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Genetic diversity and gene structure of mitochondrial region of Anopheles minimus(Diptcra:Culicidae)-major malaria vector of North east India
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作者 Prafulla Dutta Siraj Ahmed Khan +4 位作者 Rashmee Topno Pritom Chowdhury Mayuri Baishya Purvita Chowdhury Jagadish Mahanta 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2014年第12期952-955,共4页
Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(An.minimus)(Diptera:Culicidae) species from two malaria endemic states of NE India.Methods:Phylogeographic analysis was ca... Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(An.minimus)(Diptera:Culicidae) species from two malaria endemic states of NE India.Methods:Phylogeographic analysis was carried at 9 out of 12 sites oi An.minimus confirmed malaria endemic places.Results:All sequences were Adenine-Thymine rich regions.Transitions were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 ease non synonymous mutation was observed.Three distinct clusters of haplolypes were generated.Haplotvpe diversity and low nucleotide diversity were studied.Overall negative values obtained from Tajima's D test and Fu'sF_S test indicate a recent genetic population expansion.Network analysis has explained sequence diversity that was also shown by mutations in 6 sequences.Conclusions:High genetic diversity observed within the populations of An.minimus species has several possible implications for vector control in the region. 展开更多
关键词 anopheles minimus CYTOCHROME OXIDASE HAPLOTYPE Mutation Substitution
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云南微小按蚊(Anophelesminimus)种型和对间日疟原虫感染性的初步研究 被引量:10
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作者 王学忠 王丕玉 李菊升 《寄生虫与医学昆虫学报》 CAS 1995年第4期223-227,共5页
本文对云南不同地理株微小按蚊染色体核型及对间日疟原虫敏感性作了比较研究。经对性染色体相对长度和着丝点,G带染色体比较结果,发现Y染色体上呈现出三种不同的着丝点,对间日疟原虫敏感性上也存在一定的差异。
关键词 微小按蚊 种型 疟原虫 间日疟原虫 感染性
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云南元江流域疟疾防治后微小按蚊(Anopheles minimus Theobald)生物学特性和相关生态学因子研究 被引量:5
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作者 许建卫 杨煌 +9 位作者 顾云安 马信文 杨学文 刘行知 汪丽波 杨宗强 杨继青 杨太专 苏蓉 杨国灿 《医学动物防制》 1999年第5期225-228,共4页
在云南元江流域,通过5年的面上监测和1年的设点研究,发现杀虫剂使用后40多年,微小按蚊的活动从室内转到室外,从村子内转到野外,人类和微小按蚊间的疟原虫传递多发生在室外;影响河谷区微小按蚊种群数量的关键生态学因子是降水;温度是影... 在云南元江流域,通过5年的面上监测和1年的设点研究,发现杀虫剂使用后40多年,微小按蚊的活动从室内转到室外,从村子内转到野外,人类和微小按蚊间的疟原虫传递多发生在室外;影响河谷区微小按蚊种群数量的关键生态学因子是降水;温度是影响其寿命的首要因子,其次是相对湿度。上述因素影响微小按蚊密度和叮人率;7—8月份种群数量不大,但病例却较多。结果揭示使用了40多年的DDT室内滞留喷洒为主的疟疾媒介控制措施不能完全有效地阻断疟疾传播。 展开更多
关键词 疟疾 微小按蚊 生物学特性 生态因子 元江流域
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滇西南地区微小按蚊(Anopheles minimus)实验室驯化种群的建立
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作者 王丕玉 王学忠 杜尊伟 《医学动物防制》 1999年第2期62-64,共3页
为开展对云南微小按蚊遗传多态性与传疟作用的研究需建立实验室品系。将羽化后4d成蚊进行自然交配试验,结果550只雌蚊产卵1192枚,平均2.17枚;孵化、化蛹、羽化率分别为63.51%、62.48%、87.53%,获第1代自然交配繁殖成蚊414只。然后采... 为开展对云南微小按蚊遗传多态性与传疟作用的研究需建立实验室品系。将羽化后4d成蚊进行自然交配试验,结果550只雌蚊产卵1192枚,平均2.17枚;孵化、化蛹、羽化率分别为63.51%、62.48%、87.53%,获第1代自然交配繁殖成蚊414只。然后采用人工交配与自然交配繁殖同龄成蚊混合驯化观察3代,产卵从最初的平均2.17枚提高到9.22枚;孵化率也从63.51%上升到92.67%。实验表明此法是一种简便、快速、有效的驯化方法。 展开更多
关键词 微小按蚊 实验室饲养 驯化
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Larvicidal and repellent potential of Moringa oleifera against malarial vector,Anopheles stephensi Liston(Insecta:Diptera:Culicidae) 被引量:19
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作者 Prabhu K Murugan K +2 位作者 Nareshkumar A Ramasubramanian N Bragadeeswaran S 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2011年第2期124-129,共6页
Objective:To evaluate the larvicidal and pupicidal potential of the methanolic extracts from Moringa oleifera(M.oleifera) plant seeds against malarial vector Anopheles stephensi(A.stephensi) mosquitoes at different co... Objective:To evaluate the larvicidal and pupicidal potential of the methanolic extracts from Moringa oleifera(M.oleifera) plant seeds against malarial vector Anopheles stephensi(A.stephensi) mosquitoes at different concentrations(20,40,60,80 and 100 ppm).Methods:M.oleifera was collected from the area of around Bharathiar University,Coimbatore.The dried plant materials were powdered by an electrical blender.From each sample,100 g of the plant material were extracted with 300 mL of methanol for 8 h in a Soxhlet apparatus.The extracts were evaporated to dryness in rotary vacuum evaporator to yield 122 mg and 110 mg of dark greenish material(residue) from Arcang amara and Ocimum basilicum,respectively.One gram of the each plant residue was dissolved separately in 100 mL of acetone(stock solution) from which different concentrations, i.e.,20,40,60,80 and 100 ppm were prepared.Results:Larvicidal activity of M.oleifera exhibited in the first to fourth instar larvae of the A.stephensi,and the LC_(50) and LC_(90) values were 57.79 ppm and 125.93 ppm for the first instar,63.90 ppm and 133.07 ppm for the second inslar,72.45 ppm and 139.82 ppm for the third instar,78.93 ppm and 143.20 ppm for the fourth instar,respectively. During the pupal stage the methanolic extract of M.oleifera showed that the LC_(50),and LC_(90) values were 67.77 ppm and 141.00 ppm,respectively.Conclusions:The present study indicates that the phytochemicals derived from M.oleifera seeds extracts are effective mosquito vector control agents and the plant extracts may be used for further integrated pest management programs. 展开更多
