AIM:To study the methods of preparing the magnetic nano-microspheres of Fe2O3 and As2O3/Fe2O3 complexes and their therapeutic effects with magnetic fluid hyperthermia(MFH). METHODS:Nanospheres were prepared by chemica...AIM:To study the methods of preparing the magnetic nano-microspheres of Fe2O3 and As2O3/Fe2O3 complexes and their therapeutic effects with magnetic fluid hyperthermia(MFH). METHODS:Nanospheres were prepared by chemical co-precipitation and their shape and diameter were observed.Hemolysis,micronucleus,cell viability,and LD50 along with other in vivo tests were performed to evaluate the Fe2O3 microsphere biocompatibility.The inhibition ratio of tumors after Fe2O3 and As2O3/Fe2O3 injections combined with induced hyperthermia in xenograft human hepatocarcinoma was calculated. RESULTS:Fe2O3 and As2O3/Fe2O3 particles were round with an average diameter of 20 nm and 100 nm as observed under transmission electron microscope.Upon exposure to an alternating magnetic field(AMF),the temperature of the suspension of magnetic particles increased to 41-51℃,depending on different particle concentrations,and remained stable thereafter.Nanosized Fe2O3 microspheres are a new kind of biomaterial without cytotoxic effects.The LD50 of both Fe2O3 and As2O3/Fe2O3 in mice was higher than 5 g/kg.One to four weeks after Fe2O3 and As2O3/Fe2O3 complex injections into healthy pig livers,no significant differences were found in serum AST,ALT,BUN and Cr levels among thepigs of all groups(P>0.05),and no obvious pathological alterations were observed.After exposure to alternating magnetic fields,the inhibition ratio of the tumors was significantly different from controls in the Fe2O3 and As2O3/Fe2O3 groups(68.74% and 82.79%,respectively; P<0.01).Tumors of mice in treatment groups showed obvious necrosis,while normal tissues adjoining the tumor and internal organs did not. CONCLUSION:Fe2O3 and As2O3/Fe2O3 complexes exerted radiofrequency-induced hyperthermia and drug toxicity on tumors without any liver or kidney damage. Therefore,nanospheres are ideal carriers for tumortargeted therapy.展开更多
CoFe2O4 nanoparticles(NPs)and surface modified with gold(Au)have been synthesized by a thermal decomposition method.The obtained NPs and formation of CoFe2O4@Au core–shell(CS)were confirmed by characterizing their st...CoFe2O4 nanoparticles(NPs)and surface modified with gold(Au)have been synthesized by a thermal decomposition method.The obtained NPs and formation of CoFe2O4@Au core–shell(CS)were confirmed by characterizing their structural and optical properties using X-ray powder diffraction(XRD)patterns,Fourier transform infrared spectroscopy,Raman spectroscopy,UV–Visible and photoluminescence studies.Morphological and compositional studies were carried out using high-resolution transmission electron microscopy and energy-dispersive X-ray spectroscopy,while the magnetic properties were determined using alternating gradient magnetometer and Mossbauer to define the magneto-structural effects of shell formation on the core NPs.Induction heating properties of CoFe2O4 and CoFe2O4@Au CS magnetic nanoparticles(MNPs)have been investigated and correlated with magneto-structural properties.Specific absorption rate and intrinsic loss power were calculated for these MNPs within the human tolerable range of frequency and amplitude,suggesting their potential in magnetic fluid hyperthermia therapy for possible cancer treatment.展开更多
基金Supported by The National Natural Science Foundation of China,30770584the State 863 Plan,2002AA302207,2007AA03Z356
文摘AIM:To study the methods of preparing the magnetic nano-microspheres of Fe2O3 and As2O3/Fe2O3 complexes and their therapeutic effects with magnetic fluid hyperthermia(MFH). METHODS:Nanospheres were prepared by chemical co-precipitation and their shape and diameter were observed.Hemolysis,micronucleus,cell viability,and LD50 along with other in vivo tests were performed to evaluate the Fe2O3 microsphere biocompatibility.The inhibition ratio of tumors after Fe2O3 and As2O3/Fe2O3 injections combined with induced hyperthermia in xenograft human hepatocarcinoma was calculated. RESULTS:Fe2O3 and As2O3/Fe2O3 particles were round with an average diameter of 20 nm and 100 nm as observed under transmission electron microscope.Upon exposure to an alternating magnetic field(AMF),the temperature of the suspension of magnetic particles increased to 41-51℃,depending on different particle concentrations,and remained stable thereafter.Nanosized Fe2O3 microspheres are a new kind of biomaterial without cytotoxic effects.The LD50 of both Fe2O3 and As2O3/Fe2O3 in mice was higher than 5 g/kg.One to four weeks after Fe2O3 and As2O3/Fe2O3 complex injections into healthy pig livers,no significant differences were found in serum AST,ALT,BUN and Cr levels among thepigs of all groups(P>0.05),and no obvious pathological alterations were observed.After exposure to alternating magnetic fields,the inhibition ratio of the tumors was significantly different from controls in the Fe2O3 and As2O3/Fe2O3 groups(68.74% and 82.79%,respectively; P<0.01).Tumors of mice in treatment groups showed obvious necrosis,while normal tissues adjoining the tumor and internal organs did not. CONCLUSION:Fe2O3 and As2O3/Fe2O3 complexes exerted radiofrequency-induced hyperthermia and drug toxicity on tumors without any liver or kidney damage. Therefore,nanospheres are ideal carriers for tumortargeted therapy.
基金Author (Sandip Sabale) is thankful to University Grants Commission, New Delhi, India, for Raman Fellowship to work in USA (F. No. 5-105/2016 (IC), February 10, 2016
文摘CoFe2O4 nanoparticles(NPs)and surface modified with gold(Au)have been synthesized by a thermal decomposition method.The obtained NPs and formation of CoFe2O4@Au core–shell(CS)were confirmed by characterizing their structural and optical properties using X-ray powder diffraction(XRD)patterns,Fourier transform infrared spectroscopy,Raman spectroscopy,UV–Visible and photoluminescence studies.Morphological and compositional studies were carried out using high-resolution transmission electron microscopy and energy-dispersive X-ray spectroscopy,while the magnetic properties were determined using alternating gradient magnetometer and Mossbauer to define the magneto-structural effects of shell formation on the core NPs.Induction heating properties of CoFe2O4 and CoFe2O4@Au CS magnetic nanoparticles(MNPs)have been investigated and correlated with magneto-structural properties.Specific absorption rate and intrinsic loss power were calculated for these MNPs within the human tolerable range of frequency and amplitude,suggesting their potential in magnetic fluid hyperthermia therapy for possible cancer treatment.
