The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'd...The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'dialogues from the perspective of politeness principle,one conception of which is that politeness is a social behavior in the contact of human beings,the strategies of Mabel's love pursuing and acquiring are gradually revealed.展开更多
Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herei...Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in tomatoes.Conversely, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.展开更多
单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。...单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。使用NASA(National Aeronautics and Space Administration)的机载激光雷达MABEL(Multiple Altimeter Beam Experimental LiDAR)的实际飞行数据进行了实验,并通过模拟仿真的方法对实验结果做了验证。结果表明,此方法能有效地剔除噪声,试验区的整体精度能达到97.8%。展开更多
文摘The Horse Dealer's Daughter gradually acquires her love in a tough and unbearable situation after a long dialogue with Doctor Fergusson.By analyzing the linguistic features between Doctor Fergusson and Mabel'dialogues from the perspective of politeness principle,one conception of which is that politeness is a social behavior in the contact of human beings,the strategies of Mabel's love pursuing and acquiring are gradually revealed.
基金supported by the National Natural Science Foundation of China (grant no. 32202554)the earmarked fund for CARS (grant no. CARS-31)+3 种基金Guangdong Banana and Pineapple Industry Technology System Innovation Team(grant no. 2022KJ109)Natural Science Foundation of Guangdong Province (2023A1515010335)Pearl River Talent Program for Young Talent (grant no. 2017GC010321)Young Innovative Talents Projects in Ordinary Colleges and Universities in Guangdong Province (grant no.2021KQNCX005)。
文摘Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder.However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in tomatoes.Conversely, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.
文摘单光子激光测高已然成为激光测高领域的发展趋势。面对高重频、剖面式单光子激光数据,在参考国内外激光点云数据处理算法和对单光子激光数据特点精确分析的基础上,提出了一种基于地形相关和最小二乘曲线拟合的单光子激光数据处理方法。使用NASA(National Aeronautics and Space Administration)的机载激光雷达MABEL(Multiple Altimeter Beam Experimental LiDAR)的实际飞行数据进行了实验,并通过模拟仿真的方法对实验结果做了验证。结果表明,此方法能有效地剔除噪声,试验区的整体精度能达到97.8%。