关键词 Moringa oleifera anopheles STEPHENSI Insecticide LARVICIDE Pupicide malaria Phytochemical Mosquito VECTOR control Plant extract LARVICIDAL activity
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Toxicity of several botanical essential oils and their combinations against females of Aedes albopictus(Skuse) and Anopheles minimus(Theobald): Oviposition deterrent, ovicidal and adulticidal efficacies 被引量:3
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作者 Nuttavich Cotchakaew Mayura Soonwera 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2019年第1期29-39,共11页
Objective: To investigate the efficacies of 12 essential oil(EO) formulations from three Zingiberaceae plants(Alpinia galanga, Curcuma zedoaria, and Zingiber cassumunar) individually and in combination with an augment... Objective: To investigate the efficacies of 12 essential oil(EO) formulations from three Zingiberaceae plants(Alpinia galanga, Curcuma zedoaria, and Zingiber cassumunar) individually and in combination with an augmenting Eucalyptus globulus(E. globulus) EO against females of Aedes albopictus(Ae. albopictus) and Anopheles minimus(An. minimus). Methods: These formulations were evaluated for their ovicidal, oviposition deterrent and adulticidal activities against Ae. albopictus and An. minimus by a topical method, a double-choice method and a WHO susceptibility test, respectively. Results: It was found that all formulations of Zingiberaceae plants EOs augmented with E. globulus EO were more effective in oviposition deterrent, ovicidal, and adulticidal activities against the two mosquito species than all of the formulations used without E. globulus EO. Their oviposition deterrent, ovicidal and adulticidal activities were equivalent to those of 10% w/v cypermethrin. In contrast, 70% v/v ethyl alcohol as a control alone was not effective at all. The highest synergistic effect in effective repellency against Ae. albopictus was achieved by 5% Alpinia galanga EO + 5% E. globulus EO and against An. minimus was 5% Zingiber cassumunar EO + 5% E. globulus EO. Moreover, the highest synergistic effects in ovicidal activities against Ae. albopictus and An. minimus were achieved by 10% Zingiber cassumunar EO + 10% E. globulus EO and 5% Curcuma zedoaria EO + 5% E. globulus EO, respectively. For the adulticidal activities, the highest synergistic effect against two mosquitoes was achieved by 5% Curcuma zedoaria EO + 5% E. globulus EO. Conclusions: These results suggest that Zingiberaceae plant EOs augmented with E. globulus EO have a high potential to be developed into oviposition deterrent, ovicidal, and adulticidal agents for controlling populations of Ae. albopictus and An. minimus. 展开更多
关键词 AEDES ALBOPICTUS anopheles minimus Plant essential oil OVICIDE OVIPOSITION DETERRENT Adulticide
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Some ecological attributes of malarial vector Anopheles superpictus Grassi in endemic foci in southeastern Iran 被引量:2
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作者 Jalil Nejati Hasan Vatandoost +3 位作者 Mohammad Ali Oshghi Masud Salehi Ehssan Mozafari Seyed Hasan Moosa-Kazemi 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第12期1003-1008,共6页
Objective:To determine the bionomics and susceptibility status of the malarial vector Anopheles superpictus(An.superpictus)to different insecticides in the Sistan-Baluchestan province which has the highest malarial pr... Objective:To determine the bionomics and susceptibility status of the malarial vector Anopheles superpictus(An.superpictus)to different insecticides in the Sistan-Baluchestan province which has the highest malarial prevalence in Iran.Methods:Different sampling methods,in addition to scoring abdominal conditions,were used to define the seasonal activity and endo/exophilic behavior of this species.In addition,the standard WHO susceptibility tests were applied on adult field strains.Results:Most adult mosquitoes were collected from outdoor shelters.The peak of seasonal activity of An.superpictus occurred at the end of autumn.Most larvae were collected from natural and permanent breeding places with full sunlight and no vegetation.Blood feeding activities occurred