Nasopharyngeal carcinoma(NPC)is the third most common malignancy with a high recurrence and metastasis rate in South China.Natural compounds extracted from traditional Chinese herbal medicines have been developed and ...Nasopharyngeal carcinoma(NPC)is the third most common malignancy with a high recurrence and metastasis rate in South China.Natural compounds extracted from traditional Chinese herbal medicines have been developed and utilized for the treatment of a variety of cancers with modest properties and slight side effects.Maackiain(MA)is a type of flavonoid that was first isolated from leguminous plants,and it has been reported to relieve various nervous system disorders and exert anti-allergic as well as antiinflammatory effects.In this study,we demonstrated that MA inhibited proliferation,arrested cell cycle and induced apoptosis in nasopharyngeal carcinoma CNE1 and CNE2 cells in vitro and in vivo.The expression of the related proteins associated with these processes were consistent with the above effects.Moreover,transcriptome sequencing and subsequent Western blot experiments revealed that inhibition of the MAPK/Ras pathway may be responsible to the anti-tumor effect of MA on NPC cells.Therefore,the effects of MA and an activator of this pathway,tertiary butylhydroquinone(TBHQ),alone or combination,were investigated.The results showed TBHQ neutralized the inhibitory effects of MA.These data suggest that MA exerts its anti-tumor effect by inhibiting the MAPK/Ras signaling pathway and it has the potential to become a treatment for patients with NPC.展开更多
[Objectives] This study aimed to investigate the quality of Radix Millettiae Speciosae at different planting sites in Guangxi. [Methods] The content of flavonoids in Radix Millettiae Speciosae was determined by HPLC. ...[Objectives] This study aimed to investigate the quality of Radix Millettiae Speciosae at different planting sites in Guangxi. [Methods] The content of flavonoids in Radix Millettiae Speciosae was determined by HPLC. The chromatographic conditions were as follows: column, ZORBAX SB-C_(18); mobile phase, acetonitrile-0.1% glacial acetic acid(42∶58); detection wavelength, 250(formononetin) and 310(maackiain) nm; flow rate, 1.0 mL/min; and column temperature, 30℃. [Results] Under the chromatographic conditions above, formononetin and maackiain could be completely separated from impurities. The standard curve had a good linear relationship(R^2>0.999 9). The precision and stability met analysis requirements. The average recovery rates were 97.53% and 98.54%, respectively, and the RSD values were 2.03% and 1.88%, respectively, indicating that the established method has good reproducibility, durability and accuracy. The content of formononetin was highest in the Radix Millettiae Speciosae from Yongning District, Nanning City, and the content of maackiain was highest in the Radix Millettiae Speciosae from Xichang Town, Hepu County. [Conclusions] The HPLC method established in this study is simple, accurate and stable. It can be used as a quality control method for Radix Millettiae Speciosae. The contents of flavonoids in Radix Millettiae Speciosae from Yongning District, Nanning City and Xichang Town, Hepu County were higher than those from other planting bases.展开更多
基金the Independent Subject of Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement(No.KL2022ZZ01)Guangxi Innovation-Driven Development Project(No.GuiKe AA18242040)+3 种基金the National Key R&D Program of China(Nos.2019YFC1711000 and 2019YFC1711008)the Innovation Team Project of Guangxi Botanical Garden of Medicinal Plant(No.GuiYaoChuang2019002)the Innovation and Demonstration of Guangxi Academician Workstation(No.2021AV07007)Government Guided Local Science and Technology Development Project(No.GuiKeZY 20198018).
文摘Nasopharyngeal carcinoma(NPC)is the third most common malignancy with a high recurrence and metastasis rate in South China.Natural compounds extracted from traditional Chinese herbal medicines have been developed and utilized for the treatment of a variety of cancers with modest properties and slight side effects.Maackiain(MA)is a type of flavonoid that was first isolated from leguminous plants,and it has been reported to relieve various nervous system disorders and exert anti-allergic as well as antiinflammatory effects.In this study,we demonstrated that MA inhibited proliferation,arrested cell cycle and induced apoptosis in nasopharyngeal carcinoma CNE1 and CNE2 cells in vitro and in vivo.The expression of the related proteins associated with these processes were consistent with the above effects.Moreover,transcriptome sequencing and subsequent Western blot experiments revealed that inhibition of the MAPK/Ras pathway may be responsible to the anti-tumor effect of MA on NPC cells.Therefore,the effects of MA and an activator of this pathway,tertiary butylhydroquinone(TBHQ),alone or combination,were investigated.The results showed TBHQ neutralized the inhibitory effects of MA.These data suggest that MA exerts its anti-tumor effect by inhibiting the MAPK/Ras signaling pathway and it has the potential to become a treatment for patients with NPC.
基金Supported by Special Project of Guangxi Education Department for the Construction of Advantageous Subjects of Traditional Chinese Medicine(ZYX2015006)
文摘[Objectives] This study aimed to investigate the quality of Radix Millettiae Speciosae at different planting sites in Guangxi. [Methods] The content of flavonoids in Radix Millettiae Speciosae was determined by HPLC. The chromatographic conditions were as follows: column, ZORBAX SB-C_(18); mobile phase, acetonitrile-0.1% glacial acetic acid(42∶58); detection wavelength, 250(formononetin) and 310(maackiain) nm; flow rate, 1.0 mL/min; and column temperature, 30℃. [Results] Under the chromatographic conditions above, formononetin and maackiain could be completely separated from impurities. The standard curve had a good linear relationship(R^2>0.999 9). The precision and stability met analysis requirements. The average recovery rates were 97.53% and 98.54%, respectively, and the RSD values were 2.03% and 1.88%, respectively, indicating that the established method has good reproducibility, durability and accuracy. The content of formononetin was highest in the Radix Millettiae Speciosae from Yongning District, Nanning City, and the content of maackiain was highest in the Radix Millettiae Speciosae from Xichang Town, Hepu County. [Conclusions] The HPLC method established in this study is simple, accurate and stable. It can be used as a quality control method for Radix Millettiae Speciosae. The contents of flavonoids in Radix Millettiae Speciosae from Yongning District, Nanning City and Xichang Town, Hepu County were higher than those from other planting bases.
