【目的】探究中华鳖(Pelodiscus sinensis)甘露糖受体C2(Mannose receptor C type 2)基因PsMRC2在应答嗜水气单胞菌(Aeromonas hydrophila)感染中的免疫功能,为中华鳖抗细菌感染的免疫机制研究提供理论依据。【方法】通过PCR克隆PsMRC2...【目的】探究中华鳖(Pelodiscus sinensis)甘露糖受体C2(Mannose receptor C type 2)基因PsMRC2在应答嗜水气单胞菌(Aeromonas hydrophila)感染中的免疫功能,为中华鳖抗细菌感染的免疫机制研究提供理论依据。【方法】通过PCR克隆PsMRC2基因开放阅读框(ORF)序列,对其进行生物信息学和系统发育进化分析,并以实时荧光定量PCR检测PsMRC2基因在中华鳖各组织中表达谱及嗜水气单胞菌感染后的时序表达。【结果】PsMRC2基因ORF序列为4086 bp,共编码1361个氨基酸残基。PsMRC2蛋白结构域包括1个纤连蛋白Ⅱ型(FNⅡ)结构域(第61~109位氨基酸)、8个串联的C型凝集素(CLECT)结构域(第116~1276位氨基酸)、1个跨膜结构域(第1295~1317位氨基酸)。通过SOMPA预测得到PsMRC2蛋白二级结构包含706个无规则卷曲(占51.87%)、302个延伸链(占22.19%)、289个α-螺旋(占21.23%)和64个β-转角(占4.70%);SWISS-MODEL预测PsMRC2蛋白三级结构匹配的模板与PsMRC2氨基酸序列相似性为90%,预测结果可信。基于PsMRC2蛋白氨基酸序列相似性构建的系统发育进化树显示,中华鳖与龟类的亲缘关系最近,而与鱼类相距较远。PsMRC2基因在中华鳖的心脏、肝脏、脾脏、肾脏、肠道、肌肉、脑和肺脏组织中均有表达,以脾脏组织中的相对表达量最高。感染嗜水气单胞菌后,PsMRC2基因在中华鳖肝脏、脾脏、肾脏和肠道组织中的相对表达量均呈上调趋势。【结论】PsMRC2基因在进化上较保守,广泛表达于中华鳖各组织中,参与中华鳖抗细菌感染的免疫应答过程。展开更多
目的:探究在替牙列期,对深覆牙合的患儿行MRC(Myofunctional research company)早期矫治后,其软组织及硬组织的变化,评价MRC肌功能训练器对于深覆牙合病例的早期治疗效果,以期为深覆牙合病例的早期矫治提供依据。方法:回顾性分析2019年1...目的:探究在替牙列期,对深覆牙合的患儿行MRC(Myofunctional research company)早期矫治后,其软组织及硬组织的变化,评价MRC肌功能训练器对于深覆牙合病例的早期治疗效果,以期为深覆牙合病例的早期矫治提供依据。方法:回顾性分析2019年12月-2020年8月于苏州大学附属口腔医院就诊的50例替牙期中重度牙性深覆牙合患儿的临床资料,早期应用MRC肌功能训练器,比较其矫治前后硬组织及软组织变化,分析其前后改变的相关性。结果:治疗前后软组织测量值Z角,上唇E线距,下唇E线距差异无统计学意义(P>0.05),治疗前后硬组织测量值SNA、ANB、NP-FH、NA-PA、MP-FH、Y轴角、U1-L1、U1-NA、U1-NA、L1-MP、Po-NB距、FMA(FH-MP)差异均无统计学意义(P>0.05);治疗后,SNB、L1-NB、L1-NB均大于治疗前,而覆牙合小于治疗前,差异均有统计学意义(P<0.05)。结论:MRC肌功能训练器可显著减小替牙期患儿的中重度前牙深覆牙合,同时可以增加下前牙唇倾度,改善下颌后缩。展开更多
The Mixed Refrigerant(MR)component is an important factor influencing the performances of natural gas lique-faction processes.However,there is a lack of systematic research about the utilization of propane pre-cooled(...The Mixed Refrigerant(MR)component is an important factor influencing the performances of natural gas lique-faction processes.However,there is a lack of systematic research about the utilization of propane pre-cooled(C3/MRC).In this paper,this mixed refrigerant cycle liquefaction process is simulated using the HYSYS software and the main influential parameters involved in the process are varied to analyze their influence on the liquefaction rate and power consumption.The results show that an effective way for lowering the power consumption of the compressor consists of reducing the flow through the compressor through optimization of the percentage of mixed refrigerant.The power consumption of the compressor in the hybrid refrigeration process is affected by both flow and pressure ratios.Its specific power consumption can be reduced by increasing the flow and decreasing the pressure ratio at the same time.The increase in refrigerant pressure at the high-pressure end can significantly mitigate the energy loss of the heat exchanger and compressor.展开更多
In the natural gas liquefaction process,the mixed refrigerant natural gas liquefaction process is widely used in LNG liquefaction plants because of its advantages of low energy consumption.This paper focuses on the in...In the natural gas liquefaction process,the mixed refrigerant natural gas liquefaction process is widely used in LNG liquefaction plants because of its advantages of low energy consumption.This paper focuses on the influences of important parameters in the C3/MRC liquefaction process,that is,the comparison between propane precooling temperature and the number of moles of methane in mixed refrigerant,power consumption and loss.In addition,the total process was optimized with the optimizer and manual adjustment in HYSYS software to minimize the total power consumption.The results show that with increasing propane precooling temperature,the propane flow rate is almost unchanged,while the mixed refrigerant flow rate decreases significantly,and the loss of the heat exchanger increases significantly.The power consumption of the propane precooling cycle and hybrid refrigeration cycle increases with increasing methane content in the refrigerant,so the power consumption of the whole process increases accordingly.The effect of the methane content in the mixed refrigerant on the process evaluation index is more significant than that of the propane precooling temperature.展开更多