around midnight.Compared with the abdominal conditions of adult mosquitoes collected indoors,the abdominal conditions of adult mosquitoes collected outdoors were gravid and seniigravid.This species was suspected to be resistant to DDT,but was susceptible to other insecticides.Conclusions:An.superpictus was present in almost all outdoor shelters,and the ratios of gravid,semigravid/unfed,and freshly fed confirmed that this species had a higher tendency to rest outdoors than indoors.This behavior can protect An.superpictus from indoor residual spraying in this malarious area.To the best of our knowledge,this is the first report on the susceptibility status of An.superpictus in Southeastern Iran.We do not suggest the use of DDT for indoor residual spraying in southeast Iran. 展开更多
关键词 anopheles superpictus malaria Iran
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Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors 被引量:2
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作者 Damrongpan Thongwat Ratchanaporn Chokchaisiri +1 位作者 Lucksagoon Ganranoo Nophawan Bunchu 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2018年第5期273-278,共6页
Objective:To evaluate the larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri endocarp against Aedes aegypti,Aedes albopictus,Culex quinquefasciatus,and Anopheles minimus mosquitos.Methods:Larv... Objective:To evaluate the larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri endocarp against Aedes aegypti,Aedes albopictus,Culex quinquefasciatus,and Anopheles minimus mosquitos.Methods:Larvicidal activity was tested according to World Health Organization standard protocol.The third-stage larvae of each mosquito species were exposed to various concentrations of Dracaena loureiri crude extract and six groups of Dracaena loureiri fractionated extracts(RC-DT 009-014).Larval mortality rates were observed after 24 h and48 h of exposure.Then,a computerized probit analysis of the mortality data was performed to determine lethal concentration 50(LC_(50))and lethal concentration 90 values.Results:Anopheles minimus larvae(24-h LC_(50)77.88 mg/L)had the highest susceptibility to crude extract,whereas others(Aedes aegypti,24-h LC_(50)224.73 mg/L;Aedes albopictus,24-h LC_(50)261.75 mg/L;and Culex quinquefasciatus,24-h LC_(50)282.86 mg/L)were significantly less susceptible.The most effective groups of fractionated extracts were RC-DT 012 and RC-DT 013.The mosquito species most susceptible to fractionated extracts was Culex quinquefasciatus,with 24-h LC_(50)values of 0.66 and 0.94 mg/L for RC-DT 012 and RC-DT 013,respectively.Conclusions:The larvicidal activity of fractionated extracts is more effective than that of crude extract against all tested mosquito species.For the most effective alternative larvicide,purification and a phytochemical constituent analysis must be performed. 展开更多
关键词 Dracaena loureiri Aedes aegypti Aedes albopictus Culex quinquefasciatus anopheles minimus Fractionated extract Crude extract LARVICIDAL Mosquito larva
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Indication of pyrethroid resistance in the main malaria vector Anopheles stephensi from Iran 被引量:2
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作者 Hassan Vatandoost Ahmad Ali Hanafi-Bojd 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2012年第9期722-726,共5页
ObjectiveTo investiagte insecticide resistance in target species for better insecticide resistance managemnet in malaria control programs.MethodsThe status of insecticide resistance to different imagicides in Anophele... ObjectiveTo investiagte insecticide resistance in target species for better insecticide resistance managemnet in malaria control programs.MethodsThe status of insecticide resistance to different imagicides in Anopheles stephensi (An. stephensi) including DDT 4%, lambdacyhalothrin 0.50%, deltamethrin 0.05%, permethrin 0.75%, cyfluthrin 0.15% and etofenprox 0.50% was performed according to WHO standard method.ResultsThe mortality rate to lambdacyhalothrin, permethrin, cyfluthrin, deltamethrin, etofenprox and DDT was (88.0±3.2), (92.0±2.7), (52.0±5.0), (96.0±2.2), (90.0±3.0) and (41.0±5.7) percent, respectively at diagnostic dose for one hour exposure time followed by 24 h recovery period.ConclusionsThese results showed first indication of pyrethroid resistance in An. stephensi in a malarious area, from southern Iran. There is widespread, multiple resistances in the country in An. stephensi to organochlorine and some report of tolerance to organophosphate insecticides and recently to pyrethroids. However, results of this paper will provide a clue for monitoring and mapping of insecticide resistance in the main malaria vector for implementation of any vector control. 展开更多
