Objective:To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe(仙鹤延龄方),a recipe commonly used in cancer patients in China.Methods: The in vitro an...Objective:To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe(仙鹤延龄方),a recipe commonly used in cancer patients in China.Methods: The in vitro anticancer activity of the total flavonoids was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay on three cancer cell lines:MCF-7(a human breast adenocarcinoma cell line),HepG-2(a human hepatocellular carcinoma cell line) and ES-2(a human ovarian cancer cell line). The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma,and cisplatin was used as a positive control.Results:The total flavonoids exerted a powerful inhibitory effect on the three cell lines,with 50%inhibiting concentrations(IC_(50)) of 24.948,31.569 and 6.923μg/mL,respectively.In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.Conclusion:The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity,and further researches and development are warranted.展开更多
文摘目的:观察内环境因素(pH和甘氨酸及离子浓度)对依达拉奉抗神经元缺血性损伤作用的影响。方法:大鼠原代培养皮质神经元,在不同的实验溶液(模拟缺血后脑内环境的变化)以缺氧缺糖(oxygen-g lucose deprivation,OGD)3 h和再灌12 h诱导皮质神经元损伤,以噻唑蓝(MTT)还原反应和乳酸脱氢酶(LDH)释放观察损伤变化。观察不同实验溶液对抗脑缺血药物依达拉奉抗缺血性损伤作用的影响。结果:在弱碱性(pH 7.8)或含甘氨酸(10μm o l/L)的实验溶液中,OGD损伤加重;在弱酸性(pH 6.5)或高M g2+(1.8 mm o l/L)条件下,OGD损伤减轻。依达拉奉(1μm o l/L)能逆转在pH 6.1、7.4、7.8或含甘氨酸溶液中OGD损伤;而对pH 6.5、高M g2+或低Ca2+溶液中的OGD损伤未显示保护作用。结论:内环境因素改变可影响缺血性损伤程度和依达拉奉抗损伤作用。
文摘Objective:To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe(仙鹤延龄方),a recipe commonly used in cancer patients in China.Methods: The in vitro anticancer activity of the total flavonoids was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay on three cancer cell lines:MCF-7(a human breast adenocarcinoma cell line),HepG-2(a human hepatocellular carcinoma cell line) and ES-2(a human ovarian cancer cell line). The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma,and cisplatin was used as a positive control.Results:The total flavonoids exerted a powerful inhibitory effect on the three cell lines,with 50%inhibiting concentrations(IC_(50)) of 24.948,31.569 and 6.923μg/mL,respectively.In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.Conclusion:The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity,and further researches and development are warranted.