文摘【目的】探究中华鳖(Pelodiscus sinensis)甘露糖受体C2(Mannose receptor C type 2)基因PsMRC2在应答嗜水气单胞菌(Aeromonas hydrophila)感染中的免疫功能,为中华鳖抗细菌感染的免疫机制研究提供理论依据。【方法】通过PCR克隆PsMRC2基因开放阅读框(ORF)序列,对其进行生物信息学和系统发育进化分析,并以实时荧光定量PCR检测PsMRC2基因在中华鳖各组织中表达谱及嗜水气单胞菌感染后的时序表达。【结果】PsMRC2基因ORF序列为4086 bp,共编码1361个氨基酸残基。PsMRC2蛋白结构域包括1个纤连蛋白Ⅱ型(FNⅡ)结构域(第61~109位氨基酸)、8个串联的C型凝集素(CLECT)结构域(第116~1276位氨基酸)、1个跨膜结构域(第1295~1317位氨基酸)。通过SOMPA预测得到PsMRC2蛋白二级结构包含706个无规则卷曲(占51.87%)、302个延伸链(占22.19%)、289个α-螺旋(占21.23%)和64个β-转角(占4.70%);SWISS-MODEL预测PsMRC2蛋白三级结构匹配的模板与PsMRC2氨基酸序列相似性为90%,预测结果可信。基于PsMRC2蛋白氨基酸序列相似性构建的系统发育进化树显示,中华鳖与龟类的亲缘关系最近,而与鱼类相距较远。PsMRC2基因在中华鳖的心脏、肝脏、脾脏、肾脏、肠道、肌肉、脑和肺脏组织中均有表达,以脾脏组织中的相对表达量最高。感染嗜水气单胞菌后,PsMRC2基因在中华鳖肝脏、脾脏、肾脏和肠道组织中的相对表达量均呈上调趋势。【结论】PsMRC2基因在进化上较保守,广泛表达于中华鳖各组织中,参与中华鳖抗细菌感染的免疫应答过程。
文摘目的:探究在替牙列期,对深覆牙合的患儿行MRC(Myofunctional research company)早期矫治后,其软组织及硬组织的变化,评价MRC肌功能训练器对于深覆牙合病例的早期治疗效果,以期为深覆牙合病例的早期矫治提供依据。方法:回顾性分析2019年12月-2020年8月于苏州大学附属口腔医院就诊的50例替牙期中重度牙性深覆牙合患儿的临床资料,早期应用MRC肌功能训练器,比较其矫治前后硬组织及软组织变化,分析其前后改变的相关性。结果:治疗前后软组织测量值Z角,上唇E线距,下唇E线距差异无统计学意义(P>0.05),治疗前后硬组织测量值SNA、ANB、NP-FH、NA-PA、MP-FH、Y轴角、U1-L1、U1-NA、U1-NA、L1-MP、Po-NB距、FMA(FH-MP)差异均无统计学意义(P>0.05);治疗后,SNB、L1-NB、L1-NB均大于治疗前,而覆牙合小于治疗前,差异均有统计学意义(P<0.05)。结论:MRC肌功能训练器可显著减小替牙期患儿的中重度前牙深覆牙合,同时可以增加下前牙唇倾度,改善下颌后缩。
基金supported by the Science Development Funding Program of Dongying of China(Grant No.DJ2021006)Science Development Funding Program of Dongying of China(Grant No.DJ2021008).
文摘The Mixed Refrigerant(MR)component is an important factor influencing the performances of natural gas lique-faction processes.However,there is a lack of systematic research about the utilization of propane pre-cooled(C3/MRC).In this paper,this mixed refrigerant cycle liquefaction process is simulated using the HYSYS software and the main influential parameters involved in the process are varied to analyze their influence on the liquefaction rate and power consumption.The results show that an effective way for lowering the power consumption of the compressor consists of reducing the flow through the compressor through optimization of the percentage of mixed refrigerant.The power consumption of the compressor in the hybrid refrigeration process is affected by both flow and pressure ratios.Its specific power consumption can be reduced by increasing the flow and decreasing the pressure ratio at the same time.The increase in refrigerant pressure at the high-pressure end can significantly mitigate the energy loss of the heat exchanger and compressor.
基金supported by the Research Project of Sinopec Shengli Oilfield Company“Research of Energy Flow Optimization Analysis and Application Technology of Oilfield Production System”.
文摘In the natural gas liquefaction process,the mixed refrigerant natural gas liquefaction process is widely used in LNG liquefaction plants because of its advantages of low energy consumption.This paper focuses on the influences of important parameters in the C3/MRC liquefaction process,that is,the comparison between propane precooling temperature and the number of moles of methane in mixed refrigerant,power consumption and loss.In addition,the total process was optimized with the optimizer and manual adjustment in HYSYS software to minimize the total power consumption.The results show that with increasing propane precooling temperature,the propane flow rate is almost unchanged,while the mixed refrigerant flow rate decreases significantly,and the loss of the heat exchanger increases significantly.The power consumption of the propane precooling cycle and hybrid refrigeration cycle increases with increasing methane content in the refrigerant,so the power consumption of the whole process increases accordingly.The effect of the methane content in the mixed refrigerant on the process evaluation index is more significant than that of the propane precooling temperature.