关键词 Anophels stephensi malaria PESTICIDES Resisatnce
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Irritability of malaria vector,Anopheles sacharovi to different insecticides in a malaria-prone area 被引量:1
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作者 Hassan Vatandoost Mohammad Reza Abai 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2012年第2期113-116,共4页
Objective:To determine the susceptibility and irritability level of malaria vector Anopheles sacharovi(An.sachrovi) to different insecticides in a malaria-prone area.Methods:Susceptibility and irritability levels of f... Objective:To determine the susceptibility and irritability level of malaria vector Anopheles sacharovi(An.sachrovi) to different insecticides in a malaria-prone area.Methods:Susceptibility and irritability levels of field collected strain of An.sacharovi to WHO standard papers of DDT 4%,dieldrin 0.4%,malathion 5%,fenitrothion 1%,permethrin 0.75%,and deltamethrin 0.05% were determined in East Azerbaijan of Iran during reemerging of malaria as described by WHO.Results:Results showed that at the diagnostic dose of insecticides this species exhibited resistance to DDT,tolerant to dieldrin and but somehow susceptible to fenitrothion,malathion, permethrin and deltamethrin.The results of irritability of this species to DDT,lambdacyhalothrin. permethrin cyfluthrin and deltamethrin revealed that DDT had had the most and deltamethrin the least irritancy effect.The average number of take offs/fly/minules for DDT was 0.8±0.2.The order of irritability for permethrin,lambdacyhalothrin,cyfluthrin and deltamethrin were 0.7±0.2,0.5±0.2, 0.5±0.3,and 0.2±0.1,respectively.Conclusions:Results of this study reveals the responsiveness of the main malaria vector to different insecticides.This phenomenon is depending on several factors such as type and background of insecticide used previously,insecticide properties,and physiology of the species.Careful monitoring of insecticide resistance and irritability level of species could provide a clue for appropriate selection of insecticide for malaria control. 展开更多
关键词 anopheles sacharovi INSECTICIDES malaria
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Malaria Outbreaks in Villages in North Karnataka, India, and Role of Sibling Species of <i>Anopheles culicifacies</i>Complex
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作者 Satyanarayan Tiwari Susanta Kumar Ghosh +3 位作者 Thiruchrapalli S. Sathyanarayan Nutan Nanda Sreehari Uragayala Neena Valecha 《Health》 2015年第8期946-954,共9页
Investigations on malaria outbreaks and role of sibling species complex of principal rural malaria vector Anopheles culicifacies were carried out in villages in north Karnataka, India from 1997 through 2014. Informati... Investigations on malaria outbreaks and role of sibling species complex of principal rural malaria vector Anopheles culicifacies were carried out in villages in north Karnataka, India from 1997 through 2014. Information regarding densities, resting and breeding habitats of malaria vectors prevalent in the area was also generated so as to formulate an appropriate vector control strategy. The Slide Positivity Rate (SPR), Slide Falciparum Rate (SFR) and Pf proportion was 43.1%, 35.9% and 83.3%, respectively. Three sibling species A, B, and C of An. culicifacies were found sympatric with cumulative percent composition of 63.7, 28.2 and 8.1, respectively. Per man hour and per structure densities of An. culicifacies, An. culicifacies and An. culicifacies varied from 0 to 27.5 and 0 to 56.0, 0 to 0.5 and 0 to 7.0 and, 0 to 2.5 and 0 to 7.5, respectively. The proportion of semi-gravid and gravid females was more as compared to fully fed and unfed females which indicated that most of the females rested indoor. Streams/river, wells, seepages and irrigation tanks are the major habitats supporting breeding of An. culicifacies. Integrated vector managements by indoor residual spraying of effective insecticide as per national guidelines along with biological control methods especially use of larvivorous fish Gambusia affinis and Poecilia reticulate are suggested to control malaria in the area. 展开更多
关键词 malaria anopheles culicifacies SIBLING Species Biological Control NORTH KARNATAKA
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Astrodaucus persicus as a new source of bioinsectisides against malaria vector, Anopheles stephensi
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作者 Saied Goodarzi Hassan Vatandoost +6 位作者 Mohammad Reza Abai Saeed Tavakoli Amir Hatamian Yousef Ajani Abbas Hadjiakhoondi Narguess Yassa Zahra Tofighi 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2017年第9期896-899,共4页
