目的:研究肌球蛋白轻链(MYL9)在非小细胞肺癌(NSCLC)患者的淋巴结和外周血中的表达及其与临床病理特征的关系。方法:选取在2015年4月至2016年10月广西医科大学第一附属医院胸外科,并行肺叶切除+系统性淋巴结清扫手术治疗的60例NSCLC患...目的:研究肌球蛋白轻链(MYL9)在非小细胞肺癌(NSCLC)患者的淋巴结和外周血中的表达及其与临床病理特征的关系。方法:选取在2015年4月至2016年10月广西医科大学第一附属医院胸外科,并行肺叶切除+系统性淋巴结清扫手术治疗的60例NSCLC患者作为研究组,同期选取60例无恶性肿瘤病史的健康体检者为对照组。采用实时荧光定量PCR(q PCR)及western blotting法检测MYL9在淋巴结和外周血中m RNA和蛋白表达水平;多因素Logistic回归模型分析MYL9m RNA及蛋白表达的影响因素。结果:研究组外周血MYL9 m RNA及蛋白表达量均高于对照组(均P<0.05);MYL9 m RNA和蛋白在淋巴结转移阳性患者的表达量均高于淋巴结转移阴性患者的表达量,差异有统计学意义(均P<0.05);经多因素Logistic回归分析,肿瘤分化程度和淋巴结分期是影响NSCLC患者MYL9表达的独立危险因素(P<0.05)。结论:NSCLC患者肿瘤分化程度越差,淋巴结分期越晚,MYL9 m RNA和蛋白表达上调越明显;MYL9可能参与了NSCLC的转移过程,是一个潜在的预后预测指标。展开更多
Atrial fibrillation(AF)is a common cardiac disease with high prevalence in the general population.Despite a mild manifestation at the onset stage,it causes serious consequences,including sudden death,when the disease ...Atrial fibrillation(AF)is a common cardiac disease with high prevalence in the general population.Despite a mild manifestation at the onset stage,it causes serious consequences,including sudden death,when the disease progresses to the late stage.Most available treatments of AF focus on symptom management or alleviation,due to a lack of fundamental knowledge and the fact that considerable variations of AF exist.With the popularisation of the next-generation sequencing technology,several causal genetic factors,including MYL4,have been discovered to contribute to AF,giving hope to developing its gene therapies.In this study,we attempted to treat a previously established rat AF model,which carried Myl4E11K/E11K loss of function mutation,via overexpression of exogenous wild-type Myl4 by AAV9 vectors.Our results showed that delivery of Myl4 expressing AAV9 to postnatal rat models rescued the symptoms of AF,indicating the therapeutic potential that early gene therapy intervention can achieve long-term effects in treating cardiac arrhythmias caused by gene mutations.展开更多
文摘目的:研究肌球蛋白轻链(MYL9)在非小细胞肺癌(NSCLC)患者的淋巴结和外周血中的表达及其与临床病理特征的关系。方法:选取在2015年4月至2016年10月广西医科大学第一附属医院胸外科,并行肺叶切除+系统性淋巴结清扫手术治疗的60例NSCLC患者作为研究组,同期选取60例无恶性肿瘤病史的健康体检者为对照组。采用实时荧光定量PCR(q PCR)及western blotting法检测MYL9在淋巴结和外周血中m RNA和蛋白表达水平;多因素Logistic回归模型分析MYL9m RNA及蛋白表达的影响因素。结果:研究组外周血MYL9 m RNA及蛋白表达量均高于对照组(均P<0.05);MYL9 m RNA和蛋白在淋巴结转移阳性患者的表达量均高于淋巴结转移阴性患者的表达量,差异有统计学意义(均P<0.05);经多因素Logistic回归分析,肿瘤分化程度和淋巴结分期是影响NSCLC患者MYL9表达的独立危险因素(P<0.05)。结论:NSCLC患者肿瘤分化程度越差,淋巴结分期越晚,MYL9 m RNA和蛋白表达上调越明显;MYL9可能参与了NSCLC的转移过程,是一个潜在的预后预测指标。
基金ECNU Public Platform for innovation(011)grants from National Key R&D Program of China(2019YFA0110802 and 2019YFA0802802)+3 种基金National Science and Technology Major Project(2019ZX09301-132)the National Natural Science Foundation of China(81873685,31971366,32101194,32025023)the Shanghai Municipal Commission for Science and Technology(18411953500)a grant from Innovation program of Shanghai Municipal Education Commission(2019-01-07-00-05-E00054).
文摘Atrial fibrillation(AF)is a common cardiac disease with high prevalence in the general population.Despite a mild manifestation at the onset stage,it causes serious consequences,including sudden death,when the disease progresses to the late stage.Most available treatments of AF focus on symptom management or alleviation,due to a lack of fundamental knowledge and the fact that considerable variations of AF exist.With the popularisation of the next-generation sequencing technology,several causal genetic factors,including MYL4,have been discovered to contribute to AF,giving hope to developing its gene therapies.In this study,we attempted to treat a previously established rat AF model,which carried Myl4E11K/E11K loss of function mutation,via overexpression of exogenous wild-type Myl4 by AAV9 vectors.Our results showed that delivery of Myl4 expressing AAV9 to postnatal rat models rescued the symptoms of AF,indicating the therapeutic potential that early gene therapy intervention can achieve long-term effects in treating cardiac arrhythmias caused by gene mutations.
