IPv6协议一致性测试是检验IPv6协议实现是否正确的重要方法。为弥补我国在该领域研究水平的不足,文中提出了一种模块化测试描述模型,设计了模块化测试描述语言(Modular Test Description Language,MTDL),并开发了基于该模型和语言的IPv...IPv6协议一致性测试是检验IPv6协议实现是否正确的重要方法。为弥补我国在该领域研究水平的不足,文中提出了一种模块化测试描述模型,设计了模块化测试描述语言(Modular Test Description Language,MTDL),并开发了基于该模型和语言的IPv6协议一致性测试系统。在对IPv6协议标准深入分析的基础上设计了一套测试集,用自主开发的测试系统在Linux系统平台上对IPv6协议进行了测试,得到了预期结果,验证了MTDL语言的正确性和有效性。展开更多
Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1(mtd1), a stable multi-tiller and dwarf mutant, was screen...Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1(mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date.By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650(BC12) in mtd1 mutant had a single nucleotide substitution(G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant.Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid(GA)treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.展开更多
基金Acknowledgments The authors acknowledge the financial support of the Natural Science Foundation of Guangxi Province （No. 2013GXNSFAA019019） and the Natural Science Foundation of Guangxi Province （No. 2013GXNSFAA019041）.
文摘IPv6协议一致性测试是检验IPv6协议实现是否正确的重要方法。为弥补我国在该领域研究水平的不足,文中提出了一种模块化测试描述模型,设计了模块化测试描述语言(Modular Test Description Language,MTDL),并开发了基于该模型和语言的IPv6协议一致性测试系统。在对IPv6协议标准深入分析的基础上设计了一套测试集,用自主开发的测试系统在Linux系统平台上对IPv6协议进行了测试,得到了预期结果,验证了MTDL语言的正确性和有效性。
基金supported by the National Natural Science Foundation of China (31401464, 31201183)Zhejiang Provincial Natural Science Foundation of China (Y3110194, LY16C130001)+1 种基金China Postdoctoral Science Foundation (2014M561108)the Open Foundation from Zhejiang Provincial Top Key Discipline of Biology (KFJJ2014006)
文摘Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1(mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date.By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650(BC12) in mtd1 mutant had a single nucleotide substitution(G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant.Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid(GA)treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