Objective: To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, ... Objective: To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, Anopheles stephensi(An. stephensi).Methods: Twenty five third instar larvae of An. stephensi were exposed to various concentrations(10–160 g/L) of fractions and were assayed according to World Health Organization protocol. The larval mortality was calculated after 24 h treatment.Results: Among tested fractions, the highest larvicidal efficacy was observed from ethyl acetate fraction of fruits extract with 50% and 90% mortality values(LC_(50) and LC_(90)) of34.49 g/L and 108.61 g/L, respectively. Chloroform fraction of fruits extract was the second larvicidal sample with LC_(50) of 45.11 g/L and LC_(90) of 139.36 g/L. Petroleum ether fractions of fruits and roots and methanol fraction of fruits showed moderate toxicity against An. stephensi.Conclusions: Astrodaucus persicus is a potential source of valuable and natural larvicidal compounds against malaria vector, An. stephensi and can be used in mosquitoes control programs as an alternative to synthetic insecticides. 展开更多
关键词 Astrodaucus persicus malaria vector Mosquitoes control BIOINSECTICIDE anopheles stephensi larvae
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Investigation of the Role of ABC Transporters in Pyrethroids Resistance in the Major Malaria Vector Anopheles coluzzii from Northern Nigeria
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作者 Zilkifil Abulhassan Binta G. Kurfi +2 位作者 Sulaiman Sadi Ibrahim Daha Umar Ishaq Muhammad Mahe Mukthar 《Open Journal of Immunology》 2019年第3期29-35,共7页
Resistance to currently available insecticides in the major malaria vectors like Anopheles coluzzii is seriously reducing the effectiveness of core vector control tools. Overexpression of ATP binding cassette transpor... Resistance to currently available insecticides in the major malaria vectors like Anopheles coluzzii is seriously reducing the effectiveness of core vector control tools. Overexpression of ATP binding cassette transporters (ABC transporters) has been implicated in insecticides resistance in the major malaria vector Anopheles funestus and An. gambiae. To identify the potential role of ABC transporters in pyrethroids resistance in this work, we use verapamil-a p-glycoprotein inhibitor in a synergist bioassay with pyrethroids on one An. coluzzii populations from northern Nigeria. Genomic DNA extraction and SINE 200 PCR established that the Anopheles s. l. from Auyo, Jigawa State was An. coluzzii. Pre-exposure to verapamil followed by Permethrin results in 0.12% knockdown after 1 hour exposure and 10.7% mortality after 24 hours, which was higher than that for permethrin alone, but not statistically significant. This bioassay result reveals that ABC transporters possibly do not contribute to the resistance. 展开更多
关键词 DNA BIOASSAY PCR anopheles coluzzii INSECTICIDES and malaria
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Chemical Composition and Use of Momordica charantia L. and Hyptis spicigera Lam. Extracts as Mosquito Larvicides and Insect Growth Regulators against Malarial Vector, Anopheles gambiae Giles
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作者 David Ngadvou Simon Pierre Yinyang Danga +1 位作者 Bouladji Yonki Elias Nchiwan Nukenine 《Advances in Entomology》 2023年第2期47-62,共16页
Background: Mosquitoes are responsible of numerous vector-borne diseases and among these diseases is malaria which takes away lives of thousands of people, especially children of under five, particularly in Africa. To... Background: Mosquitoes are responsible of numerous vector-borne diseases and among these diseases is malaria which takes away lives of thousands of people, especially children of under five, particularly in Africa. To reduce the mortality and economic burdens of this deadly disease, synthetic insecticide has been of use to control its main vector, Anopheles gambiae. Due to adverse effects caused by these conventional products on non-target organisms and the environment, plants have been of first choice as they proved their effectiveness against mosquitoes and are said to be eco-friendly and relatively safer. Therefore, the aim of this study was to screen the phytochemicals and determine the larvicidal and adult emergence exhibitory activities of Momordica charantia and Hyptis spicigera leaves extracts against Anopheles gambiae larvae. Methods: The WHO standard protocol for larvicidal activity and IGRs was followed up and concentrations from 500 - 2000 ppm for larvicidal and 500 - 1500 ppm for IGRs were set up. Results: There was the presence of alkaloids, flavonoids, tannins, phenols, steroids, terpenoids and oils in Momordica charantia against alkaloids, taponins, tannins, phenols, steroids, terpenoids and oils in Hyptis spicigera. Methanol crude extract registered the lowest