leaf width),there was a significantly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in width and petiole mass,and there was a weakly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in thickness. In terms of significant level of difference,it was found that there was no significant difference in the correlation coefficients between the leaf nodes on the Morus alba L. shoots and some indicators such as leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass; there was an extremely significant difference in the correlation coefficients between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),petiole transverse diameter in width and petiole mass; there was no significant difference in the correlation coefficients between the leaf mass and the other indicators. The regression analysis was performed on the leaf node and leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),( leaf length ÷ leaf width),petiole length,petiole diameter in width,petiole diameter in thickness,petiole mass and leaf mass. Significance F values were0. 4864,0. 1995,0. 2888,0. 3004,0. 2350,0. 2407,0. 8925,0. 5227,0. 7022 and 0. 2000,respectively,that is,there was an extremely significant difference between Morus alba L. leaf node and petiole diameter in width,there was a significant difference between Morus alba L.leaf node and petiole mass,and there was no significant difference between Morus alba L. leaf node and the other indicators. The comparative analysis of quadratic curve regression equation and linear regression equation was performed on some indicators such as leaf length,and it was found that the R value between the leaf node and petiole diameter in width was in line with the logarithmic curve regression equation,and the other R values of quadratic curve regression equation was larger than the other R values of linear regression equation,indicating that different Morus alba L. leaf nodes,leaf length,leaf mass and other indicators were in line with the quadratic curve regression equation. It was concluded that by investigating the leaf node on the Morus alba L. shoots and regression equation,we could predict the leaf size,leaf mass and other indicators,and we could take the leaf node on the Morus alba L. shoots as a main factor affecting the Morus alba L. leaf size and mass.展开更多
This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·k...This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.展开更多
White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the pr...White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the present study was to compare chemical composition and antioxidative activity of aqueous extracts prepared from Polish variety of Morus alba leaves at the laboratory (L) and pilot plant scale (PP) conditions. Proximate composition, phenolic acids profile (HPLC/MS), flavonol glicosides (HPLC/ MS), polyphenols (Folin-Ciocalteu assay), and the antioxidant activity (ABTS and DPPH assay) of the extracts were determined. The main phenolic compounds were identified as gallic, protocatechuic, phydroxybenzoic, vanillic, chlorogenic, caffeic, p-coumaric, ferulic, and sinapic acids. Chlorogenic acid was the main phenolic constituent of both extracts. The flavonols fraction contained rutin, quercetin 3-β-D-glucoside, and kaempferol 3-β-D- glucopyranoside. Total concentration of phenolic compounds were 7.9 g and 14.4 g gallic acid equivalent/100 g extract, and antioxidant activity was 137.1 and 214.1 μMol Trolox equivalent/g dry weight for the PP and L extracts, respectively. We concluded that current pilot plant process is less efficient than laboratory process at the aqueous extraction of bioactive components from Morus alba dried leaves. Potential improvements may include increasing efficacy of the extraction, decreasing losses of bioactive components during the process, or both.展开更多
[Objective] The paper was to sequence the rbcL gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splic...[Objective] The paper was to sequence the rbcL gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splicing and correction, a total of 56 rbcL gene sequences were obtained. [Result] Alignment results showed that there were 1 279 permutation sites in rbcL sequence of Morus plants; variable sites accounted for 6.8% of the total sequence length, and the ratio of transition pairs to transversion pairs (R= si/sv) was 1.2. The rbcL sequence of Broussonetia papyrifera was selected as the outgroup, which was downloaded from GenBank. Genetic analysis results showed that M. alba, M. notabilis, M. rubra and Kuisang (the sample of this study) had distant relationship, and the other Morus plants had relatively close relationship. [Conclusion] The results lay a foundation for selection, identification and classification of Morus plants.展开更多
文摘This research investigates the mechanical and thermal properties of Morus alba combined with polylactic acid in comparison with other natural fibers. The study uses three different fiber and PLA compositions - 20%, 30%, and 40% respectively - to produce composite materials. In addition, another composite with the same fiber volume is treated with a 4% NaOH solution to improve mechanical properties. The composites are processed by twin-screw extrusion, granulation, and injection molding. Tensile strength measurements of raw fibers and NaOH-treated fibers were carried out using a single-fiber tensile test with a gauge length of 40 mm. It was observed that the NaOH surface treatment increases the resistance against tensile loading and exhibited improved properties for raw fiber strands. The diameter of the fibers was measured using optical microscopy. During this research, flexural tests, impact tests, differential scanning calorimetry (DSC), and heat deflection temperature measurements (HDT) were conducted to evaluate the mechanical and thermal properties of the developed composite samples. The results indicate that the mechanical properties of NaOH-treated Morus alba-reinforced polylactic acid outperform both virgin PLA samples and untreated Morus alba samples.
