Magnoline（1）,a new isoquinoline derivative,was isolated from the leaves of Magnolia sieboldii K.Koch（Magnoliaceae）.Its structure was elucidated on the basis of spectral analysis including 1D,2D NMR and HR-TOF-MS.
Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extrac...Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR(quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level.展开更多
收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁...收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁育手段、种子休眠解除机制、药用价值、开花生物学特性和抗寒机制.其中,种子变温层积催芽技术是天女木兰种子繁殖研究的主要内容,而无性繁殖技术的研究则侧重于筛选能够获得最佳繁殖效果的处理方法;关键基因鉴定是种子休眠解除机制的探索重点;精油成分功效以及木香烃内酯导致细胞凋亡的途径是天女木兰药用价值的研究热点;通过了解天女木兰开花生物学特性研究濒危树种保育对策;抗寒机制的研究集中于抗逆生理响应.制订科学的繁殖技术方案,加强生态适应机制和适应能力研究,保护与开发有机结合应成为今后天女木兰研究的主攻方向.展开更多
文摘Magnoline（1）,a new isoquinoline derivative,was isolated from the leaves of Magnolia sieboldii K.Koch（Magnoliaceae）.Its structure was elucidated on the basis of spectral analysis including 1D,2D NMR and HR-TOF-MS.
基金supported by the National Natural Science Foundation of China(No.31570621)
文摘Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA(ribonucleic acid)extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR(quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level.
文摘收集Web of Science^(TM)和CNKI文献检索数据库中的天女木兰资料,利用Citespace可视化文献计量工具分析1980—2023年天女木兰研究领域的202篇有效文献,根据关键词共现和关键词聚类时间轴知识图谱,总结当前天女木兰的热门研究话题——繁育手段、种子休眠解除机制、药用价值、开花生物学特性和抗寒机制.其中,种子变温层积催芽技术是天女木兰种子繁殖研究的主要内容,而无性繁殖技术的研究则侧重于筛选能够获得最佳繁殖效果的处理方法;关键基因鉴定是种子休眠解除机制的探索重点;精油成分功效以及木香烃内酯导致细胞凋亡的途径是天女木兰药用价值的研究热点;通过了解天女木兰开花生物学特性研究濒危树种保育对策;抗寒机制的研究集中于抗逆生理响应.制订科学的繁殖技术方案,加强生态适应机制和适应能力研究,保护与开发有机结合应成为今后天女木兰研究的主攻方向.