LC<sub>50</sub> value of 270.6 ppm followed by ethyl acetate and hexane fractions recorded the LC<sub>50</sub> of 742.1 and 756 ppm, respectively for larvicidal activity of Momordica charantia against LC<sub>50</sub> of 760 and 867.5 ppm for hexane fraction and methanol crude extract, respectively for Hyptis spicigera. Methanol crude extract and hexane fraction of both plants as well as the ethyl acetate fraction of M. charantia registered greater emergence inhibition with IE<sub>50</sub> values of 590.2, 842.3 and 982 ppm for methanol crude extract, hexane and ethyl acetate fractions of M. charantia, respectively against 901.7 and 873.2 for methanol crude extract and hexane fraction of H. spicigera, respectively. Conclusion: The toxicity and adult emergence inhibition may be associated with constituents in both plants that interfere with the normal neuronal, respiratory and endocrine systems functions. 展开更多
关键词 Momordica charantia Hyptis spicigera anopheles gambiae malaria EXTRACTS IGRS
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<i>Anopheles leesoni</i>Evans 1931, a Member of the <i>Anopheles funestus</i>Group, Is a Potential Malaria Vector in Cameroon
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作者 Edmond Kopya Cyrille Ndo +5 位作者 Landre Djamouko-Djonkam Leslie Nkahe Parfait Awono-Ambene Flobert Njiokou Charles Sinclair Wondji Christophe Antonio-Nkondjio 《Advances in Entomology》 2022年第1期99-109,共11页
<b><span style="font-family:Verdana;">Background:</span></b></span><span><span><span style="font-family:""><span style="font-family:Verd... <b><span style="font-family:Verdana;">Background:</span></b></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> Understanding the biology of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> malaria vector species is essential to planning effective and sustainable malaria control strategies in endemic countries. This study reported the implication of </span><i><span style="font-family:Verdana;">Anopheles leesoni </span></i><span style="font-family:Verdana;">in malaria transmission in Cameroon, Central Africa. </span><b><span style="font-family:Verdana;">Methods:</span></b><i> </i><span style="font-family:Verdana;">Mosquitoes were collected in three localities from May 2015 to March 2018 using electric aspirators and Centers for Disease Control light traps (CDC-LT). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i> <i><span style="font-family:Verdana;">sensu lato</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">.) mosquitoes were identified as species using polymerase chain reaction assay (PCR). Furthermore, </span><i><span style="font-family:Verdana;">Plasmodium falciparum</span></i><span style="font-family:Verdana;"> infection status was determined using the enzyme-linked</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">immunosorbent assay (ELISA) method. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A total of 12,744 </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes were collected by electric aspirator (N = 4844) and CDC-LT (N = 7900). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (86.95%)</span><i> </i></span><span style="font-family:Verdana;">was the major species and the main malaria vector in rural savannah and rural forest sites followed by </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">gambiae</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (13.05%)</span></span><span style="font-family:Verdana;"> whereas</span><span><span style="font-family:Verdana;"> in urban areas, </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> gambiae</span></i> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. was</span></span><span style="font-family:Verdana;"> by far the most abundant representing 91.45% of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes collected. Two members of the </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus</span></i><span style="font-family:Verdana;"> group were identified among 1389 analysed by PCR: 1307 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus sensu stricto</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.)</span><i> </i><span style="font-family:Verdana;">(94.10%) and 82 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i><span style="font-family:Verdana;"> (5.9%). </span><i><span style="font-family:Verdana;">Plasmodium falciparum </span></i><span style="font-family:Verdana;">infection rate was 21.04% in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus </span></i></span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">. For the first time, </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i> </span><span style="font-family:Verdana;">was found positive for </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i><span style="font-family:Verdana;"> (infection rate: 10.98%)</span></span><span style="font-family:Verdana;"> in Cameroon. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">A very high </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection rate was observed in this study in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i></span><span style="font-family:Verdana;">., highlighting its high implication in malaria transmission in Cameroon. Furthermore, the detection of </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i></span><span style="font-family:Verdana;"> calls for more attention towards this neglected vector species. 展开更多
