A novel branched block copolymer was synthesized with self-condensing vinyl polymerization(SCVP)of macroinimer,PEGMA-b-PCL-Br,via atom transfer radical polymerization(ATRP).The macro-inimer was prepared with enzyme-ca...A novel branched block copolymer was synthesized with self-condensing vinyl polymerization(SCVP)of macroinimer,PEGMA-b-PCL-Br,via atom transfer radical polymerization(ATRP).The macro-inimer was prepared with enzyme-catalyzed ring-opening polymerization(ROP)of ε-caprolactone(ε-CL)with poly(ethylene glycol)methacrylate(PEGMA)as the initiator and esterification of the ω-hydroxyl group of the obtained PEGMA-PCL with α-bromoisobutyryl bromide.展开更多
This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compou...This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model.展开更多
文摘A novel branched block copolymer was synthesized with self-condensing vinyl polymerization(SCVP)of macroinimer,PEGMA-b-PCL-Br,via atom transfer radical polymerization(ATRP).The macro-inimer was prepared with enzyme-catalyzed ring-opening polymerization(ROP)of ε-caprolactone(ε-CL)with poly(ethylene glycol)methacrylate(PEGMA)as the initiator and esterification of the ω-hydroxyl group of the obtained PEGMA-PCL with α-bromoisobutyryl bromide.
基金supported by the Province Natural Science Foundation of Shandong (Grant number 2009ZRB02230)
文摘This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model.
