目的建立脂多糖(LPS)介导的大鼠急性肺损伤后早期肺纤维化模型并观察其过程中血管紧张素系统主要组分血管紧张素Ⅱ1型受体(AT1R)和Mas受体的变化趋势。方法分别由腹腔和气道内间断3次注射LPS建立大鼠急性肺损伤后早期肺纤维化模型,并于...目的建立脂多糖(LPS)介导的大鼠急性肺损伤后早期肺纤维化模型并观察其过程中血管紧张素系统主要组分血管紧张素Ⅱ1型受体(AT1R)和Mas受体的变化趋势。方法分别由腹腔和气道内间断3次注射LPS建立大鼠急性肺损伤后早期肺纤维化模型,并于末次注射后第3、7、14和21日处死大鼠,取肺组织及血浆。H-E染色和Masson染色观察肺组织形态结构改变;ELISA试剂盒检测血浆中TGF-β的水平;RT-q PCR及Western blotting检测肺组织中AT1R和Mas m RNA和蛋白表达变化。结果经LPS复合打击后,大鼠肺组织病理改变在第3日以肺部炎症反应为主要表现,而第7日后炎症减弱,肺组织正常形态结构遭到破坏,Masson染色提示胶原纤维沉积逐渐增加,血浆中TGF-β的含量逐渐升高(P<0.01),从而形成早期肺纤维化。另外,随着LPS诱导肺纤维化的发展,AT1R m RNA表达无显著变化,但其蛋白表达在第14日及21日明显增加;肺组织内Mas m RNA表达逐渐增加,至第21日明显升高至对照组的15倍,而Mas受体蛋白的表达在第14日及21日明显下调至对照组的30%(P<0.01)。结论 LPS复合多次打击能有效建立大鼠急性肺损伤后早期肺纤维化模型,且随着肺纤维化发展,AT1R/Mas受体呈上升/下降相反的表达变化。展开更多
Invasive alien species are primary drivers of biodiversity loss and species extinction.Smooth cordgrass(Spartina alterniflora)is one of the most aggressive invasive plants in coastal ecosystems around the world.Howeve...Invasive alien species are primary drivers of biodiversity loss and species extinction.Smooth cordgrass(Spartina alterniflora)is one of the most aggressive invasive plants in coastal ecosystems around the world.However,the genomic bases and evolutionary mechanisms underlying its invasion success have remained largely unknown.Here,we assembled a chromosome-level reference genome and performed phenotypic and population genomic analyses between native US and introduced Chinese populations.Our phenotypic comparisons showed that introduced Chinese populations have evolved competitive traits,such as earlyflowering time and greater plant biomass,during secondary introductions along China’s coast.Population genomic and transcriptomic inferences revealed distinct evolutionary trajectories of low-and high-latitude Chinese populations.In particular,genetic mixture among different source populations,together with in-dependent natural selection acting on distinct target genes,may have resulted in high genome dynamics of the introduced Chinese populations.Our study provides novel phenotypic and genomic evidence showing how smooth cordgrass rapidly adapts to variable environmental conditions in its introduced ranges.Moreover,candidate genes related toflowering time,fast growth,and stress tolerance(i.e.,salinity and submergence)provide valuable genetic resources for future improvement of cereal crops.展开更多
对原产湖北五峰的红花玉兰新变种多瓣红花玉兰(Magnolia wufengensisvar.multitepalaL.Y.Ma et L.R.W ang)作了描述。新变种与红花玉兰原变种(M.wufengensisvar.wufengensis)花色相近,整个花被片内外均为红色,内面略淡。主要区别在于:...对原产湖北五峰的红花玉兰新变种多瓣红花玉兰(Magnolia wufengensisvar.multitepalaL.Y.Ma et L.R.W ang)作了描述。新变种与红花玉兰原变种(M.wufengensisvar.wufengensis)花色相近,整个花被片内外均为红色,内面略淡。主要区别在于:新变种花被片12、15、18、24;花被片阔倒卵状匙形、倒卵状匙形或窄倒卵状匙形;花色浓淡不同植株间有深红、红、浅红之差别;自然状态下可育,蓇葖果发育良好。展开更多
文摘目的建立脂多糖(LPS)介导的大鼠急性肺损伤后早期肺纤维化模型并观察其过程中血管紧张素系统主要组分血管紧张素Ⅱ1型受体(AT1R)和Mas受体的变化趋势。方法分别由腹腔和气道内间断3次注射LPS建立大鼠急性肺损伤后早期肺纤维化模型,并于末次注射后第3、7、14和21日处死大鼠,取肺组织及血浆。H-E染色和Masson染色观察肺组织形态结构改变;ELISA试剂盒检测血浆中TGF-β的水平;RT-q PCR及Western blotting检测肺组织中AT1R和Mas m RNA和蛋白表达变化。结果经LPS复合打击后,大鼠肺组织病理改变在第3日以肺部炎症反应为主要表现,而第7日后炎症减弱,肺组织正常形态结构遭到破坏,Masson染色提示胶原纤维沉积逐渐增加,血浆中TGF-β的含量逐渐升高(P<0.01),从而形成早期肺纤维化。另外,随着LPS诱导肺纤维化的发展,AT1R m RNA表达无显著变化,但其蛋白表达在第14日及21日明显增加;肺组织内Mas m RNA表达逐渐增加,至第21日明显升高至对照组的15倍,而Mas受体蛋白的表达在第14日及21日明显下调至对照组的30%(P<0.01)。结论 LPS复合多次打击能有效建立大鼠急性肺损伤后早期肺纤维化模型,且随着肺纤维化发展,AT1R/Mas受体呈上升/下降相反的表达变化。
基金supported by the National Key Research and Development Program of China (2022YFC2601100 to B.L.)the Natural Science Foundation of China (32030067 to J.-H.W.,31970235 to L.-F.L.,32171661 to R.-T.J.,and 31961133028 to B.L.).
文摘Invasive alien species are primary drivers of biodiversity loss and species extinction.Smooth cordgrass(Spartina alterniflora)is one of the most aggressive invasive plants in coastal ecosystems around the world.However,the genomic bases and evolutionary mechanisms underlying its invasion success have remained largely unknown.Here,we assembled a chromosome-level reference genome and performed phenotypic and population genomic analyses between native US and introduced Chinese populations.Our phenotypic comparisons showed that introduced Chinese populations have evolved competitive traits,such as earlyflowering time and greater plant biomass,during secondary introductions along China’s coast.Population genomic and transcriptomic inferences revealed distinct evolutionary trajectories of low-and high-latitude Chinese populations.In particular,genetic mixture among different source populations,together with in-dependent natural selection acting on distinct target genes,may have resulted in high genome dynamics of the introduced Chinese populations.Our study provides novel phenotypic and genomic evidence showing how smooth cordgrass rapidly adapts to variable environmental conditions in its introduced ranges.Moreover,candidate genes related toflowering time,fast growth,and stress tolerance(i.e.,salinity and submergence)provide valuable genetic resources for future improvement of cereal crops.
基金The key research program of State Forestry Administration of China(2006-39)
文摘对原产湖北五峰的红花玉兰新变种多瓣红花玉兰(Magnolia wufengensisvar.multitepalaL.Y.Ma et L.R.W ang)作了描述。新变种与红花玉兰原变种(M.wufengensisvar.wufengensis)花色相近,整个花被片内外均为红色,内面略淡。主要区别在于:新变种花被片12、15、18、24;花被片阔倒卵状匙形、倒卵状匙形或窄倒卵状匙形;花色浓淡不同植株间有深红、红、浅红之差别;自然状态下可育,蓇葖果发育良好。