关键词 anopheles funestus Group anopheles leesoni Plasmodium falciparum malaria Transmission Cameroon
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昆明按蚊(Anopheles Kunmingensis)的地理分布、生态习性、传疟作用及其防治研究 被引量:12
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作者 董学书 王学忠 +1 位作者 张国才 卢勇荣 《Zoological Research》 CAS CSCD 1990年第4期317-324,共8页
1986年—1988年对昆明按蚊(Anopheles kunmingensis Dong et Wang.)在云南的地理分布、生态习性、传疟作用等进行了研究。从地理分布、种群数量、嗜血习性、栖息习性、季节消长、自然感染率以及防治试验证实,昆明按蚊是云南省北纬24... 1986年—1988年对昆明按蚊(Anopheles kunmingensis Dong et Wang.)在云南的地理分布、生态习性、传疟作用等进行了研究。从地理分布、种群数量、嗜血习性、栖息习性、季节消长、自然感染率以及防治试验证实,昆明按蚊是云南省北纬24°以北,海拔1600米以上地区的重要传疟媒介。用常规的化学杀虫剂滞留喷洒,可有效地控制该蚊的密度及有关疟疾流行病学指数。 展开更多
关键词 昆明按蚊 传疟作用 生态 地理分布
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云南中华按蚊(An.sinensis)和微小按蚊(An.minimus)对五种杀虫剂的抗性调查 被引量:11
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作者 杜尊伟 顾云安 +1 位作者 卢勇荣 朱国君 《医学动物防制》 1995年第4期379-383,共5页
1992—1994年对云南省的思茅、景洪、潞西等20个县市、25个点的中华按蚊、微小按蚊对DDT、马拉硫磷(Malathion)、杀螟硫磷(Fenitrothion)、二氯苯醚菊酯(Permethrin)和溴氰菊酯(Deltamethrin)的抗性情况进行了调查,结果共获104个数据,... 1992—1994年对云南省的思茅、景洪、潞西等20个县市、25个点的中华按蚊、微小按蚊对DDT、马拉硫磷(Malathion)、杀螟硫磷(Fenitrothion)、二氯苯醚菊酯(Permethrin)和溴氰菊酯(Deltamethrin)的抗性情况进行了调查,结果共获104个数据,中华按蚊对DDT显示出较高而普遍的抗性,对Permethrin和Deltamethrin分别有中度和高度抗性出现;整个调查结果以Malathion和Fenitrothion最敏感。 展开更多
关键词 中华按蚊 微小按蚊 杀虫剂 抗性
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Spatio-temporal distribution of malaria vectors(Diptera:Culicidae) across different climatic zones of Iran 被引量:10
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作者 Ahmad Ali Hanafi-Bojd Shahyad Azari-Hamidian +1 位作者 Hassan Vatandoost Zabihollah Charrahy 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2011年第6期498-504,共7页
Malaria is a main vector-borne public health problem in Iran.The last studies on Iranian mosquitoes show 31 Anopheles species including different sibling species and genotypes,eight of them are reported to play role i... Malaria is a main vector-borne public health problem in Iran.The last studies on Iranian mosquitoes show 31 Anopheles species including different sibling species and genotypes,eight of them are reported to play role in malaria transmission.The objective of this study is to provide a reference for malaria vectors of Iran and to map their spatial and temporal distribution in different climatic zones.Shape files of administrative boundaries and climates of Iran were provided by National Cartographic Center.Data on distribution and seasonal activity of malaria vectors were obtained from different sources and a databank in district level was created in Excel 2003, inserted to the shape files and analyzed by ArcGIS 9.2 to provide the maps.Anopheles culicifacies Giles s.l.,Anopheles dthali Patton,Anopheles fluviatilis James s.L,Anopheles maculipennis Meigen s.L,Anopheles sacharovi Favre,Anopheles stephensi IJston,and Anopheles superpictus Grassi have been introduced as primary and secondary malaria vectors and Anopheles puicherrimus Theobald as a suspected vector in Iran.Temporal distribution of anopheline mosquitoes is restricted to April_December in northern Iran,however mosquitoes can be found during the year in southern region.Spatial distribution of malaria vectors is different based on species,thus six of them(except for Anopheles maculipennis s.l.and Anopheles sacharovi) are reported from endemic malarious area in southern and southeastern areas of Iran.The climate of this part is usually warm and humid,which makes it favorable for mosquito rearing and malaria transmission.Correlation between climate conditions and vector distribution can help to predict the potential range of activity for each species and preparedness for malaria epidemics. 展开更多
关键词 anopheles malaria DISTRIBUTION GIS Iran
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Environmental characteristics of anopheline mosquito larval habitats in a malaria endemic area in Iran 被引量:2
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作者 Moussa Soleimani-Ahmadi Hassan Vatandoost +4 位作者 Ahmad-Ali Hanafi-Bojd Mehdi Zare Reza Safari Abdolrasul Mojahedi Fatemeh Poorahmad-Garbandi 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2013年第7期510-515,共6页