基金supported by the National Natural Science Foundation of China(No.20572133).
文摘Two new prenylflavonoids, momigrol E(1) and mornigrol F(2) were isolated from Morus nigra L. Their structures were elucidated on the basis of spectroscopic methods, especially 2D-NMR techniques.
基金the National Natural Science Foundation of China(NO.20432030)for financial support.
文摘A new Diels-Alder type adduct mongolicin G (1) and a new flavone 5'-(1", 1"-dimethylallyl)-5,7,2',4'-tetrahydroxyflavone (2) were isolated from the stem and root bark of Morus mongolica. Their structures were determined by spectroscopic analysis and chiroptical methods.
文摘A new alkylene dihydrofuran glycoside(1) was isolated from the root bark ofMorus alba L.,along with moracin M-3′-O-β-D-glucopyranoside (2),and moracin M-6,3′-di-O-β-D-glucopyranoside(3).Compound 1 was identified as 2-methylene-3-methoxy-2, 5-dihydrofuran-4-O-β-D-glucopyranoside on the basis of chemical and spectroscopic data including 1D and 2D NMR spectral analysis.In addition,the antioxidant activity of 1 was evaluated using the 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis- 3-ethylbenzothiazoline-6-sulphonic acid(ABTS) assay.The IC_(50) values were 2.49 and 0.45 mg/mL,respectively.
基金Supported by Guangxi Natural Science Foundation(2016GXNSFAA380066)Special Fund for Medical Science and Technology of Guangxi Health and Family Planning Commission(GZBZ16-06)+2 种基金Special fund for Construction of Preponderant Disciplines of Guangxi University of Chinese Medicine(ZYX2015004)Construction of the Top Discipline of Guangxi University of Chinese Medicine(2018XK032)Natural Science Research Project of Guangxi University of Chinese Medicine(2015MS004)
文摘Mulberry(Morus alba L.)is not only rich in nutrients,but also has medicinal value.As a drug and food,it is widely planted all over the country,with considerable yield and economic value.In recent years,it has attracted many scholars and been widely reported,and in-depth research has already been conducted.Through reviewing the literatures on mulberry,this paper summarized the chemical composition,pharmacological effects and quality evaluation of mulberry,providing some reference for the further development and utilization of mulberry.
基金Supported by CQAP,Faculty of Science,Menoufiya University(Grant No.CP4-062-Men)
文摘Objective: To investigate the possible ameliorative effect of crude water extract of Morus alba(M. alba) leaves on retinopathy of rat pups maternally subjected to diabetes and/or Al intoxication.Methods: Both control and experimental groups were subjected to certain integrated approaches, namely, biochemical assessments, light microscopic investigation, transmission electron microscopic investigation, single cell gel electrophoresis(comet assay) and determination of DNA fragmentation.Results: The retina of pups of diabetic and/or Al-intoxicated mothers exhibited abnormal alterations in retinal cell layers including retinal pigmented epithelium, photoreceptor inner segment and ganglion cells. Increased incidence of DNA fragmentation and apoptosis were evident in pups of diabetic and/or Al-intoxicated mothers. However, retina of pups maternally received M. alba extract plus diabetes or Al-intoxicated alone or in combination showed marked amelioration. Less degree of ameliorations was seen in retina of pups maternally subjected to combined treatment. Furthermore, application of crude water extract of M.alba resulted in amelioration of the alterations of maternal serum glucose as well as Al concentration.Conclusions: Based on the results of the present study, M. alba extract is effective against experimentally diabetic and Al-induced developmental retinopathy.