Objective:To determine the effects of environmental parameters of lanal habitats on distribution and abundance of anopheline mosquitoes in Rudan county of Iran.Methods:This cross-sectional study was conducted during t... Objective:To determine the effects of environmental parameters of lanal habitats on distribution and abundance of anopheline mosquitoes in Rudan county of Iran.Methods:This cross-sectional study was conducted during the mosquito breeding season from February 2010 to October 2011. The anopheline larvae were collected using the standard dipping method,the specimens were identified using a morphological-based key.Simultaneously with larvay collection,environmental parameters of the larval habitats including water current and turbidity,sunlight situation,and substrate type of habitats were recorded.Water samples were taken from breeding sites during lanal collection.Before collection of samples,the water temperature was measured.The water samples were analysed for turbidity,conductivity,total alkalinity,total dissolved solid.pH and ions including chloride,sulphate,calcium,and magnesium.Statistical correlation analysis and ANOVA test were used to analyze the association between environmental parameters and larval mosquito abundance.Results:In total 2 973 lanae of the genus Anopheles were collected from 25 larval habitats and identified using morphological characters.They comprised of six species: An.dthali(53.21%),An.stephensi(24.22%),An.culicifacies(14.06%),An.superpictus(4.07%),An. turkhudi(3.30%),and An.apoci(1.14%).The most abundant species was An.dthali which were collected from all of the study areas.Larvae of two malaria vectors.An.dthali and An.stephensi, co-existed and collected in a wide range of habitats with different physico-chemical parameters. The most common larval habitats were man-made sites such as sand mining pools with clean and still water.The anopheline mosquitoes also preferred permanent habitats in sunlight with sandy substrates.The results indicated that there was a significant relationship between mean physicochemical parameters such as water temperature,conductivity,total alkalinity,sulphate,chloride, and mosquito distribution and abundance.Conclusions:The results of this study showed a correlation between certain environmental parameters and mosquito lanae abundance,and these parameters should be considered in planning and implementing larval control programs. 展开更多
关键词 anopheles LARVAL HABITATS malaria Rudan Iran
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Bio-ecology of malaria vectors in an endemic area,Southeast of Iran 被引量:2
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作者 Masoud Yeryan Hamid Rreza Basseri +3 位作者 Ahamd Ali Hanafi-Bojd Ahmad Raeisi Hamideh Edalat Reza Safari 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第1期29-35,共7页
Objective:To determine some bio-ecological aspects of malaria vectors in Jask County,where is targeted for malaria elimination in the national program.Methods:Mosquitoes were collected monthly during 2013-2014 using d... Objective:To determine some bio-ecological aspects of malaria vectors in Jask County,where is targeted for malaria elimination in the national program.Methods:Mosquitoes were collected monthly during 2013-2014 using different collection methods.Subsequently,ELISA test was used to detect the human blood index of mosquitoes.The susceptibility status of Anopheles stephensi was evaluated against the diagnostic dosages of seven WHO recommended insecticides.Results:A total of 3 650 female and 4 736 Anopheles larvae were collected including Anopheles stephensi,Anopheles culicifacies s.l.,Anopheles dthali.Anopheles fluviatilis s.l.,Anopheles moghulensis and Anopheles turkhodi species.Anopheles stephensi was the dominant collected species on human baits and indoors with high rate of unfed and gravid specimens in internal and external window traps.Human blood index was calculated as 14.3%for this species.It was also found to be resistant to DDT and Dieldrin.Conclusions:The collected species had a wide range of habitats,and resting behaviors.With regarding to the presence of most important malaria vectors in Jask.control of the disease may be so complicated;as based on the weather condition it can be transmitted during the whole year,except for cold months.With this strong potential of transmission,existing population movements in the area may lead to imported cases of malaria and local outbreak(s).So,more specific studies on malaria vectors in high risk areas of Jask County are recommended. 展开更多
关键词 malaria ECOLOGY anopheles STEPHENSI INSECTICIDE resistance Southern Iran
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