基金Supported by Achievement Cultivation and Talent Training Project of Guizhou Academy of Agricultural Sciences[Qian Nong Ke Yuan CR He Zi(2014)52]
文摘This paper aimed to explore the correlation between Morus alba L. leaf node and its size or mass change and provide a theoretical basis for the development of scientific Morus alba L. cultivation technology. During 2016-2017,the correlation between Morus alba L. leaf node and its size or mass change was investigated and researched. Results showed that through the correlation coefficient analysis,there was a weakly positive correlation between the leaf node and some indicators such as( leaf length ÷ leaf width) and petiole length,there was a weakly negative correlation between the leaf node and some indicators such as leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass,and there was a weakly negative correlation between the leaf node and the other indicators; there was a highly positive correlation between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width) and( leaf length × leaf width),there was a significantly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in width and petiole mass,and there was a weakly positive correlation between the leaf mass and some indicators such as petiole transverse diameter in thickness. In terms of significant level of difference,it was found that there was no significant difference in the correlation coefficients between the leaf nodes on the Morus alba L. shoots and some indicators such as leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width) and leaf mass; there was an extremely significant difference in the correlation coefficients between the leaf mass and some indicators such as Morus alba L. leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),petiole transverse diameter in width and petiole mass; there was no significant difference in the correlation coefficients between the leaf mass and the other indicators. The regression analysis was performed on the leaf node and leaf length,leaf width,( leaf length + leaf width),( leaf length × leaf width),( leaf length ÷ leaf width),petiole length,petiole diameter in width,petiole diameter in thickness,petiole mass and leaf mass. Significance F values were0. 4864,0. 1995,0. 2888,0. 3004,0. 2350,0. 2407,0. 8925,0. 5227,0. 7022 and 0. 2000,respectively,that is,there was an extremely significant difference between Morus alba L. leaf node and petiole diameter in width,there was a significant difference between Morus alba L.leaf node and petiole mass,and there was no significant difference between Morus alba L. leaf node and the other indicators. The comparative analysis of quadratic curve regression equation and linear regression equation was performed on some indicators such as leaf length,and it was found that the R value between the leaf node and petiole diameter in width was in line with the logarithmic curve regression equation,and the other R values of quadratic curve regression equation was larger than the other R values of linear regression equation,indicating that different Morus alba L. leaf nodes,leaf length,leaf mass and other indicators were in line with the quadratic curve regression equation. It was concluded that by investigating the leaf node on the Morus alba L. shoots and regression equation,we could predict the leaf size,leaf mass and other indicators,and we could take the leaf node on the Morus alba L. shoots as a main factor affecting the Morus alba L. leaf size and mass.
文摘This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.
文摘White mulberry tree (Morus alba L) is cultivated throughout Asia and Europe, including Poland. The leaves and root bark preparations from Morus alba have been used in traditional phytomedicine. The objective of the present study was to compare chemical composition and antioxidative activity of aqueous extracts prepared from Polish variety of Morus alba leaves at the laboratory (L) and pilot plant scale (PP) conditions. Proximate composition, phenolic acids profile (HPLC/MS), flavonol glicosides (HPLC/ MS), polyphenols (Folin-Ciocalteu assay), and the antioxidant activity (ABTS and DPPH assay) of the extracts were determined. The main phenolic compounds were identified as gallic, protocatechuic, phydroxybenzoic, vanillic, chlorogenic, caffeic, p-coumaric, ferulic, and sinapic acids. Chlorogenic acid was the main phenolic constituent of both extracts. The flavonols fraction contained rutin, quercetin 3-β-D-glucoside, and kaempferol 3-β-D- glucopyranoside. Total concentration of phenolic compounds were 7.9 g and 14.4 g gallic acid equivalent/100 g extract, and antioxidant activity was 137.1 and 214.1 μMol Trolox equivalent/g dry weight for the PP and L extracts, respectively. We concluded that current pilot plant process is less efficient than laboratory process at the aqueous extraction of bioactive components from Morus alba dried leaves. Potential improvements may include increasing efficacy of the extraction, decreasing losses of bioactive components during the process, or both.
基金Supported by Basic and Frontier Research Project of Chongqing City(cstc2015jcyj A80010)Basic Scientific Research Expense Project of Chongqing City(16441)Agricultural Finance Project of Chongqing Agricultural Committee(15403)
文摘[Objective] The paper was to sequence the rbcL gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splicing and correction, a total of 56 rbcL gene sequences were obtained. [Result] Alignment results showed that there were 1 279 permutation sites in rbcL sequence of Morus plants; variable sites accounted for 6.8% of the total sequence length, and the ratio of transition pairs to transversion pairs (R= si/sv) was 1.2. The rbcL sequence of Broussonetia papyrifera was selected as the outgroup, which was downloaded from GenBank. Genetic analysis results showed that M. alba, M. notabilis, M. rubra and Kuisang (the sample of this study) had distant relationship, and the other Morus plants had relatively close relationship. [Conclusion] The results lay a foundation for selection, identification and classification of Morus plants.